Comparative study of the microbial profile from bilateral canine otitis externa     

Oliveira LC  Leite CA  Brilhante RS  Carvalho CB 《The Canadian veterinary journal. La revue veterinaire canadienne》2008,49(8):785-788

Fifty dogs with bilateral otitis externa were studied over a 10-month period. The exudates of both external ears were obtained, using sterile swabs, and microorganisms were isolated according to standard microbiological techniques. Antimicrobial susceptibility testing of Staphylococcus intermedius was done by the agar diffusion method. There was bacterial and/or fungal growth in all of the samples. These were all polymicrobial infections. Anaerobic bacteria were not isolated in any sample. The most common pathogens isolated were S. intermedius and Malassezia pachydermatis. A statistically significant difference (P < 0.05) was observed in the isolation pattern between the right and left ears in 34 of the 50 animals (68%). High resistance rates of S. intermedius strains to penicillin, ampicillin, erythromycin, tetracycline, and clindamycin were found. The results suggest that in bilateral canine otitis externa, each ear should be cultured separately and considered as separate units.  相似文献   

Lunasin and Bowman-Birk protease inhibitor concentrations of protein extracts from enzyme-assisted aqueous extraction of soybeans     

Leite Nobrega de Moura JM  Hernandez-Ledesma B  de Almeida NM  Hsieh CC  de Lumen BO  Johnson LA 《Journal of agricultural and food chemistry》2011,59(13):6940-6946

Lunasin and Bowman-Birk protease inhibitor (BBI) are two soybean peptides to which health-promoting properties have been attributed. Concentrations of these peptides were determined in skim fractions produced by enzyme-assisted aqueous extraction processing (EAEP) of extruded full-fat soybean flakes (an alternative to extracting oil from soybeans with hexane) and compared with similar extracts from hexane-defatted soybean meal. Oil and protein were extracted by using countercurrent two-stage EAEP of soybeans at 1:6 solids-to-liquid ratio, 50 °C, pH 9.0, and 120 rpm for 1 h. Protein-rich skim fractions were produced from extruded full-fat soybean flakes using different enzyme strategies in EAEP: 0.5% protease (wt/g extruded flakes) used in both extraction stages; 0.5% protease used only in the second extraction stage; no enzyme used in either extraction stage. Countercurrent two-stage protein extraction of air-desolventized, hexane-defatted soybean flakes was used as a control. Protein extraction yields increased from 66% to 89-96% when using countercurrent two-stage EAEP with extruded full-fat flakes compared to 85% when using countercurrent two-stage protein extraction of air-desolventized, hexane-defatted soybean flakes. Extruding full-fat soybean flakes reduced BBI activity. Enzymatic hydrolysis reduced BBI contents of EAEP skims. Lunasin, however, was more resistant to both enzymatic hydrolysis and heat denaturation. Although using enzymes in both EAEP extraction stages yielded the highest protein and oil extractions, reducing enzyme use to only the second stage preserved much of the BBI and Lunasin.  相似文献   

Note: Flight capacity,parasitism and emergence of five<Emphasis Type="Italic">Trichogramma</Emphasis> (Hymenoptera: Trichogrammatidae) species from forest areas in Brazil     

M. A. Soares  G. L. D. Leite  J. C. Zanuncio  S. L. Rocha  V. G. M. de Sá  J. E. Serrão 《Phytoparasitica》2007,35(3):314-318

The flight and parasitism capacities and emergence rate of fiveTrichogramma species [T. acacioi (Brun),T. bruni (Nagaraja),T. demoraesi (Nagaraja),T. maxacalii (Voegelé & Pointel) andT. soaresi (Nagaraja)] were compared. The flight capacity of these parasitoids was assessed in test units. A tube was placed inside each test unit with a piece of card with 300Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) eggs parasitized by one of theTrichogramma species. The emerged adults inside the test units were classified according to their position as flyers, non-flyers and walkers. Cards withA. kuehniella eggs, parasitized by one of theTrichogramma species, were assessed to determine the parasitism rate and emergence of these natural enemies. The data were submitted to analysis of variance and the means compared by the Scott-Knott test at 5% probability.Trichogramma maxacalii exhibited better flight capacity than the other species, in addition to good parasitism rate and high percentage of emergence. http://www.phytoparasitica.org posting May 10, 2007.  相似文献   

Parasitic helminth infection in young cattle raised on silvopasture and open-pasture in Southeastern Brazil     

R. M. A. de Mendonca  R. C. Leite  A. M. Q. Lana  J. O. Costa  G. Toth 《Agroforestry Systems》2014,88(1):53-62

More than 95 % of Brazil’s beef production occurs in pastures where parasitic helminths are a serious problem, potentially inhibiting an average annual weight gain of 8 kg per young animal. Current pharmaceutical treatments for parasitic infestation are expensive (estimated to cost Brazil 370 million U.S. dollars per year) and sometimes inefficient due to rapid adaptation of worms to new drugs. The presence of trees in pastures (silvopasture) has proven to be environmentally beneficial, but may favor multiplication of parasites and cause higher infestation rates compared to traditional pastures. This study in Southeastern Brazil compared the weight gains of crossbred Holstein and Gir (Zebu) bovines, 8–24 months of age, under two different grazing systems: open (tree-less) pasture, and silvopasture (primarily “Sucupiras Brancas” at 156 trees ha^?1). Despite the favorable environment for helminths, silvopasture did not increase the worm egg count per gram of animal feces and did not create overall weight or weight gain patterns different from those of animals grazing on the open pasture. The experiment took place from August 2008 to February 2009, encapsulating the transition from the dry to rainy season; a period when worm infection rates are highest, causing the greatest impact in animal development. The significance of this period aside, the results must be taken as indicative due to the short time frame and the extraneous challenges of climate variation, individual immune system resistance development, and natural helminth predator growth conditions.  相似文献   

Effects of submergence in water on seed germination and vigor of the Copaifera lucens （Fabaceae） seedlings     

Daniela Baldez Vidal  ;Isis Leite Andrade  ;Eusinia Louzada Pereira Andrade  ;Marcelo Schramm Mielke 《林业研究》2014,25(4):903-908

We analyzed the tolerance of Copaifera lucens seeds to sub- mersion in water to assess the use of this species for direct seeding in riparian forest restoration programs. Seeds were submerged in water for 2, 4, 8, 16 and 32 days or not submerged （control = 0 days of submer- gence）. For the control and at the end of each period of submersion, germination and seedling vigor tests were carried out. For germination tests, seeds were sown in plastic pots containing sand and kept in labora- tory conditions. The percentage of seed germination, the germination rate and the average germination time were analyzed. For seedlings, total biomass, leaf area, leaf mass per area and leaf area ratio were analyzed. Submersion time drastically affected the dissolved oxygen content and seed germination. Between 4 and 8 days of submersion there was a de- crease from 83.8% to 15.6% in the germination percentage. No seed germination occurred after 16 days of submersion. Although there was a significant decrease in the percentage of seed germination between 4 and 8 days of submersion, seedling vigor was not affected. Seeds of this species were partially tolerant to submersion in water, suggesting that C. lucens is a promising species for direct seeding in riparian forest restora- tion projects.  相似文献   

Histomorphometrical study on the skin of different regions of the Caribbean common opossum (Didelphis marsupialis Linnaeus, 1758)     

Reda Mohamed  William Pérez  Marcelo Leite da Veiga 《Anatomia, histologia, embryologia》2021,50(1):144-150

  相似文献   

Tantalum Dissolution Rate in Simulant Lung Fluid     

C. Lima  M. J. Coelho  K. C. P. Dalia  C. V. Barros Leite  G. Medeiros  K. Dias da Cunha 《Water, air, and soil pollution》2007,186(1-4):365-371

The aim of this study is to determine the solubility rate of tantalum contained in pyrochlore, columbite–tantalite and columbite in simulant lung fluid. The International Commission on Radiological Protection (ICRP), in its recommendation for limiting intakes of radionuclide by workers, has consistently recognized that the biological behavior of any specific material after incorporation can significantly diverge from model prevision. Model parameters should be adjusted to adapt the model for each specific substance material in order to estimate the dose due to this element intake. The most recent ICRP publication 66, respiratory tract dosimetry model, point out that information as data like particle size, aerosols solubility and the material chemical compounds are important parameters in the dose coefficients calculation. This paper studies the solubility in Simulant Lung Fluid (SLF) of Ta present in mineral dust particles. For this study 3 minerals were selected: pyrochlore, columbite–tantalite and columbite. Tantalum dissolution in vitro samples were obtained using the Gamble solution and PIXE (Particle Induced X-ray Emission) as analytical technique. In order to characterize the worker exposure to Ta bearing particles, one Brazilian niobium mine was selected. The mineral dust particles were collected using a six-stage cascade impactor and the elemental mass concentrations and the MMAD (Mass Median Aerodynamic Diameter) were determined. Concentrations of radionuclides from natural series of thorium and uranium were also determined. The results show that the workers are exposed to Ta bearing particles in the respirable fraction of aerosols (aerodynamic diameter (d _aer) <2.5 μm) during the mineral processing to obtain Fe–Nb alloy. The solubility in Simulant Lung Fluid (SLF) of Ta present in mineral dust particles depends on the mineral characteristics. The solubility half-time varies between 34 and 62 h depending on the associated mineral.  相似文献   

Shading effect on physiological parameters and in vitro embryo production of tropical adapted Nellore heifers in integrated crop-livestock-forest systems     

Leite da Silva  Wilian Aparecido  Poehland  Ralf  Carvalho de Oliveira  Caroline  Ribeiro Ferreira  Mariane Gabriela Cesar  Garcia de Almeida  Ricardo  Cáceres  Mirela Brochado Souza  Macedo  Gustavo Guerino  da Costa e Silva  Eliane Vianna  Alves  Fabiana Villa  Nogueira  Eriklis  de Andrade Melo-Sterza  Fabiana 《Tropical animal health and production》2020,52(5):2273-2281

Tropical Animal Health and Production - The aim of this study was to evaluate the impact of increased shadow supply in integrated crop-livestock-forest systems on in vitro embryonic development and...  相似文献   

Spatial patterns of magnetic susceptibility optimized by anisotropic correction in different Alisols in southern Amazonas,Brazil     

Brito  Wildson Benedito Mendes  Campos  Milton César Costa  de Souza  Fernando Gomes  Silva  Laércio Santos  da Cunha  José Maurício  de Lima  Alan Ferreira Leite  Martins  Thalita Silva  de Oliveira  Flávio Pereira  de Oliveira  Ivanildo Amorim 《Precision Agriculture》2022,23(2):419-449

Precision Agriculture - Changes in primary cover for agricultural crops in Amazonas region influence the phenomenon of spatial variability in soil properties. This phenomenon is still studied...  相似文献   

Sun coral management effectiveness in a wildlife refuge from south-eastern Brazil     

Lucca A. C. Savio  Gustavo M. Dias  Kelen L. Leite  Silvia N. Godoi  Apoena C. Figueiroa  Geraldo F. O. Neto  Edineia C. Correa  Carlo L. B. Francini  Gustavo Shintate  Marcelo V. Kitahara 《水产资源保护:海洋与淡水生态系统》2021,31(10):2830-2841

1. The introduction of exotic species is one of the major causes for biodiversity loss in marine environments. Introduced species can change habitat complexity, impacting local communities and altering ecosystem functioning.
2. Since the 1980s, the scleractinian species Tubastraea coccinea and Tubastraea. tagusensis (commonly known as sun corals) have been spreading in the south-western Atlantic, posing a major threat to local biodiversity and marine ecosystems. Nowadays, these species are reported from more than 3,500 km along the Brazilian coast and on at least 21 oil and gas offshore platforms.
3. Considering the great environmental pressure resulting from sun coral invasion, this study verified the effectiveness of manual removal of Tubastraea spp. along a rocky shore in Alcatrazes Wildlife Refuge, one of the most pristine marine regions in Brazil.
4. Results indicate that removal is indeed effective as a control technique; however, as invaders have high regeneration capabilities and recruitment rates, successive management efforts are essential. In order for management to be effective against Tubastraea's year-round release of clonal larvae, results suggest invaded areas should receive at least two management actions per year.
5. Successive management efforts, in addition to the development of new techniques and prevention of new invasions should be priorities for mitigating sun coral impacts.

  相似文献