Prevalence of resistance in sheep nematode populations to benzimidazole and levamisole anthelmintics in the high rainfall areas of South Australia     

I. BEVERIDGE  NJS ELLIS  MJ RILEY  TH BROWN 《Australian veterinary journal》1990,67(11):413-415

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Low IgA concentration in the tears of German shepherd dogs   总被引：1，自引：0，他引：1  

MJ DAY 《Australian veterinary journal》1996,74(6):433-434

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Arthur Webster Pty Ltd have provided the following comment     

MJ LINDSEY 《Australian veterinary journal》1994,71(2):59-60

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Kinome profiling of Arabidopsis using arrays of kinase consensus substrates     

Tita Ritsema  Jos Joore  Wilbert van Workum  Corné MJ Pieterse 《Plant methods》2007,3(1):3-10

Background  

Kinome profiling aims at the parallel analysis of kinase activities in a cell. Novel developed arrays containing consensus substrates for kinases are used to assess those kinase activities. The arrays described in this paper were already used to determine kinase activities in mammalian systems, but since substrates from many organisms are present we decided to test these arrays for the determination of kinase activities in the model plant species Arabidopsis thaliana.  相似文献   

Quantification of Sexual Steroid Hormones in Faeces of Iberian Wolf (Canis lupus signatus): A Non‐invasive Sex Typing Method     

I Barja  G Silván  S Rosellini  A Piñeiro  MJ Illera  JC Illera 《Reproduction in domestic animals》2008,43(6):701-707

The determination of gender in wild animals is essential for behavioural and ecological studies, and also for conservation. The objectives of this study were (i) the determination of gender in faecal samples of Iberian wolf based on the differential concentrations of sexual steroid hormones (SSH) and (ii) to analyse the profiles of SSH in males and females (considering the gender determination carried out previously) during the non‐reproductive and reproductive periods. The quantification of androgens (testosterone, T), progestin (progesterone, P) and oestrogen (oestradiol, E) was conducted by means of enzyme immunoassay. The k‐means conglomerate analysis showed that the 59 faecal samples grouped into three different conglomerates, considering SSH levels. Groups 1 and 2 showed higher levels of T than group 3. Therefore, the faecal samples included in groups 1 and 2 (17 samples) corresponded to males and those of group 3 (42 samples) to females. The levels of T + P + E and T/P were higher in the group of males than in the group of females. The results of this study also showed that levels of T in males were higher during the reproductive period than in the non‐reproductive period. However, the concentrations of P and E turned out to be higher during the non‐reproductive season. In females, the levels of the three hormones (T, P and E) were higher during the reproductive period.  相似文献   

Metformin blocks mitochondrial membrane potential and inhibits sperm motility in fresh and refrigerated boar spermatozoa          下载免费PDF全文

D Martin‐Hidalgo  MJ Bragado 《Reproduction in domestic animals》2018,53(3):733-741

Metformin is clinically used to treat diabetes. Given its role‐impacting metabolism, metformin has been also added to semen cryopreservation media showing specie‐dependent effects. We aimed to investigate metformin effects in both fresh (38.5°C for 2, 24 hr) and refrigerated (17°C for 10 days) boar spermatozoa. Metformin (2 hr) does not affect fresh sperm viability, membrane lipid organization nor acrosome integrity. However, metformin (24 hr) blocks sperm ΔΨm and significantly reduces % motile spermatozoa (65%), % progressive spermatozoa (50%), % rapid (100%), velocities VCL (69%), VSL (86%), VAP (78%) and motility coefficients. Metformin‐including extender does not modify sperm viability, membrane lipid organization or acrosome integrity. Furthermore, it significantly reduces high ΔΨ‐population spermatozoa at refrigeration day 4. Metformin also significantly reduces sperm motility during refrigeration. Summarizing, metformin inhibits both boar sperm ΔΨ and motility in any sperm condition studied: fresh and refrigerated. These findings dissuade metformin as an additive to improve boar sperm quality.  相似文献   

Fish mortality due to acute ammonia exposure   总被引：2，自引：0，他引：2  

J. V. TARAZONA  MJ. MU&#;OZ  J. A. ORTIZ  MO. NUNÉZ  J. A. CAMARGO 《Aquaculture Research》1987,18(2):167-172

Abstract. Fish mortality in the River Umia, near Villagarcia de Arosa, Pontevedra, Spain, which occurred after a discharge from a food sewage works, is studied. Physico-chemical parameters of water samples from sewage and from the River Umia were studied and concentrations of ammonia sufficiently high to cause toxic effects in fish (702 mg/1 and 302 mg/1 as total ammonia) were found. The toxicity of two concentrations of ammonia was investigated on goldfish, Carassius auratus (L.), using water quality values the same as found in the River Umia, to obtain the same concentrations of un-ionized ammonia (2·13 mg/1 and 0·91 mg/1). Fish exposed to high concentrations died within 24 h and three of six, which were exposed to the lowest concentration died within 96h. All fish at first showed symptoms of hyper excitability and hyperventilation, and at times of rest showed a decrease in respiration. Post-mortem findings generally included gill congestion and haemorrhage.  相似文献   

Scanning Single-Electron Transistor Microscopy: Imaging Individual Charges     

MJ Yoo  TA Fulton  HF Hess  RL Willett  LN Dunkleberger  RJ Chichester  LN Pfeiffer  KW West 《Science (New York, N.Y.)》1997,276(5312):579-582

A single-electron transistor scanning electrometer (SETSE)-a scanned probe microscope capable of mapping static electric fields and charges with 100-nanometer spatial resolution and a charge sensitivity of a small fraction of an electron-has been developed. The active sensing element of the SETSE, a single-electron transistor fabricated at the end of a sharp glass tip, is scanned in close proximity across the sample surface. Images of the surface electric fields of a GaAs/AlxGa1-xAs heterostructure sample show individual photo-ionized charge sites and fluctuations in the dopant and surface-charge distribution on a length scale of 100 nanometers. The SETSE has been used to image and measure depleted regions, local capacitance, band bending, and contact potentials at submicrometer length scales on the surface of this semiconductor sample.  相似文献