Comparative analysis of inherited patterns of the transgene in transgenic mud loach Misgurnus mizolepis lines carrying the CAT reporter gene   总被引：1，自引：0，他引：1  

Dong Soo  KIM  Bong-Seok  KIM  Sang Jun  LEE  In-Seok  PARK  Yoon Kwon  NAM 《Fisheries Science》2004,70(2):201-210

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The mode of seed formation in Allium senescens and two Korean Allium species     

B. J. Kim    Y. C. Kwon    Y. H. Kwack    M. S. Lim  E. H. Park 《Plant Breeding》1999,118(5):435-438

The genus Allium includes several important vegetable and ornamental species, and is an economically important crop. The objective of this study was to determine the mode of reproduction in A. senescens L. (2n=6x= 48) and two Allium species collected in Korea. Chromosome numbers of embryo sac mother cells (EMCs) at diplotene of meiosis were 2n = 48 and unreduced in A. senescens. However, normal sexual reproduction was observed at diplotene in both A. senescens var. minor (2n = 2x= 16) and A. nutans L. (2n=4x= 32). Parthenogenetic embryogenesis also occurred in A. senescens at high frequencies among the three Allium species. Antipodal embryos in A. nutans were formed in unpollinated flowers. Normal seeds were not formed in flowers of A. senescens that had been emasculated and kept in sealed conditions. However, normal seeds were formed in the flowers that were pollinated 2 days after anthesis. In conclusion, diplospory, parthenogenesis, and pseudogamy occur in A. senescens.  相似文献   

Relationship between genetic divergence and hybrid performance in japonica rice grown in a cold water-irrigated field     

Soo-Jin Kwon  Sang-Nag Ahn  Eung-Gee Jeong  Yong-Hee Jeon  Hung-Goo Hwang  Hae-Chune Choi  Huhn-Pal Moon 《Euphytica》2002,128(3):389-396

The present study was carried out to evaluate genetic divergence among eleven japonica rice cultivars and to assess the relationship between genetic distance and hybrid performance in partial non-reciprocal crosses among them. The 44 F1 hybrids along with the eleven parents were evaluated for five cold tolerance-related traits; discoloration at seedling stage, days to heading, culm length, fertility, and spikelets per panicle in a cold water screening nursery (17 ^°C). The eleven parents were examined for DNA polymorphism using amplified fragment length polymorphisms(AFLPs). A total of 855 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.023 to0.524. Very little heterosis was observed in hybrids for most of the traits,whereas heterosis was high for fertility. The correlation values of GDs with F1 performance were mostly non-significant except for discoloration and fertility. The correlations of GDs with mid-parent and better-parent heterosis were not significant and proved to be of no predictive value. Our results indicate that GDs based on AFLP markers are not useful for predicting heterosis for cold tolerance in japonica hybrids. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Patient-specific embryonic stem cells derived from human SCNT blastocysts     

Hwang WS  Roh SI  Lee BC  Kang SK  Kwon DK  Kim S  Kim SJ  Park SW  Kwon HS  Lee CK  Lee JB  Kim JM  Ahn C  Paek SH  Chang SS  Koo JJ  Yoon HS  Hwang JH  Hwang YY  Park YS  Oh SK  Kim HS  Park JH  Moon SY  Schatten G 《Science (New York, N.Y.)》2005,308(5729):1777-1783

Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, nuclear transfer (NT)-hESCs, grown on human feeders from the same NT donor or from genetically unrelated individuals, were established at high rates, regardless of NT donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and matched the NT patient's DNA. The major histocompatibility complex identity of each NT-hESC when compared to the patient's own showed immunological compatibility, which is important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains to be done regarding the development of reliable directed differentiation and the elimination of remaining animal components. Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated.  相似文献   

Adsorption of Ammonium Nitrogen and Phosphate onto Basanite and Evaluation of Toxicity     

Choi  Jae-Woo  Ryu  Jae-Chun  Kwon  Kyu-Sang  Song  Mee-Kyung  Lee  Soonjae  Kim  Song-Bae  Lee  Sang-Hyup 《Water, air, and soil pollution》2014,225(8):1-11

Water, Air, &; Soil Pollution - Preliminary analysis on dredged marine sediments from Benoi basin in Singapore was carried out showing elevated concentrations of Zn, Cu, Pb, Cd, Cr and Ni....  相似文献   

Site-Specific Herbicide Decision Model to Maximize Profit in Winter Wheat     

Young  Douglas L.  Kwon  T. J.  Smith  E. G.  Young  F. L. 《Precision Agriculture》2003,4(2):227-238

A user-friendly computerized agricultural herbicide decision model has been developed for selecting profitable site-specific herbicide applications in winter wheat. The model is based on 6 years of field research in southeastern Washington State, USA. The model calibrates herbicide applications to management unit weed densities, soil organic matter, soil moisture, and preceding management, as well as to expected input and output prices. The model increased broadleaf herbicide rates by an average 0.65 of label rates compared to the recommendations by farmers and weed science professionals, but cut the more expensive grass herbicides by an average 0.56 of label rates. The model increased average projected profitability, excluding model application costs, by 65% compared to four other criteria for determining application rates. The profitability increase relative to local farmers was 19%. Both the model and the cooperating farmers properly chose to use no grass herbicides on the study sites, but the weed science experts chose to use up to 1.0 of label rates. The estimated payoff from using the model substantially exceeded the cost of weed scouting and other information collection. Determining economically optimal sampling and management units is an important challenge for the adoption of precision agriculture models like the one developed in this study.  相似文献   

Three-dimensional porous collagen/chitosan complex sponge for tissue engineering     

Sung Eun Kim  Yong Woo Cho  Eun Jung Kang  Ick Chan Kwon  Eunhee Bae Lee  Jung Hyun Kim  Hesson Chung  Seo Young Jeong 《Fibers and Polymers》2001,2(2):64-70

A three-dimensional, porous collagen/chitosan complex sponge was prepared to closely simulate basic extracellular matrix (ECM) constitutes, collagen and glycosaminoglycan. The complex sponge was prepared by a lyophilization method and had the regular network with highly porous structure, suitable for cell adhesion and growth. The pores were well interconnected, and their distribution was fairly homogeneous. The complex sponge was crosslinked using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to increase its biological stability and enhance its mechanical properties. The crosslinking medium had a great effect on the inner structure of the sponge. The homogeneous, porous structure of the sponge was remarkably collapsed in an aqueous crosslinking medium. However, the morphology of the sponge remained almost intact in a water/ethanol mixture crosslinking milieu. Mechanical properties of the collagen/chitosan sponge were significantly enhanced by EDC-mediated crosslinking. The potential of the sponge as a scaffold for tissue engineering was investigated using a Chinese hamster ovary cell (CHO-K1) line.  相似文献   

Partial sequence of the spike glycoprotein gene of transmissible gastroenteritis viruses isolated in Korea   总被引：1，自引：0，他引：1  

Kim SJ  Han JH  Kwon HM 《Veterinary microbiology》2003,94(3):195-206

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Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR     

Hyoung Joon Moon  Hye Kwon Kim  Seong Jun Park  Chul Seung Lee  Dae Sub Song  Bo Kyu Kang  Bong Kyun Park 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(4):317-322

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Synergistic effect of ERK inhibition on tetrandrine-induced apoptosis in A549 human lung carcinoma cells     

Hyun Sun Cho  Seung Hee Chang  Youn Sun Chung  Ji Young Shin  Sung Jin Park  Eun Sun Lee  Soon Kyung Hwang  Jung Taek Kwon  Arash Minai Tehrani  Minah Woo  Mi Sook Noh  Huda Hanifah  Hua Jin  Cheng Xiong Xu  Myung Haing Cho 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(1):23-28

Tetrandrine (TET), a bis-benzylisoquinoline alkaloid from the root of Stephania tetrandra, is known to have anti-tumor activity in various malignant neoplasms. However, the precise mechanism by which TET inhibits tumor cell growth remains to be elucidated. The present studies were performed to characterize the potential effects of TET on phosphoinositide 3-kinase/Akt and extracellular signal-regulated kinase (ERK) pathways since these signaling pathways are known to be responsible for cell growth and survival. TET suppressed cell proliferation and induced apoptosis in A549 human lung carcinoma cells. TET treatment resulted in a down-regulation of Akt and ERK phosphorylation in both time-/concentration-dependent manners. The inhibition of ERK using PD98059 synergistically enhanced the TET-induced apoptosis of A549 cells whereas the inhibition of Akt using {"type":"entrez-nucleotide","attrs":{"text":"LY294002","term_id":"1257998346","term_text":"LY294002"}}LY294002 had a less significant effect. Taken together, our results suggest that TET: i) selectively inhibits the proliferation of lung cancer cells by blocking Akt activation and ii) increases apoptosis by inhibiting ERK. The treatment of lung cancers with TET may enhance the efficacy of chemotherapy and radiotherapy and increase the apoptotic potential of lung cancer cells.  相似文献