Determination of log moisture content using early-time ground penetrating radar signal     

Guillaume Hans  David Redman  Brigitte Leblon  Joseph Nader  Armand La Rocque 《Wood material science & engineering》2015,10(1):112-129

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Abies sibirica in the Ural region is attacked by the S type of Heterobasidion annosum     

K. Korhonen  N. I. Fedorov  N. La Porta  N. P. Kovbasa 《Forest Pathology》1997,27(5):273-281

Specimens of Heterobasidion annosum were collected in seven mixed stands of Abies sibirica and Picea abies in Yekaterinburg and Perm regions on the western side of the Ural Mountains, Russia. Pure cultures were isolated from 60 collections and identified in mating tests. Only the S intersterility group was found, attacking both fir and spruce. Based on the appearance of clamp connections in matings, the compatibility of 12 homokaryons from the Ural area with 14 S group homokaryons from western Europe was 100%, with 13 F group homokaryons from southern Europe 76% and 9 P group homokaryons from western Europe 3%. The results show that A. sibirica, in contrast to A. alba, is very susceptible to the S type H. annosum.  相似文献   

Analysis of S‐allele genetic diversity in Sicilian almond germplasm comparing different molecular methods          下载免费PDF全文

Sergio Currò  Stefano La Malfa  Gaetano Distefano  Guiyou Long  Francesco Sottile  Alessandra Gentile 《Plant Breeding》2015,134(6):713-718

Italian almond germplasm is characterized by a wide diversity in several growing areas among which Sicily is one of the most important. Analysis with consensus and specific primers and DNA sequencing was performed to investigate S‐RNase genetic diversity and to elucidate the homology rate within a genetic pool of 27 Italian accessions. Interestingly, some of the self‐compatible cultivars did not show the presence of S_f allele. Amplicons from consensus and allele‐specific PCR primers revealed a high level of variability. Sequencing of all the S‐RNase amplicons derived from consensus primers allowed the identification of two new S‐RNase alleles (S₅₁ and S₅₂). Surprisingly, despite the AA replacement mutation, S₅₁ did not exhibit any change of its S‐RNase function. Additionally, several mutations, with no effect on amino acid composition, were detected in the intron and/or in the ORF of four known alleles (S_g, S₁₀, S₃₁ and S₃₅). Genetic variation, regarding point mutations and only detected by sequencing, was revealed among 11 of 27 tested cultivars. The new sources of variability might have an interest for product traceability.  相似文献   

Validating the Use of 137Cs Measurements to Derive the Slope Component of the Sediment Budget of a Small Rangeland Catchment in Southern Italy     

Paolo Porto  Desmond E. Walling  Carmelo La Spada  Giovanni Callegari 《Land Degradation \u0026amp; Development》2016,27(3):798-810

The sediment budget is a key concept and tool for characterizing the mobilization, transfer and storage of fine sediment within a catchment. Caesium‐137 measurements can provide valuable information on gross and net erosion rates associated with sheet and rill erosion that can be used to establish the slope component of a catchment sediment budget. However, there is a need to validate the use of ¹³⁷Cs measurements for this purpose, because their reliability has sometimes been questioned. The study reported focuses on a small (3·04 ha) steepland (mean slope 37%) catchment in Southern Italy. It exploits the availability of information on the medium‐term sediment output from the catchment provided by the construction of a reservoir at its outlet in 1978 and the existence of estimates of soil redistribution rates derived from ¹³⁷Cs measurements made on 68 replicate soil cores collected from the slopes of a substantial proportion of the catchment in 2001, to validate the use of ¹³⁷Cs measurements to construct the slope component of the catchment sediment budget. An additional 50 replicate soil cores were collected from the catchment slopes for ¹³⁷Cs analysis, to complement the data already available. Nine cores collected from the area occupied by the reservoir were used to estimate the mean annual sediment input to the reservoir. In the absence of evidence that the poorly developed channel system in the catchment was either a significant sediment source or sink, it was possible to directly compare the estimate of net soil loss from the catchment slopes (7·33 Mg ha⁻¹ y⁻¹) with the estimate of sediment output from the catchment provided by the reservoir deposits (7·52 Mg ha⁻¹ y⁻¹). Taking account of the uncertainties involved, the close agreement of the two values is seen as providing a convincing validation of the use of ¹³⁷Cs measurements to both estimate soil redistribution rates and as a basis for constructing the slope component of the sediment budget of a small catchment. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

Broccoli ( Brassica oleracea var. italica) sprouts and extracts rich in glucosinolates and isothiocyanates affect cholesterol metabolism and genes involved in lipid homeostasis in hamsters     

Rodríguez-Cantú LN  Gutiérrez-Uribe JA  Arriola-Vucovich J  Díaz-De La Garza RI  Fahey JW  Serna-Saldivar SO 《Journal of agricultural and food chemistry》2011,59(4):1095-1103

This study investigated the effects of broccoli sprouts (BS) on sterol and lipid homeostasis in Syrian hamsters with dietary-induced hypercholesterolemia. Treatments included freeze-dried BS containing 2 or 20 μmol of glucoraphanine (BSX, BS10X), glucoraphanine-rich BS extract (GRE), sulforaphane-rich BS extract (SFE), and simvastatin. Each experimental diet was offered to eight animals (male and female) for 7 weeks. Hepatic cholesterol was reduced by BS10X and SFE treatments in all animals. This correlated with a down-regulation of gene expression of sterol regulatory element-binding proteins (SREBP-1 and -2) and fatty acid synthase (FAS) caused by GRE and SFE diets. BS10X caused changes in gene expression in a gender-specific manner; additionally, it increased coprostanol excretion in females. With the same concentration of glucoraphanin, consumption of broccoli sprouts (BS10X) had more marked effects on cholesterol homeostasis than GRE; this finding reinforces the importance of the matrix effects on the bioactivity of functional ingredients.  相似文献   

Epidural analgesia with morphine or buprenorphine in ponies with lipopolysaccharide (LPS)-induced carpal synovitis     

Freitas GC  Carregaro AB  Gehrcke MI  De La Côrte FD  Lara VM  Pozzobon R  Brass KE 《Canadian journal of veterinary research》2011,75(2):141-146

This study evaluated the analgesia effects of the epidural administration of 0.1 mg/kg bodyweight (BW) of morphine or 5 μg/kg BW of buprenorphine in ponies with radiocarpal joint synovitis. Six ponies were submitted to 3 epidural treatments: the control group (C) received 0.15 mL/kg BW of a 0.9% sodium chloride (NaCl) solution; group M was administered 0.1 mg/kg BW of morphine; and group B was administered 5 μg/kg BW of buprenorphine, both diluted in 0.9% NaCl to a total volume of 0.15 mL/kg BW administered epidurally at 10 s/mL. The synovitis model was induced by injecting 0.5 ng of lipopolysaccharide (LPS) in the left or right radiocarpal joint. An epidural catheter was later introduced in the lumbosacral space and advanced up to the thoracolumbar level. The treatment started 6 h after synovitis induction. Lameness, maximum angle of carpal flexion, heart rate, systolic arterial pressure, respiratory rate, temperature, and intestinal motility were evaluated before LPS injection (baseline), 6 h after LPS injection (time 0), and 0.5, 1, 2, 4, 6, 8, 10, 12, 16, 20, and 24 h after treatments. Although the model of synovitis produced clear clinical signs of inflammation, the lameness scores in group C were different from the baseline for only up to 12 h. Both morphine and buprenorphine showed a reduction in the degree of lameness starting at 0.5 and 6 h, respectively. Reduced intestinal motility was observed at 0.5 h in group M and at 0.5 to 1 h in group B. Epidural morphine was a more effective analgesic that lasted for more than 12 h and without side effects. It was concluded that morphine would be a valuable analgesic option to alleviate joint pain in the thoracic limbs in ponies.  相似文献   

Epidermal growth factor and active caspase-3 expression in the levator ani muscle of dogs with and without perineal hernia     

Pérez-Gutiérrez JF  Argüelles JC  Iglesias-Núñez M  Oliveira KS  De La Muela MS 《The Journal of small animal practice》2011,52(7):365-370

Objectives : To perform a histological and immunohistochemical study of epidermal growth factor, transforming growth factor‐alpha and their receptor, as well as the apoptotic signal active caspase‐3 in the levator ani muscle of dogs with and without perineal hernia. Methods : Biopsy specimens of the levator ani muscle were obtained from 25 dogs with perineal hernia and 4 non‐affected dogs and were processed for Masson and immunohistochemical staining. Results : The affected dogs exhibited myopathological features, internalised nuclei, destruction and abnormal size of muscle fibres, which were replaced by collagen. The immunohistochemical study revealed active caspase‐3, epidermal growth factor, transforming growth factor‐alpha and epidermal growth factor receptor in the levator ani. Compared to the healthy muscle, transforming growth factor‐alpha staining intensity was lower in the affected muscle, whereas epidermal growth factor receptor and active caspase‐3 staining were higher. Clinical Significance : Pelvic diaphragm muscle weakening is the leading cause of perineal hernia in the dog. Survival and death signals expressed in these muscles may contribute to the pathogenesis of this disease. This study reports epidermal growth factor, transforming growth factor‐alpha and epidermal growth factor receptor immunohistochemical expression in the skeletal muscle and suggests that perineal hernia in the dog is accompanied by levator ani muscle atrophy, increased expression of epidermal growth factor receptor, caspase‐3 activation, and decreased expression of transforming growth factor‐alpha.  相似文献   

Where do we go from here? Dispersal simulations shed light on the role of landscape structure in determining animal redistribution after reintroduction     

V. La Morgia  Elisa Malenotti  Guido Badino  Francesca Bona 《Landscape Ecology》2011,26(7):969-981

Reintroduction projects represent viable options for animal conservation. They allow the establishment of new local populations and may contribute to recreating functional networks within a metapopulation. In the latter case, landscape connectivity may be a major determinant of the phase of spread of the reintroduced populations. Here, we deal with an example of a red deer (Cervus elaphus) translocation planned to enable the connection among existing isolated populations of the species in the Italian Alps. Our aim was to assess whether the analysis of landscape suitability and the simulation of dispersal of released individuals could shed light on the actual process of population spread. For these purposes, we adopted a modelling approach using radiotracking data to develop a habitat suitability map. On the basis of this map, we simulated the dispersal of the animals after release and we then compared the simulation results with the outcome of null models and with the observed population redistribution. The results suggest that the spread of the subpopulation was easier north-westward than southward. Taking into account landscape suitability, our simulations produced a reliable estimate of the ease of colonization of the valleys neighbouring the release-site and they allowed the identification and validation of a potential pathway for animal dispersal. The suitability model based on the monitoring of individuals in the earliest phase of establishment shed light on the spread of the population and on its potential connections with other deer subpopulations.  相似文献   

Genetic parameters for milk,growth, and reproductive traits in Guzerá cattle under tropical conditions     

Brito  Laís Costa  Peixoto  Maria Gabriela Campolina Diniz  Carrara  Eula Regina  Fonseca e Silva  Fabyano  Ventura  Henrique Torres  Bruneli  Frank Angelo Tomita  Lopes  Paulo Sávio 《Tropical animal health and production》2020,52(5):2251-2257

Tropical Animal Health and Production - This study aimed to estimate the genetic parameters of milk (305-day milk yield (MY305)), growth (weaning weight (WW), yearling weight (YW), and weight at...  相似文献   

Genome-wide scan for runs of homozygosity in the composite Montana Tropical® beef cattle     

Elisa Peripolli  Nedenia Bonvino Stafuzza  Sabrina Thaise Amorim  Marcos Vinícius Antunes de Lemos  Laís Grigoletto  Sabrina Kluska  José Bento Sterman Ferraz  Joanir Pereira Eler  Elisângela Chicaroni Mattos  Fernando Baldi 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2020,137(2):155-165

The aim of this study was to assess the distribution of runs of homozygosity (ROH) and autozygosity islands in the composite Montana Tropical^® beef cattle to explore hotspot regions which could better characterize the different biological types within the composite breed. Montana animals (n = 1,436) were genotyped with the GGP-LD BeadChip (~30,000 markers). ROH was identified in every individual using the plink v1.90 software. Medium and long ROH prevailed in the genome, which accounted for approximately 74% of all ROH detected. On an average, 2.0% of the genome was within ROH, agreeing with the pedigree-based inbreeding coefficient. The Montana cattle with a higher proportion of productive breed types showed the highest number of autozygosity islands (n = 17), followed by those with a higher proportion of breeds adapted to tropical environments (n = 15). Enriched terms (p < .05) associated with the immune and inflammatory response, homeostasis, reproduction, mineral absorption, and lipid metabolism were described within the autozygosity islands. In this regard, over-represented GO terms and KEGG pathways described in this population may play a key role in providing information to explore the genetic and biological mechanisms together with the genomic regions underlying each biological type that favoured their optimal performance ability in tropical and subtropical regions.  相似文献