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21.
Hiroshi SUNAHARA Yoko FUJII Keisuke SUGIMOTO Takuma AOKI Gou SUGAHARA Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):95-99
A two-month-old female Chihuahua was diagnosed as severe pulmonary valvular
stenosis (PS). Although balloon valvuloplasty (BV) was successfully performed, restenosis
was observed 19 months after the procedure. Euthanasia was chosen due to low output
syndrome during the surgical repair attempted when the dog was 5 years old. Postmortem
examination revealed markedly thickened pulmonary valve due to the increase of
extracellular matrix which might be produced by increased α smooth muscle actin-positive
myofibroblasts. The thickening of the valve was associated with restriction of the valve’s
motion, resulting in restenosis in the present case. This is the first case report
documented histopathological and immunohistochemical findings of the restenotic pulmonary
valve in dogs with PS after BV. 相似文献
22.
Kyohei Ago Go Sugahara Kinji Shirota Yasushi Kurata 《Journal of toxicologic pathology》2015,28(4):237-241
Glomerular lesions including membranoproliferative glomerulonephritis occur spontaneously in aged mice, but they are rare in young animals. In our laboratory, spontaneous glomerulonephritis was observed in an 8-week-old male Crj:CD1 (ICR) mouse. Macroscopically, the bilateral kidneys were discolored, but no edema or ascites was observed. Glomerular lesions were characterized by a thickening of capillary walls, a double-contoured basement membrane and mesangial expansion due to increased amounts of matrix. Ultrastructurally, mesangial interposition in the capillary wall and subendothelial deposition of basement membrane-like material were observed. No evidence of immune complex deposition or amyloid was found. On the basis of the observed clinical pathology and histopathology, a secondary form of glomerular lesion was excluded. The glomerular lesion was compatible with glomerulonephritis in a young Crj:CD1 (ICR) mouse. 相似文献
23.
Go SUGAHARA Ichiro NAITO Yuichi MIYAGAWA Takaaki KOMIYAMA Naoyuki TAKEMURA Ryosuke KOBAYASHI Takayuki MINESHIGE Junichi KAMIIE Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1175-1178
The renal biopsy tissue from a 9-month-old, male Pyrenean Mountain dog with
renal disorder and severe proteinuria was examined. Ultrastructural examination revealed
multilaminar splitting and fragmentation of the glomerular basement membrane (GBM) and
diffuse podocyte foot process effacement. Immunofluorescent staining for α(IV) chains
revealed presence of α5(IV) and complete absence of α3(IV) and α4(IV) chains in the GBM.
Immunohistochemistry also revealed decreased and altered expression of nephrin and podocin
in the glomeruli compared with normal canine glomeruli. These results suggested that the
glomerular disease of the present case might be consistent with canine hereditary
nephropathy resembling human Alport syndrome caused by genetic defect of type IV collagen,
and indicated possible contribution of podocyte injury to severe proteinuria in this
case. 相似文献
24.
Kobayashi R Yamano S Tanaka K Kamiie J Shirota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1347-1349
A 10-month-old cat was diagnosed with congenital subvalvular aortic stenosis. To resolve its hypoxia, oxygen therapy was administered a couple of times a week during two months. The oxygen partial pressure in the chamber was maintained between 30 and 35%, and the time for one procedure was 12-24 hr. The animal died due to severe respiratory failure. At necropsy, the lungs were voluminous and had a rubbery texture. Histologically, large type II pneumocytes with occasional atypia had diffusely proliferated within the lungs. Interstitial fibrosis was not observed, although some alveolar septa were thickened along with fibrinous exudates and neutrophilic infiltration. The histology of these lesions was consistent with diffuse alveolar damage (DAD), which might have been partially due to oxygen toxicity. 相似文献
25.
Takao Kobayashi Yuuri Hirooka Keiko T. Natsuaki Yuusuke Kawashima Kinji Ushiyama 《Journal of General Plant Pathology》2005,71(2):124-126
New perennial canker diseases of Abies veitchii and Acer crataegifolium are described. Pathogenicity of the causal fungus was confirmed on stems of young Abies and Acer trees using two monoascospore isolates from the perithecia from the two tree species. The causal fungus, producing Nectria-state-teleomorph and Cylindrocarpon-anamorph, was identified as Nectria castaneicola. It was then transferred to the genus Neonectria, as N. castaneicola (W. Yamam. et Oyasu) Tak. Kobay. et Hirooka, comb. nov. followed by the recent concept of Nectriaceae. 相似文献
26.
Haishima A Kawakami Y Mizuno S Kageyama T Muto M Suzuki T Inoue K Shirota K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(12):1137-1140
Renal allograft transplantation was performed in four beagles. Immunosuppressive treatment using cyclosporine, mizoribin and prednisolone was continued from Day 5 pre- until Day 20 post-transplantation. Between Days 28 and 32 post-transplantation, an abrupt elevation of the serum creatinine values followed by the development of uremia was seen in all recipients. Histopathology of the allografts examined between Days 28 and 37 revealed edema, necrosis, hemorrhage and severe diffuse interstitial cellular infiltration as well as tubulitis. Glomerular changes notably included swelling of the tufts due to hypercellularity, which was consistent with transplant glomerulitis. The intrarenal arteries exhibited fibrinoid necrosis of the walls and intimal or transmural cellular infiltration. These renal lesions were consistent with those of acute vascular and interstitial rejection in humans. 相似文献
27.
Adachi S Yamada S Takatsu Y Matsui H Kinoshita M Takase K Sugiura H Ohtaki T Matsumoto H Uenoyama Y Tsukamura H Inoue K Maeda K 《The Journal of reproduction and development》2007,53(2):367-378
Metastin/kisspeptin, the KiSS-1 gene product, has been identified as an endogenous ligand of GPR54 that reportedly regulates GnRH/LH surges and estrous cyclicity in female rats. The aim of the present study was to determine if metastin/kisspeptin neurons are a target of estrogen positive feedback to induce GnRH/LH surges. We demonstrated that preoptic area (POA) infusion of the anti-rat metastin/kisspeptin monoclonal antibody blocked the estrogen-induced LH surge, indicating that endogenous metastin/kisspeptin released around the POA mediates the estrogen positive feedback effect on GnRH/LH release. Metastin/kisspeptin neurons in the anteroventral periventricular nucleus (AVPV) may be responsible for mediating the feedback effect because the percentage of c-Fos-expressing KiSS-1 mRNA-positive cells to total KiSS-1 mRNA-positive cells was significantly higher in the afternoon than in the morning in the anteroventral periventricular nucleus (AVPV) of high estradiol (E(2))-treated females. The percentage of c-Fos-expressing metastin/kisspeptin neurons was not different between the afternoon and morning in the arcuate nucleus (ARC). Most of the KiSS-1 mRNA expressing cells contain ERalpha immunoreactivity in the AVPV and ARC. In addition, AVPV KiSS-1 mRNA expressions were highest in the proestrous afternoon and lowest in the diestrus 1 in females and were increased by estrogen treatment in ovariectomized animals. On the other hand, the ARC KiSS-1 mRNA expressions were highest at diestrus 2 and lowest at proestrous afternoon and were increased by ovariectomy and decreased by high estrogen treatment. Males lacking the surge mode of GnRH/LH release showed no obvious cluster of metastin/kisspeptin-immunoreactive neurons in the AVPV when compared with high E(2)-treated females, which showed a much greater density of these neurons. Taken together, the present study demonstrates that the AVPV metastin/kisspeptin neurons are a target of estrogen positive feedback to induce GnRH/LH surges in female rats. 相似文献
28.
The effects of curing time at room temperature and methanol extracts from Acacia mangium on the curing behavior of resorcinol formaldehyde (RF) adhesive were examined by using the thermomechanical analysis spring
method. For a specimen that was cured for 3 months at room temperature, the relative elasticity (E
r) curve did not change to a hard glass state from room temperature to 200°C and the adhesive had cured completely. The initial
temperature of the reactive zone for chemical and mechanical changes was 15° and 25°C higher than that for the control when
10 and 15 parts by weight methanol extract was added to the liquid adhesive, respectively. It appears that the extractives
of A. mangium in RF adhesive interferes with the chemical cure of the adhesive. It is suggested that a combination of curing time and sweeping
by methanol on the laminae surface can improve the bonding performance of A. mangium laminates bonded with RF at room temperature. 相似文献
29.
Imai A Nishita T Ichihara N Shirota K Orito K 《Veterinary immunology and immunopathology》2012,149(1-2):108-111
Xylitol is used as a sugar substitute in food products. Dogs have been reported to experience lethal liver injury after accidental ingestion of xylitol. Because liver injury may be a serious consequence of canine immune-mediated reactions, antibodies produced against xylitol may attack the liver. Therefore, in the present study, we evaluated whether binding sites for xylitol antibodies are located at the liver or not. Anti-xylitol antibodies were generated by immunization of rabbits with a xylose-bovine serum albumin conjugate. Immunohistological examination showed that binding sites for the anti-xylitol antibodies were located in the hepatic arteries and the portal veins. Western blotting analyses by using a canine liver homogenate showed 4 protein bands with different molecular weights which reacted with anti-xylitol antibodies. Therefore, binding of anti-xylitol antibodies to the vessels may be the first step in an immune-mediated pathogenic response in xylitol toxicity. Further studies are necessary to determine the effects of anti-xylitol antibodies on the liver in the pathogenesis of xylitol toxicity. 相似文献
30.
Takayuki MINESHIGE Go SUGAHARA Tamio OHMURO Junichi KAMIIE Kinji SHIROTA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):739-742
A 12-year-old mixed-breed neutered female dog was referred with cutaneous tumors at the
left auricle. Histologically, the cutaneous tumor located in the dermis comprised numerous
clefts and cavernous channels lined by neoplastic endothelial cells with no erythrocytes.
Bone tissue without direct contact with neoplastic cells was seen in the well-developed
stromal connective tissue. The neoplastic endothelial cells exhibited mild to moderate
atypia. Immunohistochemically, neoplastic cells were positive for vimentin and negative
for cytokeratin and factor VIII-related antigen. Basement membrane around the neoplastic
lumens was positive for laminin in a linear or granular pattern. Ultrastructural
examination revealed discontinuous basement membrane beneath the tumor cells.
Histopathological features of this case were consistent with lymphangiosarcoma, and
stromal ossification was characteristic. 相似文献