Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events     

WU Yu-hua  ;ZHANG Li  ;WU Gang  ;NIE Shu-jing  ;LU Chang-ming 《中国农业科学(英文版)》2014,(9):1865-1876

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.  相似文献   

GC-MS/MS分析中药六方藤和山苦荬中46种禁限用农药残留量   总被引：1，自引：0，他引：1  

唐明华  蒋永宁  徐梦  卢桂健  梁爽 《湖北农业科学》2020,59(21)

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草原生态奖补政策实施效应——基于政治社会学视角的实证分析     

路冠军  刘永功 《干旱区资源与环境》2015,(7):29-32

草原生态奖补政策有助于保护草原生态,促进畜牧业的发展转型,有利于民族地区的和谐稳定。生态奖补政策的实施对牧民生计、基层社会治理等方面也产生了一些负面影响。未来的政策实践过程中,需要建立政策评估反馈机制,不断提高生态奖补标准,完善奖补资金发放办法,着力调动地方政府积极性,力求实现奖补手段的多样化,不断提高政策的法制化、系统化水平。  相似文献   

Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells     

DUAN An-qin  PANG Chun-ying  ZHU Peng  LU Xing-rong  DENG Ting-xian  LIANG Xian-wei 《中国畜牧兽医》2016,43(10):2661-2665

Buffalo mammary epithelial cell,cumulus cell and fibroblasts were transfected by adenovirus vectors and compared their transfection efficiency.293 cells were transfected with pBHGloxdelE13cre and pDC316-eGFP by liposome,the virus was collected and titer was detected.Buffalo mammary epithelial cell,cumulus cell and fibroblasts were exposed to different multiplicity of infection (MOI) of adenovirus vectors.After 72 h,the cells were observed with inverted fluorescence microscope,and transfection efficiency was calculated.When the MOI was 25,50,100,200 and 400,the transfection efficiency of fibroblasts were 0.7%,7.0%,9.0%,12.5% and 34.0%,the transfection efficiency of cumulus cells were 42.5%,55.3%,57.4%,76.0% and 80.0%,the transfection efficiency of mammary epithelial cells were 88.7%,100%,100%,100% and 100%.The results showed that the transfection efficiency of mammary epithelial cell was the best,followed by cumulus cell,and fibroblast was poor.  相似文献   

Effects of Different Nitrogen Treatments on N use Efficiency and Grain Yield of Mechanically-transplanted Hybrid Rice     

Chang YE  Ke LIU  Shaowen LIU  Jian LU  Mengtao GAO  Bilin LU  Yunbo ZHANG 《农业科学与技术》2017,18(2)

In order to investigate effects of topdressing on grain yield and N use efficiency of mechanically-transplanted hybrid rice,this experiment was conducted with 2-line hybrid rice cultivars,Quanliangyou 1 and Quanliangyou 681 with 3 application levels of N fertilizer,No,N1,N2,respectively.The results showed that there were no significant differences in grain yields of the 2 varieties between treatments N1 and N2.It was indicated that N is not the factor which could limit grain yield within limited application levels of N fertilizer.High N fertilizer treatment could contribute to the formation of effective panicles and spikelets per panicle,but it could also lead to the decline in grain filling rate.It was suggested that lowering the amount of Nfertilizer could facilitate improvement of N-use efficiency.  相似文献   

Cloning and Sequence Analysis of Buffalo Keap1 Gene and Investigation of Its Expression Pattern in Different Tissues     

ZHAO Xin  RUAN Zi-yun  GUO Zhen-wei  ZHOU Wen-ting  QIN Xi-ling  NIU Xiang-li  LU Feng-hua  SHI De-shun 《中国畜牧兽医》2016,43(8):1975-1982

In this study,the CDS sequence of buffalo Keap1 gene was cloned and analyzed,then its expression pattern in different tissues was also investigated.A pair of primers of buffalo Keap1 gene was designed based on the nucleotide sequence of Bos taurus Keap1 gene from GenBank,and then the buffalo Keap1 gene was amplified.Using the bioinformation techniques,the gene sequence and the protein structure were analyzed.The expression level of Keap1 gene in different tissues were detected with Real-time quantitative PCR.The results showed that the length of buffalo Keap1 gene coding sequence was 1 875 bp and encoded 624 amino acids.The multiple sequence alignment results showed that buffalo Keap1 gene shared 99%,96%,92% and 90% of similar nucleotide sequence with that of Bos taurus,Ovis aries,Sus scrofa and Homo sapiens,respectively.And the phyogenetic tree also showed the conservatism between several different species.The second structure of buffalo Keap1 protein was predicted as 24 alpha regions,40 beta regions,38 turn regions and 27 coil regions.In addition,the results of Real-time quantitative PCR showed that Keap1 mRNA exists in all seven tissues,but the most abundant expression was in heart and the minimal expression was in liver and spleen.The results provided an foundation for further study of Keap1-Nrf2-ARE signal pathway,for enhancing the ability of antioxidant of buffalo embryo in vitro culture.  相似文献   

施硒和6-BA对葡萄叶片衰老与活性氧代谢的影响   总被引：1，自引：0，他引：1  

王帅  王海波  王孝娣  史祥宾  王宝亮  郑晓翠  刘凤之 《果树学报》2015,(2):206-214

【目的】明确氨基酸硒和6-BA对葡萄叶片衰老与活性氧代谢的影响,为延缓叶片衰老技术的提出提供理论依据。【方法】以设施延迟栽培条件下叶片衰老速度不同的‘意大利’和‘无核白鸡心’2个葡萄品种为试材,分别进行叶面喷施氨基酸硒和6-BA处理,以喷施等量清水作为对照,研究施硒及6-BA对叶片衰老期间各功能叶片的叶绿素含量、净光合速率(Pn)、丙二醛(MDA)含量和超氧阴离子自由基(O2·)、过氧化氢(H2O2)含量及过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)抗氧化酶活性的影响。【结果】与对照相比,外源氨基酸硒和6-BA处理显著延缓了叶片的叶绿素含量和净光合速率(Pn)的下降,提高了过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性,从而减慢了丙二醛(MDA)、超氧阴离子自由基(O2·)、过氧化氢(H2O2)的上升速率,延缓了叶片衰老。2个品种间比较,‘意大利’叶片衰老缓慢,在生育后期仍能维持较高的抗氧化酶活性。【结论】氨基酸硒和6-BA处理可有效提高叶片的抗衰老能力,延长叶片的功能期。  相似文献   

江西省新型农业社会化服务体系的特点与运行模式     

薛刚  卢琨  李玉萍 《热带作物学报》2016,(3):66-68

实地调研江西省新型农业社会化服务体系建设现状，对江西省目前的新型农业社会化服务体系的特点与运行模式进行分析，针对当地在服务体系构建过程中出现的问题，提出新时期的发展建议。  相似文献   

不同药剂组合对马铃薯晚疫病的防治效果     

韩小女  展康  蔡永占  何彩花  吕庆长  杨亚琼 《中国马铃薯》2020,(1):46-52

马铃薯晚疫病是马铃薯生产中的重要病害,是影响马铃薯产量和品质的重要因素之一,在云南春作马铃薯上普遍发生,且危害较重,给当地马铃薯产业带来了巨大的经济损失。为减轻马铃薯晚疫病对马铃薯生产造成的损失,试验研究了11种药剂7种药剂组合(包衣剂+保护剂+治疗剂)通过播种期种薯包衣,现蕾期叶面喷施一次保护剂,发病初期和发病中期叶面各喷一次治疗剂的方法防治马铃薯晚疫病。结果表明,药剂组合种薯包衣+甲霜·锰锌+氟吡菌胺·霜霉威的防治效果最好,防治效果达到60.99%,增产率176.28%,增效率152.27%;其次是种薯包衣+霜脲·锰锌+氟吡菌胺·霜霉威组合,防治效果达到60.15%,增产率173.20%,增效率150.55%。这2个药剂组合对马铃薯晚疫病均有极显著的防治效果,增产增效明显。  相似文献   

鹌鹑ESR1基因的外显子8多态性与早期生长性能的关联分析     

樊红灯  白俊艳  付学言  曹恒  杨帅  庞有志  时坤鹏  卢小宁  马永康  陈梦柯  董智豪  安肖凯  常世增  侯伟晓  巩慧荣 《黑龙江畜牧兽医》2020,(2)

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