牛GDF-9基因的克隆与组织表达的分析     

田蕾  刘永斌  王峰  田春英  刘美霞  达赖  祁云霞  董文杰  荣威恒  孙洪深 《华北农学报》2007,22(4):67-70

为了检测牛GDF-9基因mRNA在怀孕期牛生殖系统的表达情况,为GDF-9基因在雌性动物繁殖上的研究提供一定的理论依据。本试验根据GenBank中报道的牛GDF-9基因序列设计引物,从牛卵巢、输卵管、子宫、肾脏中提取RNA,采用RT-PCR技术进行cDNA扩增,扩增产物与载体pGM-T连接,转化大肠杆菌DH5α,筛选阳性克隆,测序。结果表明,已成功克隆的阳性重组子经测序鉴定其片段大小与预期大小完全一致,为264 bp。序列同源性分析比较表明,该cDNA及其推导的氨基酸序列与绵羊GDF-9cDNA和氨基酸序列同源性分别为97%和94%。以-βactin为内参照物,采用RT-PCR技术进行半定量分析,通过检测比较牛GDF-9基因在卵巢、输卵管、子宫、肾脏组织中mRNA的表达情况。结果表明,牛GDF-9基因在卵巢中表达量最高,在输卵管、子宫、肾脏中均有表达,但表达量不高。  相似文献   

高产优质番薯品种比较试验     

赖世膺 《种子世界》2001,(6):19-20

1试验设计与经过 该试验设立在泰顺县万排乡王家洋村徐继桂承包田上.海拔为500m,前作紫云英,土质属沙性红黄壤土,肥力中上,肥力分布均匀一致,光温一致.密度:1.17m×0.36m,每小区栽插30株,折1 587株/667m2.小区面积12.64m2.参试品种5个,即:浙8678、浙1257、浙6025、浙50、瑞薯1号.以当地常规品种瑞薯1号作对照(ck),设5个小区,3次重复.采用顺序间比法排列,两头设保护行.基肥:用焦泥灰500kg/667m2,腐熟栏肥500kg/667m2,复混肥20kg/667m2.栽后用人粪150kg/667m2对水"滴口",插后22d铲草、松土,结合追肥用人肥150kg/667m2、尿素5kg/667m2对水种穴.全生育期轻提蔓2次.  相似文献   

Developments of Cotton Cultivation in Sinkiang     

Chuen-Yan David Lai 《Asia Pacific viewpoint》1976,17(2):159-166

  相似文献   

回归最优设计法在水培白菜肥料效应中的应用          下载免费PDF全文

王忠全  孙莉娜  赖冬梅  魏佩如  林文生  罗盛海 《中国农学通报》2007,23(2):138-138

利用“416-A”回归最优设计研究Ca(NO3)2?4H2O、KNO3、NH4H2PO4、MgSO4?7H2O对水培白菜的产量效应。数学模拟建立了四种肥料与白菜产量的回归方程：y=97.808-4.337x1+3.445x2-2.690x3+8.299x4-6.894x1x2+17.331x1x3-7.389x1x4-3.191x2x3+6.364x2x4+0.063x3x4-9.629x12-17.150x22-17.915x32-24.066x42。经检验回归关系显著，并对回归系数进行了检验。从回归方程可得：MgSO4?7H2O对产量以直接作用为主，其次为Ca(NO3)2?4H2O、KNO3和NH4H2PO4。Ca(NO3)2?4H2O与其它三种肥料均有交互作用，其中与NH4H2PO4交互作用达极显著水平。从产量效应回归关系优选出配方浓度为：Ca(NO3)2?4H2O：971mg/L；KNO3：931mg/L；NH4H2PO4：204mg/L；MgSO4?7H2O：581mg/L。采用回归最优设计结合水培研究肥料效应精确可行。  相似文献   

不同熟化度红壤及红壤性水稻土的腐殖质组成及其特性   总被引：6，自引：2，他引：6  

林明海  赖庆旺 《土壤学报》1982,19(3):237-247

土壤腐殖质是评价红壤肥力的重要因素^[2],根据前人研究^[8,3,1]:一般土壤腐殖质的含量及组成受土壤地带性的影响。胡敏酸与富啡酸的比值自南向北逐渐递增,红壤胡敏酸与富啡酸的比值小于1。本文着重研究同一地区自然成土因素类同的情况下,红壤通过水旱耕作和培肥,土壤腐殖质的组成及特性的变化。  相似文献   

Availability of Heavy Metals for Brassica Chinensis Grown in an Acidic Loamy Soil Amended with a Domestic and an Industrial Sewage Sludge   总被引：1，自引：0，他引：1  

Wong  J. W. C.  Lai  K. M.  Su  D. S.  Fang  M. 《Water, air, and soil pollution》2001,128(3-4):339-353

The use of sewage sludge on agriculture provides an alternativefor sewage sludge disposal. Therefore, it was the aim of thepresent study to evaluate the feasibility of using a domestic(Tai Po sludge) and an industrial (Yuen Long sludge) sewagesludge produced in Hong Kong for the growth of vegetable crops.The acidic loamy soil with or without lime treatment was amendedseparately with each sludge at application rates of 0, 5, 10, 25and 50% (v/v) for the growth of a common local vegetable crop,Brassica chinensis. The plant available metal contents, asindicated by the DTPA extraction, increased with an increase insludge amendment, but decreased with lime amendment at eachsludge application rate due to the reduced metal availabilityat a higher pH. Sludge amendment enhanced the dry weight yieldof B. chinensis and the increase was more obvious for thesoil with lime treatment. The industrial sludge caused a loweryield than that of the domestic sludge amendment and asignificant reduction in yield at high application rates of YuenLong sludge was also noted. Tissue heavy metal contents, exceptfor Fe, increased as the sludge amendment rate increased whileplant grown in Yuen Long sludge amended soil contained higher Crand Zn contents at each sludge application rate. Liming the soilreduced the heavy metal contents in the plant tissues, exceptfor Fe, which were all below the allowable levels for vegetablecrops. The present experiment demonstrates that liming wasimportant in facilitating the growth of B. chinensis in sludge amended soil. The optimal sludge amendment rate for thesoil with lime amendment was 25% Tai Po sludge and 10% YuenLong sludge, while for the soil without lime amendment was 10% and5%, respectively.  相似文献   

洪雅县退耕竹林碳储量时空格局   总被引：2，自引：0，他引：2  

唐骄萍  李贤伟  赖元长  冯帅  王鹏  黄从德 《林业科学》2011,47(10)

建立退耕还林重点示范县洪雅县竹林碳储量多元线性回归遥感模型,基于碳储量遥感模型估算出四川省洪雅县退耕还林以前(1994年)、退耕还林后第4年(2004年)和退耕还林后第7年(2007年)的竹林碳储量,利用地理信息系统软件对竹林碳储量的时空格局变化进行定量分析。结果表明:13年间(1994—2007年)洪雅县竹林碳储量和面积均呈现逐年上升趋势,前期(1994—2004年)增速大于后期(2004—2007年);竹林碳储量和面积分布规律相似,都呈现出向低海拔、平缓坡和各坡向发展的趋势,其中2004年>25°坡度区域竹林碳储量和面积变化最明显,2004年>25°坡度区域竹林碳储量增加量占总增加量的79.53%,竹林面积增加量占总增加量的78.18%,表明退耕还林工程的实施是区域竹林碳储量与面积增加的驱动力;竹林碳密度呈现先降后升总体略微降低的特点,1994年碳密度最高为33.76tC·hm-2,2004年下降为33.25tC·hm-2,2007年又恢复到33.67tC·hm-2;利用克里金插值(Kriging)对模型进行优化可在一定程度上提高预测精度。  相似文献   

Rapid detection of self-biting disease of mink by specific sequence-characterized amplified regions     

LIU Zong-yue  NING Fang-yong  YANG Hong-yan  WEI Lai  BAI Xiu-juan 《林业研究》2011,21(1):123-126

Self-biting disease occurred in most farmed fur animals in the world. The mechanism and rapid detection method of this disease has not been reported. We applied bulked sergeant analysis (BSA) in combination with RAPD method to analyze a molecular genetic marker linked with self-biting trait in mink group. The molecular marker was converted into sequence-characterized amplified regions (SCAR) marker for rapid detection of this disease. A single RAPD marker A8 amplified a specific band of 263bp in self-biting minks, which was designated as SRA8-250, and non-specific band of 315bp in both self-biting and healthy minks. The sequences of the bands exhibited 75% and 88% similarity to Canis familiarizes major histocompatibility complex (MHC) class II region and Macaca mulatta MHC class I region, respectively. A SCAR marker SCAR-A8 was designed for the specific fragment SRA8-250 and validated in 30 self-biting minks and 30 healthy minks. Positive amplification of SCAR-A8 was detected in 24 self-biting minks and 12 healthy minks. χ2 test showed significant difference (p<0.01) in the detection rate between the two groups. This indicated that SRA8-250 can be used as a positive marker to detect self-biting disease in minks. Furthermore, the finding that self-biting disease links with MHC genes has significant implications for the mechanism of the disease.  相似文献   

荸荠秆枯病菌生物学性状研究     

梁钧  赖传雅  黄健平  白志良  李界秋  杨少波 《广西农业生物科学》1995,(4)

荸荠秆枯菌（ＣｙｌｉｎｄｒｏｓｐｏｒｉｕｍｅｌｅｏｃｈａｒｉｄｉｓＬｅｎｔｚ）生长发育温度８～３５℃，最适于菌丝体生长、产孢的温度为２５～３０℃，２０～２８℃孢子萌芽率较高。致死温度４６℃１０ｍｉｎ。病菌在ＲＨ７５％以上生长良好，其产孢量则随湿度升高而增加。ＲＨ８１％以上孢子可以发芽，饱和湿度再加水滴时发芽率最高。ＰＨ４．５０～９．１３均较适于病菌生长，产孢量则以在ＰＨ６．９４～９．１３时较多，ＰＨ４．２０～７．６０较适宜孢子发芽。连续黑光灯照射有促进孢子形成和菌丝体旺盛生长的作用。此菌对多种单糖、双糖和多糖都能利用，以蜜二糖、麦芽糖、葡萄糖等产孢量较多。适于病菌生长和产孢的氮源是甘氨酸、ＤＬ－α－氨基丙酸和氨基乙酸，脲素较差。  相似文献   

温度势梯度下土壤水平一维水盐运动特征的实验研究   总被引：10，自引：0，他引：10  

李毅  王文焰  王全九  邵明安  张建丰  来剑斌 《农业工程学报》2002,18(6):4-8

为了分析温度势梯度作用下的土壤水平一维水盐运动特征,该文对土柱中存在温度势梯度时不同初始含水率下水盐运动的实验结果进行了分析。通过对瞬态温度变化过程和稳态温度形成特征的探讨,研究了温度势梯度存在时水平一维土柱中水盐运动的特点。在温度势梯度影响下土壤水分总体上从暖端向冷端迁移,而土壤盐分则以某位置为临界点在暖端累积,在该临界位置前后土壤含盐量与初始含盐量相比,由高于该值到低于该值,并在接近冷端处与初始值相同  相似文献