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Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was conducted to determine whether or not there are interstrain or site-dependent differences in the gene expression profiles of skeletal muscles in SJL/J and A/J mice as dysferlinopathy models. Upon analysis by qRT-PCR, SJL/J mice showed a trend of increased gene expression level of uncoupling protein 2 in the rectus femoris and longissimus lumborum at 30 weeks of age when dystrophic lesions became histopathologically pronounced. Heme oxygenase 1 and S100 calcium binding protein A4 were upregulated in the rectus femoris, longissimus lumborum and abdominal muscles, in which dystrophic lesions occur more commonly in SJL mice. The gene expression levels of heat shock protein 70 in most muscles of A/J mice were lower than those of BALB/c mice as control. SJL/J mice exhibited a marked lowering of decay-accelerating factor 1/CD55 gene expression level in all studied muscles except for the heart at all ages compared with that of BALB/c mice. This study showed that there were some interstrain differences in the gene expres sion profiles of skeletal muscles between SJL/J and A/J mice. Further investigation is required to reveal whether these alterations of the expression levels are the cause of dystrophic changes or occur subsequent to muscle damage. 相似文献
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Toshiko Furukawa Kinji Ushiyama Kunihei Kishi 《Journal of General Plant Pathology》2008,74(2):117-119
A leaf spot disease of scarlet sage (Salvia splendens Sellow ex J.A. Shultes) found in Kanagawa and Tokyo prefectures was demonstrated to be caused by Corynespora cassiicola (Berk. and Curt.) Wei based on inoculation experiments, and morphological identification of the pathogenic fungus. Isolates
of C. cassiicola from cucumber, green pepper, and hydrangea were also pathogenic to scarlet sage leaves. Although the isolates from cucumber,
green pepper, and hydrangea were pathogenic to scarlet sage leaves, the scarlet sage isolate was not pathogenic to cucumber,
green pepper, hydrangea, eggplant, tomato or soybean. 相似文献
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Tsujimoto S Okuda K Okuda H Une Y Nomura Y Shirota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(3):329-331
A 3-month-old female tortoise-shell cat showing azotemia died with a marked swollen abdomen. Necropsy revealed a huge perirenal cyst (8.5 x 6.0 x 4.5 cm) on the ventral aspect of the right kidney. The cyst was filled with the pellucid yellow fluid with a smell of urine. The lumen was connected with irregularly dilated renal pelvis by a narrow channel passing through the renal parenchyma. The cyst was lined by epithelial cells and its wall was consisted of collagen fibers and smooth muscle cells as that of the renal pelvis and ureter. Renal parenchyma adjacent to the channel showed interstitial infiltration of the lymphoid cells. The cyst was a diverticulum of the renal pelvis due to an impaired development. 相似文献
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Ciprian Pîrvu Hiroaki Yoshida Masahiro Inayama Motoi Yasumura Kinji Taki 《Journal of Wood Science》2000,46(3):193-201
In past years high priority was given to developing a seismic design for wood structures, including research on the response of wood structures to earthquakes. In this study a new type of portal frame with relatively large span was developed for the traditional Japanese wooden houses with large openings at the front to strengthen the structure. Stainless steel plates coated with zinc and glued with epoxy adhesives on laminated veneer lumber (LVL) members, composed of Douglas fir veneer and bonded with phenolformaldehyde resin, were used. The connection between the frame's beam and columns and between the columns and groundsills was mechanical, with bolts. The subject of this research was to analyze strength properties and failure behavior of glued LVL metal joints used as structural components and to evaluate the response of LVL portal frames under cyclic lateral loading. The results show that portal frames using glued LVL metal plates have a good multiplier for the shear walls and may be applied to traditional Japanese structures. The equivalent viscous damping provided good energy dissipation in the frames. The joints displayed good mechanical behavior during tests; moreover, the structures demonstrated high strength, stiffness, and ductility, which are necessary for a seismic design.Part of this paper was presented at the 47th annual meeting of the Japan Wood Research Society, Kouchi, April 1997; and at the 5th world conference on timber engineering. Montreux, Switzerland, August 1998 相似文献
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Kyohei Yasuno Haruka Sakashita Ryosuke Kobayashi Saori Araki Rio Saito Mariko Shirota Junichi Kamiie Kinji Shitora 《Journal of toxicologic pathology》2013,26(2):203-208
Membranous glomerulonephropathy can be experimentally induced in rats, but spontaneous
cases have been rarely reported. In this report, we present a typical case of spontaneous
membranous glomerulonephropathy in a rat. A male Hatano low-avoidance (LAA) strain rat had
a tumor mass on the right auricle, and was sacrificed at 41 weeks of age. Urinary
screening by reagent strips revealed intense proteinuria. Histological tests revealed
frequent presence of irregularly sized eosinophilic hyaline materials on the capillary
wall and in the mesangium of renal glomeruli. Immunofluorescence revealed granular
deposits of IgG, IgM, and C3 in the glomeruli. Subepithelial dense deposits were observed
by electron microscopy accompanied by podocyte foot process effacement and occasional
irregular thickening of the glomerular basement membrane. The rat also developed chronic
lymphocytic pancreatitis, and the tumor mass on the right auricle was diagnosed as a
fibrosarcoma. Screening tests for antibodies against major infectious agents and
antinuclear antibody were negative. Western blot and indirect immunofluorescence analyses
suggested the presence of an autoantibody against the pancreatic component. The
glomerulopathy was considered an early stage of membranous glomerulonephropathy. 相似文献
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Eka Mulya Alamsyah Liu Chang Nan Masaaki Yamada Kinji Taki Hiroaki Yoshida 《Journal of Wood Science》2007,53(1):40-46
To estimate the potential bonding performance of bonded wood products from tropical fast-growing tree species, a study on
the bondability of Paraserianthes falcataria L. Nielsen, Pinus merkusii Jungh et. De. Vriese, and Acacia mangium Willd from Indonesia was conducted. Two-ply laminations were produced using polyvinyl acetate emulsion (PVAc), urea formaldehyde
(UF), resorcinol formaldehyde (RF), and water-based polymer isocyanate (API) adhesives. In order to determine the bonding
performance, the block-shear test was applied according to the Japanese Agricultural Standard for structural glued laminated
timber under normal conditions and after accelerated-aging treatments. To support this study, the wettability of each wood
species was also investigated through contact-angle measurement. The results showed that the bonding performance of low-density
P. falcataria was better than that of medium-density P. merkusii and medium-density A. mangium, while the bonding performance of medium-density P. merkusii was better than that of medium-density A. mangium. Furthermore, compared with A. mangium, the small contact angle and good wettability in P. falcataria and P. merkusii result in better adhesion and more intimate contact between the wood surfaces and adhesive. 相似文献
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Sakurada Y Shirota M Mukai M Inoue K Akahori F Watanabe G Taya K Shirota K 《The Journal of reproduction and development》2007,53(4):937-943
We have previously shown that 3,3',4,4',5-pentachlorobiphenyl (PCB-126) vertically transferred from dams potentially exerts a direct effect on the ovaries of offspring and adversely affects female puberty. To investigate its toxicological targets in ovarian tissues, mRNAs encoding representative peptides that regulate follicular development in granulosa cells, theca cells, and oocytes were quantified using ovaries collected on postnatal days (PND) 5, 15, and 24 from the offspring of dams administered oral doses of 0, 1 or 3 microg/kg PCB-126 starting 2 weeks prior to mating and continuing until 20 days after delivery. Quantification using the real-time RT-PCR method revealed that PCB-126 lowered the amounts of mRNAs that encoded the inhibin alpha- and inhibin/activin beta A-subunits from PND 15 onwards; the amounts of mRNAs for inhibin/activin beta B-subunit, follicle-stimulating hormone (FSH) receptor, and aromatase on PND 15; and the amounts of luteinizing hormone receptor mRNA on PND 24 compared with those of the age-matched controls. In contrast, no differences were noted for mRNAs encoding c-kit, growth differentiation factor-9, bone morphogenetic protein-15, or kit ligand for any of the age groups examined. The serum FSH level on PND 24 was higher than that in the control. Since the earliest effects on the mRNAs in the rat ovaries were observed in those expressed in the granulosa cells of the growing follicles after the antral follicles had developed, molecules in granulosa cells but not in oocytes during the early stages of the antral follicles might be the primary targets of vertically transferred PCB-126. 相似文献
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K Yasuno H Sakashita S Araki R Kobayashi Y Sakurada M Shirota J Kamiie K Shirota 《Journal of toxicologic pathology》2012,25(2):175-178
This report describes a spontaneous hybrid cyst in a Sprague-Dawley (SD) rat. A 52-week-old, male SD rat had a cutaneous cyst on the left mystacial pad. Histologically, the cyst wall showed infundibular differentiation with keratohyalin granules in the granular layer and matrical differentiation comprising basaloid epithelial cells with trichohyalin granules. The cyst cavity was filled with lamellar, flaky keratin and aggregates of shadow cells. Immunohistochemically, the infundibular-type epithelium was positive for cytokeratin (CK) AE1/AE3, CK KL1 and CK14 but negative for CK15, whereas the matrical-type epithelium was negative for all four CK isoforms examined. These immunohistochemical properties of the infundibular- and matrical-type epithelia were similar to those of the infundibulum and inferior segment of normal hair follicles, respectively. Based on these findings, the cyst was diagnosed as a hybrid cyst, comprising more than one type of cyst arising from various parts of the pilosebaceous unit. 相似文献