板栗优良新品种‘黄棚’     

明桂冬  柳美忠  沈广宁  刘嘉芬  单公华  张美勇 《园艺学报》2005,32(3):564-564

 ‘黄棚’是20世纪90年代初从海拔300 m以上的山地栗园选出的实生变异优株。经多年高接鉴定和丰产栽培试验表明, 该品种早实, 丰产, 抗旱, 耐瘠薄, 品质优良, 为中早熟、适应范围广的炒食型板栗新品种。  相似文献   

彩色甜椒花药培养若干影响因子的研究   总被引：8，自引：0，他引：8  

蔡连华  雷建军  陈国菊  曾国平  曹必好 《中国瓜菜》2005,(4):16-19

利用硝酸银、ATP钠盐、NAA、KT进行了四因素三水平正交试验,材料为彩色甜椒品种7160的花药。结果表明,硝酸银是影响彩色甜椒花药培养胚状体诱导率的最主要因素,在其浓度为1.0mg/L时胚状体诱导率最高;ATP钠盐为次主要因素,在其浓度为100.0mg/L时效果最好。KT比NAA对胚状体诱导率的影响大;KT浓度1.0或1.5mg/L时较好。  相似文献   

H5N1亚型禽流感病毒NS1基因的原核表达及其ELISA检测方法的建立   总被引：4，自引：0，他引：4  

杨涛刘明  张云刘春国  杜绍范 《中国兽医科技》2005,35(8):585-589

采用RT-PCR技术扩增了禽流感病毒（AIV）A／Goose／HLJ／p46／2003（H5N1）的NSl基因，将其克隆于融合蛋白表达载体pMAL-c2X上，转化DH5α大肠埃希氏菌感受态细胞，经BamHⅠ和HindⅢ双酶切鉴定及序列分析，表明筛选到了重组质粒pc2X-NS1。SDS-PAGE电泳结果显示，重组质粒转化TB1大肠埃希氏菌后，经0．3mmol／L的IPTG诱导，融合蛋白MBP-NS1得到大量表达，融合蛋白以可溶形式存在，分子质量约为67ku。Western-blotting检测结果表明，融合蛋白MBP-NS1能够与H5N1亚型AIV活病毒感染康复鸭血清发生特异性反应，而不能够与H5N1亚型AIV灭活疫苗免疫鸭血清发生反应。试验初步建立了以纯化的融合蛋白MBP-NS1为包被抗原的间接ELISA检测方法，为AIV灭活疫苗免疫家禽与AIV感染家禽的鉴别诊断奠定了基础。  相似文献   

H7N2禽流感病毒分离株血凝素蛋白全基因的序列分析     

王传彬 孙明田克恭  金萍王宏伟  遇秀玲陈西钊 《中国兽医科技》2005,35(12):988-993

为进一步分析H7N2禽流感病毒（AIV）分离株血凝素（HA）基因的特性,参照已发表序列设计了1对引物,采用RT-PCR获得了1条约1.7 kb的DNA片段,测序后进行了同源性比较、HA基因系统发育进化树分析和氨基酸编码分析.结果表明,所测的2个分离株的HA基因全长1 664 bp,编码除信号肽以外的HA蛋白的全部544个氨基酸,其中包括HA1的323个氨基酸,HA2的221个氨基酸.2个分离株HA基因核苷酸序列的同源性为99.4%;与GenBank中AIV标准株A/Afri.Star./Eng-Q/983/79（H7N1）的同源性最高,分别为99.4%和99.0%;与美国A/Chicken/NewYork/13142-5/94（H7N2）株同源性很低（仅65.0%）,而与以色列、意大利H7N2 AIV的同源性较高（为96%～97%）;2个分离株在HA基因进化树中均处于H7亚型AIV的欧亚群系分支内.推导氨基酸的序列分析表明,其HA蛋白裂解位点的氨基酸序列为-GR-GLF-,仅包含1个碱性氨基酸（R-）残基,符合低致病力AIV的基因特征.  相似文献   

人为影响下古尔班通古特沙漠小尺度生态环境变化   总被引：1，自引：1，他引：1  

钱亦兵  张立运  雷加强  吴兆宁  张锐 《干旱区资源与环境》2003,17(6):82-88

随着沙漠中油气资源勘探开发、公路建设和重大工程相继实施 ,对生态环境的人为影响日益加深。本文以重大工程实验点为例 ,通过研究对比工程影响区内人工沙垄和自然沙垄的砂质新成土理化性状和植被群落特征 ,指出了沙漠生态环境在人为干扰下发生的一系列变化 ,以及受损生态环境在人们采取积极补救措施的情况下所具有的自然修复能力  相似文献   

Insulin induced LPL mRNA expression in THP-1 macrophage and acceleration of transferring the macrophage to foam cell     

ZHANG Xiao-gang  CHEN Yun-zhen  LEI Han  WANG Zhou-bi 《园艺学报》2003,19(6):832-836

AIM:To investigate the effect of insulin on ox-LDL transferring the THP-1 cells to foam cells and influencing the LPL mRNA expression in THP-1 cells.METHODS:THP-1 cells were incubated with 50 mg/Lox-LDL and insulin at concentrations of 10 mU/L, 100 mU/L, 1 000 mU/L and 10 000 mU/L, respectively. The expression of LPL mRNA in cells was detected by RT-PCR. Lipoprotein lipase of THP-1 cells was presented by no-specific lipase staining. THP-1 cells were stained with oil red O. Accumulation of total cholesterol (TC) in THP-1 cells was determined with oxidase assay.RESULTS:In 100 mU/L、1 000 mU/L、10 000 mU/L insulin groups, LPL mRNA expression increased 2 times, the average cell perilength was longer, the percentage of positive oil red O staining cells was significant higher, the content of cholesterol in THP-1 cells was higher than in ox-LDL control (P＜0.05).CONCLUSION:Insulin accelerates transferring of THP-1 cells to foam cell with exposed to ox-LDL because LPL mRNA expression increased in the cells.  相似文献   

不同地下水位植物蒸腾耗水特性研究   总被引：20，自引：6，他引：20  

赵明  郭志中  王耀琳  李爱德  张德魁  贾宝全 《干旱区研究》2003,20(4):286-291

在甘肃省民勤治沙站利用非称量测渗仪研究了梭梭、柠条等 10种沙区植物的蒸腾耗水特征。结果表明 ,各种植物 5a的总蒸腾量由大到小排列为 :沙枣、花棒、沙木蓼、柠条、梭梭、白皮沙拐枣 ;随植物年龄增长 ,蒸腾系数有上升的趋势 ,而蒸散系数有下降的趋势 ;在民勤沙区 6～ 9月是主要蒸腾季节 ,该时段的蒸腾量占全年蒸腾量的80 %～ 90 % ;植物的蒸腾量随年龄的增长而迅速增加 ,但增长幅度随物种不同而异。一般生长快的植物增长快 ,生长慢的植物增长慢。  相似文献   

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin     

LEI Xiao-yong  ZHANG Huan  HE Dong-mei 《园艺学报》2003,19(2):194-197

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.  相似文献   

Biological characteristics of long passaging rat mesenchymal stem cells     

LEI Jun-xia  LI Hao-wei  HUANG Chun-nong  ZHU Mei-ling  WEN Guan-mei  ZHANG Xiu-ming  LI Yan  LI Shu-nong 《园艺学报》2003,19(10):1325-1330

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.  相似文献   

Effect of tea-polyphenols on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells incubated with VLDL,LDL or ox-LDL     

ZHANG Xiao-gang  CHEN Yun-zhen  LEI Han  WANG Zhou-bi 《园艺学报》2003,19(10):1398-1402

AIM:To investigate the effect of tea-polyphenols (TP) on the activation of NF-κB and the expression of TGF-β₁ mRNA in THP-1 cells (a human acute monocytic leukemia cell line). METHODS:THP-1 cells were incubated with the different concentrations of TP, VLDL, LDL or ox-LDL. In the THP-1 cellls, the nuclear malposition rate of NF-κB was detected with immunohistochemistry technique, the positive index of the TGF-β₁ mRNA expression was detected by hybridization in situ, and accumulation of total cholesterol (TC) in cells incubated with 0.4-40 μg/L TP was determined with oxidase assay. RESULTS:The nuclear malposition rate of NF-κB, the positive index of the TGF-β₁ mRNA expression and TC in THP-1 cells incubated with 0.4-40 μg/L of TP were lower than those with 0 μg/L of TP in TP-V group, TP-L group and TP-O (P＜0.05). The differences of these markers in THP-1 cells incubated with more than 40 μg/L TP in TP-V group, TP-L group and TP-O were not statistically significant, compared with TP-C group (P＞0.05). CONCLUSION:TP inhibited the activation of NF-κB, the expression of TGF-β₁ mRNA and the foam cell formation in the mono-macrophage.  相似文献