Development of nursery culture techniques for the mud crab Scylla paramamosain (Estampador)     

Vu Ngoc Ut  Lewis Le Vay  Truong Trong Nghia  & Tran Thi Hong Hanh 《Aquaculture Research》2007,38(14):1563-1568

Cannibalism is one of the main causes of mortality in the culture of the mud crab Scylla paramamosain, particularly in the early post‐larval and juvenile stages when the densities of hatchery‐reared crabs may be very high before stocking into ponds or release into the wild for stock enhancement. In a series of experiments investigating cannibalism mitigation, the influence of stocking density, the effectiveness of sand substrate, brick and shell shelters and feed type were compared in culture of crabs from instar 1 for short nursery periods of 15–30 days. Inclusion of brick and shell shelters significantly increased survival over sand substrate alone. However, inclusion of shelters did not affect growth rates. In scaled‐up nursery production in lined‐ponds, with shelters, live Artemia biomass and fresh chopped shrimp or tilapia were found to be equally effective feeds for juvenile crabs stocked at a density of 70 m⁻² from instar 1 and grown for 30 days [52–66% survival, 21.6–24.6 mm carapace width (CW)]. In an extended nursery period for a further 30 days, crabs of 22 mm CW, stocked at 30 m⁻² in the same ponds, attained a final size of 34.5–36.2 mm CW with a survival of 64.3–67.0% using the same feeds.  相似文献   

Reference intervals and age‐related changes for venous biochemical,hematological, electrolytic,and blood gas variables using a point of care analyzer in 68 puppies     

Mauria A. O'Brien DVM  DACVECC  Maureen A. McMichael DVM  DACVECC  Kevin Le Boedec DVM  MS  George Lees DVM  MS  DACVIM 《Journal of Veterinary Emergency and Critical Care》2014,24(3):291-301

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投喂频率对凡纳滨对虾生长和水质的影响     

叶乐  林黑着  李卓佳  吴开畅  文国樑  马之明  朱昌福 《南方水产》2005,1(4):55-59

研究了不同投喂频率(1,2,3,4和5次·d-1)对凡纳滨对虾生长、存活率和水质的影响。试验在室内2m3的圆形水泥池(d=1.6m)中进行,采用全封闭养殖模式,凡纳滨对虾(初始重量为0.24g)养殖密度为50尾·m-3,投喂人工配合饲料(粗蛋白含量为42.98%)。通过7周的试验,结果表明,投喂频率为1～4次·d-1时,随着投喂频率的增加,对虾增重率显著增加;投喂频率由4次·d-1增加到5次·d-1时,对虾增重率略有增加,但差异不显著。凡纳滨对虾的成活率在1次·d-1时最低,2次·d-1时最高,2～5次·d-1时随着投喂频率的增加而降低(投喂频率为1和2次·d-1之间、3和4次·d-1之间差异显著)。投喂频率为3次·d-1时饲料系数最低,蛋白质效率最高。随着投喂频率1～3次·d-1增加,饲料系数逐渐显著降低,蛋白质效率显著递增;而投喂频率从3～5次·d-1增加时,饲料系数增大,蛋白质效率逐渐下降(3和4次·d-1差异显著;4和5次·d-1之间差异不显著)。投喂频率对水环境中的pH值、H2S含量、COD、盐度等水质指标没有显著影响;在养殖后期水环境中投喂频率3～5次·d-1的NH 4和NO-2浓度较投喂频率1～2次·d-1时高。  相似文献   

The conservation status of Sabellaria alveolata (L.) (Polychaeta: Sabellariidae) reefs in the Bay of Mont‐Saint‐Michel     

N. Desroy  S. F. Dubois  J. Fournier  L. Ricquiers  P. Le Mao  L. Guerin  D. Gerla  M. Rougerie  A. Legendre 《水产资源保护:海洋与淡水生态系统》2011,21(5):462-471

近海渔业安全救助通信网的核心技术--传输速率对网络阻塞的影响     

李蔚海  张来保  魏乐  苑传林 《水产科学》2004,23(9):44-45

"近海渔业安全救助通信网"(以下简称为"安全通信网")建设开通以来,在渔业安全生产中已经发挥了重要的作用。但随着我国海洋渔业经济结构的变革,现代通信技术的飞速发展,刚刚组建的"安全通信网"日趋落后,在实际运行中引出了诸多问题,难以适应渔业生产和管理的需要。本文仅从技术角度针对"安全通信网"中出现的网络阻塞问题,阐述几点意见。  相似文献   

Purification of chinook salmon (Oncorhynchus tshawytscha) GH for receptor study     

Pierre-Yves Le Bail  Geneviève Boulard  Bruno Barenton  Michel Zygmunt 《Fish physiology and biochemistry》1989,7(1-6):243-251

A method for the purification of chinook Salmon (Oncorhynchus tshawytscha) GH, which retains its biological activity, is described. The biological activity was investigated with an established radioreceptor assay using liver membranes from pregnant rabbits and bovine GH as standard and labelled hormone. The enrichment of the preparation was checked with electrophoresis (SDS-PAGE). Extraction and further steps were carried out using low molarity alkaline buffer (pH 8–10, M = 100 mM). Three chromatography steps were performed (Concanavalin-A sepharose, Bio-gel P60, DEAE). Ion exchange chromatography was performed under isocratic conditions (using a 50 cm column). Two isoforms (sGH1 and sGH2) were isolated. The purification yield is 0.7% compared to lyophilized pituitaries. The molecule is homogeneous in SDS-PAGE. Contamination by prolactin, gonadotrophin and corticotrophin is negligible (< 0.5%). It could be demonstrated that the biological activity of the preparation is maintained since this preparation stimulates the growth of juvenile trout (Salmo gairdneri) and binds specifically (35%) to trout liver membranes.  相似文献   

Trypsin enzyme activity during larval development of Litopenaeus vannamei (Boone) fed on live feeds   总被引：1，自引：0，他引：1  

A C Puello-Cruz  R S Sangha  D A Jones  & L Le Vay 《Aquaculture Research》2002,33(5):333-338

Larval stages of the Pacific white shrimp, Litopenaeus vannamei (Boone) were fed standard live diets of mixed microalgae from the first to the third protozoea (PZ1 to PZ3), followed by Artemia nauplii until post‐larvae 1 (PL1). Trypsin enzyme activity for each larval stage was determined using N‐α‐p‐toluenesulphonyl‐l ‐arginine methyl ester (TAME) as a substrate. Results were expressed as enzyme content to assess ontogenetic changes during larval development. Tissue trypsin content (IU µg^?1 DW for each larval stage) was significantly highest at the PZ1 stage and declined through subsequent stages to PL1. This contrasts with previously observed patterns of trypsin development in Litopenaeus setiferus (Linnaeus) and other penaeid genera, which exhibit a peak in trypsin activity at the third protozoea/first mysis (PZ3/M1) larval stage. Litopenaeus vannamei larvae transferred to a diet of Artemia at the beginning of the second protozoea (PZ2) stage were significantly heavier on reaching the first mysis stage (M1) than those fed algae, while survival was not significantly different between treatments. At both PZ2 and PZ3 stages, trypsin content in larvae feeding on Artemia was significantly lower than in those feeding on algae. The rapid decline in trypsin content from PZ1 and the flexible enzyme response from PZ2 suggest that L. vannamei is physiologically adapted to transfer to a more carnivorous diet during the mid‐protozoeal stages.  相似文献   

海南省琼中县橡胶园土壤磷素空间变异特征及磷肥分区管理分析     

覃怀德  吴炳孙  吴敏  韦家少  何鹏  王大鹏  高乐 《热带作物学报》2014,35(2):369-374

运用地统计学和GIS相结合对琼中县橡胶园土壤磷素营养进行了空间变异特征及分区管理研究。结果表明,琼中县胶园各土层土壤全磷和有效磷含量的最大相关距离是17.23~52.09 km,全磷含量以大块状变异为主,有效磷含量各等级的分布为大、小块状交叉分布;琼中县橡胶园0~20 cm土层、20~40 cm土层全磷含量处于第五等级的极低水平的比例分别为91.22%和82.8%,0~20 cm土层有效磷含量多数处于中等偏上水平,而20~40 cm土层有效磷含量处于第5等级及以下的极低水平的比例为60.6%;应用空间叠加方法,进行了橡胶园土壤磷素管理分区,琼中县胶园可分为11个管理分区,在胶园施肥管理中应根据不同管理分区的特点采取不同的磷素营养管理措施。  相似文献   

成都平原超级稻超高产群体产量形成与物质生产特点研究     

兰汉军等 《中国稻米》2014,(1):41-43

对Ⅱ优602、德香4103、Q优8号等3个超级稻品种不同产量级别群体的产量形成及干物质生产特点进行了研究。结果表明,超级稻品种从中产(产量≤9.0 t/hm2)实现高产(产量9.0~10.5 t/hm2)关键在于每穗粒数提高后库容的增大,并同时提高结实率;而要实现超高产(产量≥10.5 t/hm2)则有赖于大库条件下结实率的进一步提高。此外,超级稻品种产量分别与分蘖临界期后各阶段的干物质积累量存在显著或极显著的正相关关系,超级稻品种高产群体获得高产的关键是提高抽穗后物质生产能力,而要进一步实现超高产则需要抽穗前后干物质生产能力的协同提高。  相似文献   

Pseudaboydins A and B: Novel Isobenzofuranone Derivatives from Marine Fungus Pseudallescheria boydii Associated with Starfish Acanthaster planci     

Wen-Jian Lan  Wei Liu  Wan-Ling Liang  Zeng Xu  Xiu Le  Jun Xu  Chi-Keung Lam  De-Po Yang  Hou-Jin Li  Lai-You Wang 《Marine drugs》2014,12(7):4188-4199

Two novel isobenzofuranone derivatives, pseudaboydins A (1) and B (2), along with five known compounds, including (R)-2-(2-hydroxypropan-2-yl)-2,3-dihydro-5-hydroxybenzofuran (3), (R)-2-(2-hydroxypropan-2-yl)-2,3-dihydro-5-methoxybenzofuran (4), 3,3′-dihydroxy-5,5′-dimethyldiphenyl ether (5), 3-(3-methoxy-5-methylphenoxy)-5-methylphenol (6) and (−)-regiolone (7), were isolated from the culture broth of the marine fungus, Pseudallescheria boydii, associated with the starfish, Acanthaster planci. Their structures were elucidated primarily based on NMR and MS data. The absolute configurations of 1–4 were determined by CD spectroscopy and single-crystal X-ray diffraction studies. The cytotoxic and antibacterial activities of 1–4 were evaluated. Pseudaboydin A (1) showed moderate cytotoxic activity against human nasopharyngeal carcinoma cell line HONE1, human nasopharyngeal carcinoma cell line SUNE1 and human glandular lung cancer cell line GLC82 with IC₅₀ values of 37.1, 46.5 and 87.2 μM, respectively.  相似文献