不同厂家阿胶及动物皮胶中四种阴离子含量的测定     

刘雯  李峰  覃洁萍  冯旭 《湖北农业科学》2020,59(8):134-137

通过离子交换色谱法,建立不同厂家阿胶及动物皮胶中Cl~-、SO_4~(2-)、NO_3~-、PO_4~(3-)4种阴离子的测定方法。结果表明,4种阴离子分离效果良好,Cl~-、SO_4~(2-)、NO_3~-、PO_4~(3-)分别在0.000 922～0.023 1、0.001 09～0.027 3、0.000 167～0.004 18、0.000 314～0.007 86 mg/mL浓度范围内线性关系良好,平均加样回收率在98.7%～101.0%。该测定方法结果准确、灵敏、可靠,重现性较好,可用于阿胶及动物皮胶中4种阴离子含量的测定。  相似文献   

不同追肥量对油菜品种产量和经济效益的影响     

刘念  汤天泽  范其新  李迎春  蒙大庆  李芝凡  陈军  张体刚 《湖北农业科学》2020,59(18):71-73

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Effect of One-off Application of Poly Urea-Formaldehyde Fertilizer Under Reduced N Rate on Double Cropping Rice   总被引：2，自引：0，他引：2  

QiaoYi HUANG  Mu ZHANG  Xu HUANG  ShuanHu TANG  FaBao ZHANG  YuWan PANG  Qiong YI  Ping LI  HongTing FU 《中国农业科学》2018,51(20):3996-4006

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核桃黑斑病综合防治技术     

李新志 《陕西林业科技》2014,(6):80-81

通过对核桃黑斑病发病侵染规律及原因分析,采取加强水肥土管理,合理修剪,减少密蔽,改善通风透光条件;谨防蛀果害虫;越冬管理;药剂防治的综合措施防治,可有效控制核桃黑斑病的发生危害。  相似文献   

棉隆土壤消毒防除小麦田杂草的效果及对养分的影响   总被引：1，自引：0，他引：1  

朱文达  颜冬冬  李林  曹坳程 《湖北农业科学》2020,59(6):100-103

采用田间试验研究了98%棉隆微粒剂防除小麦田主要杂草的效果,及杂草防除后对氮、磷、钾及水分的影响。结果表明,施用98%棉隆微粒剂防除小麦田杂草效果显著,对菵草(Beckmannia syzigachne)、猪殃殃(Galium aparine L)、牛繁缕[Malachium aquaticum (L.) Fries]、大巢菜(Vicia sativa L.)均有良好防效,总鲜重防效达87.5%～99.5%,优于两种对照药剂。杂草防除后,显著降低了杂草对田间氮、磷、钾和水分的消耗,有效改善了田间的水肥条件。98%棉隆微粒剂处理的小麦田产量达2 970.01～3 123.77 kg/hm2,增产效果显著,产量较清水对照增加28.9%～35.6%,每公顷增收1 532.38～1 886.02元。  相似文献   

辽宁省公众生态文明意识调查分析     

李道宁  吕久俊  么旭阳 《辽宁林业科技》2016,(4):1-6

通过对辽宁省不同人群进行问卷调查,结合层次分析法、专家打分法,获取评价指标权重,对辽宁省公众生态文明意识现状进行分析。结果表明,辽宁省公众生态文明意识整体较好,其中认同度践行度知晓度,表明辽宁省公民对生态文明的了解、认识与行为之间存在一定的差异。根据调查结果,提出相应建议,为辽宁省生态文明建设提供科学依据。  相似文献   

Preparation of Atherosclerosis Model Using Guangxi Bama Mini-pig     

LI Yan-jun  SONG Shao-rui  GUO Ya-fen  LAN Gan-qiu  CHEN Bao-jian  YAN Xue-yu  WU Yan-jun  JIANG Qin-yang 《中国畜牧兽医》2015,42(7):1770-1776

The atherosclerosis (AS) model was prepared in Guangxi Bama mini-pig,and the atherosclerosis index (AI) was preliminarily identified in occurrence of AS to provide the basis for the preparation of related models.20 Guangxi Bama mini-pigs were randomly divided into control group and experimental group which were fed with high fat and cholesterol diet to prepare the AS model.Blood biochemical indexes were detected in the process of model preparation and the correlation between AI and the results of vascular slices was analyzed to initially draw up AI of AS.The results of vascular slices showed that the incidence rates of AS of Guangxi Bama mini-pigs in experimental group and control group were 20% and 0,respectively.The association analysis between the results of vascular slices and AI in the pathogenic Guangxi Bama mini-pigs preliminarily suggested that AI was above 3.8 and lasted for more than 3 months during the attack.  相似文献   

Development of Nucleic Acid Lateral Flow Immunoassay Detection Method for Foot-and-mouth Disease Virus     

GONG Zhen-li  JIANG Tao  QI Shu-yun  CHEN Guo-dong  LIU Yan-hong  LI Yong 《中国畜牧兽医》2015,42(9):2292-2297

In this article, through the combination of nucleic acid probes and immune chromatography, a simple, sensitive and specific detection system——nucleic acid lateral flow immunoassay (NALFIA) for amplifing foot-and-mouth disease virus (FMDV) 3D RT-PCR products was established.An ultrasensitive nucleic acid biosensor (NAB) based on streptavidin-labeled gold nanoparticles dual labels and lateral flow strip biosensor (LFSB) were used in this system.The biotinylated goat anti-rabbit IgG was marked to the NC membrane as the alleged strip and the anti-digoxin antibody was labeled to the NC membrane to capture the digoxin probe.After assemblying gold-labeled strip and detecting RT-PCR products, the detection limit of NALFIA was 0.3×10^-3 to 3×10^-3 μg/μL.The NALFIA was compared with agar gel electrophoresis analysis, the results showed that the sensitivity of NALFIA was higher than agar gel electrophoresis.There was an excellent agreement between the two methods.NALFIA was a method with high sensitive, low cost and short time.In conclusion, this method provided a good alternative to detect FMDV.  相似文献   

酸性土壤中决明属牧草高效根瘤菌的分离及筛选     

杨庆  王义祥  李欣欣  翁伯琦  廖红 《草地学报》2020,28(6):1519-1526

为提高豆科决明属（Chamaecrista spp.）牧草的结瘤能力和固氮效率，促进决明属牧草在酸性土壤中的应用及推广，本研究以圆叶决明（Chamaecrista rotundifolia）和羽叶决明（Chamaecrasta nictitans）为试验材料，通过分离、纯化、nodA基因鉴定等方法，从酸性土壤里决明属牧草的根瘤中分离到10株候选根瘤菌株系。16S rDNA测序及系统发育树分析表明，这10株根瘤菌均属于慢生型根瘤菌。回接试验表明，上述10株根瘤菌均能与圆叶决明共生，形成根瘤。与不接种的对照相比，其固氮酶活性为0.08~4.65 μmol·g^-1·h^-1，植株干重、SPAD值和氮含量分别提高了99.17%~372.14%，399.27%~789.05%，235.00%~1043.33%。其中，4株根瘤菌TXR2，TXN1，WYSR1，JYN6对圆叶决明氮营养贡献最大，并且TXN1与宿主共生形成的根瘤数目最多，而JYN6的固氮酶活性最高。说明本研究分离鉴定到4株决明高效根瘤菌，具有较好的应用前景。  相似文献   

Carbon storage and carbon density of forest vegetation in Luoyang,Western Henan Province     

WANG Yan-Fang  LIU Ling  LI Zhi-Chao  SHANGGUAN Zhou-Ping 《草业学报》2015,24(10):1

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