An evaluation of the duration of efficacy of emamectin benzoate in the control of Caligus curtus Müller infestations in Atlantic cod, Gadus morhua L     

Hamre LA  Lunestad BT  Hannisdal R  Samuelsen OB 《Journal of fish diseases》2011,34(6):453-457

The duration of efficacy of emamectin benzoate in the control of Caligus curtus infestations in Atlantic cod, Gadus morhua L., was studied following an administration of 50 μg kg^?1 for seven consecutive days. No lice were found on medicated fish when challenged 1 week (challenge 1) or 5 weeks (challenge 2) following termination of medication, whereas the mean abundance of lice among the unmedicated fish was 17.9 and 19.3 lice per fish in challenge 1 and 2, respectively. Muscle concentrations of 19.5 ± 8.2 ng g^?1 and 3.4 ± 0.9 ng g^?1, respectively, and skin concentrations of 23.1 ± 10.8 and 4.2 ± 1.0 ng g^?1, respectively, were found 27 and 55 days following the termination of medication. Tissue concentrations and the duration of efficacy indicate a dosing regime for emamectin in cod, similar to the regime used for Atlantic salmon, Salmo salar L.  相似文献   

Effects of dietary lipids on the fatty acid composition of triglycerides and phospholipids in tissues of white sturgeon   总被引：1，自引：0，他引：1  

R. XU  S.S.O. HUNG  J. B. GERMAN 《Aquaculture Nutrition》1996,2(2):101-109

Eight purified diets were fed to juvenile white sturgeon, Acipenser transmontanus Rick, for 9 weeks to investigate the effect of dietary lipids on the fatty acid composition of phospholipids and triglycerides from muscle, liver and brain. The diets contained 150 g kg^?1 of oils from canola, corn, cod liver, lard, linseed, soybean, safflower, or a control mixture (corn oil/cod liver oil/lard, 1:1:1, by wt). Dietary lipids significantly (P≤ 05) affected the composition of tissue triglycerides and phospholipids. Tissue triglyceride fatty acid composition ranged widely, in parallel with the dietary lipids, while phospholipids changes were more conservative. Brain phospholipid fatty acid composition was less responsive to diet compared with that in muscle and liver. Considerable amounts of n-6 and n-3 long chain polyun-saturated fatty acids (> C₂₀) were found in triglycerides and phospholipids with all diets, demonstrating that white sturgeon can desaturate and elongate linoleic acid (18:2n–6) and linolenic acid (18:3n–3). Further, the products of the Δ6 desaturase, i.e. 18:3 n–6 and 18:4n–3, were relatively abundant in triglyceride, suggesting that the Δ6 desaturase might not be a limiting step in the process in white sturgeon. Nevertheless, accumulation of both EPA and DHA was greater in the sturgeon fed fish oil than those fed linseed oil, indicating that muscle triglyceride EPA and DHA levels are best enhanced by diets rich in preformed EPA and DHA.  相似文献   

Direct actions of serotonin on gonadotropin-II and growth hormone release from goldfish pituitary cells: interactions with gonadotropin-releasing hormone and dopamine and further evaluation of serotonin receptor specificity     

A.O.L. Wong  C.K. Murphy  J.P. Chang  C.M. Neumann  A. Lo  R.E. Peter 《Fish physiology and biochemistry》1998,19(1):23-34

In this study, the direct actions of serotonin (5HT) on gonadotropin (GTH)-II and growth hormone (GH) release in the goldfish were tested at the pituitary cell level. 5HT (10 nM - 10 µM) stimulated GTH-II but inhibited GH release from perifused goldfish pituitary cells in a dose-dependent manner. The minimal effective dose of 5HT tested to suppress basal GH secretion (10 nM) was 10-fold lower than that to stimulate GTH-II release (100 nM). The GTH-II releasing effect of 5HT was abolished by repeated 5HT treatment (10 µM) whereas the corresponding inhibition on GH release was unaffected. These results suggest that 5HT receptors on goldfish gonadotrophs and somatotrophs exhibit intrinsic differences in terms of sensitivity to stimulation and resistance to desensitization. Salmon GTH-releasing hormone (sGnRH, 100 nM) stimulated GTH-II and GH release from goldfish pituitary cells. The GTH-II releasing action of sGnRH was unaffected by simultaneous treatment of 5HT (1 µM). However, the corresponding GH response to sGnRH (100 nM) was inhibited. In the goldfish, dopamine is known to stimulate GH release through activation of pituitary D1 receptors. In the present study, the GH-releasing action of dopamine (1 µM) and the D1 agonist SKF38393 (1 µM) was significantly reduced by 5HT (1 µM). To examine the receptor specificity of 5HT action, the effects of 5HT1 and 5HT2 analogs on GTH-II and GH release were tested in goldfish pituitary cells. The 5HT1 agonist 8OH DPAT (0.1 and 1µM) and 5HT2 agonist methyl 5HT (0.1 1µM) mimicked the GTH-II releasing effect of 5HT. The 5HT1 agonist 8OH DPAT (0.1 and 1µM) also stimulated GH release but the 5HT2 agonist methyl 5HT (0.1 and 1µM) was inhibitory to basal GH secretion. In addition, 5HT (1µM) -stimulated GTH-II release was abolished by the 5HT1 antagonist methiothepin (10µM) and 5HT2 antagonist mianserin (10µM). Similarly, the inhibitory action of 5HT (1µM) on basal GH release was blocked by the 5HT2 antagonist mianserin (10µM). The 5HT1 antagonist methiothepin (10µM) was not effective in this regard. These results, taken together, indicate that 5HT exerts its regulatory actions on GTH-II and GH release in the goldfish directly at the pituitary cell level, probably through interactions with other regulators including sGnRH and dopamine. The GTH-II releasing action of 5HT is mediated through 5HT2 and possibly 5HT1 receptors. The inhibition of 5HT on basal GH release is mediated through 5HT2 receptors only. Apparently, 5HT1 receptors are not involved in this inhibitory action. In this study, a paradoxical stimulatory component of 5HT on GH release by activating 5HT1 receptors is also implicated.  相似文献   

Attenuation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced gap junctional intercellular communication (GJIC) inhibition in MCF-10A cells by c9,t11-conjugated linoleic acid     

Rakib MA  Kim YS  Jang WJ  Choi BD  Kim JO  Kong IK  Ha YL 《Journal of agricultural and food chemistry》2010,58(22):12022-12030

The protective effect of c9,t11-conjugated linoleic acid (CLA) on the inhibition of gap junctional intercellular communication (GJIC) was examined in a human mammary epithelial cell line (MCF-10A) treated with 12-O-tetradecanoylphorbol-13-acetate (TPA), relative to t10,c12-CLA isomer. TPA inhibited GJIC in a dose-dependent and reversible manner and was associated with connexin 43 phosphorylation. Pretreatment of 20 μM c9,t11-CLA for 24 h prior to 60 nM TPA for 1 h prevented the inhibition of GJIC by reducing the phosphorylation of connexin 43 via suppressing extracellular signal-regulated kinases (ERK1/2) activation. Reactive oxygen species (ROS) accumulation by TPA was attenuated by c9,t11-CLA. The efficacy of c9,t11-CLA in protecting inhibition of GJIC, connexin 43 phosphorylation, and ROS production was superior to that of t10,c12-CLA. These results suggest that c9,t11-CLA, including t10,c12-CLA, prevents the carcinogenesis of MCF-10A cells by protecting down-regulation of GJIC during the cancer promotion stage, and lack of their toxicities could be an excellent indicator for the chemoprevention of breast cancer.  相似文献   

Isotopic evidence and quantification assessment of in situ RDX biodegradation in the deep unsaturated zone     

S. Sagi-Ben Moshe  O. Dahan  N. Weisbrod  E. Adar 《Soil biology & biochemistry》2010,42(8):1253-1262

Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is an explosive compound whose extensive use has resulted in significant contamination of soils and groundwater worldwide. We studied its in situ biodegradation along the unsaturated zone beneath an explosives wastewater lagoon using compound-specific isotope analysis (CSIA) of RDX in the unsaturated zone, together with biodegradation slurry experiments under anaerobic conditions. We found the highest degradation potential of RDX and its nitroso derivatives in the upper part of the soil profile while in the lower parts, RDX-degradation potential was lower and the nitroso derivatives tended to persist. This was also observed in the field, as reflected by the isotopic composition of RDX along the profile. We also found a correlation between biodegradation potential and clay content: biodegradation was further enhanced in layers characterized by high-clay content or in those influenced by the high-clay layers. In addition, in the presence of high organic matter content, further enhancement of biodegradation was observed. We obtained different isotopic enrichment factors (?) for RDX biodegradation in different sections of the unsaturated profile and suggest that different degradation pathways exist simultaneously in situ, in variable proportions. Using the range of enrichment factors, we were able to assess the biodegradation extent of RDX at different sampling points along the profile, which ranged between 30 and 99.4%. The novel application of CSIA together with slurry experiments provides better insight into degradation processes that are otherwise difficult to detect and assess.  相似文献   

Evaluating grassed waterway efficiency in southeastern Iowa using WEPP     

D. Dermisis  O. Abaci  A. N. Papanicolaou  C. G. Wilson 《Soil Use and Management》2010,26(2):183-192

Properly calibrated, single storm event, model simulations can be valuable quantitative tools for evaluating the effectiveness of grassed waterways (GWWs) and identifying a threshold length for an effective GWW. Current specifications for GWW lengths in Iowa are lacking. The key objective of this study is to employ the well-established Water Erosion Prediction Project model (WEPP) for determining an effective (or threshold) GWW length for reducing runoff and sediment yields under a wide range of hydrologic and management conditions in an agricultural Iowa watershed, namely Clear Creek, IA. The advantage of this physically based, distributed-parameter model is its ability to replicate processes at the hillslope scale where GWW performance is mostly evaluated. Overall, 84 WEPP runs were performed for assessing (i) the effect that GWW length has on reducing runoff and sediment yields within a representative test hillslope, and (ii) the effect of the gradient of the drainage area on GWW efficiency. Results show that the GWW efficiency for all GWW lengths is governed by hydrology, expressed as Q_peak. The results suggest that the threshold length for an effective GWW is 500 m for the hydrologic conditions in this study and for a representative drainage area of approximately 27 ha. For all storm events, a threshold drainage area gradient of approximately 3% was found above which the GWW efficiency was independent of topographic steepness. The results demonstrate similar trends to other studies although the relative increases in reduction in runoff and sediment delivery differ between sites and are very much dictated by Q_peak and hydrologic soil group. The effectiveness of models to evaluate GWW efficiency for nearly saturated conditions and shallow flows is discussed.  相似文献   

International symposium on apiculture     

O F Grobov 《Veterinariia》1974,(12):107-108

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Nitrous oxide emission from soils at low moisture contents     

J.R. Freney  O.T. Denmead  J.R. Simpson 《Soil biology & biochemistry》1979,11(2):167-173

The production of nitrous oxide by soils was studied over short periods at a range of moisture contents up to field capacity with a highly-sensitive gas Chromatographic method.Nitrous oxide (N₂O) was emitted from all soils studied at all soil moisture contents, which ranged from air dry to field capacity. The rate of emission increased with increasing moisture content and with increasing temperature up to 37°C.The evolution of N₂O was not due to displacement of soil air during wetting. It was inhibited by HgCl₂ and toluene, and was prevented by formaldehyde and autoclaving. Thus it appeared to be due to microbiological processes.The results of experiments with nitrification and denitrification inhibitors suggest that a considerable part of the N₂O was produced by the oxidation of ammonia. Production by denitrification of nitrate cannot be ruled out. The relative importance of these two mechanisms probably depends on the moisture and oxygen content of the soil.It is concluded that the microbial production of N₂O is continuous in soil at all moisture contents. The process at low moisture contents constitutes an important component in the cycle which maintains the N₂O concentration in the atmosphere.  相似文献   

Effects of Sowing Methods on Upland Rice in Lowland Rice-Vegetable Sequence in Inland Valley     

S. O. Adigbo  A. O. Ojerinde  O. Ajayi  F. E. Nwilene 《农业科学与技术》2010,(3):1-10

Triple cropping in the inland valleys of southwestern Nigeria is technically possible but the third crop between the main crop and vegetables has poor establishment due to soil saturation. Identification of appropriate sowing methods is required in this niche. A field experiment was conducted at the University of Agriculture, Abeokuta, Nigeria in 2007/2008 and 2008/2009 cropping seasons to evaluate the effect of sowing methods on the performance of upland rice （Oryza spp.） in a lowland rice-vegetable sequence. The first, second, and third crops in the sequence were planted in May, September and December, respectively. Two upland rice varieties, namely New Rice for Africa 1 （NERICA 1） and ITA 150 constituted the main plot treatments while the subplot treatments were sowing methods. The mean grain yield of lowland rice for two years was 7.74 t ha^-1. The fluted pumpkin had a fresh leaf weight of 14.78 t ha^-1. The grain yields of the two upland rice varieties （1.81 and 1.85 t ha^-1） and sowing methods which ranged between 1.63 and 2.05 t ha^-1 were similar in both cropping seasons. However, the interaction of variety by sowing methods had a significant effect on grain yield. Transplanting and pre-germinated broadcast had the highest grain yield for NERICA 1 while dry broad cast and pre-germinated dibbled had the highest in ITA 150. Hence, the productivity of triple cropping in the inland valley was enhanced by sowing methods.  相似文献   

CORRESPONDENCE     

T. E. O. ANDREWS  C. C. STAPLETON 《Southern Forests》2013,75(1):94-95

This article reports on the research development of laminated pine telephone cross-arms. Twenty-one years of service testing under extreme conditions in South-West Africa and 19 years at Magaliesburg in the Transvaal proved such cross-arms to be a success. This success has led to further development In South Africa in fields such as laminated pine railway sleepers.  相似文献