Ian Bryant     

LA Fitzpatrick 《Australian veterinary journal》1993,70(8):320-320

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A role for DNA characterisation in ovine footrot     

DJ McGILLIVERY  JJ WEBBER  LA TOOHEY 《Australian veterinary journal》1989,66(4):106-108

Bacteroides nodosus involved in several outbreaks of ovine footrot over a number of years were subjected to DNA restriction endonuclease analysis. Individual isolates were found to have characteristic Bam HI profiles which permitted their accurate identification and differentiation from other isolates. Bam HI profiles of B. nodosus isolates were used in epidemiological investigations involving consecutive outbreaks of footrot on individual and neighbouring farms. The relationship of given isolates to a common source could be established by this means. Restriction endonuclease analysis provides an additional epidemiological tool in ovine footrot investigations as it accurately identifies interstrain differences in a manner not possible by conventional bacteriological and serological means.  相似文献   

表面活性剂辅助的甲硫氨酸和色氨酸叶面喷施对芥蓝生长及菜薹芥子油苷组分和含量的影响   总被引：1，自引：1，他引：0       下载免费PDF全文

腊贵晓  孔海民  方萍  杨铁钢 《中国生态农业学报》2014,22(4):439-446

采用盆栽试验,研究了脂肪族芥子油苷和吲哚族芥子油苷的合成前体甲硫氨酸和色氨酸单独及配合表面活性剂叶面喷施对芥蓝(Brassica alboglabra L.)生长及菜薹中芥子油苷组分和含量的影响。结果表明,各处理对芥蓝生长指标及芥子油苷组分无显著影响,在芥蓝菜薹中均检测到7种脂肪族芥子油苷和4种吲哚族芥子油苷。与喷施清水对照相比,甲硫氨酸、色氨酸单独叶面喷施及表面活性剂辅助的色氨酸叶面喷施,对芥蓝菜薹总芥子油苷含量、总脂肪族芥子油苷含量和总吲哚族芥子油苷含量均无显著影响。表面活性剂辅助下甲硫氨酸叶面喷施处理中菜薹总芥子油苷和总脂肪族芥子油苷含量比清水对照处理分别提高28.5%和31.9%,其中在11种芥子油苷中脂肪族芥子油苷3-丁烯基芥子油苷含量提高幅度最大,达到44.3%,同时表面活性剂辅助下甲硫氨酸叶面喷施显著提高了芥蓝菜薹全硫含量并降低了氮硫比(N/S)。本试验结果表明,有效供给甲硫氨酸能明显促进脂肪族芥子油苷的合成和积累,而辅之以表面活性剂能增进叶面对甲硫氨酸的吸收效果,其影响机理还有待进一步研究。  相似文献   

西藏草地资源价值及退化损失评估   总被引：3，自引：0，他引：3  

李忠魁  拉西 《中国草地学报》2009,31(2)

用4类共13个指标评估了西藏草地资源的价值,并根据草地资源退化速率评估了草地价值年损失量.结果表明,西藏草地资源的价值为1080.33×108元·a-1,包括:调节功能价值884.4989×108元·a-1(81.87%);实物提供价值173.2957×108元·a-1(16.04%);支持功能价值18.6253×108元·a-1(1.72%);旅游功能价值3.91×108元·a-1(0.36%).其中,调节功能的价值由6项组成(空气质量调节未计算),依大小次序排列为土壤碳累积、蓄水、营养物质循环、土壤保持、废弃物降解和养分归还.总体来看,草地资源的文化功能和支持功能有待进一步发展.西藏草地资源的总价值(现值)为11883.63亿元,退化损失的价值为2376.726亿元,年草地资源退化损失价值为297.091亿元.  相似文献   

胎儿期与成年期蒙古马骨骼肌肌纤维类型转化研究     

丁文淇  图格琴  任秀娟  陶克涛  拉希玛  贾紫洁  安塔娜  铁木齐尔·阿尔滕齐米克  韩海格  陶娜拉  芒来  白东义 《畜牧兽医学报》2022,53(1):88-99

为探索影响蒙古马胎儿期和成年期肌纤维类型差异机理.本研究选取3匹4月龄胎儿(两母一公)与3匹5岁健康成年母马身体4块分布全身、具有代表性的肌肉组织(长臂三头肌、夹肌、背最长肌、臀中肌)作为一个整体.胎儿期蒙古马肌纤维和成年期蒙古马肌纤维因存在差异各做为一组,试验进行3个生物学重复.首先对蒙古马骨骼肌肌肉样品进行免疫组化...  相似文献   

实验室计量认证（复审）——规范化管理方法的探讨     

杨凯  贾生军  王育杰  拉多杰  赵德娟  任珊 《青海农林科技》2012,(2):51-53

以实验室规范化管理工作中积累的一些经验为基础,对实验室计量认证（复审）工作进行了认真的探讨,确定建立符合实验室资质认定评审准则的质量体系,按体系文件进行检测工作全程序质量控制,以期探索出一种操作性强并具有广泛普及意义的“实验室规范化管理方法”。  相似文献   

A COMPARATIVE STUDY OF NUMERICAL CLASSIFICATION AND ORDINATION OF SOIL PROFILES IN A LOCALITY NEAR OXFORD     

H. E. CUANALO DE  LA C. R. WEBSTER 《European Journal of Soil Science》1970,21(2):340-352

Data for 85 soil profiles, located at random within 17 physiographic units in a 1,000 km² area near Oxford, and each characterized by 37 properties, were analysed by two sets of multivariate techniques. Principal component analysis yielded two components accounting for 44 per cent of the total variation, and the distribution of the profiles along these components is plotted as a scatter diagram (ordination). Similarity analysis and hierarchical grouping (classification) were performed using three different similarity indices, two different standardization techniques, and three different sorting strategies. Before analysis the profiles were classified by two pedologists, independently using their judgement. For the Brown Earths there was moderately good agreement between the results of the two prior classifications and between them and those produced by the several numerical procedures. The degree of clustering was slight and much less than imagined prior to analysis. For the Gleys there was no evident clustering. Different numerical procedures gave different results, as also did the two prior classifications. Pedologists faced with difficult classificatory decisions cannot look to any hierarchical clustering strategy as arbiter, though they should get guidance from a principal component plot.  相似文献   

Neoformed aragonite in clay soils on Keuper materials from east-central Spain   总被引：1，自引：0，他引：1  

H. A. LAYA  J. A. DE LA  PEÑA J. BENAYAS† 《European Journal of Soil Science》1992,43(3):401-407

Fibrous radial aragonite nodules occur in a toposequence developed in the Triassic (Keuper) in a subhumid mediterranean climate (Soria, Spain). Neoformation in situ of the nodules in these soils is proposed, on micromorphological and physico-chemical grounds, such as their fragility, the inclusion of material from the groundmass, their occurrence along cracks and their high Mg: Ca ratio.  相似文献   

Detoxication of drugs and other foreign compounds by liver microsomes     

BRODIE BB  AXELROD J  COOPER JR  GAUDETTE L  LA DU BN  MITOMA C  UDENFRIEND S 《Science (New York, N.Y.)》1955,121(3147):603-604

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甜菜总DNA不同提取方法的研究   总被引：2，自引：0，他引：2  

腊萍  罗淑萍  章建新  麻浩  刘娜  刘焕霞  赵图强 《新疆农业大学学报》2006,29(2):43-46

采用CTAB法、SDS法、改进CTAB法和改进SDS法提取甜菜总DNA,并对提取得到的DNA进行质量鉴定和PCR扩增效果检测.结果表明：用改进的CTAB法提取的甜菜总DNA OD260/OD280在1.8左右,DNA纯度好,产率高,CTAB法和改进的SDS法产率次之,SDS法最低.同时对改进的CTAB法的提取条件进行优化,即用5.0 mL细胞提取液,0.8 mL无水乙醇, 0.6倍体积的异丙醇,可从1.0 g甜菜叶片中提取到高质量高纯度的DNA 764 μg/g.  相似文献