The isolation of salmonellas from piggery waste water after orthodox pondage treatment   总被引：2，自引：0，他引：2  

DP HENRY  AJ FROST  D. O'BOYLE  RDA CAMERON 《Australian veterinary journal》1995,72(12):478-479

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Streptococcus dysgalactiae infection in calves     

DP RYAN  JT ROTHWELL  MAZ HORNITZKY 《Australian veterinary journal》1991,68(6):210-210

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Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)     

Rambozzi L  Menzano A  Lavin S  Rossi L 《Veterinary research》2004,35(6):701-708

Scabies is a major threat to the well being of mountain-dwelling Bovid hosts, Rupicapra rupicapra and Rupicapra pyrenaica. Severe outbreaks are in progress over a significant part of their distribution area and resource managers demand improved methods to monitor, analyse and possibly forecast the spread and effects of scabies at the population level. An amplified capture enzyme-linked immunosorbent assay was developed to detect antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.) serum. The method used the biotin-avidin amplification system and was validated on a panel of 144 serum samples, of which 40 were obtained from scabietic and 104 from healthy unexposed individuals originating from a scabies-free area. The antigen, a whole body extract of the various developmental stages of S. scabiei, was prepared from mites actively leaving the skin lesions of naturally infested red foxes (Vulpes vulpes). The resulting LAB-ELISA was characterised by 93% sensitivity, 97% specificity and a high degree of repeatibility. A single seroreactor was found amongst 32 chamois affected with skin pathologies other than scabies, including infestations by other Acarina (Trombicula spp. and Ixodid ticks). Antibodies to S. scabiei were present in 26 out of 169 sera (15.4%) obtained by clinically healthy chamois within a scabies outbreak area, indicating that asymptomatic infestations by S. scabiei can be revealed by serological methods in the studied Caprinae hosts.  相似文献   

Evaluation of Doppler ultrasonography for the measurement of blood flow in young loggerhead sea turtles (Caretta caretta)     

Valente AL  Parga ML  Espada Y  Lavin S  Alegre F  Marco I  Cuenca R 《Veterinary journal (London, England : 1997)》2008,176(3):385-392

The aim of this study was to identify ultrasound accessible blood vessels in the loggerhead sea turtle (Caretta caretta) and describe their Doppler waveform patterns, peak systolic velocity, mean velocity, systolic/diastolic ratio as well as pulsatility and resistive indices. B-mode, colour and pulsed-wave Doppler examinations were performed on 10 turtles. Flow measurements were recorded for the left and right aorta, epigastric and internal iliac arteries, and right hepatic vein. Additionally, the large blood vessels of three dead turtles were injected with latex and dissected for anatomical support. A parabolic flow velocity profile was observed in all arteries. The waveforms of the right and left aortic arteries showed an unusual pattern when compared with mammals. The hepatic vein flow velocity waveform of the loggerhead sea turtle was found to be similar to that in the dog, although the flow velocity in the C-wave was higher than that in the A-wave. The low resistance flow pattern observed suggests that the loggerhead sea turtle's organs require a continuous blood supply.  相似文献   

Blood group system in a captive population of European wildcats (Felis silvestris)     

Silvestre-Ferreira AC  Marco I  Daussa B  Piñol C  Lavin S  Pastor J 《The Veterinary record》2006,159(17):567-568

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Solid phase microextraction application in gas chromatography/olfactometry dilution analysis   总被引：3，自引：0，他引：3  

Deibler KD  Acree TE  Lavin EH 《Journal of agricultural and food chemistry》1999,47(4):1616-1618

Gas chromatography/olfactometry (GC/O) based on dilution analysis (e. g., CharmAnalysis or aroma extraction dilution analysis) gives an indication of what compounds are most important (most potent) to the aroma of foods. The application of solid phase microextraction to the preparation of samples for GC/O dilution analysis was shown to be feasible by varying the fiber thickness and length to achieve various absorbant volumes.  相似文献   

The nucleotide sequence of the genes, fanE and fanF of Escherichia coli K99 fimbriae.     

E Ono  M F Lavin  M Naiki 《The Japanese journal of veterinary research》1991,39(1):1-10

K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing several DNA fragments produced by endonuclease or exonuclease digestion. The fanE gene encoded 227 amino acids containing 20 amino acids of signal peptide starting from GTG (valine) and showed a homology to fanA-fanB. The fanF gene encoded 271 amino acids containing 20 amino acids of signal peptide starting from ATG (methionine) and showed homologies to the fanD gene, fimbrillin gene of F41, adhesin gene of P fimbriae (papG) and adhesin gene of Type 1 fimbriae (fimH). E and F subunits had fifteen and fourteen hydrophobic domains, respectively, which periodically appeared possibly forming a hydrophobic region.  相似文献   

Comparison of DNA-spot hybridization, cell culture and direct immunofluorescence staining for the diagnosis of avian chlamydiae   总被引：1，自引：0，他引：1  

P Timms  F W Eaves  B J Rodwell  M F Lavin 《Veterinary microbiology》1988,18(1):15-25

DNA-spot hybridization, cell culture and direct immunofluorescence staining were compared for the detection of avian Chlamydia psittaci strains in cell culture dilutions and in routine samples submitted for diagnosis. With dilutions of infected cell culture material, growth in BGM cells was by far the most sensitive technique, detecting 0.01 infected cells (20 elementary bodies) ml-1. DNA-spot hybridization and direct immunofluorescence staining were of approximately equal sensitivity, both detecting 16 infected cells (3.2 x 10(4) elementary bodies) per ml-1. When 27 avian liver and spleen samples were assayed, all 3 tests performed similarly (13 positive and 12 negative by all 3 tests). This suggests that in most avian samples presented for diagnosis, sufficient numbers of chlamydiae are present to allow any of the test to the be used. Thus, the direct immunofluorescence staining method is currently the test of choice for routine diagnosis since it is available in kit form, is relatively simple and quick to perform, and like DNA-spot hybridization, detects non-viable as well as viable organisms. However, if low levels of chlamydiae are to be effectively detected, such as in carrier birds or birds with recently acquired infections, then cell culture should be used.  相似文献   

Wild fallow deer (Dama dama) as definitive hosts of Fasciola hepatica (liver fluke) in alpine New South Wales     

DJ Jenkins  A Baker  M Porter  S Shamsi  DP Barton 《Australian veterinary journal》2020,98(11):546-549

To determine the extent to which wild deer are contributing in the transmission of Fasciola hepatica (liver fluke) livers from deer shot by hunters, farmers undertaking population control on their farms and vertebrate pest controllers were collected and frozen. The livers were later thawed, sliced and examined for the presence of adult flukes or evidence of past infection. Livers from 19 deer were examined (18 fallow [Dama dama] and one sambar [Rusa unicolor]). Seventeen of the fallow deer were animals collected on farms near Jindabyne, New South Wales. The remaining fallow deer was collected in the Australian Capital Territory and one sambar deer was collected in north-eastern Victoria. Nine of the 17 deer (53%) from the Jindabyne area were either infected with Fasciola hepatica (liver fluke) or had thickened bile ducts indicating past infection. Infection levels in the infected animals varied widely from 3 liver fluke to over 50 per liver. No sign of infection was present in the deer from the Australian Capital Territory or Victoria. Fallow deer are wide-spread in the Jindabyne area and their population is increasing. It is likely their contribution to the maintenance and distribution of F. hepatica to livestock in the Jindabyne area, and in other livestock rearing areas of south-eastern Australia, is important and increasing.  相似文献   

ANTIRABIC IMMUNIZATION WITH CULTURE VIRUS RENDERED AVIRULENT BY ULTRA-VIOLET LIGHT     

Hodes HL  Lavin GI  Webster LT 《Science (New York, N.Y.)》1937,86(2237):447-448

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