Effect of pearl millet silage ammoniated with urea on lamb production and metabolic performance          下载免费PDF全文

G. G. P. Carvalho  P. M. D. Freitas  E. M. Santos  G. G. L. Araújo  J. S. Oliveira  A. J. V. Pires  C. M. A. Maranhão  T. C. G. C. Rodrigues  J. E. Freitas Júnior  L. M. A. Rufino  C. S. Rodrigues  L. C. Leite  M. L. G. M. L. Araujo 《Grass and Forage Science》2018,73(3):685-693

The objective of this study was to determine the effect of diets containing pearl millet silage ammoniated with urea on the intake, digestibility, production performance, and nitrogen metabolism of lambs. Thirty‐two uncastrated mixed‐breed lambs at 4 to 5 months of age, with an average initial body weight of 17.39 ± 2.16 kg, were distributed into four treatments in a randomized block experimental design with eight replicates. Experimental diets consisted of pearl millet silage ammoniated with urea during ensiling at the levels of 0, 20, 40, and 60 g/kg dry matter (DM). Increasing urea levels in the pearl millet silage led to a linear decrease (p < .05) in the intakes of DM, organic matter, and total digestible nutrients by the lambs. Total and average daily weight gains decreased linearly (p < .05) with the addition of urea to the silage. Intake and digestibility of crude protein, nitrogen intake, urine urea nitrogen, plasma urea nitrogen, microbial synthesis, and microbial efficiency had a quadratic response (p < .05) to the urea levels in the silage. Pearl millet silage ammoniated with urea reduces dry‐matter intake and daily weight gain in lambs. Urea is not recommended for use in the ensiling of pearl millet.  相似文献   

Variable rate spraying application on cotton using an electronic flow controller     

F.?H.?R.?BaioEmail author  D.?C.?Neves  H.?B.?Souza  A.?J.?F.?Leal  R.?C.?Leite  J.?P.?Molin  S.?P.?Silva 《Precision Agriculture》2018,19(5):912-928

Vegetation indices (VI) obtained by optical sensors have a positive correlation with various attributes of cotton plant growth. This work is aimed at evaluating the variable rate application of plant growth regulator (PGR) and fruit ripener on zones defined by VI and penological measurements using a sprayer equipped with a relatively low cost electronic flow controller on the height, percentage of open fruits, yield and net income. The work was done in a 92 ha field during crop seasons 2012/2013 and 2013/2014, and in a 202 ha field, during the crop season 2014/2015. Two spray applications were made using variable rate technology (VRT) of the PGR and one fruit ripener, in both harvest seasons, according to three VI classes formed by a previous mapping. The uniformity of the cotton height and opened fruits contribute to a similar yield across zones. Uniform plant height facilitates cotton harvest. The ripener helps to ensure all the cotton is ready to be harvested at the same time. In this trial, use of VRT technique to manage the PGR and fruit ripener application increased net income by US$152.28 ha^?1, but this estimate is based on yields that are not statistically significantly different from the control. This research confirms that PGR and fruit ripener can be sufficiently managed with an electronic flow controller to result in more uniform cotton plant height and yields within fields, but it leaves open the question of whether VRT PGR is profitable even with the lower cost electronic flow controller.  相似文献   

A Labelled Avidin–Biotin ELISA to Detect Antibodies to Caprine Arthritis-encephalitis Virus in Goats' Sera     

Castro RS  Leite RC  Resende M  Gouveia AM 《Veterinary research communications》1999,23(8):515-522

A labelled avidin–biotin ELISA (lab-ELISA) was developed and compared with indirect ELISA (i-ELISA) and agar-gel immunodiffusion assay (AGID) for its efficacy in detecting antibodies against caprine arthritis-encephalitis virus (CAEV) in goat sera. The enzyme immunoassays were standardized using 113 sera from CAEV-negative goat flocks. The tests were compared using the results from 339 serum samples. The lab-ELISA showed the greatest number of positive results (94/339) as compared with AGID (51) and i-ELISA (64). The comparison of the other two tests with the lab-ELISA showed an agreement of 87.3% with AGID and 90.6% with i-ELISA. The lab-ELISA may be useful for screening large populations for CAEV antibodies, in epidemiological surveys and in the control of caprine arthritis-encephalitis.  相似文献   

Culture methods differ on the isolation of Salmonella enterica serotypes from naturally contaminated swine fecal samples.     

Marcos H Rostagno  Jared K Gailey  H Scott Hurd  James D Mckean  Romulo C Leite 《Journal of veterinary diagnostic investigation》2005,17(1):80-83

Four culture methods (A, B, C, and D) were comparatively evaluated for their ability to isolate Salmonella enterica from pooled swine fecal samples (n = 100). None of the methods was able to isolate Salmonella from all positive samples. The relative sensitivity of the culture methods evaluated was 82%, 94%, 95%, and 78% for methods A, B, C, and D, respectively. The comparison of sensitivities showed that methods B and C performed significantly better (P < 0.05) than methods A and D. Although relative sensitivities of methods B and C were equal, from the 89 positive samples concomitantly detected by both, 35 (39.3%) had different serotypes (no match) isolated by each method. On the basis of the results of this study, it was concluded that culture methods differ on the isolation of S. enterica serotypes from naturally contaminated swine fecal samples. Depending on the objective(s) of investigations on the ecology and epidemiology of S. enterica in swine populations, a method or a combination of methods should be considered for more reliable results.  相似文献   

Relationships of disease and leaf area variables with yield in the Alternaria helianthi–sunflower pathosystem     

R. M. V. B. C. Leite  L. Amorim  A. Bergamin Filho 《Plant pathology》2006,55(1):73-81

  相似文献   

Oral focal epithelial hyperplasia in a howler monkey (Alouatta fusca)     

Sá LR  DiLoreto C  Leite MC  Wakamatsu A  Santos RT  Catão-Dias JL 《Veterinary pathology》2000,37(5):492-496

  相似文献   

Purification and characterization of the fimbria F18ac (2134P) isolated from enterotoxigenic Escherichia coli (ETEC)   总被引：10，自引：0，他引：10  

Amorim CR  Matsuura MS  Rosa JC  Greene LJ  Leite DS  Yano T 《Veterinary microbiology》2000,76(1):41-49

The adhesin F18ac purified on Sepharose CL 4B column chromatography and SDS-PAGE stained with Coomassie Blue and Western blotting using specific anti-F18ac serum presented one band of approximately 17kDa. Gold immunolabeling revealed that the adhesin F18ac has a fimbrial structure on the bacterial surface. The first 27 amino acid residues of the N-terminal portion of the adhesin F18ac, showed 92.5% homology (25 amino acids) with the F107 (F18ab) fimbriae.  相似文献   

Spatial distribution patterns of riverine forest taxa in Brasília, Brazil     

Edson J. Leite 《Forest Ecology and Management》2001,140(2-3):257-264

The spatial distribution of adult individuals (≥15 cm DBH) was investigated in a gallery forest of the Brasília National Park, Brazil. Four wood species, important to the Brazilian national genetic conservation programme, were chosen: Astronium fraxinifolium Schott, (Anacardiaceae); Cariniana estrellensis (Raddi) Kuntze, (Lecythidaceae); Didymopanax morototoni (Aublet) Decne. & Planch., (Araliaceae) and Hymenaea courbaril var. stilbocarpa (Hayne) Lee & Lang., (Leguminosae-Caesalpinioideae). While distribution was uneven, no relationship with gradient or togographic position could be detected, but aggregation was perceived. Stocking levels were typically low, from 0.15 to 3.31 individuals ha⁻¹.  相似文献   

Responses of eucalypt species to aluminum: the possible involvement of low molecular weight organic acids in the Al tolerance mechanism     

Silva IR  Novais RF  Jham GN  Barros NF  Gebrim FO  Nunes FN  Neves JC  Leite FP 《Tree physiology》2004,24(11):1267-1277

Aluminum (Al) tolerance mechanisms in crop plants have been extensively researched, but our understanding of the physiological mechanisms underlying Al tolerance in trees is still limited. To investigate Al tolerance in eucalypts, seedlings of six species (Eucalyptus globulus Labill., Eucalyptus urophylla S.T. Blake, Eucalyptus dunnii Maiden, Eucalyptus saligna Sm., Eucalyptus cloeziana F. J. Muell. and Eucalyptus grandis w. Hill ex Maiden) and seedlings of six clones of Eucalyptus species were grown for 10 days in nutrient solutions containing Al concentrations varying from 0 to 2.5 microM (0 to 648 microM Al3+ activities). Root elongation of most species was inhibited only by high Al3+ activities. Low to intermediate Al3+ activities were beneficial to root elongation of all species and clones. Among the species tested, E. globulus and E. urophylla were more tolerant to Al toxicity, whereas E. grandis and E. cloeziana were more susceptible to Al-induced damage. Although E. globulus seedlings were tolerant to Al toxicity, they were highly sensitive to lanthanum (La), indicating that the tolerance mechanism is specific for Al. Fine roots accumulated more Al and their elongation was inhibited more than that of thick roots. In E. globulus, accumulation of Al in root tips increased linearly with increasing Al concentration in the nutrient solution. The majority of Al taken up was retained in the root system, and the small amounts of Al translocated to the shoot system were found mainly in older leaves. No more than 60% of the Al in the thick root tip was in an exchangeable form in the apoplast that could be removed by sequential citrate rinses. Gas chromatography/mass spectrometry and ion chromatography analyses indicated that root exposure to Al led to a greater than 200% increase in malic acid concentration in the root tips of all eucalypt species. The increase in malate concentration in response to Al treatment correlated with the degree of Al tolerance of the species. A small increase in citric acid concentration was also observed in all species, but there were no consistent changes in the concentrations of other organic acids in response to Al treatment. In all eucalypt species, Al treatment induced the secretion of citric and malic acid in root exudates, but no trend with respect to Al tolerance was observed. Thus, although malate and citrate exudation by roots may partially account for the overall high Al tolerance of these eucalypt species, it appears that tolerance is mainly derived from the internal detoxification of Al by complexation with malic acid.  相似文献   

David and Goliath in a dog's blood          下载免费PDF全文

Ricardo Marcos  Marta Santos  Liliana Leite‐Martins  Miguel França  Augusto de Matos 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(4):475-476

  相似文献