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Pregnancy‐associated glycoproteins (PAG) are secreted by the binucleate giant cells of the ruminant placenta and enter maternal circulation at the time of placental attachment. The IDEXX Milk Pregnancy Test (IDEXX, Westbrook, ME) detects a subset of PAG in milk. Although designed as a management tool for dairy cows, there is potential for using the milk PAG test in beef cows. Our objective was to compare the performance of the milk PAG ELISA with a gold standard method for pregnancy diagnosis and determine the agreement between milk and serum PAG analysis in lactating beef cows. Angus and Angus‐crossed cows (n = 332) from two Michigan beef herds were enrolled in this study. Cows were subjected either to timed artificial insemination followed by exposure to a bull or exclusively exposed to a bull. The bulls and cows were separated 30 days prior to examination. Serum and milk samples were collected and submitted within 24 h of collection to a commercial laboratory for PAG analysis using the IDEXX Milk Pregnancy Assay (milk) and the IDEXX Bovine Pregnancy Assay (serum). Concurrently with milk and serum collection, each cow was examined transrectally by palpation or ultrasonography. When compared to transrectal examination, the performance (and 95% confidence intervals) of the milk PAG ELISA was sensitivity of 99.7% (99.0–100.0%) and specificity of 80.8% (65.6–95.9%). The lower specificity is likely due to the low prevalence (9.9%) of open cows (n = 30) in the herds examined. Of the 332 cows examined, 1.8% (n = 6) were classified as rechecks using the milk PAG ELISA. Results of the milk and serum PAG ELISA were in high agreement (kappa coefficient = 0.91). The milk PAG ELISA was accurate in predicting pregnancy status using milk collected from beef cattle between days 37 and 125 post‐insemination and may be useful for aiding management decisions in beef herds. 相似文献
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Factors affecting pregnancy rate in Holstein-Friesian cattle mated during summer in a tropical upland environment 总被引：1，自引：0，他引：1
SUMMARY The relationships between some environmental factors and reproductive rates of dairy cattle during summer in a tropical upland area were studied using records for Holstein-Frieslan milking cows and non-lactating growing helfers maintained at the Kalri Research Station on the Atherton Tableland in north Queensland. The Station is situated in a tropical upland environment with warm, wet summers and dry, cool winters. Data were collected over a 12-year-period (1977 to 1988) for cows grazing nitrogen (N)-fertilised grass pastures and receiving either no supplement or about 3.5 kg molasses/cow/day. Cattle were mated over a 10-week period starting from mid-January to early February, with 32 to 36 cows and 5 to 42 heifers used in each year. There was considerable variation among years in the pregnancy rate after 3 Inseminations, with a low of 39% and a high of 94% pregnancy. This variation from year to year was reduced for cows grazing pastures receiving 300 kg N/ha/yr after molasses feeding commenced in 1984–85. Pregnancy rates after molasses Introduction were higher for cows grazing pastures receiving 300 compared with 100 kg N/ha/yr. Pregnancy rate in cows and heifers was reduced from 80 to 55% as mean daily maximum temperature increased from 26°C to 27.5°C. At mean maximum temperatures above 27°C for cows and 27.6°C for heifers pregnancy rates to 3 Inseminations were consistently below 60%. Much of the year to year variation in pregnancy rate was related to rainfall during the mating period. There was a positive association between pregnancy rate and rainfall up to 1000 mm, and this was associated with differences in growth rate of pasture. In years of very high rainfall, above 1500 mm, there was a depression in pregnancy rate, which may be related to the intense humidity in very wet years causing heat stress in cows and associated problems such as feet infections, or a deterioration in pasture quality. We conclude that much of the variation in pregnancy rate during summer may have been related to heat stress and the level of feed supply in the form of pasture and supplements. 相似文献
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Megacolon in two related Clydesdale foals 总被引：1，自引：0，他引：1
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MW PATON SS SUTHERLAND† IR ROSE RA HART† AR MERCY† TM ELLIS† 《Australian veterinary journal》1995,72(7):266-269
SUMMARY The decrease in the prevalence of Corynebacterium pseudotuberculosis after two generations of vaccination against the disease it causes, was used to estimate the rate of control of caseous lymphadenitis (CLA). Three groups of 150 sheep, of which 50 in each group were artificially infected with C pseudotuberculosis and 100 in each group were uninfected sheep, were run separately for 40 months and shorn 5 times to promote the spread of CLA. One lot of 50 infected sheep and 2 lots of 100 uninfected sheep were vaccinated against CLA. The rate of spread of CLA was recorded. Sheep vaccinated against CLA and naturally exposed to infection had a 74% lower infection rate than unvaccinated sheep. Sheep vaccinated against CLA and exposed to only vaccinated infected sheep had a 97% lower infection rate. Unvaccinated sheep had a 76% infection rate, with 77% of the transmission occurring at the 4th and 5th shearings, without any discharging CLA abscesses being observed. This study supports the view that in Australian wool producing flocks, CLA spreads mainly from sheep with discharging lung abscesses to sheep with shearing cuts. Vaccinated sheep infected with CLA have 96% fewer lung abscesses compared with unvaccinated infected sheep and are therefore less likely to spread this disease to other sheep . 相似文献
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MW PATON IR ROSE RA HART† SS SUTHERLAND† AR MERCY† TM ELLIS† JA DHALIWAL‡ 《Australian veterinary journal》1994,71(2):47-49
SUMMARY The effect of natural Corynebacterium pseudotuberculosis infection on wool production and quality in sheep was examined in light of evidence that artificial C pseudotuberculosis infection causes wool production loss. A toxin ELISA was used to identify sheep that had been infected with C pseudo tuberculosis. Greasy and clean fleece weights and fibre diameter were compared in infected and uninfected sheep. C pseudotuberculosis infection caused a 3.8 to 4.8% decrease in greasy wool production and a 4.1 to 6.6% decrease in clean wool production. C pseudotuberculosis infection did not affect fibre diameter. The effects of caseous lymphadenitis (the disease caused by C pseudotuberculosis infection) cause an annual loss of about $17 million in wool production to the Australian wool industry. 相似文献
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F Hill PB Stark RT Stebbins ER Anderson HM Antia TM Brown TL Duvall DA Haber JW Harvey DH Hathaway R Howe RP Hubbard HP Jones JR Kennedy SG Korzennik AG Kosovichev JW Leibacher KG Libbrecht JA Pintar EJ Rhodes J Schou MJ Thompson S Tomczyk CG Toner R Toussaint WE Williams 《Science (New York, N.Y.)》1996,272(5266):1292-1296
The Global Oscillation Network Group (GONG) project estimates the frequencies, amplitudes, and linewidths of more than 250,000 acoustic resonances of the sun from data sets lasting 36 days. The frequency resolution of a single data set is 0.321 microhertz. For frequencies averaged over the azimuthal order m, the median formal error is 0.044 microhertz, and the associated median fractional error is 1.6 x 10(-5). For a 3-year data set, the fractional error is expected to be 3 x 10(-6). The GONG m-averaged frequency measurements differ from other helioseismic data sets by 0.03 to 0.08 microhertz. The differences arise from a combination of systematic errors, random errors, and possible changes in solar structure. 相似文献