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Elliott R. Jacobson Kristin H. Berry Dennis E. Brooks John F. Roberts 《Veterinary ophthalmology》2023,26(4):361-366
A wild Agassiz's desert tortoise, Gopherus agassizii, with bilateral eyelid reduction and plaques of tissue covering the superior surface of both corneas was examined in the field and subsequently submitted to the University of Florida for diagnostics. Polymerase chain reaction (PCR), from a swab of both corneas, was positive for Mycoplasma agassizii. Two months later, the tortoise was euthanatized and necropsied. There was increased bulbar exposure associated with dermal excoriation of periocular scales in both superior and inferior palpebra resulting in an increased palpebral fissure opening. Concurrently, there was bilateral conjunctivitis of the nictitating membranes and squamous metaplasia of the bulbar conjunctiva. Using PCR, Mycoplasma testudineum, another pathogen of tortoises, was identified in both nasal cavities, and the upper respiratory tract histopathological findings were consistent with those described for M. testudineum in Agassiz's desert tortoises. Although eye disease has been reported in desert and gopher (Gopherus polyphemus) tortoises with mycoplasmosis, widespread loss of palpebral tissue, conjunctivitis of the nictitans, and squamous metaplasia of the bulbar conjunctiva have not been reported in tortoises. 相似文献
63.
Joshua W. Beniston Rattan Lal Kristin L. Mercer 《Land Degradation \u0026amp; Development》2016,27(4):996-1006
Following the decline of industrial manufacturing, many US cities have experienced severe population reductions that have resulted in large areas of vacant land. Urban agriculture has emerged as a desirable land use for these spaces, but degraded soils are common. Therefore, we measured soil and plant responses to amendments and management in urban lots where vacant houses had recently been demolished in Youngstown, OH, USA. Soil degradation was observed following demolition activities in the form of compaction (bulk density of 1·5–1·8 Mg m−3) and low soil microbial biomass C (21 mg C kg−1 soil). Our split‐plot experiment measured the effects of organic matter (OM) amendments produced from yard wastes and the use of raised beds on soil properties and vegetable crop yields. Two years after their application, OM amendments resulted in significant improvement to a number of soil physical, chemical, and biological properties. Vegetable crop yields were improved by OM amendments in 2011 and by both OM amendments and the use of raised beds in 2012. A soil quality index, developed using factor analysis and the Soil Management Assessment Framework, produced values ranging from 0·60 to 0·85, which are comparable to those reported for rural agricultural soils. All results indicate that urban agriculture can be productive in vacant urban land and that amendments produced from urban yard wastes can improve soil quality at previously degraded sites and increase crop yields for urban agriculture. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
64.
Trigeminal ganglion (TG) neurons are important target cells for many alphaherpesviruses and constitute a major site of virus latency and reactivation. Earlier we showed that porcine TG neurons are remarkably more resistant towards (apoptotic) cell death resulting from infection by the swine alphaherpesvirus pseudorabies virus (PRV) compared to a broad range of other primary porcine cell types and that this resistance does not depend on the strongly anti-apoptotic US3 viral protein kinase (Geenen, K., Favoreel, H.W., Nauwynck, H.J., 2005a. Higher resistance of porcine trigeminal ganglion neurons towards pseudorabies virus-induced cell death compared with other porcine cell types in vitro. J. Gen. Virol. 86, 1251-1260). Although other viral anti-apoptotic proteins may be involved in survival of TG neurons during PRV infection, an additional factor may be that TG neurons possess a cell type-dependent capacity to withstand apoptosis compared to other cell types. To investigate this, we treated uninfected porcine TG cultures, swine kidney cells, and porcine superior cervical ganglion (SCG) neurons with several apoptosis-inducing reagents (staurosporine, camptothecin and genistein). None of these reagents were able to trigger substantial apoptotic cell death in TG neurons, whereas non-neuronal TG cells, swine kidney cells, and SCG neurons showed a clear dose-dependent increase in apoptosis using either of these reagents. In conclusion, sensory TG neurons may contain a cell type-specific capacity to withstand different apoptotic assaults, including infection with an alphaherpesvirus. 相似文献
65.
Herbert A Gerry NP McQueen MB Heid IM Pfeufer A Illig T Wichmann HE Meitinger T Hunter D Hu FB Colditz G Hinney A Hebebrand J Koberwitz K Zhu X Cooper R Ardlie K Lyon H Hirschhorn JN Laird NM Lenburg ME Lange C Christman MF 《Science (New York, N.Y.)》2006,312(5771):279-283
Obesity is a heritable trait and a risk factor for many common diseases such as type 2 diabetes, heart disease, and hypertension. We used a dense whole-genome scan of DNA samples from the Framingham Heart Study participants to identify a common genetic variant near the INSIG2 gene associated with obesity. We have replicated the finding in four separate samples composed of individuals of Western European ancestry, African Americans, and children. The obesity-predisposing genotype is present in 10% of individuals. Our study suggests that common genetic polymorphisms are important determinants of obesity. 相似文献
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Schafgans KE Armstrong PJ Kramek B Ober CP 《Journal of Feline Medicine and Surgery》2012,14(10):759-763
A 7-month-old female spayed domestic shorthair cat was presented for investigation of stertor, open mouth breathing without apparent distress, and chronic bilateral nasal discharge that was unresponsive to antibiotics. Complete bilateral bony choanal atresia was diagnosed with computed tomography and nasopharyngoscopy. Choanal atresia is an uncommon congenital condition where the choana (nasal passage into the nasopharynx) is blocked by abnormal bone or soft tissue uni- or bilaterally. The cat's clinical signs improved dramatically immediately after trans-palatal surgical correction. Post-surgical complications included the development of nasopharyngeal scar tissue and subsequent stenosis, persistent right-sided nasal discharge, and permanent damage to the right eye (blindness and cataract formation). Nasopharyngeal stenosis was managed with repeated balloon dilatations and temporary stenting, and the owner reported an excellent quality of life at 8-month follow-up. Bilateral bony choanal atresia has not been previously reported in cats. Uni- or bilateral choanal atresia should be considered in young cats presenting with refractory stertor, chronic nasal discharge, and/or open mouth breathing. 相似文献
68.
ObjectiveTo describe the pharmacodynamics and pharmacokinetics following an intravenous (IV) bolus dose of medetomidine in the horse.Study designProspective experimental trial.AnimalsEight, mature healthy horses age 11.7 ± 4.6 (mean ± SD) years, weighing 557 ± 54 kg.MethodsMedetomidine (10 μg kg?1) was administered IV. Blood was sampled at fixed time points from before drug administration to 48 hours post administration. Behavioral, physiological and biochemical data were obtained at predetermined time points from 0 minutes to 24 hours post administration. An algometer was also used to measure threshold responses to noxious stimuli. Medetomidine concentrations were determined by liquid chromatography-Mass Spectrometry and used for calculation of pharmacokinetic parameters using noncompartmental and compartmental analysis.ResultsPharmacokinetic analysis estimated that medetomidine peaked (8.86 ± 3.87 ng mL?1) at 6.4 ± 2.7 minutes following administration and was last detected at 165 ± 77 minutes post administration. Medetomidine had a clearance of 39.6 ± 14.6 mL kg?1 minute?1 and a volume of distribution of 1854 ± 565 mL kg?1. The elimination half-life was 29.1 ± 12.5 minutes. Glucose concentration reached a maximum of 176 ± 46 mg dL?1 approximately 1 hour post administration. Decreased heart rate, respiratory rate, borborygmi, packed cell volume, and total protein concentration were observed following administration. Horses lowered their heads from 107 ± 12 to 20 ± 10 cm within 10 minutes of drug administration and gradually returned to normal. Horse mobility decreased after drug administration. An increased mechanical threshold was present from 10 to 45 minutes and horses were less responsive to sound.Conclusion and clinical relevance Behavioral and physiological effects following intravenous administration positively correlate with pharmacokinetic profiles from plasma medetomidine concentrations. Glucose concentration gradually transiently increased following medetomidine administration. The analgesic effect of the drug appeared to have a very short duration. 相似文献
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70.
Two studies were conducted on a third-story rooftop to quantify the effect of solar radiation (full sun versus full shade) on several US native and non-native species for potential use on extensive green roofs. In the first study, plugs of six native and three non-native species were planted in May 2005 on substrates of two different depths (8.0 and 12.0 cm) both in sun and shade. Absolute cover (AC) was recorded using a point-frame transect during the growing season beginning in June 2005 and every 2 weeks thereafter for a period of 4 years. By week 174 (23 September 2008), most species exhibited different AC within a depth between sun and shade. However, when all species were combined, overall AC did not differ between sun and shade within a depth. This indicated that while species make-up was changing among solar radiation levels, that overall coverage was not significantly different between sun and shade. For all substrate depths and solar levels, the most abundant species were Sedum acre, Allium cernuum, Sedum album ‘Coral Carpet’, and Talinum calycinum. Less abundant species included Talinum parviflorum, Carex flacca, Sedum stenopetalum, and Sedum divergens, which all exhibited 0 or near 0 AC regardless of depth or solar radiation levels. With the exception of T. calycinum, native species were less abundant than non-native species.In the second study, six common extensive green roof species of Sedum established from seed in May 2005 on a 10.0 cm (3.9 in) substrate depth were compared in both sun and shade over four growing seasons. AC was evaluated as in the previous study. Solar radiation did not affect AC, but overall species composition differed between sun and shade levels. The most abundant species in full sun were S. acre (0.57 AC) and S. album ‘Coral Carpet’ (0.51 AC). Sedum kamtschaticum (0.57 AC) and Sedum spurium ‘Coccineum’ (0.35 AC) performed the best in the shade. For both solar levels, the least abundant species at week 174 were Sedum pulchellum (0.0 AC) and S. album ‘Coral Carpet’ (0.1 AC). 相似文献