Rose (Rosa hybrida L.) EST-derived microsatellite markers and their transferability to strawberry (Fragaria spp.)     

Young Hoon Park  Seong Gyu Ahn  Young Mi Choi  Hye Jeong Oh  Dong Chun Ahn  Jin Gi Kim  Jum Soon Kang  Young Whan Choi  Byoung Ryong Jeong 《Scientia Horticulturae》2010

The ‘Genome database for Rosaceae (GDR)’ provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei–Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King’ and ‘Carnival’) to 0.99 (‘First Red’ and ‘Red Champ’). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang’ and ‘Janghyee’) to 0.64 (‘Janghyee’ and ‘Pragana’). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana’ was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae.  相似文献   

Effects of Xijiao Dihuang and Yinqiao San decoction on ICAM-1 and VCAM-1 expression in mouse lung tissues and rat pulmonary microvascular endothelial cells infected with influenza virus     

ZHANG Chen-yue  ZHANG Shu  WU Ying  KIM Ye-ji  LI Gen-mao  WANG Qian  HAO Yu 《园艺学报》2013,29(9):1685-1690

AIM：To investigate the effects of Xijiao Dihuang and Yinqiao San decoction (XDY) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in mouse lung tissues and rat pulmonary microvascular endothelial cells (RPMVECs) infected with influenza virus, and to explore its mechanism for treatment of viral pneumonia. METHODS：Fifty-four male BALB/c mice were randomly divided into normal group, model group and XDY group (n=18 in each group). The viral pneumonia model was established by intranasally dripping influenza A (H1N1) virus into the mice. The mice in XDY group were treated with XDY 1 h after dripping the virus. The expression of ICAM-1 and VCAM-1 in lung tissues was examined by immunohistochemical staining 2, 4 and 6 d after infection. On the other hand, RPMVECs were obtained from male Wistar rats and primarily cultured. The cells were randomly divided into control group, virus group, virus+XDY group, tumor necrosis factor α (TNF-α) group and TNF-α+XDY group. The mRNA and protein expression of ICAM-1 and VCAM-1 was evaluated by real-time PCR and flow cytometry 24 h after infection. RESULTS：Virus exposure increased ICAM-1 and VCAM-1 expression in mouse lung tissues (P<0.01), and XDY treatment attenuated this effect (P<0.01). Virus and TNF-α both led to the increases in mRNA and protein expression of ICAM-1 and VCAM-1 in RPMVECs (P<0.01), which were also reduced by treatment with XDY (P<0.01). CONCLUSION：Treatment with XDY decreases virus-induced ICAM-1 and VCAM-1 expression, suggesting an important role of XDY in treatment of viral pneumonia.  相似文献   

In-vitro and in-vivo binding activity of chicken egg yolk immunoglobulin Y (IgY) against gliadin in food matrix     

Gujral N  Löbenberg R  Suresh M  Sunwoo H 《Journal of agricultural and food chemistry》2012,60(12):3166-3172

Chicken egg yolk immunoglobulin Y (IgY) is a promising alternative for the prevention of enteric gliadin absorption, the predisposing factor of celiac disease (CD). IgY antibody was produced from the egg yolk of Single Comb White Leghorn chickens during the immunization period for the development of an oral immunotherapeutic agent. Here, we report the potential use of spray dried IgY antibody formulation using sugar protectants (mannitol, sorbitol, or microcrystalline cellulose powder (MCCP)). The long-term stability of the spray dried egg yolk powder formulated with 37.5% mannitol (EYP-M) preserved IgY antibody activity at 99.9%, which was significantly higher than that with other protectants (p < 0.05). In a dissolution test, the EYP-M shows 82.4% IgY activity after 2 h in simulated gastric fluid (SGF). A competitive ELISA at 50% inhibition (IC(50)) shows that 1.6 mg/mL EYP-M bound to 7.6 mg/mL and 10.5 mg/mL gliadin in SGF without and with food matrix conditions, respectively, whereas in simulated intestinal fluid, the formulation bound to 10 mg/mL gliadin, regardless of a food matrix. In-vivo study: BALB/c mice fed with EYP-M and gliadin at a ratio of 1:5 (w/w) demonstrated that gliadin absorption in the gastrointestinal tract was minimal at <1%. Thus, EYP-M containing IgY antibody may be used in CD patients to eliminate the effects of ingested toxic gliadin.  相似文献   

Different response of silicate fertilizer having electron acceptors on methane emission in rice paddy soil under green manuring     

Chang?Hoon?Lee  Sang?Yoon?Kim  Maria?B.?Villamil  Prabhat?Pramanik  Chang?Ok?Hong  Pil?Joo?KimEmail author 《Biology and Fertility of Soils》2012,48(4):435-442

Cultivation of green manure plants during the fallow season in rice paddy soil has been strongly recommended to improve soil properties. However, green manuring may impact greenhouse gas emission, methane (hereafter, CH₄) in particular, under the flooded rice cultivation and thus, application of chemical amendments being electron acceptors can be an effective mitigation strategy to reduce CH₄ emissions in irrigated rice (Oryza sativa L.) field amended with green manure. To investigate the effect of iron (Fe) slag silicate fertilizer (hereafter, silicate fertilizer), which was effective in reducing CH₄ emission and increasing rice productivity, in green manure-amended paddy soil, the aboveground biomass of Chinese milk vetch (hereafter, vetch) was added at rates of 0, 10, 20, and 40 Mg (fresh weight) ha⁻¹ before the application of silicate fertilizer, which was added at rates of 0 and 2.3 Mg ha⁻¹. Silicate fertilization reduced the seasonal CH₄ flux by ca. 14.5% and increased rice yield by ca. 15.7% in the control (no vetch application) treatment. However, CH₄ production was increased by silicate fertilization in vetch-treated soil particularly at the initial rice growing stage, which was probably due to the enhanced decomposition of added organic matters by the silicate liming effect. In conclusion, silicate fertilization is not effective in reducing CH₄ production in green manure-amended rice paddy soils and its use should be properly controlled.  相似文献   

Synthesis,characterization, and properties of ethoxylated azo dyes     

Kaihua?Shen  Dong?Hoon?ChoiEmail author  Zongshi?Li 《Fibers and Polymers》2003,4(1):32-37

The nonionic surfactant properties of ethoxylated azo dyes, such as cloud point and surface tension have been investigated. The synthesized ethoxylated azo dyes could dye polyester fabric without any special pre-treatment. When the average number of ethylene oxide (EO) in the ethoxylated azo dye reached 6, its fixation could exceed 90% from the thermosol dyeing process. The average degree of condensation of ethylene glycol in the chain was interrelated with the dyeing results and did not affect on the maximum absorption wavelength (λmax) of the polyoxyethylene dye. When the average length of polyoxyethylene chain decreased, the molecular weight of dyes became smaller and the fixation of dyes was improved.  相似文献   

Association between the body weight of growing pigs and the functional capacity of their gut microbiota     

Ju Kyoung Oh  Jong Pyo Chae  Edward Alain B. Pajarillo  Sung Hun Kim  Min‐Jin Kwak  Jong‐Su Eun  Seok Woo Chee  Kwang‐Youn Whang  Sae‐Hun Kim  Dae‐Kyung Kang 《Animal Science Journal》2020,91(1)

Gastrointestinal microbiota impact host's biological activities, including digestion of indigestible feed components, energy harvest, and immunity. In this study, fecal microbiota of high body weight (HW) and low body weight (LW) growing pigs at 103 days of age were compared. Principal coordinates analysis separated the HW and LW groups into two clusters, indicating their potential differences between microbial community composition. Although the abundances of two major phyla, Firmicutes and Bacteroidetes, did not significantly differ between the HW and LW groups, some genera showed significant differences. Among them, Peptococcus and Eubacterium exhibited strong positive correlations with body weight (BW) and average daily gain (ADG) (Rho > 0.40), whereas Treponema, Desulfovibrio, Parabacteroides, and Ruminococcaceae_unclassified exhibited strong negative correlations with BW and ADG (Rho < ?0.40). Based on these results, the structure of intestinal microbiota may affect growth traits in pigs through host–microbe interactions. Further in‐depth studies will provide insights into how best to reshape host–microbe interactions in pigs and other animals as well.  相似文献   

Comparison of native and citrated whole blood samples for rapid thromboelastography in Beagles     

Hyebin Wang  Aryung Nam  Kunho Song  Hwa Young Youn  Kyoung Won Seo 《Journal of Veterinary Emergency and Critical Care》2020,30(1):54-59

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Effect of soil pH on the sorption capacity of soil organic matter for polycyclic aromatic hydrocarbons in unsaturated soils     

Pil-Gon KIM  Abhrajyoti TARAFDAR  Jung-Hwan KWON 《土壤圈》2023,33(2):365-371

Sorption by soil organic matter (SOM) is considered the most important process affecting the bioavailability of hydrophobic organic chemicals (HOCs)in soil.The sorption capacity of SOM for HOCs is affected by many environmental factors.In this study,we investigated the effects of soil pH and water saturation level on HOC sorption capacity of SOM using batch sorption experiments.Values of soil organic carbon-water partition coefficient (K_OC) of six selected polycyclic aromatic hydrocar...  相似文献   

Induced resistance against a bacterial disease by orysastrobin,a chemical fungicide     

Lee  Jang Hoon  Anderson  Anne J.  Kim  Young Cheol 《European journal of plant pathology / European Foundation for Plant Pathology》2021,161(4):981-986

European Journal of Plant Pathology - Certain chemical pesticides control plant diseases through induction of systemic resistance mechanisms. In this study, orysastrobin, a “quinone outside...  相似文献   

Neoamphimedine circumvents metnase-enhanced DNA topoisomerase IIα activity through ATP-competitive inhibition     

Ponder J  Yoo BH  Abraham AD  Li Q  Ashley AK  Amerin CL  Zhou Q  Reid BG  Reigan P  Hromas R  Nickoloff JA  LaBarbera DV 《Marine drugs》2011,9(11):2397-2408

Type IIα DNA topoisomerase (TopoIIα) is among the most important clinical drug targets for the treatment of cancer. Recently, the DNA repair protein Metnase was shown to enhance TopoIIα activity and increase resistance to TopoIIα poisons. Using in vitro DNA decatenation assays we show that neoamphimedine potently inhibits TopoIIα-dependent DNA decatenation in the presence of Metnase. Cell proliferation assays demonstrate that neoamphimedine can inhibit Metnase-enhanced cell growth with an IC(50) of 0.5 μM. Additionally, we find that the apparent K(m) of TopoIIα for ATP increases linearly with higher concentrations of neoamphimedine, indicating ATP-competitive inhibition, which is substantiated by molecular modeling. These findings support the continued development of neoamphimedine as an anticancer agent, particularly in solid tumors that over-express Metnase.  相似文献