Percentage of Ubiquitinated Spermatozoa does not Correlate with Fertilizing Capacity of Thawed Bovine Semen     

I Rodríguez‐Lozano  A Ávalos‐Rodríguez  H Castillo‐Juárez  F Borderas‐Tordesillas  JJ Roa‐Vidal  AM Rosales‐Torres 《Reproduction in domestic animals》2014,49(1):27-31

In the spermatozoa of some species, the ubiquitin–proteasome system detects altered proteins and tags them for elimination by the proteasome. In some species' ejaculates, a high proportion of ubiquitinated spermatozoa (i.e. those having ubiquitin bound to the altered or damaged membrane proteins) has been related to infertility. The aim of this study was to assess whether the percentage of ubiquitinated spermatozoa relates to fertility of dairy bulls and whether ubiquitination increases during protein remodelling that occurs during in vitro spermatic capacitation. Thirty‐two frozen semen straws from four high‐fertility (ReproMax^®) and four normal‐fertility (Normal) Holstein‐Friesian sires were evaluated. Ubiquitinated and capacitated spermatozoa were quantified by sperm ubiquitin tag immunoassay and chlortetracycline stain, respectively. Fertilizing capacity of sires was assessed by in vitro fertilization. No differences were found between Normal and ReproMax^® sires with regard to the observed percentage of ubiquitinated spermatozoa (42.97 ± 3.69% and 49.68 ± 9.27%, respectively; p > 0.05). Additionally, no differences were found in the percentage of ubiquitinated spermatozoa as a consequence of spermatic capacitation in either Normal (42.97 ± 3.69% before capacitation vs 44.67 ± 7.5% after; p > 0.05) or ReproMax^® sires (49.68 ± 9.27% before vs 45.05 ± 7.51% after; p > 0.05). The percentage of ubiquitinated spermatozoa in a thawed sperm samples did not correlate with its in vitro fertilizing capacity; thus, this assay does not prove useful to detect in vivo fertility differences between sires. Additionally, protein degradation occurring during remodelling of the spermatozoon plasma membrane during the capacitation process does not seem to involve the ubiquitin–proteasome system.  相似文献   

Seeking Mathematical Strategies in Sperm Function Analysis: Between Scylla and Charybdis?     

AM Petrunkina  A-R Günzel-Apel  E Töpfer-Petersen 《Reproduction in domestic animals》2003,38(2):125-133

During the last decades, essential progresses in reproductive biotechnology were achieved, implying development of special spermatological techniques. The major problem was to set up simple, rapid, precise and adequate evaluation methods. The key aspect to be considered in all assays of sperm fertilizing function is capacitation. As not all spermatozoa respond to fertilizing conditions in a similar manner, it seems to be logical to assess samples via their response to these specific conditions. For the spermatological practice, the sensitivity of methodology for assessment and analysis of data with respect to differences in individual response, in heterogeneity of the population, and proper temporal characterization of the response is crucial for the improvement of evaluation procedures. Currently, most used statistical analytical tools in spermatology do not always fulfil these essential sensitivity requirements. We structured our paper concerning different fields of mathematical science (distribution analysis, fractal geometry, functional approximation and differentiation) related to the modern insights in sperm function analysis. The spectrum of methods we are going to review in this paper is restricted to basic ideas to illustrate how the accuracy and sensitivity of sperm evaluation assays may be improved by applying adequate elementary tools of the mathematical analysis.  相似文献   

Dicoumarol toxicity in cattle associated with ingestion of silage containing sweet vernal grass (Anthoxanthum odoratum)     

DJ RUNCIMAN  AM LEE  KFM REED  JR WALSH 《Australian veterinary journal》2002,80(1):28-32

A diagnosis of dicoumarol toxicity in a herd of Friesian cattle was made following investigation of the deaths of three mature cows and eleven yearling heifers. Affected stock had been fed wrapped, bailed silage containing approximately 90% sweet vernal grass ( Anthoxanthum odoratum ). Sweet vernal grass contains coumarin, which can be converted to dicoumarol, a vitamin K antagonist, through the action of moulds. Most deaths were preceded by lethargy, severe anaemia and subcutaneous and internal haemorrhage. Dicoumarol toxicosis was suspected based on clinical signs, necropsy findings and prolonged prothrombin and activated partial thromboplastin times. Dicoumarol analysis of blood from affected animals and silage confirmed the diagnosis.  相似文献   

An attempt to prevent acute toxoplasmosis in macropods by vaccination with Hammondia hammondi     

GL REDDACLIFF  SJ PARKER  JP DUBEY  PJ NICHOLLS  AM JOHNSON  DW COOPER 《Australian veterinary journal》1993,70(1):33-35

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Successful treatment of a pancreatic pseudocyst by omentalisation in a dog     

RM Jerram  CG Warman  ESS Davies  MC Robson  AM Walker 《New Zealand veterinary journal》2013,61(4):197-201

CASE HISTORY A 3-year-old male Labrador retriever was presented with a history of dietary indiscretion followed by vomiting and abdominal pain.

CLINICAL FINDINGS AND TREATMENT: Abdominal ultrasonography revealed the presence of a fluid-filled cystic structure in the region of the pancreas. Flocculent, dark fluid was drained from the cystic structure during exploratory celiotomy prior to resection of the lateral cystic wall. Omentum was sutured into the cystic cavity to provide drainage and enhance immune response. A jejunostomy feeding tube was placed. Post-surgical care consisted of antibiotic therapy. The dog was clinically normal at 1 and 7 months post-operatively and the pancreas appeared normal on final ultrasonographic examination.

CLINICAL RELEVANCE: This case describes the use of omentum to provide physiological drainage of a pancreatic pseudocyst. This surgical technique may help clinicians manage patients with this condition in the future.  相似文献   

Thrombotic Microangiopathy,Hemolytic Uremic Syndrome,and Thrombotic Thrombocytopenic Purpura Following Hump-nosed Pit Viper (Genus: Hypnale) Envenoming in Sri Lanka     

RMMK Namal Rathnayaka  PE Anusha Nishanthi Ranathunga  Senanayake AM Kularatne 《The Forestry Chronicle》2019,30(1):66-78

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Reduction in the mRNA expression of sVEGFR1 and sVEGFR2 is associated with the selection of dominant follicle in cows          下载免费PDF全文

PV Ortega Serrano  A Guzmán  CG Hernández–Coronado  H Castillo‐Juárez  AM Rosales‐Torres 《Reproduction in domestic animals》2016,51(6):985-991

The vascular endothelial growth factor (VEGF) is essential for follicular development by promoting follicular angiogenesis, as well as for the proliferation and survival of granulosa cells. The biological effects of VEGF are regulated by two membrane receptors, VEGFR1 and VEGFR2, and two soluble receptors, sVEGFR1 and sVEGFR2, which play an antagonistic role. Thus, the objective of this study was to identify the mRNA expression pattern of total VEGF, VEGFR1, VEGFR2, sVEGFR1 and sVEGFR2 in bovine preselected follicles (PRF) and post‐selected follicles (POF). The mRNA expression of these five genes in both granulosa cells (GC) and theca cells (TC) was compared between follicles classified as PRF and POF based on their diameter and on their ratio of estradiol/progesterone (E2/P4). Results showed a lower expression of mRNA of sVEGFR1 and sVEGFR2 in POF than in PRF (p < .05). Regarding the mRNA expression of total VEGF, VEGFR1 and VEGFR2, there was no difference between POF and PRF follicles (p > .05). Our results showed that the mRNA expression of VEGFR2 and sVEGFR1 was more abundant than the expression of VEGFR1 and sVEGFR2, while GC was the main source of mRNA for total VEGF. On the other hand, TC was the follicular compartment where the receptors were most expressed. Our results suggest that non‐dominant follicles maintain a greater concentration of the mRNA expression of both membrane and soluble VEGF receptors. On the other hand, follicular dominance is related to a reduction in the mRNA expression of sVEGFR1 and sVEGFR2, which may favour VEGF binding with VEGFR2 and, hence, improve the follicular health and development.  相似文献   

Shape transition of germanium nanocrystals on a silicon (001) surface from pyramids to domes     

G Medeiros-Ribeiro  AM Bratkovski  TI Kamins  DAA Ohlberg  RS Williams 《Science (New York, N.Y.)》1998,279(5349):353-355

Chemical vapor deposition of germanium onto the silicon (001) surface at atmospheric pressure and 600 degrees Celsius has previously been shown to produce distinct families of smaller (up to 6 nanometers high) and larger (all approximately 15 nanometers high) nanocrystals. Under ultrahigh-vacuum conditions, physical vapor deposition at approximately the same substrate temperature and growth rate produced a similar bimodal size distribution. In situ scanning tunneling microscopy revealed that the smaller square-based pyramids transform abruptly during growth to significantly larger multifaceted domes, and that few structures with intermediate size and shape remain. Both nanocrystal shapes have size-dependent energy minima that result from the interplay between strain relaxation at the facets and stress concentration at the edges. A thermodynamic model similar to a phase transition accounts for this abrupt morphology change.  相似文献   

Self-oriented regular arrays of carbon nanotubes and their field emission properties   总被引：4，自引：0，他引：4  

S Fan  MG Chapline  NR Franklin  TW Tombler  AM Cassell  H Dai 《Science (New York, N.Y.)》1999,283(5401):512-514

The synthesis of massive arrays of monodispersed carbon nanotubes that are self-oriented on patterned porous silicon and plain silicon substrates is reported. The approach involves chemical vapor deposition, catalytic particle size control by substrate design, nanotube positioning by patterning, and nanotube self-assembly for orientation. The mechanisms of nanotube growth and self-orientation are elucidated. The well-ordered nanotubes can be used as electron field emission arrays. Scaling up of the synthesis process should be entirely compatible with the existing semiconductor processes, and should allow the development of nanotube devices integrated into silicon technology.  相似文献   

Hyperoxaluria,hyperglycoluria and renal oxalosis in Gilbert's potoroos (Potorous gilbertii)          下载免费PDF全文

D Forshaw  AM Horwitz  K Ellard  JA Friend  L Greed  M Metz 《Australian veterinary journal》2017,95(7):250-258

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