首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   356篇
  免费   17篇
  国内免费   1篇
林业   8篇
农学   7篇
基础科学   12篇
  66篇
综合类   42篇
农作物   28篇
水产渔业   9篇
畜牧兽医   181篇
园艺   3篇
植物保护   18篇
  2022年   2篇
  2021年   5篇
  2020年   5篇
  2019年   7篇
  2018年   3篇
  2016年   9篇
  2015年   7篇
  2014年   8篇
  2013年   16篇
  2012年   17篇
  2011年   28篇
  2010年   21篇
  2009年   21篇
  2008年   20篇
  2007年   25篇
  2006年   31篇
  2005年   20篇
  2004年   16篇
  2003年   13篇
  2002年   13篇
  2001年   4篇
  2000年   3篇
  1999年   3篇
  1997年   2篇
  1996年   2篇
  1994年   2篇
  1993年   2篇
  1990年   2篇
  1989年   3篇
  1987年   2篇
  1986年   3篇
  1985年   3篇
  1984年   2篇
  1981年   2篇
  1980年   3篇
  1979年   2篇
  1976年   2篇
  1974年   2篇
  1971年   5篇
  1970年   2篇
  1968年   7篇
  1967年   4篇
  1966年   2篇
  1963年   3篇
  1962年   1篇
  1960年   2篇
  1959年   1篇
  1958年   1篇
  1957年   1篇
  1950年   1篇
排序方式: 共有374条查询结果,搜索用时 15 毫秒
101.
102.
103.
Early detection of Johne’s disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is essential to reduce transmission; consequently, new diagnostic techniques and approaches to detect MAP or markers of early MAP infection are being explored. The objective was to identify biomarkers associated with MAP infection at 6 and 9 months after oral inoculation. Therefore, gene expression analysis was done using whole blood cells obtained from MAP-infected calves. All MAP-inoculated calves had a cell-mediated immune response (IFN-γ) to Johnin PPD specific antigens, and 60% had an antibody response to MAP antigens. Gene expression analysis at 6 months after inoculation revealed downregulation of chemoattractants, namely neutrophil beta-defensin-9 like peptide (BNBD9-Like), S100 calcium binding protein A9 (s100A9) and G protein coupled receptor 77 (GPR77) or C5a anaphylatoxin chemotactic receptor (C5a2). Furthermore, BOLA/MHC-1 intracellular antigen presentation gene was downregulated 9 months after inoculation. In parallel, qPCR experiments to evaluate the robustness of some differentially expressed genes revealed consistent downregulation of BOLA/MHC-I, BNBD9-Like and upregulation of CD46 at 3, 6, 9, 12, and 15 months after inoculation. In conclusion, measuring the expression of these genes has potential for implementation in a diagnostic tool for the early detection of MAP infection.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-014-0096-5) contains supplementary material, which is available to authorized users.  相似文献   
104.
105.
This paper has two purposes: To report the findings of a study of ethnic differences in cognition in a rural West Sumatran area; and to demonstrate the importance of ethnicity—in at least some contexts—for tailoring agricultural research to farmers' needs. A cognitive mapping technique, called a Galileo, was used to measure people's views of soil and its relation to people among three Indonesian ethnic groups living in the same area. Findings from participant observation and from collaborative agricultural fieldwork with farmers of all three ethnic groups are used to evaluate and interpret the Galileo results.Carol J. Pierce Colfer is currently Associate Professor at Sultan Qaboos University in the Sultanate of Oman. She is an anthropologist who served first as the farming systems researcher, and later as Team Leader of the Tropsoils-Indonesia project in Sitiung, with the University of Hawaii.Barbara Newton is Professor of Psychology at the University of Hawaii, West Oahu. She has had extensive experience with Galileo research and analysis.  相似文献   
106.
The large-scale spatial dynamics and population structure of marine top predators are poorly known. We present electronic tag and photographic identification data showing a complex suite of behavioral patterns in white sharks. These include coastal return migrations and the fastest known transoceanic return migration among swimming fauna, which provide direct evidence of a link between widely separated populations in South Africa and Australia. Transoceanic return migration involved a return to the original capture location, dives to depths of 980 meters, and the tolerance of water temperatures as low as 3.4 degrees C. These findings contradict previous ideas that female white sharks do not make transoceanic migrations, and they suggest natal homing behavior.  相似文献   
107.
The full potential of organ transplantation has not yet been realized because of the hazards associated with the long-term use of immunosuppressive drugs. Modern research into mechanisms of immune tolerance offers the promise of reprogramming the immune system, so as to harness the body's natural tolerance mechanisms in the service of graft acceptance. This would allow the minimization of immunosuppressive treatment and offers the prospect of eventually weaning transplant recipients off their drugs.  相似文献   
108.
109.
Lead immobilization was evaluated on soils spiked with increasingconcentrations of Pb (as Pb-acetate) using the following soilamendments: bentonite, zeolite, cyclonic ash, compost, lime,steelshot, and hydroxyapatite. The immobilization efficacy of theamendments was evaluated according to the following criteria:Ca(NO3)2-extractable Pb as an indicator of Pbphytoavailability, morphological and enzymatic parameters of beanplants (Phaseolus vulgaris) as indicator of phytotoxicity, and Pb concentration in edible tissue of lettuce (Lactuca sativa). The lowest reductions in Ca(NO3)2-extractablesoil Pb occurred when bentonite and steelshot were applied. Phytotoxicity from application of steelshot was confounded by toxic amounts of Fe and Mn released from the by-product which killed the lettuce seedlings. Addition of zeolite induced poorplant growth independent of Pb concentration due to its adverseeffect on soil structure. Substantial reductions in Ca(NO3)2-extractable Pb were observed when cyclonic ash, lime, compost and hydroxyapatite were applied. In general,these amendments reduced Pb phytotoxicity concomitant with reduced Pb concentration in lettuce tissue. Cyclonic ash, limeand compost further improved plant growth and reduced oxidativestress at low soil Pb concentrations due to soil pH increase mitigating Al or Mn toxicity.  相似文献   
110.
Model for Simulation of Particle Flow on a Centrifugal Fertiliser Spreader   总被引:2,自引:2,他引:2  
An analysis of the motion of a particle on a rotating flat disc is conducted and a basic equation of motion derived for a single particle. The analysis is directly applied to the flow of particles on a centrifugal fertiliser spreader. Equations of motion are derived for flat disc/radial vanes type of spreaders and further generalised for the more common conical disc/pitched vanes type. The derived equations of motion form part of the basis of a simulation model for centrifugal (or spinning disc) fertiliser spreaders with the equations of motion through the air completing the model. In the simulation, the collective flow of particles is treated as a superposition of single-particle trajectories with different initial conditions. The spreader model is applicable for the development of spreader control systems for site-specific fertiliser application as well as for the design and optimisation of spreaders.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号