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951.
No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU, type 1) or North American (NA, type 2) viruses, respectively. Two amplicons (one from type 1 and one from type 2) were used to analyze the PRRSV open reading frame 7 (ORF7) sequence. The nucleotide sequences of type 1 PRRSV ORF7 had identities between 96.1% and 98.4% with PRRSVs from domestic pigs in Korea. The sequences of type 2 PRRSV ORF7 had identities of 100% with the PRRSV strain VR-2332, which was prototypic North American strain. These results show that PRRSVs are present in wild boars in Korea, and effective PRRSV surveillance of the wild boar population might therefore be useful for disease control.  相似文献   
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Although urban growth boundaries (UGBs) are increasingly used as a containment policy to promote contiguous and compact urban forms, little is known about how UGBs affect development within the designated area or how development is displaced to other cities. This study analyzes the UGB's containment and spillover effects, focusing on the case of the Portland metropolitan area where UGBs have long been implemented. This is accomplished by employing a spatial market disequilibrium framework in which explicit consideration is given to the containment and spillover processes when under the influences of UGBs. The findings suggest that UGBs can promote development within the boundaries, but that cooperation with neighboring municipalities is necessary to effectively attain the policy goals.  相似文献   
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The objective of this study is to examine the feasibility of using visible light to form gels from polysaccharide precursors. Hydrogel formation by visible light irradiation would be very beneficial because visible light is a benign light source and ready available when compared with other light sources such as UV. Dextran-methacylate was synthesized and photocrosslinked using (−)-riboflavin as a photoinitiator and L-arginine as a co-initiator under the visible light. The effect of various concentrations of (−)-riboflavin and L-arginine on the photo-crosslinking of dextran-methacrylate hydrogel was investigated. The fabricated hydrogel was characterized by FT-IR and SEM. The photoinitiator [(−)-riboflavin] and co-initiator (L-arginine) as well as dextran precursor are completely biocompatible. The optimum condition for the biocompatible dextran-based hydrogel formation under the harmless light source (visible light) was elucidated in this study. In general, the (−)-riboflavin, 0.01–0.5 %, and L-arginine, 5–20 % of the weight of dextran-methacrylate were the best condition in forming dextran-based hydrogels under the visible light. The three-dimensional hydrogel structure was verified by SEM morphology of swollen hydrogels. Photocrosslinking under the visible light source would enlarge the applications of this type of photocrosslinking in the biomedical area (e.g., eyes or other light-sensitive organs).  相似文献   
956.
Fishways have historically been constructed to restore and preserve the ecological connectivity for fish in fragmented rivers. However, the fishways are often selective on species due to different size and swimming capacity. As the proportion of dammed rivers is still increasing, there is a growing need for more information on wild fish and their migration potential. In this study, we compared the swimming capacity of wild caught brown trout (Salmo trutta) and European grayling (Thymallus thymallus) until the fish were exhausted in a critical swimming speed (Ucrit) test, under three different naturally occurring stream temperatures in Norway: 1.7, 5.5 and 10°C. The results indicated that trout swim better at the warmer temperatures than at colder temperatures. The grayling showed consistent swimming patterns with little variation across all tested temperatures. The results therefore signify the need to have operational fishways already early in the spring when the grayling migration starts and highlight the need for more studies on fish migration abilities across a wider range of species and seasons.  相似文献   
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Culturing the longsnout seahorse, Hippocampus reidi, remains challenging because unlike most cultured seahorse species, Artemia is not an optimal first feed. Ideally, more nutritious live feeds such as wild plankton or copepods should be used, but are not always readily available. Therefore, facilities commonly utilize enriched rotifers. Numerous feed enrichment products exist, including Dan's Feed Artemia Enrichment®, (Dan's Feed) which is a blend of commercially available enrichment products. The aim of this study was to assess the survivorship, growth, and condition factor (CF) of 0–28 DPR (days post release) H. reidi fed with rotifers and Artemia enriched with Dan's Feed. This enrichment was evaluated against (1) rotifers and Artemia enriched with live alga (Isochrysis galbana), and (2) wild plankton and Artemia enriched with Dan's Feed. At 28 DPR, in terms of survivorship and growth, seahorses fed with rotifers and Artemia enriched with Dan's Feed had a lower CF and outperformed those fed rotifers and Artemia enriched with I. galbana. Seahorses fed with rotifers and Artemia enriched with Dan's Feed performed on par with those fed with wild plankton and Artemia with no significant difference in CF. These findings demonstrated that the culture of H. reidi may be feasible with rotifers and Artemia enriched with Dan's Feed .  相似文献   
960.
We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI with hUCB-MSCs can improve locomotor functions, and suggest that increased levels of BDNF, NGF and NT-3 in the injured spinal cord were the main therapeutic effect.  相似文献   
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