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11.
Takuya HIROSE Takayuki OGURA Keisuke TANAKA Jun MINAGUCHI Takeshi YAMAUCHI Toshiyuki FUKADA Yoh-ichi KOYAMA Kazushige TAKEHANA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1385-1389
Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are
identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a
crucial role in formation of the extracellular matrix. It has been reported that the loss
of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro
dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1
signaling pathway is observed, although the relationship between the dermis abnormalities
and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the
dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS.
Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the
amount of collagen were decreased, and there were very few collagen fibrils with diameters
of more than 150 nm in Zip13-KO mice dermis. We also found that the
TGF-β1 level was significantly higher in Zip13-KO mice serum. These
results suggest that collagen synthesis and collagen fibril fusion might be impaired in
Zip13-KO mice and that the possible decrease of decorin level by
reduction of the DS ratio probably caused an increase of free TGF-β1 in
Zip13-KO mice. In conclusion, skin fragility due to defective
ZIP13 protein may be attributable to impaired extracellular matrix
synthesis accompanied by abnormal peripheral TGF-β homeostasis. 相似文献
12.
NAOTAKA OMOTO MAMORU MAEBAYASHI ERI MITSUHASHI KOJI YOSHITOMI SHINJI ADACHI KOHEI YAMAUCHI 《Fisheries Science》2002,68(5):1047-1054
The effects of giving oral estradiol-17β (E2 ) and 17α-methyltestosterone (MT) on gonadal sex differentiation in the F2 hybrid sturgeon, the bester ( Huso huso female × Acipenser ruthenus male), are investigated. Giving E2 at 10 μg/g diet to fish from 14 months until 31 months of age induced incomplete feminization and resulted in approximately 40% abnormal ovary development in which oocytes were observed without ovarian lamellar structures and gonadal shape was similar to normal testis. Giving MT at 25 μg/g diet for the same duration failed to induce masculinization, and resulted in approximately 30% undeveloped gonads even at 30–37 months of age. In contrast, E2 and MT at only 1 μg/g diet given from 3 to 18 months of age was sufficient to induce feminization and masculinization, respectively. In these fish, feminization and masculinization were observed at 9 months, when most putative ovaries and testes were histologically distinguishable by the shape of the gonadal surface. These results indicate that sex reversal can be induced in these fish by hormone treatment that is started at 3 months age, before morphological differentiation occurs on the stroma of the gonads. 相似文献
13.
以盒维数法分形分析水稻根系形态特征及初探其与锌吸收积累的关系 总被引：10，自引：0，他引：10
利用特定根盒装土, 培养4个水稻品种(MADHUKAR、IR8192-200、IR26、IR8192-31)植株, 用钉板法结合透明塑料膜固定获得近似原位根系样品, 扫描得到根系的二维平面图像, 以分形理论为基础, 利用盒维数法结合根系图像分形分析程序计算根系构型的分形维数和分形丰度, 比较各品种根系的形态特征, 并对分形参数、根系长度和 植株锌含量间的相关关系做了初步探讨。结果表明, 根系分形维数和分形丰度以MADHUKAR最大, IR8192-200最小, 说明MADHUKAR根系分支多, 在土壤中拓展体积大。分形维数、分形丰度与根系总长度之间均呈明显正相关, 而且根系总长度与分形丰度相关系数高于与分形维数的相关系数。分形维数和分形丰度与植株地上部干重、单位Zn浓度所产出的地上部生物量、地上部Zn吸收总量之间均呈显著正相关, 与地上部Zn浓度呈负相关。水稻根系形态和构型的变化影响植株生长, 影响植株Zn吸收积累及体内Zn的利用效率。盒维数法分形分析模型可用于研究水稻根系形态和构型, 为其提供新方法。 相似文献
14.
Kazuki YAMAGAMI Nobuhiko YAMAUCHI Kaiyu KUBOTA Sho NISHIMURA Vishwajit Sur CHOWDHURY Kenichi YAMANAKA Masashi TAKAHASHI Shoji TABATA Masa-aki HATTORI 《The Journal of reproduction and development》2014,60(6):468-475
The forkhead box a (Foxa) protein family has been found to play important roles in mammals. Recently, the expression of Foxa2 was reported in the mouse uterus, and it was reported to be involved in regulation of implantation. However, the regulation of Foxa2 expression in the uterus is still poorly understood. Therefore, the present study was conducted to investigate the expressional profiles of Foxa2 in the rat uterus during the estrus cycle and pregnancy. Furthermore, the effect of steroid hormones and Hedgehog protein on the expression of Foxa2 was analyzed in vivo and in vitro. In this study, the level of expression of Foxa2 was low in the rat uterus during the different stages of the estrus cycle. However, the expression increased transiently during early pregnancy at 3.5 days post coitus (dpc) and decreased at 5.5 dpc. In ovariectomized rats, P4 treatment had no effect on the
expression of Foxa2 compared with the expression in control animals. Moreover, the expression of Foxa2 in cultured epithelial cells was not increased by P4 treatment in vitro. However, Foxa2 expression was significantly decreased in the rat uterus after 24 h of E2 treatment. Treatment of cells with a recombinant Hedgehog protein significantly increased the expression of Foxa2. These results suggest that the expression of Foxa2 may transiently increase just before the implantation and it may be regulated by E2 and Hedgehog protein. 相似文献
15.
Koh-en YAMAUCHI 《Animal Science Journal》2007,78(4):356-370
The general histological features of the small intestine are well known. However, histological intestinal alterations induced by ingested feeds have not been established. To demonstrate such a relationship; (i) the post‐hatching development of intestinal villi in chickens was observed; (ii) histological intestinal differences were compared in White Leghorns and broilers; and (iii) histological intestinal alteration was investigated in fasting and refeeding treatments, using chickens and piglets. Histological intestinal recovery responses; (iv) to luminal nutrient absorption, intraluminal physical stimulation, or intravenous parenteral alimentation in fasted chickens, as well as; (v) to particle size and nutritional level of refed diets in fasted chickens and piglets were also compared. To induce compensatory enlargement of ileal villi; (vi) the jejunum was surgically resected. To reconfirm the histological intestinal alterations induced artificially; (vii) conventional feeding experiments were enforced in chickens and piglets. These fundamental investigations demonstrate that the high values of villus height, cell area and cell mitosis numbers as well as protuberated epithelial cells are hypertrophied by ingested feeds . Finally, as examples of practical use in the animal production field; (viii) useful feed resources to improve the growth performance by activating the intestinal function in chickens and piglets; and (ix) an effective forced molting method are introduced. Based on the histological intestinal alterations related with growth performance in chickens and piglets, this article reviews progress in the establishment of a histological intestinal index to assess the intestinal function. 相似文献
16.
Satoshi KAWAHARA Shin-ichi TAKENOYAMA Kazuaki TAKUMA Michio MUGURUMA Kiyoshi YAMAUCHI 《Animal Science Journal》2009,80(4):468-474
We investigated the effects of dietary conjugated linoleic acid (CLA) on fatty acid composition and lipid oxidation in breast meat of broiler chickens. Broiler chickens (28-day-old females) were fed diets containing experimental oils at 20 g/kg diet for 28 days. The experimental oils consisted of either a 2:0, 1:1, or a 0:2 (wt : wt) ratio of safflower oil (high linoleic acid content) to a commercial CLA mixture. In this study, dietary CLA supplementation significantly increased the composition and content of CLA in chicken meat. The predominant CLA in meat from birds with supplemented diets was the cis -9, trans -11 isomer. The proportion of saturated fatty acid in meat significantly increased with increasing CLA supplementation, with a corresponding decrease in monounsaturated fatty acid. Dietary CLA also reduced thiobarbituric acid reactive substances (TBARS) values in raw meat during storage at 4°C for 5 days. These results provide evidence that CLA feeding is a practical strategy not only for adding nutritional benefits to chicken meat but also for improving meat quality including oxidative stability. 相似文献
17.
Norihito YAMAUCHI Seizo HORIUCHI Mamoru SATOU 《Journal of General Plant Pathology》2001,67(4):288-290
Isolates of Fusarium oxysporum f. sp. lactucae obtained from six localities in Japan were divided into three patho-genicity groups. Group 1 was highly pathogenic to lettuce
cultivars of crisphead and red leaf types and was less pathogenic to butterhead and green leaf type cultivars. Group 2 was
highly pathogenic to butterhead type and less pathogenic to crisphead and leaf types. Group 3 was less pathogenic to all lettuce
types than groups 1 and 2. These results indicated pathogenic differentiation in F. oxysporum f. sp. lactucae, roughly relating to horticultural types of host lettuce cultivars.
Received 21 February 2001/ Accepted in revised form 28 May 2001 相似文献
18.
Akiyoshi EGASHIRA Nobuhiko YAMAUCHI Keiko TANAKA Chihiro MINE Hitomi OTSUBO Masao MURAKAMI Md. Rashedul ISLAM Misako OHTSUKA Naomi YOSHIOKA Takashi KURAMOTO 《The Journal of reproduction and development》2015,61(6):595-600
The presence of multinucleated blastomeres (MNBs) in embryos is associated with poor developmental
competence in assisted reproductive technologies. This phenomenon is observed not only in humans but also in
other animal species. The purpose of the present study was to investigate the characteristics of embryos with
MNBs (MNB embryos) that could be utilized in embryo transfer. The developmental rate of MNB embryos to the
blastocyst stage (50.8%) was significantly lower than that of normal embryos (73.3%) (P < 0.05). The
clinical pregnancy rates of fresh embryo transfer (ET) using day 2 or day 3 embryos were significantly lower
in MNB embryos (5.1%) compared with normal embryos (24.0%) (P < 0.05). In the case of frozen-thawed ET
using a single vitrified/warmed blastocyst, however, the clinical pregnancy rate of MNB embryos was close to
that of normal embryos (59.1% vs. 52.8%). Thus, the findings of the present study suggest
that the frozen-thawed ET of MNB embryos might improve the potential for implantation followed by successful
pregnancy. 相似文献
19.
20.
Lokaewmanee K Kanto U Juttupornpong S Yamauchi KE 《Tropical animal health and production》2011,43(2):377-381
Determinations of digestibility of dry matter (DM), digestible energy (DE), and metabolizable energy (ME) in cassava chips
with different levels of crude fiber (CF) were measured in growing pigs (20 kg) and finishing pigs (60 kg). The treatments
were (1) cassava starch (0% CF), (2) peeled cassava chips (2.5% CF), (3) non-peeled washed cassava chips (3.9% CF), and (4)
non-peeled and non-washed cassava chips (5.2% CF). In the growing pigs, peeled cassava chips, non-peeled washed cassava chips,
and non-peeled and non-washed cassava chips had DM digestibility of 87.51%, 78.63%, and 73.89%, respectively. Their DE was
3.69, 3.49, and 3.32 Mcal/kg DM, respectively (DE of cassava starch is 3.90 Mcal/kg DM). ME was 3.54, 3.35, and 3.19 Mcal/kg
DM, respectively (ME of cassava starch is 3.74 Mcal/kg DM). On the other hand, in the finishing pigs, the digestibility of
DM was 89.13%, 80.63%, and 76.13%, respectively. Their DE was 3.72, 3.53, and 3.43 Mcal/kg DM, respectively (DE of cassava
starch is 3.91 Mcal/kg DM). ME was 3.57, 3.38, and 3.29 Mcal/kg DM, respectively (ME of cassava starch is 3.75 Mcal/kg DM).
These values increased with decreasing CF content, and the peeled cassava chips had the highest values (P < 0.01). These suggest that the digestibility values of DM, DE, and ME of cassava chips is inversely related to the CF content
in cassava chips. It is recommended that cassava chips be peeled for better nutrition for growing and finishing pigs. 相似文献