Evaluation of miltefosine for the treatment of dogs naturally infected with L. infantum (=L. chagasi) in Brazil     

Andrade HM  Toledo VP  Pinheiro MB  Guimarães TM  Oliveira NC  Castro JA  Silva RN  Amorim AC  Brandão RM  Yoko M  Silva AS  Dumont K  Ribeiro ML  Bartchewsky W  Monte SJ 《Veterinary parasitology》2011,181(2-4):83-90

Dogs naturally infected with Leishmania Infantum (=L. chagasi) were treated with miltefosine using different therapeutic regimens. The animals were evaluated for clinical evolution, biochemical parameters, parasite load (by real-time PCR), cytokine levels and humoral response. After treatment and during the following 24 months, there was progressive clinical improvement and complete recovery in 50% (7/14) of the treated animals. There was a decrease in the smear positivity of the bone marrow after treatment, and there was also a gradual and constant decrease in positive cultures at the end of the follow-up period. However, the PCR detection of parasite DNA remained positive. In general, all animals presented a significant increase in parasite load 6 months after treatment. The IFN-γ levels in all the groups tended to increase during follow-up period, regardless of the miltefosine dose administered. The IL-4 and IL-10 levels of the animals tended to decrease during follow-up, except after 300 days when only IL-10 increased. The serum antibodies identified antigens that ranged from 116 kDa to less than 29 kDa in the Western blot assay. Furthermore, 300 days after treatment, qualitative and quantitative differences in the antigen profiles were observed. Antigens of 97 and 46 kDa were the most intensely recognized. Higher levels of antigen-specific Leishmania IgG were detected before and 300 days after treatment in all groups. Taking together, the improvement in the clinical symptoms was not followed by parasitological clearance, suggesting that treatment with miltefosine is not recommended, especially in endemic areas like Brazil, where children are the major victims and dogs are involved in the maintenance of the parasite cycle.  相似文献   

Oocysts of Hepatozoon canis in Rhipicephalus (Boophilus) microplus collected from a naturally infected dog     

de Miranda RL  de Castro JR  Olegário MM  Beletti ME  Mundim AV  O'Dwyer LH  Eyal O  Talmi-Frank D  Cury MC  Baneth G 《Veterinary parasitology》2011,177(3-4):392-396

Canine hepatozoonosis is a tick-borne disease caused by protozoans of the genus Hepatozoon. Several tick species have been implicated as potential vectors. Therefore, extensive studies are needed to determine the 'natural' endemic cycle of this parasite. This paper presents the first report of the presence of Hepatozoon canis oocysts in Rhipicephalus (Boophilus) microplus collected from an infected dog.  相似文献   

Leishmania spp. in Didelphis albiventris and Micoureus paraguayanus (Didelphimorphia: Didelphidae) of Brazil     

Quintal AP  Ribeiro Ede S  Rodrigues FP  Rocha FS  Floeter-Winter LM  Nunes CM 《Veterinary parasitology》2011,176(2-3):112-119

Leishmaniasis is kept in nature by the participation of several animal species. This study evaluated the presence of Leishmania spp. in skin samples of free-ranging marsupials Micoureus paraguayanus (n=95) and Didelphis albiventris (n=191), captured in Morro do Diabo State Park and in sections of its surrounding forest, in the region of Pontal do Paranapanema, S?o Paulo State, Brazil. The samples were tested for the presence of kDNA of Leishmania spp. by polymerase chain reaction (PCR) and by real time PCR (qPCR). All samples from D. albiventris tested by PCR were negative for the presence of kDNA of Leishmania spp. However, when tested by qPCR, the positivity was 1.6%. A positivity of 7.4% by PCR and 11.6% by qPCR was observed for M. paraguayanus. Sixty-four per cent (9/14) of positive animals were limited to the same forest fragment. Presence of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis was detected in M. paraguayanus samples. While D. albiventris is the most studied marsupial species due to its urban habits, other marsupial species such as M. paraguayanus can be potential reservoirs of Leishmania spp. and should also be studied.  相似文献   

Acaricidal properties of the essential oil and precocene II obtained from Calea serrata (Asteraceae) on the cattle tick Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)     

Ribeiro VL  dos Santos JC  Martins JR  Schripsema J  Siqueira IR  von Poser GL  Apel MA 《Veterinary parasitology》2011,179(1-3):195-198

Calea serrata Less. (Asteraceae), an endemic species of south Brazil known as "quebra-tudo", is used in Afro-Brazilian religious rituals and in folk medicine for treating liver disorders. Phytochemical studies of the n-hexane extract of this plant demonstrated the presence of precocene II, a benzopyran derivative known for its insecticidal activity. The aim of this work was to isolate this benzopyran and determine the chemical composition of the essential oil of C. serrata and further to evaluate the acaricidal activity of the essential oil and precocene II against the larvae of Rhipicephalus (Boophilus) microplus. The LC(99.9) and LC(50) values obtained with the oil, which presents precocene II and sesquiterpenes, were 3.94 μL/mL and 0.28 μL/mL, respectively. For precocene II this values were 4.25mg/mL and 1.78 mg/mL, respectively. The results indicate a synergistic interaction between the components of the oil and precocene II.  相似文献   

Increasing consumption of ultra-processed foods and likely impact on human health: evidence from Brazil     

Monteiro CA  Levy RB  Claro RM  de Castro IR  Cannon G 《Public health nutrition》2011,14(1):5-13

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Squamous cell carcinoma of the footpad with systemic metastasis in a captive crowned solitary eagle (Buteogallus coronatus)     

Camila Maria da Silva ROCHA  Tais Meziara WILSON  Davi Emanuel Ribeiro de SOUSA  Alexandra Ariadne Bittencourt Gonalves PEREIRA  Andr Santos LEONARDO  Khesller Patrícia Olazia NAME  Mrcio Botelho de CASTRO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1627

Squamous cell carcinomas (SCC) are one of the most common tumors of the tegument that can have a misdiagnosis of chronic skin wounds. An adult captive crowned solitary eagle presented an indolent wound-like ulcer on the footpad and a fatal outcome. An infiltrating tumoral mass in the foot and multiple tumoral metastatic nodules in visceral organs were detected. The neoplasm was composed of atypical squamous cells with strong positivity for cytokeratin, “keratin pearl” structures, and marked invasion of tissues confirming a diagnosis of metastatic SCC. This might be the first report of an SSC with metastasis on the footpad in a captive Chaco eagle, which is one of the endangered species of birds of prey.  相似文献   

Effects of exogenous amylase on the in vitro digestion kinetics of whole-crop maize silages made from flint or dent grain type at different phenological stages grown in tropical condition     

Abias Santos Silva  Luiz Gustavo Ribeiro Pereira  Márcio dos Santos Pedreira  Fernanda Samarini Machado  Mariana Magalhães Campos  Cristina Simões Cortinhas  Tiago Sabella Acedo  Rafael Dantas dos Santos  João Paulo Pacheco Rodrigues  Rogério Martins Maurício  Thierry Ribeiro Tomich 《Journal of animal physiology and animal nutrition》2020,104(1):76-87

The effect of exogenous amylase on the in vitro rumen digestion kinetics of whole-crop maize silage made from dent (RB9004) or flint grain type (RB9308) was evaluated at different phenological stages: soft dough (SOD), early dent (EAD), ½ milkline (½M) and ¾ milkline (¾M). Forage was harvested from 70 to 110 days after sowing. Two rumen-cannulated cows receiving or not exogenous amylase (0.7 g/kg dry matter—DM, provided to achieve 396 kilo Novo units of amylase activity/kg of TMR DM) were used as donor of ruminal fluid. The in vitro gas production kinetics was evaluated according to a dual-pool logistic model. The chemical composition and gas production kinetics were affected by the hybrid and phenological stages. The flint hybrid had lower range for chemical analysis among physiological stages. Harvesting at ½M and ¾M improved DM content, bromatological composition and silage quality parameters compared to dent or flint types. Amylase (i) increased methane (CH₄) production and in vitro dry matter digestibility (IVDMD) in ½M stage, (ii) improved digestion kinetics by reducing lag time and increasing total gas production and fermentation rates of non-fibrous carbohydrates (NFC) and fibrous carbohydrates (FC), and (iii) increased extent and fermentation rate of NFC and increased fermentation rate of FC fraction in whole-crop maize silages produced from dent or flint types in all phenological stages. Harvesting between ½M and ¾M is the best phenological stage to improve chemical composition and silage quality parameters. Exogenous amylase showed improvements on fibre digestion of silages at ½M and ¾M phenological stages in both grain types of corn.  相似文献   

Combinations of cactus pear with different roughage sources on the production,chemical composition,and milk fatty acid profile of F1 Holstein/Zebu cows     

Soares  Camila  Rocha Júnior  Vicente Ribeiro  Monção  Flávio Pinto  Borges  Lucas Daniel Alcântara  Caldeira  Luciana Albuquerque  Costa  Natanael Mendes  Ruas  José Reinaldo Mendes  Rigueira  João Paulo Sampaio  da Cunha Siqueira Carvalho  Cinara  de Sales  Eleuza Clarete Junqueira  de Assis Pires  Daniel Ananias  Chamone  Julieta Maria Alencar 《Tropical animal health and production》2020,52(5):2567-2576

Tropical Animal Health and Production - The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals’ diets can...  相似文献   

Production,chemical composition,and economic viability of Minas Frescal cheese from buffaloes supplemented with açai seed     

de Oliveira  Wildiney Freire  de Lima  Elizanne Moura  de Freitas  Denise Ribeiro  dos Santos  Suely Sousa  Reis  Grazielle Carvalho  Gomes  Daiany Iris  Alves  Kaliandra Souza  Maciel  Raylon Pereira  Vargas  Julián Andrés Castillo  Mezzomo  Rafael 《Tropical animal health and production》2020,52(5):2379-2385

Tropical Animal Health and Production - We aimed to evaluate the chemical composition, production yield, and economic viability of Minas Frescal cheese produced with milk from buffaloes...  相似文献   

Sodium hydroxide or urea pretreatment of acerola (Malpighia emarginata) fruit residue increases dry matter degradability and reduces methane production in in vitro rumen fermentation     

Moura  Lais Micaelle Lopes  da Silva Pereira  Flávia Denise  de Lima  Patrícia Rodrigues  Nascimento  Júlio Cesar Silva  dos Santos de Oliveira  Andresca  de Souza Rodrigues  Rafael Torres  Ferreira  Clébio Pereira  Moraes  Salete Alves  Queiroz  Mário Adriano Ávila  Menezes  Daniel Ribeiro 《Tropical animal health and production》2020,52(5):2433-2441

Tropical Animal Health and Production - The aim of this study was to evaluate the effect of adding different concentrations of either urea or NaOH in dehydrated acerola (Malpighia emarginata) fruit...  相似文献