First molecular detection of Babesia vogeli in dogs from Brazil     

Passos LM  Geiger SM  Ribeiro MF  Pfister K  Zahler-Rinder M 《Veterinary parasitology》2005,127(1):81-85

The present work describes the detection and first molecular characterization of Babesia vogeli in dogs, naturally infected in Brazil and even in South America. Microscopic examination of Giemsa-stained peripheral blood smears collected from dogs originating from four different locations in Brazil revealed the presence of large Babesia merozoites and trophozoites (>2.5 microm). DNA was extracted from infected blood samples and PCR amplifications of the 18S rDNA were carried out. As a reference, DNA from an isolate of B. vogeli originated from Egypt was used. PCR products were purified and sequenced. The DNA sequences demonstrated 100% identity among the Brazilian isolates. Comparisons with the 18S rDNA sequence of the B. vogeli isolate from Egypt and with other B. vogeli sequences from Spain, France, Japan, Australia and South Africa confirmed the affiliation of all Brazilian isolates to the species B. vogeli.  相似文献   

Phylogeography of New World isolates of Anaplasma marginale based on major surface protein sequences   总被引：1，自引：0，他引：1  

de la Fuente J  Van Den Bussche RA  Garcia-Garcia JC  Rodríguez SD  García MA  Guglielmone AA  Mangold AJ  Friche Passos LM  Barbosa Ribeiro MF  Blouin EF  Kocan KM 《Veterinary microbiology》2002,88(3):275-285

Gene and protein sequences of major surface proteins (MSP) 1a and 4 of Anaplasma marginale (Rickettsiales: Anaplasmataceae) were used to infer phylogenetic relationships between New World isolates from Argentina, Brazil, Mexico and the United States. Seventeen isolates of A. marginale plus two outgroup taxa (A. centrale and A. ovis) were used for maximum-parsimony analysis of MSP4, while 20 isolates were used for phylogenetic analysis of MSP1a. msp4 analysis provided strong bootstrap support for a Latin American clade and, within this clade, support was detected for Mexican and South American clades. Isolates of A. marginale from the United States also grouped into two clades from the southern (isolates from Florida, Mississippi, and Virginia) and west-central (isolates from California, Idaho, Illinois, Oklahoma, and Texas) states. Although little phylogeographic resolution was detected within these higher clades, msp4 sequences appear to be a good genetic marker for inferring phylogeographic patterns of A. marginale isolates. In contrast to the phylogeographic resolution provided by msp4, MSP1a DNA and protein sequence were quite variable and did not provide phylogeographic resolution. Most variation in MSP1a sequences appeared unique to a given isolate and similar DNA sequence variation in msp1alpha was detected within isolates from Idaho and Florida and from Idaho and Argentina. The results of these studies demonstrated that msp4 provided phylogenetic information on the evolution of A. marginale isolates. In contrast MSP1a sequences appeared to be rapidly evolving and these sequences may provide phylogeographic information only when numerous isolate MSP1a sequences are analyzed from a geographic area.  相似文献   

Morphological aspects of the capybara stomach (Hydrochaeris hydrochaeris): gross and microscopic structure     

Moraes PT  Pacheco MR  de Souza WM  da Silva RA  Neto PB  Barreto CS  Ribeiro AA 《Anatomia, histologia, embryologia》2002,31(6):362-366

The digestive system of the capybara has been investigated because of its coprofagia habits, important for their absorptive activity. These species present differences in terms of gastrointestinal morphological characters when compared with other rodents. Macroscopiclly, the stomach of the capybara is constituted of the following parts: cardiac, pyloric, body, fundic and gastric diverticulum. It presents two curvatures, one big and another small. Externally, the presence of gastric bands (tenias) is observed. With regards to the volumetric view, the gastric capacity varies from 850 to 2010 ml, with an average of 1498.57 ml. So, the stomach of this animal can be classified as a simple stomach, in the format of a curved sack and similar to an inverted letter 'J'. The gastric mucous membrane presents a surface filled by numerous tortuous gastric folds and longitudinally distributed along all its extension. The mucous tunic also possesses recesses located among the successive gastric folds, which were denoted as gastric parts with numerous openings described as gastric pits. In the cardiac part, a glandular epithelium with cardiac glands is noticed containing a lot of parietal and mucous neck cells. The fundic part, body and gastric diverticulum contain proper gastric glands with main, parietal and mucous neck cells. Finally, the pyloric part has pyloric glands with two cellular types, mucous neck and parietal cells.  相似文献   

Effects of divergent selection for serum insulin-like growth factor-I concentration on performance, feed efficiency, and ultrasound measures of carcass composition traits in Angus bulls and heifers     

Lancaster PA  Carstens GE  Ribeiro FR  Davis ME  Lyons JG  Welsh TH 《Journal of animal science》2008,86(11):2862-2871

Angus bulls and heifers from lines divergently selected for serum IGF-I concentration were used to evaluate the effects of IGF-I selection line on growth performance and feed efficiency in 2 studies. In study 1, bulls (low line, n = 9; high line, n = 8; initial BW = 367.1 +/- 22.9 kg) and heifers (low line, n = 9; high line, n = 13; initial BW = 286.4 +/- 28.6 kg) were adapted to a roughage-based diet (ME = 1.95 Mcal/kg of DM) for 24 d and fed individually for 77 d by using Calan gate feeders. In study 2, bulls (low line, n = 15; high line, n = 12; initial BW = 297.5 +/- 34.4 kg) and heifers (low line, n = 9; high line, n = 20; initial BW = 256.0 +/- 25.1 kg) were adapted to a grain-based diet (ME = 2.85 Mcal/kg of DM) for 32 d and fed individually for 70 d by using Calan gate feeders. Blood samples were collected at weaning and at the start and end of each study, and serum IGF-I concentration was determined. Residual feed intake (RFI) was calculated, within study, as the residual from the linear regression of DMI on midtest BW(0.75), ADG, sex, sex by midtest BW(0.75) and sex by ADG. In study 1, calves from the low IGF-I selection line had similar initial and final BW and ADG, compared with calves from the high IGF-I selection line. In addition, DMI and feed conversion ratio were similar between IGF-I selection lines; however, calves from the low IGF-I selection line tended (P < 0.10) to have lesser RFI than calves from the high IGF-I selection line (-0.26 vs. 0.24 +/- 0.31 kg/d). In study 2, IGF-I selection line had no influence on performance or feed efficiency traits. However, there was a tendency (P = 0.15) for an IGF-I selection line x sex interaction for RFI. Bulls from the low IGF-I selection line had numerically lesser RFI than those from the high IGF-I selection line, whereas in heifers, the IGF-I selection line had no effect on RFI. In studies 1 and 2, weaning and initial IGF-I concentrations were not correlated with either feed conversion ratio or RFI. However, regression analysis revealed a sex x IGF-I concentration interaction for initial IGF-I concentration in study 1 and weaning IGF-I concentration in study 2 such that the regression coefficient was positive for bulls and negative for heifers. These data suggest that genetic selection for postweaning serum IGF-I concentration had a minimal effect on RFI in beef cattle.  相似文献   

The ecology of horse cyathostomin infective larvae (Nematoda-Cyathostominae) in tropical southeast Brazil     

Quinelato S  Couto MC  Ribeiro BC  Santos CN  de Souza LS  Dos Anjos DH  Sampaio IB  Rodrigues LM 《Veterinary parasitology》2008,153(1-2):100-107

Experimental studies about the recovery, survival and migration to pasture of cyathostomin infective larvae (L(3)) from fresh feces depositions were conducted from February 2005 to March 2007 in a tropical region of southeast Brazil. Grass and feces were collected weekly at 8 a.m., 1 and 5 p.m. and processed by the Baermann technique. Multivariate analysis (principal components method) showed the influence of time and environmental variables on the number of infective larvae recovered from the feces and pasture. In the rainy period (October-March), more infective larvae were recovered on the feces and grass apex. In contrast, in the dry period (April-September), the recovery was higher only on the grass base, as well as the L(3) survival on feces and grass. More larvae were recovered at 8 a.m., except from the grass apex, where the highest recovery was at 1 p.m. Few studies investigating the seasonal transmission of equine cyathostomin have been conducted in South American tropical climates. These results demonstrate that in tropical conditions L(3) are available on feces and pasture throughout the year. Knowledge of climatic influences on the development and survival of L(3) is crucial to designing integrated parasite control programs that provide effective protection while slowing the development of anthelmintic resistance.  相似文献   

Differentiation between canine cutaneous and visceral leishmaniasis by the detection of immunoglobulin G specific for Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi antigens using flow cytometry     

Santiago Mde A  Ribeiro FC  Mouta-Confort E  Nascimento LD  Schubach Ade O  Madeira Mde F  Bertho AL 《Veterinary parasitology》2008,154(3-4):341-346

Flow cytometry employing Leishmania (L.) chagasi (Lc) and L. (Viannia) braziliensis (Lb) antigen was used to establish the differential diagnosis between visceral (VL) and cutaneous leishmaniasis (CL) in dogs. Flow cytometry permitted the detection of Leishmania-specific immunoglobulin G in sera from 19 dogs: nine with CL and 10 with VL. A significant difference in the percentage of positive staining was observed in sera from dogs with CL between the homologous antigen (69% for Lb) and the heterologous antigen (42% for Lc). However, this difference was not significant in sera from dogs with VL (61% for Lb and 73% for Lc). No significant staining was observed in control sera (0.6% for Lb and 0.4% for Lc) consisting of samples from healthy dogs, or in the group with sporotrichosis (1.8% for Lb and 1.5% for Lc), a differential diagnosis of CL. The results suggest that flow cytometry might be useful for the differentiation between CL and VL in dogs, with practical applications in areas where the two infections overlap.  相似文献   

Brain monitoring in dogs using the cerebral state index during the induction of anaesthesia via target-controlled infusion of propofol   总被引：2，自引：1，他引：1  

Ribeiro LM  Ferreira DA  Bressan NM  Nunes CS  Amorim P  Antunes LM 《Research in veterinary science》2008,85(2):227-232

The aim of this study was to evaluate the correlation between the cerebral state index (CSI) and the estimated propofol plasma concentrations in dogs during induction of anaesthesia. Fifteen healthy dogs undergoing scheduled routine surgical procedures were enrolled in this study. Target controlled infusion (TCI) software, based on the pharmacokinetic model for propofol, was used to control the syringe pump and to estimate plasma propofol concentrations (PropCp) and the CSI values every five-seconds. Three electrodes placed in the centre of the forehead, on the left side of the forehead and on the left mastoid were used to collect the electroencephalographic (EEG) signal converted by the cerebral state monitor into the CSI. The cerebral electrical changes induced by increasing propofol concentrations appear to be detected by CSI monitoring in dogs. The negative correlation between CSI and PropCp demonstrates that the CSI could be used to assess electrical brain activity in dogs during the induction of anaesthesia with propofol.  相似文献   

Effects of dietary protein levels on activities of protease and expression of ingestion and protein digestion‐related genes in Nile tilapia juveniles     

Welliene M. Santos  Leandro S. Costa  Jose F. Lpez‐Olmeda  Natlia Cristina S. Costa  Fabio A. C. Santos  Pedro G. Gamarano  Walisson S. Silva  Priscila V. Rosa  Ronald K. Luz  Paula A. P. Ribeiro 《Aquaculture Research》2020,51(7):2973-2984

While some studies have investigated the effects of dietary protein on the activities of protease in Nile tilapia, little information exists about these enzymes and hormones regulating appetite at the molecular level. Therefore, we investigated the performance, protease activities and expression of genes related to protein intake and digestion in juvenile tilapia fed with diets containing different levels of crude protein (CP): 25.14, 31.12, 36.60 and 42.05%. The fish were fed to apparent satiation three times a day for 65 days. The animal performance and the retention rates of protein and energy linearly decreased with an increase in dietary CP content. A positive quadratic effect of CP on pepsinogen was observed, although the acid protease activity was not affected. Trypsinogen and trypsin activity in the intestine presented similar patterns, showing a positive quadratic response to dietary protein levels. A linear increase in intestinal chymotrypsinogen expression was observed, but the activity of chymotrypsin showed a positive quadratic response. In addition, the expression of cholecystokinin (cck) and peptide yy (pyy) increased linearly with increasing CP. Dietary protein modulates the activity of alkaline proteases as well as the expression of these protease genes and hormones involved in appetite regulation. These changes can help expand our understanding of feed intake and digestion control, thereby improving feed formulations for this species.  相似文献   

Effect of clove (Eugenia caryophyllus) and cinnamon (Cinnamomum zeylanicum) essential oils in Nile tilapia diets on performance,antioxidant power and lipid oxidation in fillets     

Cesar Sary  Fabiana Carbonera  Michele Cristina da Silva  Marina Oliveira  Vanessa Lewandowski  Humberto Todesco  Jesuí Vergílio Visentainer  Ivanor Nunes do Prado  Ricardo Pereira Ribeiro 《Aquaculture Research》2019,50(2):673-679

This study evaluated the addition (0.35 and 0.70 g/kg feed) of clove (EOcl) or cinnamon (EOci) essential oils to Nile tilapia feed for 45 days to assess animal performance, antioxidant capacity and lipid oxidation in fillets. The design was completely randomised with five treatments and three replicates. Antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC_FL), 2,2‐diphenyl‐1‐picrylhydrazylhydrate (DPPH), ferric reducing ability power (FRAP) and 2,2’‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulphonic acid) (ABTS) assays, and lipid oxidation was determined by the thiobarbituric acid‐reactive substances (TBARs) assay. Animal performance was not altered by adding the essential oils. The fillets treated with 0.35 g/kg of EOcl presented higher antioxidant capacity in the L‐ORAC_FL assay and a 40.3% reduction in lipid oxidation, whereas the EOci provided a pro‐oxidant effect relative to the control treatment. Based on these results, adding 0.35 g/kg of EOcl to the diet for 45 days before slaughter increased the antioxidant capacity and reduced the lipid oxidation of the fillets, contributing to product quality without altering animal performance.  相似文献   

Daily rhythms of cortisol and glucose and the influence of the light/dark cycle on anaesthesia in Nile tilapia (Oreochromis niloticus): Does the timing of anaesthetic administration affect the stress response?     

Leandro Santos Costa  Felipe Guedes de Araújo  Renan Rosa Paulino  Luciano Jos Pereira  Edgar Junio Damasceno Rodrigues  Paula Adriane Perez Ribeiro  Priscila Vieira Rosa 《Aquaculture Research》2019,50(9):2371-2379

Although daily variations in drug pharmacokinetics have been reported for a variety of teleost species, the influence of this daily variation on the cortisol response following anaesthesia remains poorly understood. To address this, two experiments were performed. The first experiment described the daily patterns of cortisol and glucose secretion in tilapia (Oreochromis niloticus). The second experiment investigated how the timing of anaesthetic administration (specifically at mid‐light [ML] or at mid‐dark [MD]) affects the induction and recovery times and plasma cortisol and glucose levels of juvenile Nile tilapia exposed to benzocaine, clove oil or tricaine methanesulphonate (MS‐222). The results revealed that the effect on the stress response associated with the moment when anaesthesia took place (ML or MD) varied according to the treatment (p < 0.05). Cortisol levels were significantly higher at ML for MS‐222 (ML = 116.23 ± 25.55; MD = 48.25 ± 22.33 ng/dl) (p < 0.05) and clove oil (ML 59.73 ± 14.27; MD 38.26 ± 12.07 ng/dl) (p < 0.05), whereas no significant differences were found between ML and MD cortisol levels for the control treatment (ML = 72.91 ± 18.42; MD = 64.80 ± 10.68 ng/dl) (p > 0.05) or in the benzocaine‐treated group (ML = 38.7 ± 4.90; MD = 38.60 ± 3.69 ng/dl) (p > 0.05). The highest plasma cortisol level in ML was found in the MS‐222‐treated group. All the tested anaesthetics had similar cortisol levels at MD (p > 0.05).  相似文献