Systemic neosporosis in a dog treated for immune‐mediated thrombocytopenia and hemolytic anemia          下载免费PDF全文

Angie Magaña  Félix Sánchez  Karina Villa  Liliana Rivera  Elizabeth Morales 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2015,44(4):592-596

A 4‐year‐old male Toy Poodle was presented to the Small Animal Veterinary Hospital of the Faculty of Veterinary Medicine of the Autonomous University of Mexico (FMVZ, UNAM) because of depression, lethargy, and hemorrhages involving several areas of the skin and around the eyes. Hematology data and a bone marrow analysis suggested hemolytic anemia and immune‐mediated thrombocytopenia. The dog was treated with prednisone, and after one month the hematology variables improved. However, the dog's clinical condition inexplicably worsened and it was euthanized. On necropsy, there were no relevant findings. However, in histology, multifocal lymphoplasmacytic and histiocytic meningoencephalitis and necrosis, and a protozoan cyst in the cerebellum were identified. In addition, moderate multifocal lymphoplasmacytic and necrotizing pancreatitis, hepatitis, myocarditis, and diffuse lymphoplasmacytic enteritis were observed. Immunohistochemistry of the cerebellum, liver, pancreas, and intestine with a specific antibody against Neospora caninum confirmed the diagnosis of systemic neosporosis. The systemic neosporosis in this dog was most likely caused by reactivation of latent parasites due to prednisone administration during the one month of treatment. It should be kept in mind that in dogs being treated with immunosuppressants for immune‐mediated conditions, opportunistic parasites, such as Toxoplasma gondii and N caninum, can be reactivated from a latent state, as it probably happened in the present case.  相似文献   

Selection of watermelon genotypes for resistance to bacterial fruit blotch     

Francisco C. Q. Carvalho  Liliana A. Santos  Rita C. S. Dias  Rosa L. R. Mariano  Elineide B. Souza 《Euphytica》2013,190(2):169-180

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High rates of serum selenium deficiency among HIV- and HCV-infected and uninfected drug users in Buenos Aires, Argentina     

Sheehan HB  Benetucci J  Muzzio E  Redini L  Naveira J  Segura M  Weissenbacher M  Tang AM 《Public health nutrition》2012,15(3):538-545

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Epsilon Aminocaproic Acid for the Prevention of Delayed Postoperative Bleeding in Retired Racing Greyhounds Undergoing Gonadectomy     

Liliana M. Marín DVM  MSc  M Cristina Iazbik DVM  Sara Zaldivar‐Lopez DVM  MSc  Julien Guillaumin DVM  Diplomate ACVECC  Mary A. McLoughlin DVM  MSc  Diplomate ACVS  C Guillermo Couto DVM  Diplomate ACVIM 《Veterinary surgery : VS》2012,41(5):594-603

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Effect of cutting age and substrate temperature on rooting of <Emphasis Type="Italic">Taxus globosa</Emphasis>     

Liliana Muñoz-Gutiérrez  J. Jesús Vargas-Hernández  Javier López-Upton  Marcos Soto-Hernández 《New Forests》2009,38(2):187-196

Taxus globosa (Mexican yew) is a conifer endemic to México and Central America. It produces a substance known as taxol, which is useful in treatment of ovarian cancer. Because seed production for this dioecious tree is limited, and seed germination is extremely difficult, the use of cuttings could facilitate propagation of this species. With the intention of massively propagating individuals selected for taxol content, two trials were established in which the effect of substrate temperature (average temperatures 18 and 23°C), age (i.e., young vs. old shoots) and management of cuttings, as well as clone variation in rooting capacity, were evaluated. Low temperature favored rooting (53 vs. 34% on average for the two trials); younger shoots rooted three times (61 vs. 23%) more than mature ones, while basal wounding did not affect rooting capacity. A wide variation was found in rooting capacity of clones (8–76%), which could be associated with genetic or physiological differences among donor trees.  相似文献   

Domestication and saponins contents in a gradient of management intensity of agaves: <Emphasis Type="Italic">Agave cupreata</Emphasis>, <Emphasis Type="Italic">A. inaequidens</Emphasis> and <Emphasis Type="Italic">A. hookeri</Emphasis> in central Mexico     

Carmen Julia Figueredo Urbina  Alejandro Casas  Yesenia Martínez-Díaz  Liliana Santos-Zea  Janet A. Gutiérrez-Uribe 《Genetic Resources and Crop Evolution》2018,65(4):1133-1146

Saponins occur in numerous plants, including agaves, determining benefic and harmful properties to humans; their presence may favor using plants as soap and other products, but also they may cause caustic effects producing contact dermatitis. In domestication, favorable and unfavorable properties of saponins may cause an increase or decrease of their content, respectively. This study quantified and identified saponins among wild and managed populations of three agave species: A. cupreata Trel. et Berger, A. inaequidens Koch with wild and cultivated populations used for mescal production, and A. hookeri Jacobi, existing exclusively cultivated, used for production of the fermented beverage pulque. We studied 272 plants from 19 populations, quantifying contents of crude saponins through spectrometry. In 12 populations, the saponins types were identified by High Performance Liquid Chromatography–Mass-Spectrography-Time-of-Flight HPLC-MS-TOF. The highest crude saponins content was recorded in A. hookeri (26.09 mg/g), followed by A. cupreata (19.85 and 15.17 mg/g in wild and cultivated populations, respectively). For A. inaequidens, we recorded 14.21, 12.95, and 10.48 mg/g in wild, silvicultural managed and cultivated populations, respectively. We identified 18 saponins types, A. inaequidens showing all of them. A hecogenin glycoside (HG1) is found in high amounts in A. hookeri but in low quantities in A. inaequidens and A. cupreata. A. inaequidens had the greatest diversity of saponins. The contents of crude saponins in A. inaequidens and A. cupreata decrease with management intensity, but contrarily to what we expected, it was the highest in A. hookeri. We hypothesize that such high amount could be due to some saponins, probably HG1, may be precursors of sugars.  相似文献   

Preliminary study of a teaching model for ultrasound-guided peripheral nerve blockade and effects on the learning curve in veterinary anesthesia residents     

Luciano C.B.A. da Silva  Fábio P. Sellera  Ronaldo G. Gargano  Thais C. Rossetto  Giancarlo B. Gomes  Fabiano T. Miyahira  Fábio Futema  Silvia R.G. Cortopassi 《Veterinary anaesthesia and analgesia》2017,44(3):684-687

Objective

To evaluate the use of an experimental colloid model for teaching veterinary anesthesia residents ultrasound-guided technique for nerve blockade.

Study design

Prospective, blinded and randomized.

Methods

Colloid models were constructed for practice in ultrasound-guided needle location. Nine veterinary anesthesia residents with no prior experience of ultrasound-guided technique for nerve blocks were randomly divided into three groups. Each group received theoretical orientation. Two groups were assigned to practical training using the experimental model: group 1 (G1) received 2 hours of training and group 2 (G2) received 1 hour of training prior to testing with specific tasks. Group 3 (G3) received no practical training. During testing, the time required for task completion (e.g., display of structures and positioning a needle) and the number of failures were recorded.

Results

The average times to completion of the tasks and the number of technical failures were: G1, 47 seconds and 1 failure; G2, 68 seconds and 2 failures; G3, 187 seconds and 7 failures.

Conclusions and clinical relevance

In residents with no prior experience of ultrasound-guided needle placement, using an experimental colloid model and a longer training period was associated with increased accuracy and decreased time to task completion. Based on the results of this study, training with an experimental model can be recommended to improve the speed and accuracy of needle manipulation using ultrasound in clinicians with no prior experience of ultrasound-guided technique.  相似文献   

Variant-specific and diminishing immune responses towards the highly variable MSP2(P44) outer membrane protein of Anaplasma phagocytophilum during persistent infection in lambs     

Erik G. Granquist  Snorre Stuen  Liliana Crosby  Anna M. Lundgren  A. Rick. Alleman  Anthony F. Barbet 《Veterinary immunology and immunopathology》2010,133(2-4):117-124

Anaplasma phagocytophilum is the causative agent of tick-borne fever in small ruminants and has been identified as the zoonotic agent of human granulocytic anaplasmosis. The Norwegian strains of the rickettsia are naturally persistent in lambs and represent a suitable experimental system for analyzing the mechanisms of persistence. Variation of the outer membrane protein MSP2(P44) by recombination of variable pseudogene segments into an expression site is believed to play a key role in persistence of the organism. The goal of the present study was to analyze the dynamics of the immune response towards A. phagocytophilum and MSP2(P44) during persistent infection of lambs. Responses to the hypervariable region of MSP2(P44) were detected shortly after appearance of the respective variants in cyclic rickettsemic peaks, consistent with a process of antigenic variation. In addition, there was a diminishing antibody response to MSP2(P44) and to other A. phagocytophilum antigens overall with time of infection, that was not associated with clearance of the infection.  相似文献   

Prevalence of dog erythrocyte antigens in retired racing Greyhounds     

Iazbik MC  O'Donnell M  Marin L  Zaldivar S  Hudson D  Couto CG 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2010,39(4):433-435

Background: Blood groups in dogs are designated as dog erythrocyte antigen (DEA) 1.1, 1.2, 3, 4, 5, 7, and Dal. There is limited information about the frequency of different antigens in Greyhound dogs, despite their frequent use as blood donors. Objectives: The aims of this study were to determine the frequencies of DEA 1.1, 1.2, 3, 4, 5, and 7 in Greyhounds, to compare the frequencies with those of non‐Greyhound dogs, and to evaluate the presence of naturally occurring anti‐DEA antibodies. Methods: Blood was collected from 206 Greyhound and 66 non‐Greyhound dogs being screened as potential blood donors. Blood‐typing was performed at Animal Blood Resources International by tube agglutination utilizing polyclonal anti‐DEA antibodies. Results: Of the Greyhound dogs, 27/206 (13.1%) were positive for DEA 1.1, and this frequency was significantly lower (P<.0001) than for non‐Greyhound dogs of which 40/66 (60.6%) were DEA 1.1‐positive. The frequency of positivity for both DEA 1.1 and 1.2 was also lower in Greyhounds (P<.0001). There were no significant differences between Greyhounds and non‐Greyhounds for DEA 1.2, 3, 4, 5, or 7. All 137 dogs (113 Greyhounds and 24 non‐Greyhounds) that were evaluated for naturally occurring anti‐DEA antibodies in serum were negative. A higher percentage of Greyhound dogs (57.3%, 118/206) were considered “universal donors” (negative for all DEAs except DEA 4) compared with non‐Greyhound dogs (28%, 13/46). Conclusion: The frequency of positivity for DEA 1.1 in our population of Greyhounds was significantly lower than previously reported for dogs. Furthermore, a large majority of Greyhounds met the criteria for universal donors.  相似文献   

Fast and simple method for the simultaneous evaluation of the capacity and efficiency of food antioxidants in trapping peroxyl radicals in an intestinal model system     

Rossetto M  Vanzani P  De Marco V  Zennaro L  Scarpa M  Rigo A 《Journal of agricultural and food chemistry》2008,56(10):3486-3492

A simple oxygraphic method, for which the theoretical and experimental bases have been recently revised, has been successfully applied to evaluate the peroxyl radical chain-breaking characteristics of some typical food antioxidants in micelle systems, among which is a system that reproduces conditions present in the upper part of the digestive tract, where the absorption and digestion of lipids occur. This method permits one to obtain from a single experimental run the peroxyl radical trapping capacity (PRTC, that is, the number of moles of peroxyl radicals trapped by a given amount of food), the peroxyl radical trapping efficiency (PRTE, that is, the reciprocal of the amount of food that reduces to half the steady-state concentration of peroxyl radicals), and the half-life of the antioxidant ( t(1/2)) when only a small fraction of peroxyl radicals reacts with the antioxidants present in foods. Examples of application of the method to various types of foodstuffs have been reported, assessing the general validity of the method in the simple and fast evaluation of the above-reported fundamental antioxidant characteristics of foods.  相似文献