David and Goliath in a dog's blood          下载免费PDF全文

Ricardo Marcos  Marta Santos  Liliana Leite‐Martins  Miguel França  Augusto de Matos 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(4):475-476

  相似文献   

Evolution of bovine brucellosis in Colombia over a 7-year period (2006–2012)     

Liliana Cárdenas  Oscar Melo  Jordi Casal 《Tropical animal health and production》2018,50(1):19-27

  相似文献   

Pharmacokinetics and pharmacodynamic effects in koi carp (Cyprinus carpio) following immersion in propofol     

Ayako Oda  Kristen M. Messenger  Liliana Carbajal  Lysa P. Posner  Brett R. Gardner  Scott H. Hammer  Anthony J. Cerreta  Gregory A. Lewbart  Kate M. Bailey 《Veterinary anaesthesia and analgesia》2018,45(4):529-538

Objective

To test the hypothesis that plasma propofol concentration (PPC) is associated with anesthetic effect in koi carp administered propofol by immersion.

Study design

Prospective study.

Animals

Twenty mature koi carp (mean ± standard deviation, 409.4 ± 83.7 g).

Methods

Fish were immersed in propofol (5 mg L^–1). Physiological variables and induction and recovery times were recorded. In phase I, blood was sampled for PPC immediately following induction and at recovery. In phase II, following induction, fish were maintained with propofol (4 mg L^–1) via a recirculating system for 20 minutes. Following established induction, blood was sampled at 1, 10 and 20 minutes. In phase III (n = 19), fish were anesthetized as in phase II with blood sampled nine times in a sparse sampling strategy. Simultaneously, a pharmacodynamics rubric was used to evaluate anesthetic depth. PPC was determined using high performance liquid chromatography with fluorescence detection. Following evaluation of normality, data were analyzed using paired t test or Spearman correlation test (significance was set at p < 0.05).

Results

In phase I, mean PPCs at induction (20.12 μg mL^–1) and recovery (11.62 μg mL^–1) were different (p < 0.001). In phase II, only mean PPCs at induction (17.92 μg mL^–1) and 10 minutes (21.50 μg mL^–1) were different (p = 0.013). In phase III, a correlation between PPCs and the pharmacodynamic rubric scores was found (p < 0.001, r = –0.93). There was no correlation between PPCs and recovery time (p = 0.057, r = 0.433). A two-compartment open model was chosen for the pharmacokinetic model. Absorption rate constant, elimination rate constant and intercompartmental rate constant were 0.48, 0.006 and 0.02 minute^–1, respectively.

Conclusions and clinical relevance

Measurable PPCs were achieved in koi carp anesthetized with propofol by immersion. Anesthetic depth of fish was negatively correlated with PPCs, but recovery time was not.  相似文献   

The use of biochemical,sensorial and chromaticity attributes as indicators of postmortem changes in commercial‐size,cultured red porgy Pagrus pagrus,stored on ice     

George Vardanis  Liliana Sfichi‐Duke  Lluis Tort  Pascal Divanach  Kiriakos Kotzabasis  Michail Pavlidis 《Aquaculture Research》2011,42(3):341-350

The freshness of red porgy slaughtered in ice slurry and stored in melting ice was evaluated instrumentally, biochemically and sensorialy. Additionally, postmortem skin colour changes were monitored, in an attempt to demonstrate the use of chromaticity parameters as a reliable and convenient approach to quality assessment. Dielectric properties showed a statistically significant decrease (P<0.05) from 12±0 on day 0 to 7.85±0.31 on day 7; there was also a significant decrease in muscle polyamines with more than two amino groups (spermidine and spermine) and an increase in the di‐amine putrescine. The sensorial score was significantly decreased from 30±0 to 4.33±0.21 on day 7. Minimal lightness (L^*) and hue (H°ab) at the dorsal skin area were observed on days 1 and 3 following harvesting. Finally, there was a marked decrease in the entire colour index (ECI – a combination of skin hue and chroma) of both the dorsal and the ventral area in day 3 onwards to day 7, as well as a statistically significant correlation between all the estimated freshness indices and ECI. Based on all these, we inferred that the total polyamines (or putrescine to spermidine and spermine ratio) and ECI could be reliable estimators of freshness, at least under the experimental conditions applied.  相似文献   

Ailinella mirabilis gen. n., sp. n. (eucestoda: pseudophyllidea) from Galaxias maculatus (Pisces: Galaxiidae) in the Andean-Patagonian region of Argentina     

de Pertierra AA  Semenas LG 《Folia parasitologica》2006,53(4):276-286

Ailinella gen. n. (Pseudophyllidea: Triaenophoridae) is proposed to accommodate Ailinella mirabilis sp. n. from Galaxias maculatus (Jenyns, 1842), a freshwater fish inhabiting the Andean lakes in Argentinean Patagonia. Ailinella belongs to the Triaenophoridae because it has a marginal genital pore, a follicular vitelline gland, and a ventral uterine pore. The new genus can be distinguished from other triaenophorids by the following combination of characters: a small body size, a low number of proglottides, which are longer than wide, a truncated pyramidal to globular scolex, a rectangular apical disc, presence of the neck, lack of internal longitudinal musculature separating the cortex from the medulla, testes distributed in one central field surrounding the ovary laterally and posteriorly, the vagina predominantly anterior to the cirrus sac, vitelline follicles circum-medullary, the genital pores post-equatorial, a saccate uterus, and operculate eggs. Blade-like spiniform microtriches were present on all tegument surfaces, and tumuli on all surfaces of the scolex and the anterior surface of the neck. Microtriches were characterized according to their size and density, and tumuli according to their size, inter-tumulus distance and density. Ailinella mirabilis is the first cestode described from G. maculatus and the second triaenophorid species recorded from a South American freshwater fish.  相似文献   

Moment of addition of LH to the culture medium improves in vitro survival and development of secondary goat pre-antral follicles     

Silva CM  Castro SV  Faustino LR  Rodrigues GQ  Brito IR  Saraiva MV  Rossetto R  Silva TF  Campello CC  Figueiredo JR 《Reproduction in domestic animals》2011,46(4):579-584

The present study investigated the effects of time of addition of luteinizing hormone (LH) to culture medium on the in vitro development of caprine pre-antral follicles. Pre-antral follicles (≥ 150 μm) were isolated from fragments of the goat ovarian cortex and individually cultured for 18 days in the absence (control) or presence of 100 ng/ml LH, added on days 0, 6 or 12 of culture. Follicular development was assessed based on antral cavity formation, increased follicular diameter as well as follicular and fully grown oocyte (>110 μm) viability. The results showed that after 18 days of culture, the percentage of surviving follicles in the control treatment was significantly lower when compared to other treatments (p < 0.05). There were no significant differences in antrum formation, follicular diameter and oocyte viability. The addition of LH at D6 of culture significantly increased the rates of oocytes ≥ 110 μm and the resumption of meiosis (p < 0.05). In contrast, when LH was added at the onset of culture, only germinal vesicle oocytes were obtained. In conclusion, the moment of addition of LH to the culture medium affects the performance of in vitro culture of caprine pre-antral follicles. The addition of LH to the medium from day 6 of culture onward improved the rates of follicular survival, as well as the ability of oocytes to resume meiosis. However, prolonged exposure to LH (addition at the onset of culture onward) showed detrimental effects for the meiotic resumption.  相似文献   

Cytokine-enhanced vaccine and interferon-β plus suicide gene as combined therapy for spontaneous canine sarcomas     

Finocchiaro LM  Villaverde MS  Gil-Cardeza ML  Riveros MD  Glikin GC 《Research in veterinary science》2011,91(2):230-234

Eleven soft tissue- and five osteosarcoma canine patients were subjected to: (i) periodic subcutaneous injection of irradiated xenogeneic cells secreting hGM-CSF and hIL-2 mixed with allogeneic or autologous tumor homogenates; and (ii) injections of cIFN-β and HSVtk-carrying lipoplexes and ganciclovir, marginal (after surgery) and/or intratumoral (in the case of partial tumor resection, local relapse or small surface tumors). This treatment alone (4 patients) or as surgery adjuvant (12 patients), was safe and well tolerated. In those patients presenting local disease (6/11), the suicide gene plus cIFN-β treatment induced local antitumor activity evidenced by the objective responses (3 complete, 2 partial) and stable diseases (2). In addition, the treatment prevented or delayed local relapse, regional metastases (lymph nodes developed only in 3/16) and distant metastases (0/16), suggesting a strong systemic antitumor immunity. The most encouraging result was the long survival times of 10 patients (>1 year, with good quality of life).  相似文献   

Food security and biodiversity: can we have both? An agroecological analysis     

Michael?Jahi?ChappellEmail author  Liliana?A.?LaValle 《Agriculture and Human Values》2011,28(1):3-26

We present an extensive literature review exploring the relationships between food insecurity and rapid biodiversity loss, and the competing methods proposed to address each of these serious problems. Given a large and growing human population, the persistence of widespread malnutrition, and the direct and significant threats the expanding agricultural system poses to biodiversity, the goals of providing universal food security and protecting biodiversity seem incompatible. Examining the literature shows that the current agricultural system already provides sufficient food on a worldwide basis, but in doing so methodically undermines the capacity of agroecosystems to preserve biodiversity. However, the available evidence emphasizes the interdependence of biodiversity and agriculture, and the important role each plays in the maintenance of the other. Thus, our review supports the claim that the solutions to the problems of widespread food insecurity and biodiversity loss need not be mutually exclusive, and that it may be possible to address both using appropriate alternative agricultural practices.  相似文献   

Accuracy of the composite somatic cell count to detect intra-mammary infection in dairy cows using latent class analysis     

Claudina Vissio  Silvana Andrea Dieser  Héctor Luis Agnelli  Liliana Mónica Odierno  Alejandro José Larriestra 《Preventive veterinary medicine》2014

The somatic cell count (SCC) is considered an important indicator of intra-mammary infection (IMI). The purpose of this study was to determine the accuracy of both SCC and culture to detect IMI and their conditional dependence by means of latent class methods. This study involved 175 dairy cows from 2 herds with different udder infection prevalences. Quarter and composite milk samples were collected for SCC and bacteriological culture. Latent-class models using Bayesian methods were used to estimate test sensitivity (Se) and specificity (Sp) and population prevalence. The models ran involved only major mastitis pathogens and composite SCC (CSCC). Five thresholds between 100,000 and 300,000 cells/mL were evaluated and the receiver operating characteristics (ROC) curve analysis was performed. Fifty-five percent of the cows had CSCC ≥200,000 cells/mL and 95.4% of the cows had at least one infected quarter either with minor or major pathogens. Considering a threshold of 150,000 cells/mL, the estimated Se and Sp for the CSCC were, 0.80 (95% CrI 0.71–0.88) and 0.57 (95% CrI 0.44–0.71), respectively. The estimated culture Se and Sp were 0.83 (95% CrI 0.73–0.93) and 0.89 (95% CrI 0.74–0.98), respectively. There was no evidence of dependence between CSCC and culture. The area under curve for CSCC was 0.72. To the best of our knowledge, this is the first report of the CSCC accuracy to detect IMI for major pathogens considering the effect of culture misclassification. The estimates provided here could help to examine the performance of sampling schemes based on CSCC to manage udder health.  相似文献   

Nitrogen Mineralization from Sugarcane Vinasse     

Alinne da Silva  Raffaella Rossetto  Juliana Bombecini  Michel Piemonte  Takashi Muraoka 《Journal of plant nutrition》2014,37(8):1227-1236

Vinasse is a liquid residue applied as a fertilizer in sugarcane crops. However, in areas far away from sugarcane mills the cost of the distribution can be very high due, the large volume of water. So, one solution is to concentrate the vinasse by the evaporation process to reduce transport costs. Considering that the nitrogen mineralization kinetics is not known in concentrated vinasse, the objective of this study was to evaluate the net and potential nitrogen mineralization in soil that received vinasse concentrated and not concentrated. The treatments with concentrated vinasse provided the highest values of net mineralization and total nitrogen, which presented significant correlation each other, the same way as the potential mineralization values had a positive correlation with the doses and the amounts of total nitrogen. The total nitrogen values can be used as an index of nitrogen availability in soils that received concentrated vinasse.  相似文献