Differentiating between analytical and diagnostic performance evaluation with a focus on the method comparison study and identification of bias     

Bente Flatland  Kristen R. Friedrichs  Stefanie Klenner 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2014,43(4):475-486

Prior to introduction of a new method to the diagnostic laboratory, analytical performance must be validated to ensure operation within the manufacturer's specifications and/or within predetermined quality requirements. In addition, the new method may require diagnostic performance assessment to ensure it differentiates between diseased and nondiseased individuals as intended. These 2 phases of assessment, while complementary, are not equivalent and require a different set of experiments, statistical analyses, and interpretation. Studies of analytical performance typically include a method comparison experiment, the purpose of which is to identify bias (inaccuracy) of the “test” (or “index”) method (new method) relative to a “comparative method” (established method). Analysis of method comparison data is facilitated by commercial software programs that present the statistical significance of identified bias; however, the clinical relevance of any bias also should be considered. Studies of diagnostic performance should not be pursued until analytical performance is fully characterized and may not be required for well‐established tests or for those for which results are nonspecific (ie, not referable to a specific disease or condition). Diagnostic performance assessment may include assessment of sensitivity, specificity, predictive values, odds ratios, and/or likelihood ratios. The purpose of this review is to clarify differences between the assessment of analytical and diagnostic performance, and to explore the method comparison study and bias assessment from a perspective not addressed in prior veterinary articles.  相似文献   

On a razor's edge: Status and prospects of the critically endangered Bellinger River snapping turtle,Myuchelys georgesi     

Bruce C. Chessman  Gerry McGilvray  Shane Ruming  Hugh A. Jones  Kristen Petrov  Darren P. Fielder  Ricky‐John Spencer  Arthur Georges 《水产资源保护:海洋与淡水生态系统》2020,30(3):586-600

1. In the summer and autumn of 2015, the Bellinger River snapping turtle (Myuchelys georgesi), a narrow‐range endemic of eastern New South Wales, Australia, suffered mass mortality from epidemic disease, apparently caused by a previously unknown virus. Information on the current population size and structure of M. georgesi, and the body condition and growth of the surviving individuals, is needed to support planning of conservation actions. Population estimates are also needed for a sympatric population of the widely distributed Macquarie turtle (Emydura macquarii), which has probably been introduced to the Bellinger River and may threaten the persistence of M. georgesi through hybridization, competition, and disease transmission.
2. Data from five turtle surveys between November 2015 and November 2018 were used to estimate populations of the two species in the Bellinger River by an analysis based on habitat extent and turtle detectability. Changes in the body condition of M. georgesi and the body growth of both species were also assessed.
3. Current populations of ~150 M. georgesi and ~500 E. macquarii are indicated, although the uncertainty of these estimates is high. The estimate for M. georgesi represents a decline of >90% from the historical population. Moreover, about 88% of the surviving M. georgesi are immature, and only about 5% are mature females. However, the body condition of the survivors has improved recently. Growth models suggest that M. georgesi matures later than E. macquarii, which may provide the latter with a competitive advantage.
4. Evidence presented here does not support a previous hypothesis that M. georgesi were predisposed to disease through malnutrition and consequently reduced immune competence caused by high water temperatures and low river flows. Continuing disease, hybridization, and interspecific competition are probably the greatest threats to the persistence of the species.

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Rare structural variants disrupt multiple genes in neurodevelopmental pathways in schizophrenia     

Walsh T  McClellan JM  McCarthy SE  Addington AM  Pierce SB  Cooper GM  Nord AS  Kusenda M  Malhotra D  Bhandari A  Stray SM  Rippey CF  Roccanova P  Makarov V  Lakshmi B  Findling RL  Sikich L  Stromberg T  Merriman B  Gogtay N  Butler P  Eckstrand K  Noory L  Gochman P  Long R  Chen Z  Davis S  Baker C  Eichler EE  Meltzer PS  Nelson SF  Singleton AB  Lee MK  Rapoport JL  King MC  Sebat J 《Science (New York, N.Y.)》2008,320(5875):539-543

Schizophrenia is a devastating neurodevelopmental disorder whose genetic influences remain elusive. We hypothesize that individually rare structural variants contribute to the illness. Microdeletions and microduplications >100 kilobases were identified by microarray comparative genomic hybridization of genomic DNA from 150 individuals with schizophrenia and 268 ancestry-matched controls. All variants were validated by high-resolution platforms. Novel deletions and duplications of genes were present in 5% of controls versus 15% of cases and 20% of young-onset cases, both highly significant differences. The association was independently replicated in patients with childhood-onset schizophrenia as compared with their parents. Mutations in cases disrupted genes disproportionately from signaling networks controlling neurodevelopment, including neuregulin and glutamate pathways. These results suggest that multiple, individually rare mutations altering genes in neurodevelopmental pathways contribute to schizophrenia.  相似文献   

Sorption-desorption of imidacloprid and its metabolites in soil and vadose zone materials     

Papiernik SK  Koskinen WC  Cox L  Rice PJ  Clay SA  Werdin-Pfisterer NR  Norberg KA 《Journal of agricultural and food chemistry》2006,54(21):8163-8170

Sorption-desorption is one of the most important processes affecting the leaching of pesticides through soil because it controls the amount of pesticide available for transport. Subsurface soil properties can significantly affect pesticide transport and the potential for groundwater contamination. This research characterized the sorption-desorption of imidacloprid (1-[(6-chloro-3-pyridinyl)-methyl]-N-nitro-2-imidazolidinimine) and three of its metabolites, 1-[(6-chloro-3-pyridinyl)methyl]-2-imidazolidinone (imidacloprid-urea), 1-[(6-chloro-3-pyridinyl)methyl]-4,5-dihydro-1H-imidazol-2-amine (imidacloprid-guanidine), and 1-[(6-chloro-3-pyridinyl)methyl]-1H-imidazol-2-amine (imidacloprid-guanidine-olefin), as a function of changing soil properties with depth in two profiles extending from the surface to a depth of 1.8 or 8 m. Sorption of each compound was highly variable and hysteretic in all cases. Normalizing the sorption coefficients (K(f)) to the organic carbon or the clay content of the soil did not reduce the variability in sorption coefficients for any compound. These results illustrate the importance of evaluation of the sorption data used to predict potential mobility. Understanding the variability of soil properties and processes as a function of depth is necessary for accurate prediction of pesticide dissipation.  相似文献   

Ophthalmic diagnostic testing and examination findings in a colony of captive brown pelicans (Pelecanus occidentalis)          下载免费PDF全文

Kristen M. O'Connell  Tammy M. Michau  Jessica M. Stine  Andrea T. Reid 《Veterinary ophthalmology》2017,20(3):196-204

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Monitoring Victoria’s public forests: implementation of the Victorian Forest Monitoring Program     

Andrew Haywood  Kristen Thrum  Andrew Mellor  Christine Stone 《Southern Forests》2018,80(2):185-194

A new strategic inventory across all publicly managed forests has been installed in Victoria. For the preceding two decades, Victoria’s forest inventory has been compiled from various data sources from across the state, an approach that doesn’t allow information about the nature and rate of changes to the resource to be estimated, or for projections or forecasts to be made. To address these limitations, the Victorian Forest Monitoring Program (VFMP) was established, to monitor Victoria’s progress in meeting a commitment towards sustainable forest management, and to satisfy requirements for state and national reporting. The purpose of the new program is to ‘assess and monitor the extent, state and sustainable development of Victoria’s public forests in a timely and accurate manner.’ The VFMP consists of a plot-based system of permanent observational units located on a state-wide grid. A combination of ground-based and aerial photo plots and remotely sensed data are used to capture a set of basic attributes that are used to derive indicators of sustainability. To meet the monitoring needs a remeasurement framework has been developed to guide the change estimation procedures. The program was designed and implemented between 2009 and 2015 and used to generate data and information for the most recent Victoria’s State of the Forest Report. The first annual change estimate is due to be completed in 2017. A summary of implementation and proposed maintenance is presented and discussed in this paper.  相似文献   

Effects of Cover Crops on Soil Quality: Selected Chemical and Biological Parameters     

Chathuri S Weerasekara  Ranjith P Udawatta  Clark J Gantzer  Shibu Jose  Kristen S Veum 《Communications in Soil Science and Plant Analysis》2017,48(17):2074-2082

Cover crops improve soil quality properties and thus land productivity. We compared soil chemical and biological changes influenced by hairy vetch (Vicia villosa Roth.) and cereal rye (Secale cereal) cover crops grown in Menfro silt loam (fine-silty, mixed, superactive, mesic Typic Hapludalfs), Mexico silt loam (fine, smectitic, mesic Vertic Epiaqualfs), or sand in the greenhouse. Cover crop biomass, soil β-glucosidase, β-glucosaminidase, and fluorescein diacetate (FDA) hydrolase activities, and soil chemical properties were measured at six, nine, and twelve weeks after planting. Cover crop biomass increased with highest (p < 0.0001) yields for hairy vetch and cereal rye in Menfro and Mexico soils, respectively. β-glucosaminidase, FDA, organic carbon (C), total nitrogen (N), and total phosphorus (P) contents significantly decreased in all soils for both cover crops. However, β-glucosidase activity significantly increased (p < 0.0001). Long-term field studies are needed to evaluate soil quality improvement under cover crops, especially for soils with marginal organic matter and fertility.  相似文献   

Pharmacokinetics and bioavailability of ketoprofen when compounded with iron dextran for use in nursing piglets     

Kristen J. Reynolds  Ron Johnson  Robert M. Friendship  Jennifer Brown  Saad Enouri  Ronette Gehring  Terri L. O&#x;Sullivan 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(11):1211

In Canada, piglets receive analgesia to control pain after surgical castration. There is interest in examining the potential to mix non-steroidal anti-inflammatory drugs with iron dextran prior to injection to minimize piglet handling and labor. The objective of this study was to compare pharmacokinetics and the relative bioavailability of ketoprofen given alone (3.0 mg/kg IM) versus the same dose of ketoprofen mixed with iron dextran (52.8 mg/kg IM) (ketoprofen + iron dextran) before injection in piglets. Piglets 8 to 11 d old were allocated into 2 treatment groups (n = 8/group). Plasma drug concentrations were measured using mass spectrometry at 13 time points after injection. No significant differences were detected between the 2 groups when examining pharmacokinetic parameters (e.g., C_max, T_max, AUC) or relative bioavailability for either S- or R-ketoprofen enantiomers (P > 0.05). However, pain control efficacy and food safety studies of these formulations are required to further examine this practice.  相似文献   

Maize lethal necrosis viruses and other maize viruses in Rwanda     

Theodore Asiimwe  Lucy R. Stewart  Kristen Willie  Deogracious P. Massawe  Jovia Kamatenesi  Margaret G. Redinbaugh 《Plant pathology》2020,69(3):585-597

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Salish Sea Chinook salmon exhibit weaker coherence in early marine survival trends than coastal populations          下载免费PDF全文

Casey P. Ruff  Joseph H. Anderson  Iris M. Kemp  Neala W. Kendall  Peter A. Mchugh  Antonio Velez‐Espino  Correigh M. Greene  Marc Trudel  Carrie A. Holt  Kristen E. Ryding  Kit Rawson 《Fisheries Oceanography》2017,26(6):625-637

Identifying factors that influence anadromous Pacific salmon (Oncorhynchus spp.) population dynamics is complicated by their diverse life histories and large geographic range. Over the last several decades, Chinook salmon (O. tshawytscha) populations from coastal areas and the Salish Sea have exhibited substantial variability in abundance. In some cases, populations within the Salish Sea have experienced persistent declines that have not rebounded. We analyzed a time series of early marine survival from 36 hatchery Chinook salmon populations spanning ocean entry years 1980–2008 to quantify spatial and temporal coherence in survival. Overall, we observed higher inter‐population variability in survival for Salish Sea populations than non‐Salish Sea populations. Annual survival patterns of Salish Sea populations covaried over smaller spatial scales and exhibited less synchrony among proximate populations relative to non‐Salish Sea populations. These results were supported by multivariate autoregressive state space (MARSS) models which predominantly identified region‐scale differences in survival trends between northern coastal, southern coastal, Strait of Georgia, and Puget Sound population groupings. Furthermore, Dynamic Factor Analysis (DFA) of regional survival trends showed that survival of southern coastal populations was associated with the North Pacific Gyre Oscillation, a large‐scale ocean circulation pattern, whereas survival of Salish Sea populations was not. In summary, this study demonstrates that survival patterns in Chinook salmon are likely determined by a complex hierarchy of processes operating across a broad range in spatial and temporal scales, presenting challenges to the management of mixed‐stock fisheries.  相似文献