Effects of rice bran protein concentrate on the growth performance and digestive enzyme activities of jundiá (Rhamdia quelen) (Quoy and Gaimard, 1824)     

Bruno Bianch Loureiro  Fernanda Rodrigues Goulart  Fernanda Teixeira Macagnan  Sharine Nunes Descovi  Naglezi de Menezes Lovatto  Thais Soares dos Santos  Marina Osmari Dalcin  Leila Picolli da Silva 《Aquaculture Nutrition》2019,25(5):1115-1123

The aim of this study was to assess the effect of increasing inclusion levels (100, 150, 200 and 300 g/kg) of rice bran protein concentrate (RBPC) in jundiá (Rhamdia quelen) diets using growth, body composition, somatic indices and digestive enzyme activity as parameters. Five isoproteic (370.08 ± 0.04 g/kg) and isocaloric (13.38 ± 0.04 MJ/kg) diets were formulated with four replicates per treatment. After acclimation, 500 jundiá juveniles (initial mean weight of 6.28 ± 0.12 g) were distributed into 20 round polyethylene tanks (280 L) (25 fish per tank) coupled to a thermoregulated water recirculating system. The fish were fed experimental diets three times daily (at 9:00, 13:00 and 17:00 hr) to apparent satiation. At the end of the trial (45 days), no significant differences were found in the body chemical composition, somatic indices, and trypsin and chymotrypsin enzymes of the fish fed experimental diets. A lower final weight and a lower condition factor were found in fish fed diets RBPC10 (100 g/kg of RBPC) and RBPC15 (150 g/kg of RBPC). Based on the results of this study, it is clear that the use of RBPC (at high dietary inclusion levels of 200 and 300 g kg^?1) is an effective alternative protein source to fishmeal.  相似文献   

Evaluation of Outer Membrane Vesicles Obtained from Predominant Local Isolate of Bordetella pertussis as a Vaccine Candidate     

Maryam Sadat Soltani  Mojtaba Noofeli  Seyed Reza Banihashemi  Fereshteh Shahcheraghi  Fereshteh Eftekhar 《Iranian Biomedical Journal》2021,25(6):399

Background:Pertussis is a current contagious bacterial disease caused by Bp. Given the prevalence of pertussis, development of new vaccines is important. This study was attempted to evaluate the expression of main virulence factors (PTX, PRN, and FHA) from Bp predominant strains and also compare the expression of these factors in the OMVs obtained from predominant circulating Bp isolate. Methods:The physicochemical features of the prepared OMVs were analyzed by electron microscopy and SDS-PAGE. The presence of the mentioned virulence factors was confirmed by Western blotting. BALB/c mice (n = 21) immunized with characterized OMVs were challenged intranasally with sublethal doses of Bp, to examine their protective capacity. Results:Electron microscopic examination of the OMVs indicated vesicles within the range of 40 to 200 nm. SDS-PAGE and Western blotting demonstrated the expression of all three main protective immunogens (PTX, PRN, and FHA), prevalent in the predominant, challenge, and vaccine strains, and OMVs of the predominant IR37 strain and BP134 vaccine strain. Significant differences were observed in lung bacterial counts between the immunized mice with OMV (30 CFU/lung) compared to the negative control group ((6 10⁴ CFU/lung; p < 0.001). In mice immunized with OMVs (3 µg), the number of lungs recovered colonies after five days dropped at least five orders of magnitude compared to the control group. Conclusion:OMVs obtained from circulating isolates with the predominant profile may constitute a highly promising vaccine quality. They also can be proposed as a potential basic material for the development of new pertussis vaccine candidate. Key Words: Bordetella pertussis, Vaccines, Virulence factors  相似文献   

Encapsulation of Rosmarinus officinalis essential oil and of its main components in cyclodextrin: application to the control of the date moth Ectomyelois ceratoniae (Pyralidae)     

Maha Ben Abada  Abir Soltani  Maroua Tahri  Soumaya Haoual Hamdi  Emna Boushih  Sophie Fourmentin  Hélène Greige-Gerges  Jouda Mediouni Ben Jemâa 《Pest management science》2023,79(7):2433-2442

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