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41.
Hydrology-oriented silviculture might adapt Mediterranean forests to climatic changes, although its implementation demands a better understanding and quantification on the water fluxes. The influence of thinning intensity (high, medium, low and a control) and its effect on the mid-term (thinned plots in 1998 and 2008) on the water cycle (transpiration, soil water and interception) and growth [basal area increment (BAI)] were investigated in 55-year-old Aleppo pine trees. Thinning enhanced a lower dependence of growth on climate fluctuations. The high-intensity treatment showed significant increases in the mean annual BAI (from 4.1 to 17.3 cm2) that was maintained in the mid-term. Thinning intensity progressively increased the sap flow velocity (v s) in all cases with respect to the control. In the mid-term, an increased functionality of the inner sapwood was also observed. Mean daily tree water use ranged from 5 (control) to 18 (high intensity) l tree?1. However, when expressed on an area basis, daily transpiration ranged from 0.18 (medium) to 0.30 mm (control), meaning that in spite of the higher transpiration rates in the remaining trees, stand transpiration was reduced with thinning. Deep infiltration of water was also enhanced with thinning (about 30 % of rainfall) and did not compete with transpiration, as both presented opposite seasonal patterns. The changes in the stand water relationships after 10 years were well explained by the forest cover metric. The blue to green water ratio changed from 0.15 in the control to 0.72 in the high-intensity treatment, with the remaining treatments in the 0.34–0.48 range.  相似文献   
42.
A shortage of marine raw ingredients, such as fish oil, is predicted in the near future. The use of suitable alternative lipid sources, such as vegetable oils, is crucial for sustainable growth of the aquaculture sector. Three isonitrogenous (50% Crude Protein) and isoenergetic (19 kJ g−1) diets, in which fish oil was gradually replaced by soybean oil (0, 25 and 50% of the oil fraction) were tested for 12 weeks, using European sea bass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss) juveniles. No dietary effects (p>0.05) on growth performance, feed conversion, digestibility or body composition were observed with either species. The results obtained suggest the inclusion of soybean oil up to 50% of the dietary lipid is possible, in diets for sea bass and/or rainbow trout juveniles.  相似文献   
43.
Finfish pens are point sources of dissolved nutrients released from fish metabolism or degradation of solid wastes. Nutrients leaching from uneaten feed and faeces are not usually quantified in mass budgets for these systems, leading to an overestimation of fish retention or deposition to the seabed. In this study, we investigated nutrient leaching from pellets and baitfish feed as well as faeces of southern bluefin tuna (Thunnus maccoyii) into seawater. Faeces were nitrogen depleted (51–54 mg N g−1 dw) and phosphorus enriched (62–72 mg P g−1 dw) compared with feeds (83–111 mg N g−1 dw and 17–21 mg P g−1 dw). Less phosphorus was available for leaching from pellets and faeces of pellet‐fed tuna (5–6%) than from baitfish and faeces of baitfish‐fed tuna (17–21%). The proportion of soluble nitrogen in pellets (15%) was also lower than in baitfish and faeces (35–43%). Leaching loads for a feed conversion ratio of 5 were estimated as 22 and 26 kg N tonne−1 growth when baitfish or pellets were used as feed respectively. Phosphorus loads were estimated as 15 and 4 kg P tonne−1 growth respectively. More than 90% of nitrogen loads, and approximately 50% of phosphorus, are likely to be released into seawater before solid wastes reach the seafloor.  相似文献   
44.
45.
Eight microsatellite loci previously reported were assessed for their utility in parentage assignment in 96 individuals belonging to natural populations of the blackspot seabream Pagellus bogaraveo (Brünnick, 1768) from the Mediterranean and Northeast Atlantic regions. At the mtDNA level, no differentiation was found between these two regions but based on microsatellite data an overall discrete genetic differentiation is perceivable between the two regions separated by the Strait of Gibraltar. The number of alleles per locus ranged from 8 to 30. A database constructed with allele frequency data from six populations was used in a simulation parentage assignment test using the software cervus . The test showed that the number of markers used is enough to perform parentage assignments with real data. The polymorphic information content for each locus was very high (mean value of 0.849), with a total exclusionary power of 0.9995. In summary, seven of the eight microsatellites analysed proved to be sufficient and powerful tools for parentage assignment in hatcheries and the allele frequency data given here can be used to perform pedigree analysis against which real data may be tested.  相似文献   
46.
A study was undertaken to determine the effect of various dietary carbohydrate‐to‐lipid ratios on growth performance, whole‐body composition and tissue lipid content in Senegalese sole (Solea senegalensis) juveniles. Data on the dietary regulation of key hepatic enzymes of the lipogenic and glycolytic pathways (glucose‐6‐phosphate dehydrogenase, G6PD; malic enzyme, ME; fatty acid synthetase, FAS; pyruvate kinase, PK and glucokinase, GK) were also generated. Four isonitrogenous (crude protein: 52% dry matter (DM)) diets were formulated to contain one of two lipid levels (11% and 21% DM). Within each dietary lipid level, the nature of the carbohydrate fraction (raw or extruded peas) was varied. Triplicate groups of 54 sole (initial body weight: 23.6±1.2 g) were grown in recirculated seawater over 67 days. Fish were fed using automated feeders. At the end of the study, whole‐body, liver, viscera and muscle samples were withdrawn for analyses. During the experimental period, the mean fish weight about doubled in all treatments. No significant differences were found in growth performance (ranging from 1.1% to 1.4% body weight day?1) among dietary treatments. High‐fat diets increased whole‐body fat content. Similarly, daily fat gain ranged from 0.54 to 0.78 g kg?1 day?1 and highest values were found in fish fed high‐lipid diets. Dietary treatments also affected tissue lipid content (liver, viscera and muscle), with highest values in fish fed high‐fat diets. The nature of dietary carbohydrates had little influence on performance criteria, but affected tissue lipid deposition. The activities of G6PD, ME and FAS were depressed by elevated levels of dietary lipid, confirming the inhibitory effect of dietary fats on lipid biosynthesis. At both dietary lipid levels, ME and FAS activities were little affected by dietary carbohydrate. Activities of PK and GK were not affected by the starch level of the diets. In Senegalese sole juveniles, the lipogenic pathway is more susceptible to modulation by dietary means (particularly through lipid intake) than the glycolytic pathway.  相似文献   
47.
The autonomous underwater vehicle (AUV) Autosub-2 was deployed on eight missions ahead of RRS James Clark Ross in the northern Weddell Sea and in the Bransfield Strait, Southern Ocean, to assess avoidance of the research vessel by Antarctic krill Euphausia superba. The AUV was equipped with the same type of scientific echosounder as the research vessel (Simrad EK500 operating at 38 and 120 kHz) and measured the density of krill along transect acoustically (g m−2 wet mass) prior to the ship’s arrival. We hypothesised that if krill avoided the ship, perhaps in response to radiated noise, then the ship should detect less krill than the AUV which is known to have much lower noise levels than the ship. We were unable to detect any significant difference between the density of krill detected by the ship or the AUV, either at the transect level or at finer scales within transects. We conclude, therefore, that avoidance by krill of RRS James Clark Ross will not significantly bias acoustic estimates of krill abundance by this vessel.  相似文献   
48.
A feeding experiment was conducted over 9 weeks with seven groups of 30 (fish per group) unpigmented gilthead seabream, Sparus aurata (L. 1875) (initial mean weight = 145.2 ± 12.3 g). Three experimental diets were prepared by adding to a basal diet free of carotenoid (final pigment content of around 40 mg per kg feed): (i) a biomass of the carotenogenic Chlorella vulgaris (Chlorophyta, Volvocales); (ii) a synthetic astaxanthin; and (iii) a mixture (1:1) of microalgal biomass and synthetic astaxanthin. At 3‐week intervals, five fish were sampled from each tank for total carotenoids analysis in skin and muscle. The carotenoid pigments (total amount = 0.4%) identified in the carotenogenic alga were lutein (0.3%), β‐carotene (1.2%), canthaxanthin (36.2%), astaxanthin, free and esterified forms (55.0%), and other pigments (7.3%). Carotenoid pigments were significantly deposited in the four skin zones studied during the feeding trial: the forefront between the eyes, the opercule, along the dorsal fin and in the abdominal area. In the muscle, regardless of the astaxanthin source, the amount of carotenoids measured was very low (less than 1 mg kg?1) and differences not significant. Moreover, no muscle pigmentation was evident, and there was no variation in the amount of carotenoid analysed in skin tissue, through the trial, for each treatment. It was concluded that supplementing the feed with C. vulgaris would be an acceptable practice in aquaculture to improve the market appeal of the gilthead seabream.  相似文献   
49.
Feeding experiments were conducted on large and small adult freshwater prawns Macrobrachium rosenbergii (36.96 ± 1.65 and 13.48 ± 1.65 g in weight) to determine the impact of dietary fiber on growth, digestion, and gastric emptying time. Fiber was incorporated as Q- cellulose into four semi-purified diets at calculated levels of 0, 5, 10 and 15% at the expense of starch and fed for an 8-wk period. The experiment was conducted in a closed, filtered, recirculating freshwater system. The results indicate that the specific growth rates, feed conversion ratios, and protein efficiency ratios improved as the levels of dietary fiber increased from 0.4% to 8%. Gastric emptying times increased as the level of inclusion of cellulose in the diets increased. It was concluded that diets containing up to 10% dietary fiber increases growth rates in adult prawn Macrobrachium rosenbergii by increasing nutrient residence time in the gastrointestinal tract, thereby increasing absorption.  相似文献   
50.
Meat production by goats has become an important livestock enterprise in several parts of the world. Nonetheless, energy and protein requirements of meat goats have not been defined thoroughly. The objective of this study was to determine the energy and protein requirements for maintenance and growth of 34 (3/4) Boer x (1/4) Saanen crossbred, intact male kids (20.5 +/- 0.24 kg of initial BW). The baseline group was 7 randomly selected kids, averaging 21.2 +/- 0.36 kg of BW. An intermediate group consisted of 6 randomly selected kids, fed for ad libitum intake, that were slaughtered when they reached an average BW of 28.2 +/- 0.39 kg. The remaining kids (n = 21) were allocated randomly on d 0 to 3 levels of DMI (treatments were ad libitum or restricted to 70 or 40% of the ad libitum intake) within 7 slaughter groups. A slaughter group contained 1 kid from each treatment, and kids were slaughtered when the ad libitum treatment kid reached 35 kg of BW. Individual body components (head plus feet, hide, internal organs plus blood, and carcass) were weighed, ground, mixed, and subsampled for chemical analyses. Initial body composition was determined using equations developed from the composition of the baseline kids. The calculated daily maintenance requirement for NE was 77.3 +/- 1.05 kcal/kg(0.75) of empty BW (EBW) or 67.4 +/- 1.04 kcal/kg(0.75) of shrunk BW. The daily ME requirement for maintenance (118.1 kcal/kg(0.75) of EBW or 103.0 kcal/kg(0.75) of shrunk BW) was calculated by iteration, assuming that the heat produced was equal to the ME intake at maintenance. The partial efficiency of use of ME for NE below maintenance was 0.65. A value of 2.44 +/- 0.4 g of net protein/kg(0.75) of EBW for daily maintenance was determined. Net energy requirements for growth ranged from 2.55 to 3.0 Mcal/kg of EBW gain at 20 and 35 kg of BW, and net protein requirements for growth ranged from 178.8 to 185.2 g/kg of EBW gain. These results suggest that NE and net protein requirements for growing meat goats exceed the requirements previously published for dairy goats. Moreover, results from this study suggest that the N requirement for maintenance for growing goats is greater than the established recommendations.  相似文献   
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