A New and Simple Technique for the Isolation of Plasma Membrane Lipids from Root-Tips     

Tadao Wagatsuma  Matsuo Uemura  Wataru Mitsuhashi  Masayoshi Maeshima  Satoru Ishikawa  Takeshi Kawamura  Tetsuya Murayama  Yoshihito Shiono  Md. Shahadat Hossain Khan  Keitarou Tawaraya 《Soil Science and Plant Nutrition》2005,51(1):135-139

It has been suggested that plasma membrane (PM) lipids play a major role in aluminum (Al) tolerance; however, no direct investigations have been carried out using PM lipids from root-tips. Here we report a new technique for PM isolation as an alternative to the laborious two-polymer phase partitioning method that is commonly applied, as follows: 1) separation of protoplasts from 1-cm root-tip portions by enzymatic digestion, 2) attachment of the purified protoplasts to glass plates coated with polylysine, 3) preparation of PM ghosts by successive burst of the attached protoplasts using three separate buffer solutions (25 mM PIPES, 5 mM EDTA, and 2 mM MgCl₂, at pH 7.0) with slow stirring for 60 s. The PMs were confirmed to be devoid of organelle membranes by fluorescence microscopy, thin layer chromatography (TLC) and western blot analysis. The PM lipids obtained were found to be useful for studies on their differential permeability and lipid composition between lines of triticale or cultivars of maize under Al stress.  相似文献   

Functional annotation of a full-length Arabidopsis cDNA collection   总被引：2，自引：0，他引：2  

Seki M  Narusaka M  Kamiya A  Ishida J  Satou M  Sakurai T  Nakajima M  Enju A  Akiyama K  Oono Y  Muramatsu M  Hayashizaki Y  Kawai J  Carninci P  Itoh M  Ishii Y  Arakawa T  Shibata K  Shinagawa A  Shinozaki K 《Science (New York, N.Y.)》2002,296(5565):141-145

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Foot rot disease of sweet potato in Japan caused by Diaporthe destruens: first report,pathogenicity and taxonomy     

Maeda  Ai  Minoshima  Ayaka  Kawano  Shinji  Nakamura  Misa  Takushi  Tetsuya  Yamashiro  Maki  Kawamura  Futoshi  Oshiro  Atsushi  Ichinose  Katsuya  Okada  Yoshihiro  Rossman  Amy Y.  Hirooka  Yuuri 《Journal of General Plant Pathology》2022,88(1):33-40

Journal of General Plant Pathology - Foot rot, also known as stem rot or tuber-root rot syndrome, on sweet potato occurs in sweet potato fields in Okinawa, Japan. Isolates of one fungal species...  相似文献   

Management of nitrogen fertilizer application rates based on soil nitrogen fertility with the goal of lowering nitrate nitrogen concentrations in Sudangrass (Sorghum sudanense (Piper) Stapf)     

Yoshihito SUNAGA  Hisatomi HARADA  Tahei KAWACHI  Tetsuya HATANAKA 《Soil Science and Plant Nutrition》2008,54(4):543-554

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Gastrointestinal uptake of nasunin, acylated anthocyanin in eggplant   总被引：1，自引：0，他引：1  

Ichiyanagi T  Terahara N  Rahman MM  Konishi T 《Journal of agricultural and food chemistry》2006,54(15):5306-5312

We previously showed that nasunin, acylated anthocyanins in eggplant peel, comprises two isomers, cis-nasunin and trans-nasunin. In this study, gastrointestinal absorption of cis- and trans-nasunins was studied in rats. Orally administered nasunins were quickly absorbed in their original acylated forms and maximally appeared in blood plasma after 15 min. When the maximum plasma concentration and area under the plasma concentration curve were normalized by orally administered dose (micromoles per kilogram), there was no significant difference in the uptake efficiency between two isomers and both exhibited a plasma level almost identical to that of delphinidin 3-O-beta-D-glucopyranoside. However, metabolites such as 4'-O-methyl analogues and extended glucuronides which were observed for delphinidin 3-O-beta-D-glucopyranoside and cyanidin 3-O-beta-D-glucopyranoside metabolisms were not detected in urine or blood plasma. Moreover, deacylated and glycolytic products of nasunins such as delphinidin 3-O-beta-D-glucopyranoside or delphinidin (aglycone) were also not detected in blood plasma even after oral administration for 8 h. These results indicated that nasunins were absorbed in their original acylated forms and exhibit a bioavailability almost identical to that of nonacylated anthocyanins.  相似文献   

Neuronal pathway from the liver modulates energy expenditure and systemic insulin sensitivity     

Uno K  Katagiri H  Yamada T  Ishigaki Y  Ogihara T  Imai J  Hasegawa Y  Gao J  Kaneko K  Iwasaki H  Ishihara H  Sasano H  Inukai K  Mizuguchi H  Asano T  Shiota M  Nakazato M  Oka Y 《Science (New York, N.Y.)》2006,312(5780):1656-1659

Coordinated control of energy metabolism and glucose homeostasis requires communication between organs and tissues. We identified a neuronal pathway that participates in the cross talk between the liver and adipose tissue. By studying a mouse model, we showed that adenovirus-mediated expression of peroxisome proliferator-activated receptor (PPAR)-g2 in the liver induces acute hepatic steatosis while markedly decreasing peripheral adiposity. These changes were accompanied by increased energy expenditure and improved systemic insulin sensitivity. Hepatic vagotomy and selective afferent blockage of the hepatic vagus revealed that the effects on peripheral tissues involve the afferent vagal nerve. Furthermore, an antidiabetic thiazolidinedione, a PPARg agonist, enhanced this pathway. This neuronal pathway from the liver may function to protect against metabolic perturbation induced by excessive energy storage.  相似文献   

Effect of plant density on growth and yield of new soybean genotypes grown under early planting condition in southwestern Japan     

Naoki Matsuo  Tetsuya Yamada  Yoshitake Takada  Koichiro Fukami  Makita Hajika 《Plant Production Science》2018,21(1):16-25

Soybeans planted in early to mid-June (early) are less affected by rainfall during rainy season than those conventionally planted in early to mid-July in southwestern Japan. Also, narrow row cultivation is expected to increase soybean yield and save labor for inter-tillage and ridging. Field experiments were performed in 2014 and 2015 to test the effect of plant density (high, middle, and low) under early planting condition on growth, yield, and several agronomical traits of Sachiyutaka A1 and three new genotypes (Sakukei 155, Kanto 127 and Shikoku 15). Early planting was performed in mid- to late June, even though rainy season started in early June. Higher plant densities produced 13% greater yield than low plant density through an increase in biomass accumulation, especially at R5. Among yield components, only pods m^?2 was significantly and positively correlated with yield, indicating that an increase in pods m^?2 led to a greater yield with higher plant densities. The yields of Sachiyutaka A1 were relatively stable for two years, but the lodging resistance should be further improved. Shikoku 15 had greater yield potential and lodging resistance, but its resistance to damping-off disease should be improved. Sakukei 155 with medium plant density produced relatively high and stable yield with less lodging. Although the yield of Kanto 127 fluctuated between experimental years, this genotype showed higher yield potential in higher plant densities with less lodging in 2015. Thus, Sakukei 155 and Kanto 127 with high or medium density may be suitable for early planting in this region.  相似文献   

Functional insulin‐like factor 3 (INSL3) hormone‐receptor system in the testes and spermatozoa of domestic ruminants and its potential as a predictor of sire fertility          下载免费PDF全文

Ali M. Pitia  Kyoko Uchiyama  Hiroaki Sano  Masashi Kinukawa  Yoshiaki Minato  Hiroshi Sasada  Tetsuya Kohsaka 《Animal Science Journal》2017,88(4):678-690

Insulin‐like factor 3 (INSL3) is essential for fetal testis descent, and has been implicated in the testicular and sperm functions in adult males; however, similar functions in domestic ruminants remain largely unknown. This study investigated the functional INSL3 hormone‐receptor system in adult ruminant testes and spermatozoa, and explored its potential to diagnose the fertility of sires. Testes and spermatozoa were obtained from fertile bulls, rams and he‐goats, whereas subfertile testes and spermatozoa were obtained only from bulls. As expected, INSL3 was visualized in Leydig cells, while we clearly demonstrated that the functional receptor, relaxin family peptide receptor 2 (RXFP2), enabling INSL3 to bind was identified in testicular germ cells and in the sperm equatorial segment of bulls, rams and he‐goats. In comparison to fertile bulls, the percentage of INSL3‐ and RXFP2‐expressing cells and their expression levels per cell were significantly reduced in the testes of subfertile bulls. In addition, the population of INSL3‐binding spermatozoa was also significantly reduced in the semen of subfertile bulls. These results provide evidence for a functional INSL3 hormone‐receptor system operating in ruminant testes and spermatozoa, and its potential to predict subfertility in sires.  相似文献   

Effects of climate change on reduction of internal bond strength of particleboard subjected to various climatic conditions in Japan     

Hideaki Korai  Katsuhiro Nakao  Tetsuya Matsui  Ken Watanabe  Yasushi Ishigooka 《Journal of Wood Science》2017,63(3):253-262

The effects of climate change on the reduction of internal bond strength (IB) of particleboard subjected to various climatic conditions in Japan were predicted. The temperature increased throughout Japan with increasing greenhouse gas (GHG) emissions, so the climate deterioration index (climate index for predicting the IB reduction of particleboard; a high index indicates a large IB reduction) also increased to decrease IB. The number of low-temperature areas decreased because of climate change. The rupture of bonding points in particleboard caused by outdoor exposure led to a IB reduction, and biodeterioration accelerated this reduction. In high-temperature areas, particleboard was found to be prone to biodeterioration, and the IB significantly reduced. An area wherein there was a significant decrease in the IB spread along the Pacific Ocean side of western Japan and the Sea of Japan in central Japan in relation to increasing GHG emissions. Particleboards are difficult to use outdoors because of climate change.  相似文献   

Novel reovirus isolation from an Ostrich (Struthio camelus) in Japan     

Sakai K  Ueno Y  Ueda S  Yada K  Fukushi S  Saijo M  Kurane I  Mutoh K  Yoshioka K  Nakamura M  Takehara K  Morikawa S  Mizutani T 《Veterinary microbiology》2009,134(3-4):227-232

An orthoreovirus was isolated from an Ostrich (Struthio camelus) and rapidly identified as orthoreovirus by the rapid determination of viral RNA sequences (RDV) system and electron microscopy. Phylogenetic analysis of the sigma A protein indicated that the isolate belonged to avian species and was closely related to chicken orthoreovirus strain 138. The results of the present study indicated that an ostrich orthoreovirus is slight different from other chicken orthoreoviruses and provided evidence of diversity among avian orthoreoviruses. To our knowledge, this is the first genetic report of an orthoreovirus isolated from an ostrich.  相似文献