Glutamate dehydrogenase and glutamine synthetase activity in the digestive tract in lambs in relation to age]     

K Holovská  V Lenártová  J Koppel  I Havassy  P Javorsky  E Rybosová 《Veterinární medicína》1991,36(10):625-632

Developmental dynamics was investigated in the activity of glutamate dehydrogenase (GDH, E.C. 1.4.1.2.-4) and glutamine synthetase (GS, E.C. 6.3.1.2) in different parts of the digestive tract of lambs, in dependence on the age from 10 to 90 days; the goal of these investigations was to elucidate in greater detail the role of the above enzymes in nitrogen metabolism. The activity of GDH, and of the coenzymes NADH and NADPH, was followed in the digesta because simple organisms (bacteria, fungi, plants) have two glutamate dehydrogenases: they differ from each other by coenzyme specificity, unlike GDH from animal sources which can utilize both NADH coenzyme and NADPH coenzyme (Fahien et al., 1965; Frieden, 1964). The following activities of GDH and GS were found out in trials with lambs at the age of 10, 20, 30, 40 and 90 days, as to the different parts of digestive tract: in the tissues of rumen, omasum, reticulum, spleen, duodenum, jejunum, ileum, int. caecum and colon the activity of GDH (NADH) varied from 0.031 to 0.305 nkat/mg dry matter, in the digesta from 0 to 2.92 nkat/mg dry matter. An investigation of GDH (NADH, NADPH) dynamics in the digesta of lambs showed the relatively high activity of GDH (NADH) in the digesta of colon at the age of 10 days and that of GDH (NADPH) in the digesta of int. caecum. The activity of GDH (NADH) was also found to be high in the digesta of int. caecum at the age of 20 days. In that period the activity of GDH (NADH, NADPH) in the digesta of rumen, omasum and reticulum was zero.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Digestibility and nitrogen retention of diets containing different levels of fibre in local (Mong Cai), F1 (Mong Cai x Yorkshire) and exotic (Landrace x Yorkshire) growing pigs in Vietnam     

Len NT  Lindberg JE  Ogle B 《Journal of animal physiology and animal nutrition》2007,91(7-8):297-303

Total tract digestibility and nitrogen retention of three diets containing different levels of fibre [200, 260 and 320 g/kg neutral detergent fibre (NDF) in dry matter] were determined in three breeds of growing pig at an initial age of approximately 3.5 months. The breeds were local (Mong Cai, MC), F1 crossbred (MC x Yorkshire) and exotic (Landrace x Yorkshire, LY), allocated at random within breed (block) to double 3 x 3 Latin squares. The main fibrous ingredients of the experimental diets were rice bran, cassava residue meal and non-dehulled groundnut cake meal. Digestibility of organic matter (OM), crude protein (CP), NDF, crude fibre, gross energy (GE) and ether extract (EE) decreased as the level of dietary NDF increased (p < 0.001). The r(2) values for the relationship between NDF level and digestibility of OM, CP, GE and EE were 83%, 83%, 80% and 82% respectively. On average, an increase in NDF content of 1% unit resulted in a decrease in OM, CP, GE and EE digestibility of 0.67%, 0.75%, 0.50% and 0.42% units respectively. Digestibility of energy and nutrients was the highest for MC and the lowest for LY (p < 0.01), with intermediate values for F1. There was a negative effect of NDF level on nitrogen (N) retained as a proportion of intake (p < 0.05). Nitrogen retention and utilization were significantly higher (p < 0.001) for LY than for MC and F1 pigs.  相似文献   

鸭梨感染黑星病后生理病变的研究   总被引：2，自引：0，他引：2  

范燕萍  冷怀琼 《华南农业大学学报》1996,17(1):37-40

通过对鸭梨叶片接种梨黑星菌分生孢子后，分期测定其叶片的细胞渗透性，光合作用，总酚含量，多酚氧化酶活性以及过氧化物酶同工酶，结果表明：鸭梨叶片感染黑星病后，细胞渗透性增加，光合作用降低，总酚含量增加；多酚氧化活性增强，过氧化物酶同工酶也发生变化，在接种后有新酶带产生和原酶带活性增强。  相似文献   

新疆石河子市及周边地区奶牛子宫内膜炎的发病规律调查     

李正国  罗瑞卿  宋华容  关辉  张飞  吴磊  覃杰  谷新利 《中国奶牛》2009,(6)

AK2007年11月到2008年10月,对新疆石河子市及周边地区的14个奶牛养殖场进行了子宫内膜炎发病规律调查,结果发现,该地区成年母牛的子宫内膜炎的平均发病率为22.39%,奶牛子宫内膜炎的发病和产犊季节、奶牛年龄、胎衣不下、分娩方式、胎次、生产环境等因素有关.  相似文献