ULTRASOUND-GUIDED TISSUE-CORE BIOPSY OF LIVER, SPLEEN AND KIDNEY IN NORMAL DOGS     

Lieve M.J.H. de  Rycke  DVM  Henri J.J. van  Bree  DVM  PhD  Paul J.M.  Simoens  DVM  PhD 《Veterinary radiology & ultrasound》1999,40(3):294-299

Six normal dogs were subjected to ultrasound-guided biopsy of the liver, spleen and kidney to examine the accuracy of the technique (i.e. the presence of targeted tissue) and the histologic quality of the biopsies. Five consecutive tissue-core biopsies of each organ were taken on one or more occasions. The accuracy of the technique was 77% for hepatic, 90% for splenic, 53.5% for left kidney and 40% for right kidney biopsies. The histologic quality of the liver and kidney samples was sufficient, although for some samples the diagnostic value was limited by their size and in renal samples either cortical or medullary tissue was sometimes lacking. In contrast, the quality of the splenic sections was not good. The effect of reused and resterilized needles on the quality of the specimens was evaluated by histologic inspection of the samples and by the amount of biopsies lacking tissue. All tissue samples, including those taken with reused or resterilized needles had sharp-cut edges. Twenty-two of the total number of 120 biopsies (18%) contained no tissue. Absence of tissue in the samples was observed in biopsies taken with all needle types. The animals were observed for possible complications of the repeated needle biopsy. Apart from one case of hematuria, no complications were encountered.  相似文献   

Physiological and genetic control of tuber formation   总被引：7，自引：0，他引：7  

Paul C. Strunik  Dick Vreugdenhil  Herman J. van Eck  Christian W. Bachem  Richard G. F. Visser 《Potato Research》1999,42(2):313-331

Summary Tuber formation is a plalstic and complex, but well-orchestrated sequences of morphological. physiological and biochemical events. The physiological control mechanisms of this sequence of events may involve many hormones, but certainly gibberellic acids play a dominant role: they affecft most steps and are influenced by inducing or non-inducing conditions in a manner consistent with effects of these conditions on tuber induction. The genetic control is also complex. Numerous cDNA fragments have been isolated which are specifically expressed during tuberization. They can be used in anti-sense orientation in transgenic plants to thest their possible role in tuberization. In addition these cDNA fragments are used as genetic marker loci in QTL mapping studies and serve as candidate genes to exlain phenotypic variation. Profiles of the importance of QTLs over time may be combined with expression profiles of candidate genes. Such novel approaches offer unique opportunities for synergism between physiology, molecular biology and genetics.  相似文献   

Collaborative study of clopidol in chicken tissues and eggs, using gas-liquid chromatography     

E L Bjerke  J L Herman 《Journal of the Association of Official Analytical Chemists》1974,57(4):914-918

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Effects of Density Reduction and Supplemental Feeding on Stunted Crayfish Procambarus clarkii Populations in Earthen Ponds1     

Herman H. Jarboe  Robert P. Romaire 《Journal of the World Aquaculture Society》1995,26(1):29-37

Density reduction, supplemental feeding, and a combination of two treatments were evaluated as means to alleviate stunting of red swamp crayfish Procambarus clarkii in earthen ponds. Mean total yield of crayfish in low density control ponds averaged 1,218 kg/ha and was 1.5 to 2 times higher (P≤ 0.05) than either the high density control or treatment ponds. Mean individual harvest weight of crayfish averaged 15.1 g and did not differ among treatments. Mean total yield of crayfish in ponds receiving supplemental feed was 32% higher than non-fed ponds (P≤ 0.05). Mean total yield and size of crayfish at harvest from reduced and non-reduced treatments did not differ. Density reduction and supplemental feeding implemented in early April did not increase individual crayfish weight to the commercially desirable size of 20 g.  相似文献   

Simulation of alternatives for the Dutch Johne's disease certification-and-monitoring program     

Weber MF  Groenendaal H  van Roermund HJ  Nielen M 《Preventive veterinary medicine》2004,62(1):1-17

To identify optimal method(s) for certification and subsequent monitoring of Mycobacterium avium subsp. paratuberculosis (Map)-unsuspected herds, certification-and-monitoring schemes were studied using a stochastic simulation model ("JohneSSim"). JohneSSim simulated the within-herd transmission and economic aspects of Map in closed Dutch dairy herds. The model was validated with field observations on Map-unsuspected herds. The current Dutch certification-and-monitoring schemes were compared with 11 alternative schemes in which individual and pooled fecal culture, ELISA, Johnin-intradermal test and gamma-IFN ELISA were used, varying the test frequency, tested age group and number of tested animals. On reaching the 'Map-free' status with the standard certification scheme, 11% of the simulated herds were not truly Map-free. Therefore, the designation 'Map-free' should be changed into, for instance, 'low-risk Map'. In the most-attractive alternative certification scheme, the 'Map-free' status was reached after four herd examinations (at 2-year intervals) consisting of serial testing of all cattle > or = 2 years of age with a pooled fecal culture and individual fecal culture of positive pools. This scheme resulted in lower total and annual discounted costs and a lower animal-level prevalence at reaching the 'Map-free' status compared to the standard scheme, assuming that there was no new introduction of the infection. Schemes to monitor the 'Map-free' status were compared, assuming that this status was reached with the standard certification scheme. In comparison to the standard monitoring scheme, none of the alternative monitoring schemes resulted in both a lower animal-level prevalence of undetected pre-existing Map infections in closed herds, and lower median annual discounted costs. Results of the model were very sensitive to the assumed sensitivity of the fecal culture test and to management measures that prevent within-herd transmission of Map. If these preventive measures were taken, the probability of undetected Map infections in closed 'Map-free' herds was decreased substantially.  相似文献   

Confirmation organizational decision of private practice certification and recognition     

Geertsen H 《Tijdschrift voor diergeneeskunde》2004,129(19):647

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Myopathy and hepatic lipidosis in weaned lambs due to vitamin E deficiency          下载免费PDF全文

Menzies P  Langs L  Boermans H  Martin J  McNally J 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(3):244-247

A sheep flock experienced losses in weaned lambs from myopathy and hepatic lipidosis. Investigation revealed painful ambulation, illthrift, and unexpected death in lambs with normal selenium levels, deficient vitamin E levels, and elevated muscle and liver enzyme levels. Vitamin E deficiency should be considered when investigating myopathy and illthrift in lambs.  相似文献   

Arctic paleo-oceanography in late cenozoic time     

Herman Y 《Science (New York, N.Y.)》1970,169(3944):474-477

Sediment cores from the Arctic Ocean yield significant faunal and lithologic evidence of alternating cold and milder periods for the last 6 million years. Although high-latitude continental glaciation commenced prior to 6 million years ago, the Arctic Ocean remained free of permanent pack ice up to approximately 0.7 million years ago, after which successive ice-covered and ice-free conditions existed.  相似文献   

Association of maximum voluntary dietary intake of freeze-dried garlic with Heinz body anemia in horses     

Pearson W  Boermans HJ  Bettger WJ  McBride BW  Lindinger MI 《American journal of veterinary research》2005,66(3):457-465

OBJECTIVE: To characterize hematologic and clinical consequences of chronic dietary consumption of freeze-dried garlic at maximum voluntary intake in horses. ANIMALS: 4 healthy sex- and age-matched horses. PROCEDURE: An initial garlic dose (0.05 g/kg, twice daily) was fed to 2 horses in a molasses carrier as part of their normal ration and was gradually increased to maximum voluntary intake (0.25 g/kg, twice daily) over 41 days. Dietary supplementation then continued for a total of 71 days. Two control horses were fed molasses with no garlic with their ration. Blood samples were collected weekly and analyzed for hematologic and biochemical changes, including the presence of Heinz bodies. Recovery of affected blood values was followed for 5 weeks after termination of dietary supplementation with garlic. RESULTS: At a daily dose of > 0.2 g/kg, horses fed garlic developed hematologic and biochemical indications of Heinz body anemia, as characterized by increases in Heinz body score (HBS), mean corpuscular volume (MCV), mean corpuscular hemoglobin, platelet count, and serum unconjugated and total bilirubin concentrations and decreases in RBC count, blood hemoglobin concentration, mean corpuscular hemoglobin concentration, and serum haptoglobin concentration. Recovery from anemia was largely complete within 5 weeks after termination of dietary supplementation with garlic. Heinz body score and MCV remained high at the end of the 5-week recovery period. CONCLUSIONS AND CLINICAL RELEVANCE: Horses will voluntarily consume sufficient quantities of garlic to cause Heinz body anemia. The potential for garlic toxicosis exists when horses are chronically fed garlic. Further study is required to determine the safe dietary dose of garlic in horses.  相似文献   

Bacterial eggshell contamination in conventional cages, furnished cages and aviary housing systems for laying hens   总被引：2，自引：2，他引：0  

De Reu K  Grijspeerdt K  Heyndrickx M  Zoons J  De Baere K  Uyttendaele M  Debevere J  Herman L 《British poultry science》2005,46(2):149-155

The influence of housing system on the initial bacterial contamination of the eggshell was studied. Two long-term experiments were performed. Bacterial eggshell contamination, as expressed by total count of aerobic and Gram-negative bacteria, was periodically analysed for eggs from a conventional cage, a furnished cage with nest boxes containing artificial turf or grids as nest-floor material and an aviary housing system. Results were log-transformed prior to statistical analyses. For both experiments no systematic differences were found between the conventional cage and furnished cage. The type of nest-floor material in the nest boxes of the furnished cages also did not systematically influence the bacterial contamination. A possible seasonal influence on contamination with a decrease in the winter period (up to > 0.5 log cfu/eggshell) of total count of aerobic and Gram-negative bacteria was observed in the first experiment. The contamination with total aerobic flora was higher (more than 1.0 log) on eggs from the aviary housing system compared to the conventional and the furnished cage systems. For Gram-negative bacteria this was not the case. During the entire period of both experiments, independent of housing system, shell contamination was not influenced by age of hens or period since placing the birds in the houses. For the total count of aerobic bacteria a restricted positive correlation (r2 = 0.66) was found between the concentration of total bacteria in the air of the poultry houses and initial shell contamination.  相似文献