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This study was performed to evaluate the efficacy of acupuncture on induced chronic arthritis of the dog by thermography. Complete Freund's adjuvant was injected into the left knee joint of 8 dogs to induce arthritis. Acupuncture was applied to BL-40, GB-33, GB-34, and LIV-8 once a week for 4 consecutive weeks, from 3 weeks after induction of chronic arthritis, in treatment group. At 3 weeks of acupuncture treatment, skin temperature difference (DeltaT) of treatment group returned to normal range (< 0.3 degrees C), while DeltaT remained high in non-treatment group. Infrared thermography (IRT) is useful to evaluate the treatment of acupuncture for induced canine chronic arthritis. Therefore, it is considered that clinical application of IRT in arthritis treatment would be also valuable.  相似文献   
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To assess whether the antigenic properties of H5 hemagglutinin (HA) change over time due to antigenic drift, we produced a panel of monoclonal antibodies (mAbs) against the HA of the index H5N1 human influenza A virus, A/Hong Kong/156/97. By immunizing mice with a plasmid expressing this HA and boosting the initial immunization with cell lysates transfected with the plasmid, a total of six hybridomas producing HA-specific mAbs were established: four to the HA1 subunit with hemadsorption-inhibiting activity and two to the HA2 subunit. None of the mAbs to HA1 could bind to the HA of a recent human isolate, A/Hong Kong/213/2003, indicating that there are substantial antigenic differences between the H5N1 human influenza virus isolated in 1997 and that isolated in 2003.  相似文献   
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Most studies of oocyte apoptosis have been performed in vitro and have employed the method of artificial induction of apoptosis by an anti-cancer agent. However, the process of oocyte death in vivo has not been clearly identified. To investigate the death process in unfertilized oocytes in vivo, we examined the cytochemical change of oocytes collected by oviduct flushing at various intervals after hCG injection. At each collection time, the collected oocytes were phenotypically classified under the microscope into four groups: single-cell oocytes (non-activated and without a nucleus and cytokinesis), activated oocytes (single-, 2- or 4-cell with a nucleus), fragmented oocytes, and dead oocytes. The number of single oocytes decreased and dead oocytes increased with the lapse of time, but the number of activated oocytes or fragmented oocytes did not. Also, most of the dead oocytes observed were single cell. At each time point, single oocytes were stained with anti-tubulin antibody to examine their spindle status. At 24 h after hCG injection, all ovulated oocytes had a normal bipolar spindle, while at 64 h all single-cell oocytes had no spindle. From these observations, we concluded that most oocyte deaths in vivo occur in the single oocyte stage, not in activated or fragmented oocytes.  相似文献   
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The medical records of 23 dogs that underwent medial canthoplasty for treatment of epiphora were reviewed. The most prevalent breed encountered was the shih tzu. Other affected breeds included the Pekingese, Maltese, toy poodle, and pug. All dogs had epiphora associated with medial canthal trichiasis and/or entropion. Other ocular abnormalities included conjunctivitis, keratitis, pigmentary keratitis, corneal ulceration, globe prolapse, and nasal fold trichiasis. After medial canthoplasty, the epiphora resolved in all dogs.  相似文献   
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A 15-year-old female maltese was referred to us because of a 3-month history of ataxia, circling, and acute blindness. A mass was noted in the brainstem on brain magnetic resonance images. A cerebellar herniation was also detected on T1-weighted sagittal images. The lateral, third and fourth ventricles and central canal of the cervical spinal cord were enlarged. Based on diagnostic imaging findings, cervical syringomyelia secondary to a brainstem tumor was suspected. The clinical signs were controlled well by lomustine and the dog survived for 8 months after the initial diagnosis. The mass was diagnosed as a meningioma based on histopathological findings. This report describes the clinical findings and imaging characteristics of an acquired syringomyelia resulting from a brainstem meningioma.  相似文献   
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A 6-year-old ShihTzu presented with tonic-clonic cluster seizure. T2-weighted magnetic resonance (MR) images showed bilateral diffuse hyperintense lesions at the cerebral cortex with enlarged sulci. Computed tomography revealed a portosystemic shunt (PSS) and azygos continuation. Based on the clinical signs, blood examinations and diagnostic images, the dog was diagnosed with hepatic encephalopathy secondary to PSS. The neurologic signs were gradually improved after medical therapy for hyperammonemia. This is the first report of hyperintensity of the cerebral cortex on T2-weighted MR images associated with acute hepatic encephalopathy in a dog.  相似文献   
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