Consequences of a large-scale fragmentation experiment for Neotropical bats: disentangling the relative importance of local and landscape-scale effects     

Ricardo Rocha  Milou Groenenberg  Paulo E. D. Bobrowiec  Mar Cabeza  Jorge M. Palmeirim  Christoph F. J. Meyer 《Landscape Ecology》2017,32(1):31-45

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Chitosan-Based Films with 2-Aminothiophene Derivative: Formulation,Characterization and Potential Antifungal Activity     

Vernica da Silva Oliveira  Meringela Miranda da Cruz  Gabriela Suassuna Bezerra  Natan Emanuell Sobral e Silva  Fernando Henrique Andrade Nogueira  Guilherme Maranho Chaves  Jos Lamartine Soares Sobrinho  Francisco Jaime Bezerra Mendona-Junior  Bolívar Ponciano Goulart de Lima Damasceno  Attilio Converti  dley Antonini Neves de Lima 《Marine drugs》2022,20(2)

In this study, films of chitosan and 2-amino-4,5,6,7-tetrahydrobenzo[b]thiophene-3-carbonitrile (6CN), a 2-aminothiophene derivative with great pharmacological potential, were prepared as a system for a topical formulation. 6CN-chitosan films were characterized by physicochemical analyses, such as Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and scanning electronic microscopy (SEM). Additionally, the antifungal potential of the films was evaluated in vitro against three species of Candida (C. albicans, C. tropicalis, and C. parapsilosis). The results of the FTIR and thermal analysis showed the incorporation of 6CN in the polymer matrix. In the diffractogram, the 6CN-chitosan films exhibited diffraction halos that were characteristic of amorphous structures, while the micrographs showed that 6CN particles were dispersed in the chitosan matrix, exhibiting pores and cracks on the film surface. In addition, the results of antifungal investigation demonstrated that 6CN-chitosan films were effective against Candida species showing potential for application as a new antifungal drug.  相似文献   

Statistical associations between restriction fragment length polymorphisms and quantitative traits in beef cattle.   总被引：5，自引：0，他引：5  

J L Rocha  J F Baker  J E Womack  J O Sanders  J F Taylor 《Journal of animal science》1992,70(11):3360-3370

Data on 41 traits from 677 animals produced in a five-breed diallel were matched with genotypes for five marker-loci provided by restriction fragment length polymorphisms to detect quantitative effects associated with the markers, following three different designs based on inbred lines, half-sib families, and on assumptions of the markers being quantitative trait loci (QTL). Three growth hormone-TaqI alleles, B, C, and D, in high frequencies in this sample of the Brahman breed, were associated with decreases in birth weight, as a maternal trait (P < .01), and decreases in shoulder width at birth (P < .05). Among F2 Angus-Brahman and Brahman-Hereford cows, homozygotes for the B, C, or D alleles gave birth to calves 4.0 kg lighter than cows homozygous for the A allele, an effect that amounts to the magnitude of the corresponding breed difference in the diallel, and represents one phenotypic SD. A putative cytoplasmic effect seems to interact (P = .02) with this effect on maternal birth weight. Also, at birth, F2 calves homozygous for the B, C, or D alleles were .8 cm narrower at the shoulders than those homozygous for the A allele, after adjusting for birth weight. Significant associations (P < .05) between the parathyroid hormone-MspI marker and measures of body size were detected, as well as an effect on weaning weight (P = .03) as a maternal trait, whose magnitude (17.5 kg) equals the Brahman vs Angus and Hereford breed difference, as quantified in the diallel, and represents .8 of a phenotypic SD. No significant associations were found for three other marker-loci (prolactin-MspI, osteonectin-EcoRI, and keratin VI-MspI). Restriction fragment length polymorphisms have the potential to provide new insights and useful applications to animal breeding, but, as in this work, small sample sizes, extreme susceptibility to Type I errors, and different types of possible confounding obfuscate the conclusions that can be drawn from studies of limited scope and less than ideal planning.  相似文献   

A nontoxic derivative of Bothrops jararaca venom suitable to generate antibodies against the native venom.     

O A Rocha  A F Bicalho  J N Silveira  E S Lopes  I F Heneine 《DTW. Deutsche tier?rztliche Wochenschrift》1992,99(4):143-145

Bothrops jararaca venom was toxoided by stepwise iodination with cold iodine, and doses up to 30 LD50 were non-lethal by i.p. route (BICALHO et al., 1990). Groups of mice injected chronically with the native, or the iodinated venom, have been subjected to histological examination. In the native group, in the spleen, around the white pulp, an acellular, amorphous eosinophilic substance, metachromatic to Methyl Violet, PAS positive, and dichroic to Congo Red under polarized light, was present. Strong congestion in the liver, kidneys and lungs was found. The salivary glands were replenished with an amorphous substance in the serosal acini. The groups injected with the iodinated venom only show discrete alterations, more akin to the control group. The anavenin was immunogenic. Antibody generation in mice and rabbits was detected by ELISA. In mice, active protection against challenge with native venom was presented. The iodinated venom generated a rabbit antiserum with strong lines in gel immunoprecipitation against the lethal venom. A minimum neutralization titer of 2.3 mg ml-1 against the native venom was attained in the first cycle (28 days) of immunization. After 3 cycles (100 days), the protection rose to 5.1 mg ml-1.  相似文献   

Inclusion of licuri meal in the diet of pasture dairy cows     

Costa  Evely Giovanna Leite  da Silva  Fabiano Ferreira  Silva  Robério Rodrigues  Porto  Antonio Ferraz  Santiago  Bismarck Moreira  Rocha  Leone Campos  Cruz  Aline Gonçalves  Guedes  Anna Caroline Ferreira  Neto  Teotônio Martins  Vieira  Edvaldo Alves 《Tropical animal health and production》2019,51(8):2505-2511

Tropical Animal Health and Production - The objective of this study was to evaluate the inclusion of increasing levels of licuri meal as a substitute for soybean bran in the diet of pasture-based...  相似文献   

Effect of l‐glutamic acid supplementation on performance and nitrogen balance of broilers fed low protein diets          下载免费PDF全文

R. M. Bezerra  F. G. P. Costa  P. E. N. Givisiez  E. R. Freitas  C. C. Goulart  R. A. Santos  J. G. Souza  P. A. Brandão  M. R. Lima  M. L. Melo  V. P. Rodrigues  E. T. Nogueira  D. V. G. Vieira 《Journal of animal physiology and animal nutrition》2016,100(3):590-600

The aim of this study was to evaluate the effect of protein reduction and supplementation of l ‐glutamic acid in male broiler diets. A total of 648 chicks of the Cobb 500 strain were distributed in a completely randomized design with six treatments and six replications with eighteen birds per experimental unit. The study comprised pre‐starter (1–7 days), starter (8–21 days), growth (22–35 days) and final (36–45 days) phases. The first treatment consisted of a control diet formulated according to the requirements of essential amino acids for each rearing phase. The second and third treatments had crude protein (CP) reduced by 1.8 and 3.6 percentage points (pp) in relation to the control diet respectively. In the fourth treatment, l ‐glutamic acid was added to provide the same glutamate level as the control diet, and in the last two treatments, the broilers were supplemented with 1 and 2 pp of glutamate above that of the control diet respectively. The reduction in CP decreased the performance of broilers and the supplementation of l ‐glutamic acid did not influence performance when supplied in the diets with excess of glutamate. The lowest excreted nitrogen values were observed in the control diet, and treatments 2 and 3, respectively, in comparison with treatments with the use of l ‐glutamic acid (5 and 6). Retention efficiency of nitrogen was better in the control diet and in the treatment with a reduction of 1.8 pp of CP. It was verified that the serum uric acid level decreased with the CP reduction. A reduction in CP levels of up to 21.3%, 18.8%, 18.32% and 17.57% is recommended in phases from 1 to 7, 8 to 21, 22 to 35 and at 36 to 42 days, respectively, with a level of glutamate at 5.32%, 4.73%, 4.57%, 4.38%, also in these phases.  相似文献   

Use of Liposomal Gentamicin for Treatment of 5 Foals with Experimentally Induced Rhodococcus equi Pneumonia          下载免费PDF全文

N.D. Cohen  S. Giguère  A.J. Burton  J.N. Rocha  L.J. Berghaus  C.N. Brake  A.I. Bordin  M.C. Coleman 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2016,30(1):322-325

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C–N–P interactions control climate driven changes in regional patterns of C storage on the North Slope of Alaska     

Yueyang Jiang  Adrian V. Rocha  Edward B. Rastetter  Gaius R. Shaver  Umakant Mishra  Qianlai Zhuang  Bonnie L. Kwiatkowski 《Landscape Ecology》2016,31(1):195-213

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Genetic parameters for carcass traits and body size in sheep for meat production     

Luiz Antonio Silva Figueiredo Filho  Alan Oliveira Do Ó  José Lindenberg Rocha Sarmento  Natanael Pereira Da Silva Santos  Tatiana Saraiva Torres 《Tropical animal health and production》2016,48(1):215-218

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Intake and digestibility,rumen fermentation,and concentrations of metabolites in steers fed with peanut cake     

Paulo Andrade de Oliveira  Ronaldo Lopes Oliveira  Soraya Maria Palma Luz Jaeger  Meiby Carneiro de Paula Leite  Adriana Regina Bagaldo  Leilson Rocha Bezerra  Braulio Rocha Correia  Nivaldo Barreto de Santana Filho 《Tropical animal health and production》2016,48(2):403-409

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