Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats     

Juliana Paula Martins Alves  Csar Carneiro Linhares Fernandes  Rafael Rossetto  Caroline Pessoa da Silva  Iolly Tbata Oliveira Marques Galvo  Marcelo Bertolini  Davide Rondina 《Reproduction in domestic animals》2019,54(9):1206-1216

The aim of this study was to verify the effect of the energy source for a short‐term diet supplementation on follicular dynamics, ovarian response and oocyte recovery in goats. Thirty Anglo Nubian crossbred does received a diet for 4 weeks to satisfy the nutritional requirements of breeding for adult non‐dairy goats. Seven days prior to oocyte recovery (OR), a group of does (n = 10) was supplemented with ground full‐fat linseed in the diet (Diet A), whereas a second group of does (n = 10) received crude glycerine in the diet (Diet B). The total mixed ration (TMR) diet was maintained as the Control Diet (n = 10). All animals were oestrous‐synchronized by the use of a progesterone insert for 12 days prior to OR. Follicles were stimulated by using pFSH (five 40‐mg/ml doses) during the supplementation time. At OR, follicles were counted and recovered oocytes were classified as viable or degenerated. Follicular dynamics was monitored by ultrasonography, and plasma glucose, cholesterol and triglyceride levels were measured during supplementation. Glucose was higher in Diet B and cholesterol in Diet A. Diet B had a lower proportion of small (<3 mm) and large follicles (≥3 mm; p = 0.01). The follicular growth rate was higher in Diet A (p < 0.01), with follicles emerging in the 5th day of supplementation. No differences were observed for follicles counted and oocytes recovered. Thus, the type of energy source supplemented for a short term was capable to alter the follicular dynamics, without affecting the proportion of morphologically viable oocytes upon recovery.  相似文献   

Phytotoxic and nematicide evaluation of Croton ehrenbergii (Euphorbiaceae)     

María Yolanda Rios  Ismael Len‐Rivera  Ramiro Ríos‐Gomez  Liliana C Crdova‐Albores  Angelica Berenice Aguilar‐Guadarrama 《Pest management science》2019,75(8):2158-2165

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Structural remodelling of the heart valves extracellular matrix during embryo development     

Luis Felipe Falla Zuñiga  Yhoiss Smiht Muñoz Cerón  Liliana Salazar 《Anatomia, histologia, embryologia》2021,50(1):206-211

Alterations in heart valve development represent more than 20% of congenital cardiovascular malformations. Most of the functional properties of heart valves depend on extracellular matrix. Despite its relevance, little is known about fibrillar components on developing stages. Our objective is to define histological changes on valves fibrillar components in late embryonic development of Mus musculus. We found type III collagen as the predominant fibre type in the ECM in prenatal stages followed by a switch to a type I predominance for postnatal ages. The change in fibrillar components is necessary to support the normal mechanical function of adult heart valves.  相似文献   

The nature and dynamics of bacterial genomes     

Ochman H  Davalos LM 《Science (New York, N.Y.)》2006,311(5768):1730-1733

Though generally small and gene rich, bacterial genomes are constantly subjected to both mutational and population-level processes that operate to increase amounts of functionless DNA. As a result, the coding potential of bacterial genomes can be substantially lower than originally predicted. Whereas only a single pseudogene was included in the original annotation of the bacterium Escherichia coli, we estimate that this genome harbors hundreds of inactivated and otherwise functionless genes. Such regions will never yield a detectable phenotype, but their identification is vital to efforts to elucidate the biological role of all the proteins within the cell.  相似文献   

Interaction between ascorbic acid and follicle-stimulating hormone maintains follicular viability after long-term in vitro culture of caprine preantral follicles     

R. Rossetto  I.B. Lima-Verde  M.H.T. Matos  M.V.A. Saraiva  F.S. Martins  L.R. Faustino  V.R. Araújo  C.M.G. Silva  K.P.O. Name  S.N. Báo SN  C.C. Campello  J.R. Figueiredo  H. Blume 《Domestic animal endocrinology》2009

This study evaluates the effects of ascorbic acid and its interaction with follicle-stimulating hormone (FSH) on the morphology, activation, and in vitro growth of caprine preantral follicles. Ovarian fragments were cultured for 1, 7, or 14 d in minimum essential medium (MEM) containing ascorbic acid (50 or 100 μg/mL), FSH (50 ng/mL), or both of these substances. Ovarian tissue that was either fresh (control) or cultured for 1, 7, or 14 d was processed for histological and ultrastructural evaluation. The results showed that after 14 d of culture, medium supplemented with 50 μg/mL of ascorbic acid alone or combined with FSH showed higher rates of follicular survival compared with MEM. After 7 d of culture, FSH, ascorbic acid at 50 μg/mL with or without FSH, and ascorbic acid at 100 μg/mL increased the percentage of follicular activation compared to fresh control. In addition, FSH alone significantly increased the percentage of growing follicles after 14 d. The combination of 50 μg/mL of ascorbic acid and FSH promoted a significant increase in oocyte and follicular diameter after 7 d of culture. Ultrastructural and fluorescent analysis confirmed the integrity of follicles cultured with 50 μg/mL of ascorbic acid and FSH after 14 d. In conclusion, the combination of 50 μg/mL of ascorbic acid and FSH maintained follicular integrity and promoted follicular activation and growth after long-term in vitro culture of caprine preantral follicles.  相似文献   

Properties of an aged phenanthrene-contaminated soil and its response to bioremediation processes   总被引：1，自引：0，他引：1  

Scelza  Rosalia  Rao  Maria A.  Gianfreda  Liliana 《Journal of Soils and Sediments》2010,10(3):545-555

Purpose  

Persistent organic compounds exhibit declining extractability and bioavailability to microorganisms and other soil organisms with increasing contact time or ageing. Among the possible mechanisms are the association of organic compounds with natural organic matter. Ageing can reduce the negative effects that an organic pollutant may have on the biological, biochemical properties and phytotoxicity of the contaminated soil. The aim of the present work was to evaluate, under laboratory conditions, the response of an agricultural soil contaminated with phenanthrene (Phe) and subjected to 2-year ageing and the effects and potential remediation capabilities of compost and an effective Phe-degrading bacterial culture.  相似文献   

Characterization of aflatoxigenic and non-aflatoxigenic strains of <Emphasis Type="Italic">Aspergillus</Emphasis> section <Emphasis Type="Italic">Flavi</Emphasis> isolated from corn grains of different geographic origins in Brazil     

Liliana de Oliveira Rocha  Gabriela Martins Reis  Raquel Braghini  Estela Kobashigawa  Jansen de Araújo  Benedito Corrêa 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(3):353-366

Aflatoxins can cause great economic losses and serious risks to humans and animals health. The largest aflatoxin producers belong to Aspergillus section Flavi and can occur naturally in food commodities. Studies showed that molecular tools as well as the type of sclerotia produced by the strains could be helpful for identification of Aspergillus species and could be correlated with levels of toxin production. The purpose of this work was to characterize the genetic diversity using AFLP technique, the type of sclerotia and the ability of aflatoxin production by isolated strains from corn of different origins in Brazil, and to verify whether qPCR based on aflR and aflP genes is appropriate for estimating the level of aflatoxin production. All the 75 strains were classified as A. flavus and the AFLP technique showed a wide intraspecific variability within them. Regarding sclerotia production, 34% were classified as S and 66% as L type. Among the aflatoxin-producers, 52.8% produced aflatoxin B₁, while 47.2% aflatoxins B₁ and B₂. Statistical analysis showed no correlation between sclerotia production and aflatoxigenicty, and no correlation between the phylogenetic clusters and aflatoxin production. Concerning the relative expression of aflR and aflP, Pearson’s correlation test demonstrated low positive correlation between the expression of the aflR and aflP genes and the production of AFB₁ and AFB₂, but showed high positive correlation between aflR and aflP expression. In contrast to the other reference strains, A. oryzae ATCC 7282 showed no amplification of aflR and aflP. The results highlight the need for detection of reliable and reproducible markers with a high positive correlation with aflatoxin production.  相似文献   

The effects of obesity and fatty acids on the feline immune system     

Jaso-Friedmann L  Leary JH  Praveen K  Waldron M  Hoenig M 《Veterinary immunology and immunopathology》2008,122(1-2):146-152

Obesity is a rising problem in cats. It is a risk factor for several diseases and has been linked to impaired immunity. The goal of this study was to determine the effect of body composition and effects of diet on immune function in cats. Twenty-eight short-term obese and 12 lean cats with equal gender distribution were evenly and randomly divided into two groups which were either fed a diet containing saturated (SFA) or long-chain n-3 polyunsaturated fatty acids (3-PUFA) for a period of 6 months prior to testing. Blood was collected by venipuncture from the jugular vein. Blood samples were analyzed in a double blind fashion. A complete blood count was performed and lymphocyte distribution was examined by flow cytometric analysis with specific fluorescein-conjugated subset markers. Immune function was measured as follows: the proliferative activity of different cellular fractions was tested with polyclonal mitogens such as lipopolysaccharide (LPS), phytohaemagglutinin (PHA), phorbol 12-myristate 13-acetate (PMA), Ca ionophore, and concanavalin A. Innate immune functions assessed were phagocytosis and natural killer cell (NK) cytotoxicity. A similar immune innate and adaptive immune response was elicited regardless of diet or body condition. However, there was no correlation between body condition, diet, and any of the quantitative and qualitative functional responses of the immune system. We conclude that short-term obesity and the fatty acid composition of the diet do not alter immune responses in cats.  相似文献   

In vitro polyploidization of Mecardonia tenella, a native plant from South America     

Alejandro S. Escandón  Liliana. M. AldereteJuan C. Hagiwara 《Scientia Horticulturae》2007

Mecardonia tenella is an herbaceous plant widely distributed in the temperate region of South America. Both plant architecture and flower size are characteristics that can be improved to become a viable new ornamental plant. Chromosome doubling by the use of agents such as colchicine is an available methodology to this end. Nodal segments from in vitro grown plants of M. tenella were submerged in the following doses of colchicine in 1% (v/v) dimethyl-sulfoxide (DMSO) solution (%, v/v): 0.0, 0.001 and 0.01 (24 and 48 h). The DNA content of the regenerated plants was measured by flow cytometer. A total of 68 tetraploid plants were detected out of 126 colchicine treated plants. The flowers and leaves of the tetraploid plants were bigger compared to those from the wild diploid type (control). Under field conditions, the selected tetraploid plants showed a more compact shape than the control plants.  相似文献   

Phenolic Acids Profiles and Cellular Antioxidant Activity in Tortillas Produced from Mexican Maize Landrace Processed by Nixtamalization and Lime Extrusion Cooking     

Nallely Gaxiola-Cuevas  Saraid Mora-Rochín  Edith Oliva Cuevas-Rodriguez  Liliana León-López  Cuauhtémoc Reyes-Moreno  Alvaro Montoya-Rodríguez  Jorge Milán-Carrillo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):314-320

Phenolic acids profiles, chemical antioxidant activities (ABTS and ORAC), as well as cellular antioxidant activity (CAA) of tortilla of Mexican native maize landraces elaborated from nixtamalization and lime cooking extrusion processes were studied. Both cooking procedures decreased total phenolics, chemicals antioxidant activity when compared to raw grains. Extruded tortillas retained 79.6–83.5%, 74.1–77.6% and 79.8–80.5% of total phenolics, ABTS and ORAC values, respectively, compared to 47.8–49.8%, 41.3–42.3% and 43.7–44.4% assayed in traditional tortillas, respectively. Approximately 72.5–88.2% of ferulic acid in raw grains and their tortillas were in the bound form. Regarding of the CAA initially found in raw grains, the retained percentage for traditional and extruded tortillas ranged from 47.4 to 48.7% and 72.8 to 77.5%, respectively. These results suggest that Mexican maize landrace used in this study could be considered for the elaboration of nixtamalized and extruded food products with nutraceutical potential.  相似文献