Nuclear magnetic resonance spectroscopic study of beta-lactoglobulin interactions with two flavor compounds,gamma-decalactone and beta-ionone     

Lübke M  Guichard E  Tromelin A  Le Quéré JL 《Journal of agricultural and food chemistry》2002,50(24):7094-7099

Interactions between a well-characterized protein, beta-lactoglobulin, and two flavor compounds, beta-ionone and gamma-decalactone, were studied by 2D NMR spectroscopy. NMR spectra were recorded in aqueous solution (pH 2.0, 12 mM NaCl, 10% D(2)O) under conditions such that beta-lactoglobulin is present in a monomeric state. TOCSY and NOESY spectra were recorded on the protein and the complexes between protein and ligands. The spectra of the NH-CH(alpha) region showed the cross-signals due to the coupling between N- and C-bonded protons in the polypeptide backbone. The observed chemical shift variations in the presence of ligands can be assigned to changes in the protein conformation. It appears that the side chains of several amino acids are affected by binding of gamma-decalactone point into the central cavity (Leu46, Ile56, Met107, and Gln120), whereas binding of beta-ionone affects amino acids located in a groove near the outer surface of the protein (Leu104, Tyr120, and Asp129), as illustrated by molecular visualization. This NMR study provides precise information of the location of binding and confirms the existence of two different binding sites for aroma compounds on beta-lactoglobulin, which was suggested in previous competition studies by fluorometry or affinity chromatography and by structural information obtained from infrared spectroscopy.  相似文献   

Improved method for determination of polynuclear aromatic hydrocarbons in pharmacopoeial paraffin and mineral oils.     

A Geahchan  I Le Gren  P Chambon  R Chambon 《Journal of the Association of Official Analytical Chemists》1991,74(6):968-973

An improved method has been developed for quantitative determination of polynuclear aromatic hydrocarbons (PAHs) in pharmacopoeial paraffin and medicinal white oil samples. This new method combines 2 liquid-liquid partition and adsorption chromatography procedures with a 2-step purification on Sephadex LH 20 and liquid chromatography with fluorometric determination. Selective elution of PAHs results in absence of background fluorescence. The minimum detectable level ranges from 0.2 ppt for benzofluoranthene isomers to 200 ppt for acenaphthene. Recoveries of PAHs added at 7 ppm varied from 92.1 to 111.4%. When a variety of medicinal white oil samples were analyzed by this improved method, 27 PAHs were identified, including 11 suspected carcinogens. Their identities were confirmed by capillary gas chromatography.  相似文献   

Inhibition of apple polyphenol oxidase activity by procyanidins and polyphenol oxidation products   总被引：4，自引：0，他引：4  

Le Bourvellec C  Le Quéré JM  Sanoner P  Drilleau JF  Guyot S 《Journal of agricultural and food chemistry》2004,52(1):122-130

The rate of consumption of dissolved oxygen by apple polyphenol oxidase in cider apple juices did not correlate with polyphenol oxidase activity in the fruits and decreased faster than could be explained by the decrease of its polyphenolic substrates. The kinetics parameters of a crude polyphenol oxidase extract, prepared from apple (Braeburn cultivar), were determined using caffeoylquinic acid as a substrate. Three apple procyanidin fractions of n 80, 10.5, and 4 were purified from the parenchyma of cider apples of various cultivars. Procyanidins, caffeoylquinic acid, (-)-epicatechin, and a mixture of caffeoylquinic acid and (-)-epicatechin were oxidized by reaction with caffeoylquinic acid o-quinone in order to form oxidation products. All the fractions were evaluated for their inhibitory effect on PPO activity. Native procyanidins inhibited polyphenol oxidase activity, the inhibition intensity increasing with n. The polyphenol oxidase activity decreased by 50% for 0.026 g/L of the fraction of n 80, 0.17 g/L of the fraction of n 10.5, and 1 g/L of the fraction of n 4. The inhibitory effect of oxidized procyanidins was twice that of native procyanidins. Oxidation products of caffeoylquinic acid and (-)-epicatechin also inhibited polyphenol oxidase.  相似文献   

Metabolite profiling of tomato (Lycopersicon esculentum) using 1H NMR spectroscopy as a tool to detect potential unintended effects following a genetic modification   总被引：5，自引：0，他引：5  

Le Gall G  Colquhoun IJ  Davis AL  Collins GJ  Verhoeyen ME 《Journal of agricultural and food chemistry》2003,51(9):2447-2456

  相似文献   

The SAHGA model to calculate the Spatial Ammoniacal Heterogeneity at the soil surface after fertiliser Granule Application     

Edith?Le?CadreEmail author  Sophie?Génermont  Farooq?Azam  Sylvie?Recous 《Biology and Fertility of Soils》2004,40(3):178-180

After dissolution of fertiliser granules, a high nitrogen concentration is recovered in the immediate vicinity of granules, which may enhance damaging processes like nitrite accumulation or ammonia volatilisation. Based on the diffusion equations of Cranck, the granule-soil microsite was modelled to obtain the actual fertilised surface plot and the effective rate of N application on this surface. Parameterisation of the diffusion coefficient of solutes consisted of a temperature and soil texture correction. The model was tested against an experimental data set obtained from soil incubations at two soil water contents (21.2% m³ m^–3 and 28.3% m³ m^–3) and two temperatures (4°C and 25°C) by comparing NH₄⁺ recovery at various distances from the granules. The simulated radius of the granule-soil microsite was more affected by the water content than by the temperature. The model is very accurate because 95–100% of total NH₄⁺ applied was recovered in the modelled surface depending on the experimental conditions (temperature and water content). The model was simple enough to be easily integrated into larger models dealing with surface-applied granule fertilisers.  相似文献   

The occurrence of friabilins in triticale and their relationship with grain hardness and baking quality   总被引：3，自引：0，他引：3  

Ramírez A  Pérez GT  Ribotta PD  León AE 《Journal of agricultural and food chemistry》2003,51(24):7176-7181

Grain hardness is a quality parameter in wheat and other cereals. In wheat, a group of M(r) 15 000 proteins called friabilins have been shown to be related to grain hardness. The objective of this study was to determine the presence of friabilins on starch granules of different triticale lines and their relationship with grain texture and baking quality. The triticale lines studied have a wide range of hardness, which presented correlation with baking quality parameters such as damaged starch and solvent retention capacity. All of the triticale lines contained friabilins on the starch granules. However, the correlation between hardness and friabilin content was not observed, suggesting that these proteins would not be directly involved in grain texture determination of triticale. Consequently, friabilin content did not have any relation with cookie quality in triticale flours, but it could be related to breadmaking quality because it has a positive correlation with the sodium dodecyl sulfate sedimentation index.  相似文献   

EXCHANGEABLE PHOSPHORUS, ESTIMATES OF IT FROM AMORPHOUS IRON OXIDES, AND SOIL SOLUTION PHOSPHORUS, IN RELATION TO PHOSPHORUS TAKEN UP BY MAIZE     

P. H. Le  MARE 《European Journal of Soil Science》1981,32(2):285-299

Amounts of phosphorus taken up by maize plants during 23 days from an Entisol, two Alfisols and an Ultisol from Nigeria, each with three histories of management, were related to the concentration of soil phosphorus extracted in 0.01 m CaCl₂; but the relationships differed between kinds of management. Phosphorus in plants 51 days old was not related to concentration. Relationships of plant phosphorus with isotopically exchangeable phosphorus, and with phosphorus extracted from the soils by anion exchange resin, were poor in young plants but were good in the older plants, irrespective of field management. Amounts of phosphorus in the older plants were also well related to estimates of exchangeable phosphorus computed from P in solution and amounts of amorphous iron oxides extracted by ammonium oxalate from the soils, using equations derived in an earlier investigation with a larger group of soils. Thus estimates of exchangeable phosphorus made from simple measurements of the soil constituents that largely control it, and from extraction by resins, are potentially useful to indicate the ability of soils to supply phosphate to crops, and hence to guide fertilizer policies.  相似文献   

Tests on Cracking Behaviors of Reinforced Concrete Beams Underlong- Term Sulphate Attack     

GUO Le - gong  GUO Le - ning  HUANG Zhen - guo 《保鲜与加工》2006,(3):71-73

In this paper,six reinforced concrete beams dismounted from their worksites under long-term sulphate attack are studied.The authors analyzed the working properties and cracking moment of the reinforced concrete beams under long-term sulphate attack,suggesting that the reinforced concrete beams under long-term sulphate attack may have more favorable cracking-resistant behavior in some circumstances.  相似文献   

RAPD variability in rice (Oryza sativa L.) plants derived from desiccation-tolerant calli     

Dinh Thi Phong  Le Thi Muoi  Le Tran Binh 《Euphytica》2001,121(3):297-303

Genetic modification from selfed progenies of 18 rice (Oryza sativa L.) plants regenerated from callus tissues which survived desiccation, were investigated at the DNA level using the random amplified polymorphic DNA (RAPD) method. Twelve 10-mer random primers were used to amplify DNA of progenies from the regenerated plants, and a total of 228 PCR products and 1780 DNA fragments were obtained by primers, generating between four to thirteen major bands. The size of the amplified fragments ranged from 0.2 to 2.55 kb. The results showed that 10 out of 12 primers produced polymorphic bands, two primers (RA31 and RA185) showed no polymorphism among plants tested. A dendrogram of the genetic distance was constructed based on their polymorphism, demonstrating that somaclonal variation exists in rice plants regenerated from callus which survived the desiccation treatment. Part of this variation can be useful in rice breeding. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Fungitoxic effect of scopolin and related coumarins on Sclerotinia sclerotiorum. A way to overcome sunflower head rot     

E. Prats  M. E. Bazzalo  A. León  J. V. Jorrín 《Euphytica》2006,147(3):451-460

Summary The content of coumarins, as probable phytoalexins, was analysed in four sunflower genotypes that ranged in responses to head rot from highly susceptible to highly resistant. Low levels of all coumarins (scopolin, scopoletin and ayapin) were detected in the three most susceptible genotypes irrespective of time after inoculation. However, in the resistant genotype there was a clear time-dependent disease-induced increase of all coumarins that reached a maximum after 10–14 days. Detailed comparison of the most susceptible and the resistant genotype showed that in the resistant but not the susceptible, scopoletin peroxidase activity increased during the course of the experiment. Results confirmed a clear negative correlation between coumarin content and disease symptoms and in particular for scopolin. Furthermore we show for the first time that scopolin is inhibitory to Sclerotinia at similar doses to scopoletin. As scopolin is known to be less phytotoxic than ayapin and scopoletin, its accumulation may well confer head rot resistance with minimal plant damage and might be one of the bases for resistance to Sclerotinia.  相似文献