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191.
Metabolic and production responses are reported for 72 cows treated with bovine somatotropin (BST) for 30 days starting at day 70 of lactation. Of these 72 cows, 48 had been exposed in the preceding lactation to long-term treatment with BST at 3 dosages and 24 (controls) had not been given BST. Approximately half of the cows in each group were parity-2 cows, the rest were older. Comparisons between groups were made separately for parity-2, and older cows. Analyses, using pretreatment values of each variable as a covariate, indicated that older cows, but not parity-2 cows, significantly (P less than 0.05) increased milk production during treatment. Parity-2 cows, however, had a significantly higher milk fat percentage than controls following treatment. Cows treated with 51.6 or 86 mg BST/d in both parity groups had significantly higher serum-free fatty acids than controls. Estimated net energy balances were significantly lower for older treated cows, but did not significantly differ from controls for parity-2 treated cows. Older cows in the 86 mg of BST/d group tended to have higher concentrations of blood glucose than did older control-group cows. Treatment with BST did not significantly increase serum ketone concentrations in any group of animals, and none of the cows developed clinical ketosis during this period. Estimated net energy balance (ENEB) during treatment was a significant (P less than 0.05) covariate for free fatty acid concentrations in older cows and for milk fat percentage in parity-2 cows. Covariate adjusted analyses, using ENEB during treatment as a covariate, indicated that lipolytic stimuli already acting may be enhanced by treatment with BST, but a negative energy balance was not a necessary precondition for free fatty acid concentrations to increase following somatotropin treatment. Similarly, milk fat percentages for parity-2 treated cows were significantly (P less than 0.05) higher during treatment than controls when ENEB during treatment was used as a covariate. Increased milk fat concentrations in parity-2 treated cows were not associated with significant increases in the ratio of C18:C4-10 milk fatty acids, indicating that increased milk fat resulted from either an increase in incorporation of C18 fatty acids into milk fat coupled with an increase in de novo mammary synthesis of C4-10 milk fatty acids or an increase in C12-16 fatty acids that may arise either from increased tissue mobilization, from diet, or from de novo mammary synthesis.  相似文献   
192.
Osteolytic defects were detected radiographically in the distal sesamoid bone of a 16-month-old Bralers heifer, in the middle phalanx of a 14-month-old American Gray Brahman bull, and in the distal phalanx of a 3-year-old American Gray Brahman bull. The articular cartilage was damaged in each animal because of osteolysis or pathologic fracture. After each animal was anesthetized and positioned in lateral recumbency, the lesions were curetted and packed with cancellous bone harvested from the same animal's tuber coxae. Basic postoperative management involved stall rest and immobilization of the graft site with a fiberglass cast (42 to 79 days), after which a support bandage was used for approximately 2 weeks. Recurrence of lameness has not been observed in these animals for 60 months, 58 months, and 21 months, respectively. These cases exemplify the benefit of using an autogenous cancellous bone graft for treatment of severe osteolysis of a digit in cattle.  相似文献   
193.
A Campylobacter jejuni isolate obtained from a turkey liver, designated C101, and a C. jejuni isolate obtained from the feces of a chicken, designated C111, were used to inoculate their respective hosts. Isolate C101 depressed weight gain by 20% when inoculated into newly hatched poults or 4-day-old poults. It also caused death, hepatic necrosis, and generalized hemorrhages in turkey embryos. The chicken-derived isolate, C111, did not reduce weight gain in newly hatched chicks, but it did induce mortality in chicken embryos. The supernatant of the cultures of both C. jejuni isolates also caused mortality in embryos.  相似文献   
194.
Dynamics of the cumulation of phosphorus, potassium, calcium and magnesium in developing seed of four soybean genotypes under the defined climatic conditions in the course of three years was observed. Samples were being taken at weekly intervals from the point at which the grain reached a size suitable to be analyzed up to the full maturity of the seed. By analyzing the results it was found out that the intense cumulation of the minerals tested took place during the stages III and IV of the seed development, that is, in the course of intense cumulation of dry matter and the most expressive metabolic activity in the seed. Investigation of the impact of climatic conditions provides evidence that cumulation of the tested macroelements has been favourably influenced by increased precipitation along with moderate air temperatures within the seed developing period. The highest content of the minerals found with the cultivar Maksimirka independently of the year investigated reveals that mineral cumulation in soybean seed is affected by the cultivar and genetic particularity.  相似文献   
195.
The response time to cimaterol (CIM), a beta-adrenergic agonist, by broiler chickens for carcass characteristics, muscle composition, muscle fiber size, catheptic enzyme activity, and tenderness was determined. Two trials were conducted in which chickens were fed a control diet (CON) containing 0 ppm of CIM or a diet containing 1 ppm of CIM. Trial 1 consisted of 55, 31-d-old broiler chickens individually fed for up to 48 h. At 0, 6, 12, 18, 24, and 48 h, five CON and five CIM-fed chickens were killed. Trial 2 consisted of 160, 33-d-old broiler chickens group-fed for up to 14 d. At 2, 4, 6, 8, 10, 12, and 14 d, 10 CON and 10 CIM-fed chickens were killed. The breast muscle (BM) and leg muscle (LM) weight, cathepsin B and L activities, DNA, RNA, and protein concentration, and BM shear force value (SFV) were measured in both trials. Thigh muscle (TM) SFV were measured in Trial 2 only. Fiber size of BM was measured (five birds per treatment) at d 2, 6, 10, and 14. In Trial 1, BM weight and SFV were lower in CIM-fed birds at 6 h (P less than .05). In Trial 2 BM SFV were higher at d 8 (P = .06) and d 10 (P less than .05) in CIM-fed chickens. The SFV of CIM-fed chickens were higher at d 4, 8, 10, 12, and 14 (P less than .05).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   
196.
Lean tissue growth rate is usually estimated from indirect measurements including growth rate. A procedure to determine prediction equations for lean tissue growth rate is proposed. The procedure restricts the regression of fat growth rate on predicted lean growth rate to be equal to the regression of fat growth rate on actual lean growth rate. The restriction can be phenotypic or genetic if suitable parameter estimates are available. When applied phenotypically, selection on predicted lean tissue growth rate will result in selection differentials for both fat and lean tissue growth rates that are proportional to those obtained by direct selection for lean tissue growth rate. This restriction is desirable because expected correlated changes in fat are used to justify selection for lean tissue growth. Conventional prediction procedures have ignored correlated changes and obscured the original intent of using lean tissue growth rate as a biological selection criterion. When using conventional procedures to predict a biological selection criterion from indirect measurements, changes in important correlated traits may depend more on the choice of indirect measurements than on the choice of selection criterion.  相似文献   
197.
A 6-year study was conducted in 4 dairy herds in Iowa in which Johne's disease was diagnosed previously. Fecal specimens were collected at 6-month intervals from animals 2 years of age and over for mycobacteriologic examination. Serum samples were obtained at 3-month intervals and tested by enzyme-linked immunosorbent assay (ELISA). The antigen used in the ELISA was a potassium chloride extract of a field strain of Mycobacterium paratuberculosis. The ELISA reactions were observed in 87% of the cows from which M. paratuberculosis was isolated. Dairy producers that participated in the Johne's control program reported reduced economic losses. Increased income was attributed to improved milk production, increased value of vaccinated animals sold as replacements to other dairy herds in which Johne's disease had been diagnosed, and the increased market value of slaughter animals removed from the herd.  相似文献   
198.
An ultrasonic linear array scanner with a transrectal probe was used to observe ovarian and uterine changes associated with the reproductive cycle in llamas. 'Waves' of follicular development and regression occurred in unstimulated females, during which the dominant follicle reached a maximum size of 9 to 13 mm; both ovaries were equally active. Ovulation was induced by mating in 80 per cent of cases, and when mating was accompanied by the administration of human chorionic gonadotrophin the ovulation rate increased to 90 per cent and the time to ovulation decreased from two to three days to one to two days. Some spontaneous ovulations occurred. Corpora lutea reached a maximum size of 12 mm (non-pregnant) or 14 mm (pregnant) after seven or 16 days, respectively. The lifespan of the corpus luteum was approximately 11 days in non-pregnant llamas and the regression time was advanced by the administration of prostaglandin or embryo recovery. Pregnancy could be diagnosed as early as 19 days after mating.  相似文献   
199.
Tooth root and root trunk surface area measurements were taken from 20 first mandibular molar (M1) teeth obtained randomly from canine cadaver specimens. The mean root surface area for all teeth was 497.1 +/- 116.2 mm2. The cumulative surface areas of the mesial (MR) and distal (DR) roots were 251.4 +/- 70.2 mm2 and 211.7 +/- 51.9 mm2, respectively. The root trunk surface area was 34.0 +/- 21.4 mm2. Surface area measurements for individual roots and the root trunk were significantly different. Cumulative individual root surface area (MR + DR) was 93.2% of total root surface area. The MR surface area contributed most to total root surface area (50.6%) and cumulative individual root surface area (54.3%). The mean distance from the beginning of the root trunk to the furcation was 2.1 mm. On the basis of the linear variation of the percentage of root surface area of the M1, 44.6% of the total root surface area was 3 to 7 mm apical to the most coronal aspect of the cementoenamel junction. The MR was heart-shaped, compared with the more cylindrical shape of the DR.  相似文献   
200.
The persistence and mobility of chlorsulfuron and metsulfuron were studied in Italy at four field locations. Chlorsulfuron was applied pre-emer-gence to wheat at 15 and 30 g a.i. ha?1 in the autumn of 1986 and metsulfuron early post-emergence at 8 g a.i. ha?1 in the spring of 1987. Soil samples were taken periodically at each location for 1 year. Trials were conducted until 1989 at three locations. After the wheat harvest in 1989, chlorsulfuron and metsulfuron residues in the soil were measured by bioassay in plots that had been treated annually for 3 years and also in plots that had received single treatments. Chlorsulfuron at both application rates dissipated to levels below 0.3 g a.i. ha?1 over 12 months at three locations while, at the fourth, which received only 250 mm rain during the study, significant levels of residues were still present after 15 months. The degradation of chlorsulfuron applied at 30 g a.i. ha?1 followed first-order kinetics, with half-lives of 149, 70, 59 and 51 days at the four locations. Chlorsulfuron mobility was high at three locations while, in the silty clay soil of the fourth, the herbicide was confined to the upper 30 cm, despite the high rainfall. Metsulfuron applied in spring was detected only in the first soil sample collection. It was present in all sampled layers at one location but, in the others, was confined to the surface layer. There was no evidence of herbicide accumulation in the soil with repeated applications. Persistance et mobilité du chlorsulfuron et du met-sulfuron dans différents sols et sous différentes conditions climatiques La persistance et la mobilité du chorsulfuron et du metsulfuron au champ ont étéétudiées en Italie sur quatre sites. Le chlorsulfuron était appliqué en pré-levée sur le blé aux doses de matière active (m.a.) 15 et 30 g ha?1à l'automne 1986 et le metsulfuron en post-levée précoce à la dose 8 g m.a. ha?1 au printemps 1987. Des échantillons de sol étaient prélevés périodiquement sur chaque site pendant 1 an. Les essais ont été conduits jusqu'en 1989 sur trois des sites. En 1989, après la récolte du blé. les résidus de chlorsulfuron et de metsulfuron dans le sol ont été mesurés par des essais biologiques dans des parcelles qui avaient été traitées chaque année pendant 3 ans, ainsi que dans des parcelles qui n'avaient reçu qu'un seul traitement. Le chlorsulfuron aux deux doses disparaissait jusqu'à des niveaux inférieurs à 0.3 g m.a. ha?1 en 12 mois sur trois des sites, alors que sur le quatrième, qui n'a reçu que 250 mm de précipitations pendant l'étude, des résidus étaient présents en quantité significative après 15 mois. La dégradation du chlorsulfuron appliquéà la dose 30 g m.a. ha?1 suivait une cinétique du premier ordre, avec des demi-vies 149, 70, 59 et 51 j sur les quatre sites. La mobilité du chlorsulfuron était élevée sur trois des sites alors que sur le quatrième oú le sol était une argile limoneuse, l'herbicide était confiné aux 30 cm superficiels en dépit de précipitations élevées. Le metsulfuron appliqué au printemps n'était détecté que dans le premier prélèvement. Sur un des sites, il était présent dans toutes les couches, mais était confinéà la couche superficielle sur les autres sites. Après des applications répétées, aucune accumulation d'herbicide n'était observée dans le sol. Persistenz und Mobilität von Chlorsulfuron und Metsulfuron in verschiedenen Böden und bei unterschiedlicher Witterung Die Persistenz und Mobilität von Chlorsulfuron und Metsulfuron wurden in Italien an 4 Standorten unter Freilandbedingungen untersucht. Chlorsulfuron wurde im Herbst 1986 bei Weizen im Vorauflauf mit 15 und 30 g AS ha?1 und Metsulfuron im Frühjahr 1987 im frühen Nachauflauf mit 8 g AS ha?1 angewandt. Von jeder Versuchsfläche wurden über einen Zeitraum von 1 Jahr periodisch Bodenproben entnommen. Die Versuche wurden an 3 Standorten bis 1989 durchgeführt. Nach der Weizenernte 1989 wurden mit Biotests die Rückstände von Chlorsulfuron und Metsulfuron in den Böden mit jährlicher (über 3 Jahre) sowie mit einmaliger Behandlung bestimmt. Die Chlorsulfuron-Rückstände nahmen an 3 Standorten bei beiden Aufwandmengen nach 12 Monaten auf 0,3 g AS ha?1 ab. Am 4. Standort, wo während der Untersuchung nur 250 mm Niederschlag gefallen waren, wurden nach 15 Monaten noch signifikante Rückstände gefunden. Der Abbau von Chlorsulfuron folgte an allen Standorten bei einer Aufwandmenge von 30 g AS ha?1 einer Kinetik erster Ordnung mit Halbwertszeiten von 149, 70, 59 und 51 Tagen. Die Mobilität von Chlorsulfuron war an 3 Standorten hoch, während es in dem schluffigen Tonboden des 4. Standortes trotz hohen Niederschlags in den oberen 30 cm verblieb. Das im Frühjahr ausgebrachte Metsulfuron wurde nur in der 1. Bodenprobenserie gefunden. An einem Standort lagen in allen beprobten Bodenschichten Rückstände vor, doch sonst waren sie auf die oberste Bodenschicht beschränkt. Bei wiederholter Anwendung konnte keine Herbizidakkumulation im Boden festgestellt werden.  相似文献   
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