全文获取类型
| 收费全文 | 141篇 |
| 免费 | 14篇 |
专业分类
| 林业 | 8篇 |
| 农学 | 2篇 |
| 基础科学 | 1篇 |
| 30篇 | |
| 综合类 | 12篇 |
| 农作物 | 14篇 |
| 水产渔业 | 26篇 |
| 畜牧兽医 | 45篇 |
| 园艺 | 6篇 |
| 植物保护 | 11篇 |
出版年
| 2023年 | 1篇 |
| 2022年 | 3篇 |
| 2021年 | 4篇 |
| 2020年 | 6篇 |
| 2019年 | 12篇 |
| 2018年 | 13篇 |
| 2017年 | 10篇 |
| 2016年 | 7篇 |
| 2015年 | 8篇 |
| 2014年 | 16篇 |
| 2013年 | 10篇 |
| 2012年 | 11篇 |
| 2011年 | 11篇 |
| 2010年 | 2篇 |
| 2009年 | 3篇 |
| 2008年 | 5篇 |
| 2007年 | 7篇 |
| 2006年 | 3篇 |
| 2005年 | 5篇 |
| 2004年 | 3篇 |
| 2003年 | 3篇 |
| 2002年 | 3篇 |
| 1997年 | 1篇 |
| 1993年 | 2篇 |
| 1990年 | 1篇 |
| 1988年 | 1篇 |
| 1987年 | 1篇 |
| 1980年 | 1篇 |
| 1979年 | 1篇 |
| 1973年 | 1篇 |
排序方式： 共有155条查询结果，搜索用时 812 毫秒
91.
Halina Wi?niewska Maria Surma Karolina Krystkowiak Tadeusz Adamski Anetta Kuczyńska Piotr Ogrodowicz Krzysztof Miko?ajczak Jolanta Belter Maciej Majka Zygmunt Kaczmarek Pawe? Krajewski Aneta Sawikowska Leszek Lenc Anna Baturo-Cie?niewska Aleksander ?ukanowski Tomasz Góral Czes?aw Sadowski 《Breeding Science》2016,66(2):281-292
Fusarium head blight (FHB), caused by the fungal plant pathogen Fusarium, is a fungal disease that occurs in wheat and can cause significant yield and grain quality losses. The present paper examines variation in the resistance of spring wheat lines derived from a cross between Zebra and Saar cultivars. Experiments covering 198 lines and parental cultivars were conducted in three years, in which inoculation with Fusarium culmorum was applied. Resistance levels were estimated by scoring disease symptoms on kernels. In spite of a similar reaction of parents to F. culmorum infection, significant differentiation between lines was found in all the analyzed traits. Seven molecular markers selected as linked to FHB resistance QTLs gave polymorphic products for Zebra and Saar: Xgwm566, Xgwm46, Xgwm389, Xgwm533, Xgwm156, Xwmc238, and Xgwm341. Markers Xgwm389 and Xgwm533 were associated with the rate of Fusarium-damaged kernels (FDK) as well as with kernel weight per spike and thousand kernel weight in control plants. Zebra allele of marker Xwmc238 increased kernel weight per spike and thousand kernel weight both in control and infected plants, whereas Zebra allele of marker Xgwm566 reduced the percentage of FDK and simultaneously reduced the thousand kernel weight in control and infected plants. 相似文献
92.
93.
Kuźmińska-Bajor M Kuczkowski M Grzymajło K Wojciech Ł Sabat M Kisiela D Wieliczko A Ugorski M 《Veterinary microbiology》2012,158(1-2):205-210
To investigate the role of non-hemagglutinating type 1 fimbriae in the pathogenesis of Salmonella Gallinarum, the isogenic mutant elaborating type 1 fimbriae with mannose-sensitive (MS) variant of the FimH adhesin from Salmonella Enteritidis and the mutant strain with no FimH expression were constructed. Their binding to chicken leukocytes in vitro and invasiveness in 1-day-old chicks were studied. Our results demonstrated that S. Gallinarum type 1 fimbriae with an endogenous variant of the FimH adhesin mediated mannose-resistant (MR) binding to avian leukocytes and did not bind to human epithelial cells. However, after allelic replacement of the FimH, mutated fimbriae with S. Enteritidis variant of the FimH adhesin bound to both cell types in a mannose-dependent manner. In chick model, S. Gallinarum expressing wild-type FimH variant colonized cecal tonsils and bursa of Fabricius more effectively and invaded the spleen and liver in greater numbers than S. Gallinarum fimH knockout strain or mutant expressing MS FimH variant from S. Enteritidis. The invasive potential of the latter was greatly reduced in chicks since no viable bacteria expressing MS variant of the adhesin could be recovered from intestinal lymphoid tissues or liver over a 6 days course of infection. Together, these results demonstrate that the S. Gallinarum type 1 fimbriae with the endogenous MR variant of the FimH protein increase systemic dissemination of S. Gallinarum and colonization of internal organs in chicks indicating the importance of these adhesive structures in the virulence of S. Gallinarum. 相似文献
94.
The influence of breed,age, gender,training level and ambient temperature on forelimb and back temperature in racehorses 
下载免费PDF全文
下载免费PDF全文 Maria Soroko Kevin Howell Krzysztof Dudek Radomir Henklewski Paulina Zielińska 《Animal Science Journal》2017,88(2):347-355
A previous thermographic study of racehorses identified 13 regions of interest (ROIs) for monitoring the impact of training. However, that investigation did not consider the influence of breed, age, gender or training intensity level on the temperature of ROIs. The present study adopted a multivariate analysis approach to determine whether the aforementioned factors, along with ambient temperature, significantly influenced ROI temperature in the key body regions. Thermography measurements were obtained from 53 racehorses of three breeds. Horses were in regular training for over 10 months, having 13 thermographic examinations in each racing season. Backward stepwise multiple linear regression indicated that ambient temperature and breed contributed significantly to the model for predicting ROI temperature at all 13 ROIs. Training intensity level contributed significantly to the model only at the thoracic vertebrae, the left third metacarpal bone and left fetlock joint. Neither gender nor age contributed to the model significantly at any ROI. Our data suggest that ambient temperature, breed and training level affect racehorse body surface temperature in some areas of the distal parts of the forelimbs and the back. This contributes to a better understanding of the normal range of thermographic findings in racehorses undergoing intensive training. 相似文献
95.
Serum concentrations of allergen‐specific IgE in horses with equine recurrent airway obstruction and healthy controls assessed by ELISA 下载免费PDF全文
下载免费PDF全文 96.
Katarzyna Basinska Krzysztof Marycz Agnieszka ?mieszek Jakub Nicpoń 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):113-120
A main symptom of equine metabolic syndrome (EMS) in ponies is pathological obesity characterized by abnormal accumulation of fat deposits and inflammation. In this study, we analyzed the expression of two pro-inflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), in subcutaneous adipose tissue and the correlation with serum concentrations in peripheral blood of Welsh ponies. Based on clinical examination findings, the animals were divided into two groups: ponies affected with EMS (n = 8) and obese ponies (n = 8). The adipose tissue was examined using immunohistochemical analysis while concentrations IL-6 and TNF-α were measured using enzyme-linked immunosorbent assays (ELISAs). Additionally, histological characterization of the adipose tissue was performed. The results obtained showed that IL-6 expression in adipose tissue biopsies derived from animals with EMS was enhanced while TNF-α levels of both groups were comparable. Compared to the obese ponies, EMS animals also had significantly elevated levels of serum IL-6 and TNF-α. Histological analysis revealed macrophage infiltration and fibrosis in adipose tissue preparations from the EMS group. These data suggest that IL-6 may play a key role in the course of EMS in Welsh ponies. Our findings also demonstrated that analysis of pro-inflammatory cytokines levels in serum may serve as an additional tool for diagnosing EMS. 相似文献
97.
Krzysztof Kucypera Marcin Lipowczan Anna Piekarska-Stachowiak Jerzy Nakielski 《Plant methods》2017,13(1):110
Background
The development of cell pattern in the surface cell layer of the shoot apex can be investigated in vivo by use of a time-lapse confocal images, showing naked meristem in 3D in successive times. However, how this layer is originated from apical initials and develops as a result of growth and divisions of their descendants, remains unknown. This is an open area for computer modelling. A method to generate the surface cell layer is presented on the example of the 3D paraboloidal shoot apical dome. In the used model the layer originates from three apical initials that meet at the dome summit and develops through growth and cell divisions under the isotropic surface growth, defined by the growth tensor. The cells, which are described by polyhedrons, divide anticlinally with the smallest division plane that passes depending on the used mode through the cell center, or the point found randomly near this center. The formation of the surface cell pattern is described with the attention being paid to activity of the apical initials and fates of their descendants.Results
The computer generated surface layer that included about 350 cells required about 1200 divisions of the apical initials and their derivatives. The derivatives were arranged into three more or less equal clonal sectors composed of cellular clones at different age. Each apical initial renewed itself 7–8 times to produce the sector. In the shape and location and the cellular clones the following divisions of the initial were manifested. The application of the random factor resulted in more realistic cell pattern in comparison to the pure mode. The cell divisions were analyzed statistically on the top view. When all of the division walls were considered, their angular distribution was uniform, whereas in the distribution that was limited to apical initials only, some preferences related to their arrangement at the dome summit were observed.Conclusions
The realistic surface cell pattern was obtained. The present method is a useful tool to generate surface cell layer, study activity of initial cells and their derivatives, and how cell expansion and division are coordinated during growth. We expect its further application to clarify the question of a number and permanence or impermanence of initial cells, and possible relationship between their shape and oriented divisions, both on the ground of the growth tensor approach.98.
Baran Agnieszka Mierzwa-Hersztek Monika Urbaniak Magdalena Gondek Krzysztof Tarnawski Marek Szara Magdalena Zieliński Marek 《Journal of Soils and Sediments》2020,20(6):2588-2597
Purpose
The purpose of the study was to determine the levels of polychlorinated dibenzo-p-dioxin and polychlorinated dibenzofuran (PCDD/F), two types of persistent organic pollutant (POP), in an urban retention reservoir located in an industrial zone within a coal-mining region. It also assesses the potential ecological risk of the PCDDs/Fs present in bottom sediments and the relationship between their content and the fraction of organic matter.
Materials and methodsThe sediment samples were collected from Rybnik Reservoir, located in the centre of the Rybnik Coal Region, Silesia, one of Poland’s major industrial centres. Seventeen PCDD/F congeners in the surface of the sediments were analysed using high-resolution gas chromatography and high-resolution mass spectrometry (HRGC/HRMS).
Results and discussionThe toxic equivalency (TEQ) of the PCDDs/Fs in the sediments ranged from 1.65 to 32.68 pg TEQ g?1. PCDDs constituted 59–78% of the total PCDDs/Fs, while the PCDFs accounted for 22–41%. The pattern of PCDD/F congeners in the sediments was dominated by OCDD. However, the second-most prevalent constituents were OCDF and ∑HpCDFs in the low TOC sediment (< 10 g TOC kg?1), but HpCDD in the rich TOC samples (> 10 g TOC kg?1). PCDD/F concentrations in the sediment samples were 2- to 38-fold higher than the sediment quality guidelines limit, indicating high ecological risk potential. Although a considerable proportion of PCDDs/Fs in the bottom sediments from the Rybnik Reservoir were derived from combustion processes, they were also obtained via transport, wastewater discharge, high-temperature processes and thermal electricity generation. The PCDD/F concentrations were significantly correlated with all fractions of organic matter; however, the strongest correlation coefficients were found between PCDDs/Fs and humic substances. Besides organic matter, the proportions of silt/clay fractions within sediments played an important role in the transport of PCDDs/Fs in bottom sediments.
ConclusionsThe silt/clay fraction of the bottom sediments plays a dominant role in the movement of PCDDs/Fs, while the organic matter fraction affects their sorption. The results indicate that the environmental behaviour of PCDDs/Fs is affected by the quantity and quality of organic matter and the texture of sediments.
相似文献99.
Czubinski J Dwiecki K Siger A Kachlicki P Neunert G Lampart-Szczapa E Nogala-Kalucka M 《Journal of agricultural and food chemistry》2012,60(7):1830-1836
Lupin seed globulin proteins form complexes with flavonoids, predominantly apigenin C-glycosides. Enzymes typical for the gastrointestinal tract were used to hydrolyze lupin seed globulins. Release of native flavonoids as a result of the proteolysis reaction was observed. Different analytical methods such as size exclusion chromatography, HPLC-MS, and fluorescence spectroscopy (steady-state fluorescence, fluorescence anisotropy, fluorescence lifetimes) were used for a detailed characterization of this phenomenon. Flavonoids liberated from lupin globulin proteins as a result of pancreatin-catalyzed digestion were bound by γ-conglutin resistant to this enzyme. Two possible mechanisms of this interaction may be suggested: hydrogen bonding between oligosaccharide chains of glycoproteins and the sugar moieties of the flavonoid glycosides or electrostatic attraction between positively charged γ-conglutin and flavonoids partially ionized at pH 7.5. 相似文献
100.
Water soluble arabinoxylans, especially of rye origin, are known to considerably improve quality of bread. The aim of the current research was to check the changes in properties of rye dough and bread after the addition of different arabinoxylan preparations. Structure forming properties of these additives were given by the application of hydrolysis, and cross-linking, which influenced molar mass and reactivity of arabinoxylans. 相似文献