Acquired proximal renal tubulopathy in dogs exposed to a common dried chicken treat: retrospective study of 108 cases (2007–2009)     

MF Thompson  LM Fleeman  AE Kessell  LA Steenhard  SF Foster 《Australian veterinary journal》2013,91(9):368-373

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Application and Commercialization of Flow Cytometrically Sex-Sorted Semen   总被引：1，自引：0，他引：1  

D Rath  LA Johnson 《Reproduction in domestic animals》2008,43(S2):338-346

The current technology to sort X and Y chromosome bearing sperm population requires individual identification and selection of spermatozoa in a modified high-speed flow cytometer. For farm animal species, the technology is capable of producing sexed sperm at greater than 90% purity. However, only in the bovine, the technology has reached a developmental level that allows its commercial application. Meanwhile, the demand for female calves has grown rapidly, which encourages the demand for sex-sorted semen from high genetic value bulls. The success of the technology will depend mainly on the fertilizing capacity of the sorted spermatozoa, as this is the most affecting and economically relevant factor. To date, fertility is still variable and is quite dependant on post-sort processing. New processing techniques are under investigation and will likely be able to improve the fertility rates after AI with sex-sorted semen. It is of great importance to select the right bulls and to test the sorted samples on a routine basis. In addition to the demand for sex-sorted semen by the cattle industry, there is also a significant demand expressed by pig farmers. However, it is still unknown if the use of sex-sorted semen through commercial pig AI will be economically feasible. For the pig, the combination of in vitro fertilization with sexed semen and non-surgical embryo transfer is an alternative that merits further scientific attention. Recent developments in ovine AI and ET will make it very likely that commercial sheep industry will adopt the sexing technology in their breeding concepts.  相似文献   

Climate and vegetation history of the midcontinent from 75 to 25 ka: A speleothem record from crevice cave, missouri, USA   总被引：3，自引：0，他引：3  

JA Dorale  RL Edwards  E Ito  LA Gonzalez 《Science (New York, N.Y.)》1998,282(5395):1871-1874

Four Missouri stalagmites yield consistent overlapping records of oxygen and carbon isotopic changes and provide a climate and vegetation history with submillennial resolution from 75 to 25 thousand years ago (ka). The thorium-230-dated records reveal that between 75 and 55 ka, the midcontinental climate oscillated on millennial time scales between cold and warm, and vegetation alternated among forest, savanna, and prairie. Temperatures were highest and prairie vegetation peaked between 59 and 55 ka. Climate cooled and forest replaced grassland at 55 ka, when global ice sheets began to build during the early part of Marine Oxygen Isotope Stage 3.  相似文献   

Hunting behavior of a marine mammal beneath the antarctic fast Ice   总被引：1，自引：0，他引：1  

RW Davis  LA Fuiman  TM Williams  SO Collier  WP Hagey  SB Kanatous  S Kohin  M Horning 《Science (New York, N.Y.)》1999,283(5404):993-996

The hunting behavior of a marine mammal was studied beneath the Antarctic fast ice with an animal-borne video system and data recorder. Weddell seals stalked large Antarctic cod and the smaller subice fish Pagothenia borchgrevinki, often with the under-ice surface for backlighting, which implies that vision is important for hunting. They approached to within centimeters of cod without startling the fish. Seals flushed P. borchgrevinki by blowing air into subice crevices or pursued them into the platelet ice. These observations highlight the broad range of insights that are possible with simultaneous recordings of video, audio, three-dimensional dive paths, and locomotor effort.  相似文献   

半夏SSR分子标记开发与遗传多样性     

张景  李晓东  宗庆波  何贝贝  拉基  景纳良  王柯月  钟淑梅  舒少华 《华中农业大学学报》2021,40(6):19-26

对半夏转录组数据进行分析,设计开发SSR引物,利用筛选出的SSR引物分析不同居群间半夏的遗传多样性,为半夏分子标记辅助育种提供支撑。结果显示:筛选出的19对多态性较高的引物在17个半夏居群中共检测到49个多态性位点,具有较高的多态性;利用UPGMA作图共将17个半夏居群分为3个类群;通过分子方差分析(AMOVA)发现半夏居群内的变异达到88%,说明群体与群体间的差异相对较小,遗传变异主要来源于个体间;居群间平均分化系数Fst为0.124,居群间基因流Nm值为1.765,表明居群间能正常地进行基因交流,且居群间遗传分化受基因流的影响较大。本研究利用SSR技术开发的19对SSR引物在不同半夏居群间具有一定的通用性、多态性,能将17份半夏材料明确区分。  相似文献   

云南景东县豆科固氮树种及根瘤菌资源调查   总被引：1，自引：0，他引：1  

贾风勤  杨比伦  腊萍 《西南林学院学报》2009,29(4):13-18

对云南景东县豆科固氮树种进行调查采集与鉴定,确认并记录70种与相应的根瘤菌(菌株)共生的豆科固氮树种.其中,云实亚科1属1种,含羞草亚科6属20种,蝶形花亚科23属49种.根瘤大小不一,形状各异,有球形、椭圆形、棒状、珊瑚状;表面颜色有浅黄色、棕黑色、棕色或黑色.对以活体保存的材料进行根瘤菌的分离、鉴定,获得并保存了分别与其中38种豆科树种相共生的根瘤菌菌株147株.调查表明,在景东县及云南南亚热带区域和热带区域,不仅拥有可供在多方面开发利用的豆科固氮树种,且具有丰富的可供科研和开发利用的根瘤菌资源.  相似文献   

甜菜SSR反应体系优化及重要农艺性状分子标记   总被引：5，自引：0，他引：5  

代培红  腊萍  郭长奎  高文伟  章建新  罗淑萍 《新疆农业科学》2013,50(7):1199

[目的]为选育优质甜菜新品种,利用SSR法筛选与高糖、高产、耐盐相关的分子标注.[方法]研究对甜菜SSR-PCR反应体系进行优化,并利用SSR分子标记方法和分离群体分组分析法(Bulked SegregateAnalysis,BSA)对具有高糖/低糖、低产/高产、耐盐/不耐盐三种重要农艺性状的甜菜亲本和F2代植株进行分析.[结果]研究建立了适宜甜菜的SSR-PCR反应体系为:20μL反应体系中含l×Buffer、2.0 mmol/LMg2+、1.5 uTaq DNA聚合酶、0.20 mmol/L dNTP、1.5 μmol/L引物、60 ng DNA模板.依据优化体系,对不同农艺性状的甜菜亲本与F2代进行SSR-PCR扩增分析,高糖性状获得了200和100 bp两条与高糖性状连锁的标记,250和230 bp两条与高产性状连锁的标记,550、250和100 bp三条与耐盐性状紧密连锁的分子标记.[结论]研究获得的7条特异条带是与甜菜重要农艺性状连锁的分子标记,将为甜菜的育种工作提供重要的理论基础.  相似文献   

毕节地区植烟土壤肥力状况评价   总被引：3，自引：1，他引：2  

代昌明  腊贵晓  翟欣  陈文相  陈雪  喻奇伟  刘国顺 《江西农业学报》2011,23(8):9-11,16

2007～2008年对在毕节地区主要烟区采集的200个植烟土壤样品进行了土壤养分含量分析,结果表明:植烟土壤的有机质含量平均在27.70 g/kg,总体上处于适宜水平;pH值以偏酸性或中性为主;有效氮含量偏高;速效磷含量偏低;速效钾含量整体丰富,平均为158.42 mg/kg;土壤水溶性氯含量整体适宜。此外,针对土壤养分含量状况,提出了一些相应的改良措施。  相似文献   

芥蓝芥子油苷提取条件优化研究     

腊贵晓  孔海民  方萍  杨铁钢 《中国农业科技导报》2014,16(3):150-155

采用正交试验设计,研究了提取温度、提取剂甲醇浓度(V/V)、提取时间和待测物质与提取液比例(简称“物液比”) 对芥蓝菜薹中芥子油苷(总芥子油苷含量、总脂肪族芥子油苷和总吲哚族芥子油苷)提取效果的影响。结果表明,物液比和提取温度对总芥子油苷、总脂肪族芥子油苷和总吲哚族芥子油苷提取效果影响显著(P<0.05),而甲醇浓度和提取时间对芥子油苷提取率无显著影响(P>0.05)。综合考虑提取效果和成本,芥蓝中芥子油苷最佳提取条件为75℃,物液比1∶45(g∶mL),甲醇浓度70%,提取时间5 min。在该条件下,总芥子油苷提取量为8.31 μmol/g DW,总脂肪族芥子油苷提取总量为7.58 μmol/g DW,总吲哚族脂肪族芥子油苷提取总量为0.73 μmol/g DW。  相似文献   

绵羊LDHβ基因的生物信息学分析及其在不同剩余采食量绵羊肝脏和肌肉组织中的表达   总被引：1，自引：1，他引：0  

莫负涛  李发弟  喇永富  张小雪  唐德富  王维民 《甘肃农业大学学报》2017,52(3)

【目的】为鉴定绵羊乳酸脱氢酶β(lactate dehydrogenase B,LDHβ)基因的分子特征,研究其在不同剩余采食量绵羊肝脏和肌肉组织中的表达差异.【方法】测定了137只‘湖羊’公羔的剩余采食量(residual feed intake,RFI),按RFI进行排序,分别筛选出RFI最高(high-residual feed intake,H-RFI)和RFI最低(low-residual feed intake,L-RFI)的羊各15只,屠宰后,采集肝脏和肌肉组织,利用Q-PCR技术检测绵羊LDHβ基因分别在H-RFI和L-RFI羊肝脏和肌肉中表达量,并利用生物信息学软件构建了该基因的系统进化树和预测其结构与功能.【结果】绵羊LDHβ基因开放阅读框(open reading frame,ORF)为1 005bp,编码334个氨基酸,其蛋白质分子质量为36 479.43U,理论等电点为6.40.绵羊LDHβ基因与山羊的亲缘关系较近,其次为牛,序列同源性高.功能结构域预测结果显示,该基因编码产物在内质网中参与辅酶因子生物合成的可能性最高.Q-PCR结果显示,绵羊LDHβ-mRNA基因在L-RFI羊肝脏和肌肉中表达量均显著低于H-RFI羊(P0.01).【结论】绵羊LDHβ基因作为能量代谢的关键酶参与绵羊饲料效率的调控.  相似文献