Transfer of Bovine Blastocysts Derived from Short-term In Vitro Culture of Low Quality Morulae Produced In Vivo     

RH Alvarez  M Meneghel  AC Martinez  RML Pires  EA Schammass 《Reproduction in domestic animals》2008,43(3):257-260

The aim of this study was to evaluate if blastocysts arising from in vitro culture of Grade 3 bovine morulae produced in vivo can promote acceptable pregnancy rates when transferred into recipients. Embryos of different stages and qualities were recovered from superovulated Bos taurus and B. indicus donors. Grade 3 morulae were cultured in either Holding Plus® or TCM‐199 (supplemented with 10% bovine fetal serum) media for 24 h at 38.5°C. After this culture period, the resulting blastocysts were morphologically classified (Grades 1, 2 and 3) and transferred into recipients previously synchronized with the donors. Non‐cultured Grades 1 and 3 morulae were used as control. Pregnancy diagnosis was carried out 60 days after embryo transfer and the data were analysed by logistic regression, considering variables, such as embryo quality (Grade), donor breed, culture medium, donor‐recipient synchrony and seasonality. Embryo quality was the only variable, showing significant effect on the pregnancy rate. Pregnancy rates for non‐cultured Grade 1 and 3 morulae, and blastocysts arising from cultured Grade 3 morulae were 58.1% (n = 31), 17.1% (n = 35) and 51.1% (n = 47), respectively (p < 0.05). There were no statistical differences between non‐cultured Grade 1 morulae and cultured blastocysts. Pregnancy rates for Grades 1 (65.0%) and 2 (60.0%) were higher than Grade 3 (29.4%) cultured blastocysts (p < 0.05). It was concluded that short‐term in vitro culture is a very convenient method of identifying morphologically low quality morulae with higher chances of continuing development after the transfer into recipients.  相似文献   

Unsuitability of indigenous South American Rutaceae as potential hosts of Diaphorina citri     

Patrícia A de C Felisberto  Eduardo A Girardi  Leandro Pea  Guilherme Felisberto  George AC Beattie  Silvio A Lopes 《Pest management science》2019,75(7):1911-1920

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Measurement of cell wall penetration in wood of water-based chemicals using SEM/EDS and STEM/EDS technique   总被引：2，自引：1，他引：1  

L. Wallström  K. A. H. Lindberg 《Wood Science and Technology》1999,33(2):111-122

Summary The penetration of bulking chemicals (glycerol, PEG 200, PEG 1500 and pentaerythritol) into the cell wall of wood, Pinus sylvestris, has been studied. A number of different methods for determining the distribution of chemicals in the cell wall were used. Measurements of the increase in cell wall thickness showed that glycerol and PEG 200 resulted in greater cell wall bulking compared to PEG 1500 and pentaerythritol. Examination with SEM/EDS-linescan confirmed these results. However, the better resolution possible with the STEM/EDS-linescan revealed an inhomogenous distribution of the chemical in the cell wall. This is believed to be due to microcracks in the cell wall which are the result of the initial drying of the wood. This general damage to the cell wall could be the reason for the failure to find a stabilizing chemical and method. Received 10 February 1997  相似文献   

Influence of Physical Training and Pre-Slaughter Exercise on Fluid Retention,Colour and Adenosine Triphosphate of Pork Muscle     

Claes Rülcker  Paul Lindberg  Nils Lannek 《Acta veterinaria Scandinavica》1967,8(2):189

Wateriness and a pale colour seriously impair the quality of pork and have given rise to an extensive literature (cf. 1). No reports have appeared, however, on the effects of physical training of the pigs.  相似文献   

Diagnosis of bacterial kidney disease by detection of Renibacterium salmoninarum by real-time PCR     

Jansson E  Lindberg L  Säker E  Aspán A 《Journal of fish diseases》2008,31(10):755-763

Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), is a serious threat to salmon in aquaculture as well as to wild populations. We have developed a real-time polymerase chain reaction (PCR) for detection of Rs in kidney samples. The PCR is based on detection of unique parts of the 16S rRNA gene of Rs and DNA equivalent to 1-10 Rs genomes was detected per reaction. No cross-reactivity with other fish pathogenic or related bacteria could be demonstrated. Analysis of individual kidney samples collected from BKD classified populations identified 39.9% of the fish as positive by real-time PCR compared with 28.0% by polyclonal enzyme-linked immunosorbent assay (ELISA). The real-time PCR assay was found to be well suited for complementary use with ELISA for diagnosis of BKD, with the ability to detect clinical as well as covert Rs infections. The infection level determined by the polyclonal ELISA and by real-time PCR was significantly correlated.  相似文献   

Association between butyrate and short-chain fatty acid concentrations in gut contents and faeces in weaning piglets     

H.N. Lrke  M.S. Hedemann  K.E. Bach Knudsen  P. Laurinen  J.-E. Lindberg  C. Pedersen 《Livestock Science》2007,108(1-3):163-166

The concentration and composition of short-chain fatty acids (SCFA) in gastrointestinal and faecal samples were studied in weaning pigs fed different amounts and composition dietary fibre (DF). In Trial 1 a total of 50 castrated piglets were fed five experimental diets with varying contents of citrus pectin (soluble fibre) and barley hulls (insoluble fibre) and gastrointestinal contents were collected at euthanasia 9 days after weaning. In Trial 2, 120 pair-wise penned piglets were allocated to the same experimental diets as in Trial 1 (24 piglets per treatment), and fresh faecal samples were collected 5 and 32 days after weaning.

There was no difference in the concentrations of SCFA caused by dietary treatments, but across treatments there was a correlation between concentration of SCFA and the proportion of butyrate. The correlation was lowest in the caecum (r² = 0.22, P = 0.0007) and highest in the distal colon (r² = 0.45, P = 0.0001). In faeces there was no relationship 5 days after weaning (r² = 0.004. P = 0.64) but a correlation of r² = 0.25 (P = 0.0001) 32 days after weaning. The results suggest that adaptive changes and differences between piglets in fermentative capacity immediately after weaning are associated not only with a higher concentration of SCFA but also a changed fermentation profile.  相似文献   

Effect of cooking and fermentation of a pig diet on gut environment and digestibility in growing pigs   总被引：1，自引：0，他引：1  

Tran Thi Thu Hong  Jan Erik Lindberg   《Livestock Science》2007,109(1-3):135-137

The effect of pre-feeding treatment of a pig diet on gut environment and digestibility was studied in a double 3 × 3 Latin-square experiment using growing castrated PVTC cannulated male pigs. The diets were based on local feed resources and were fed raw (R), cooked (C) or naturally fermented (F). There were no differences (P > 0.05) in pH and butyric acid concentration of ileal digesta between diets. However, on diet F concentrations of acetic, lactic and propionic acid in ileal digesta were higher (P < 0.05) than on diets R and C. The relative proportions of individual organic acids in ileal digesta were not a reflection of the fermentation profile found in diet F. The ileal apparent digestibility of crude protein, crude fiber and NDF were higher (P < 0.05) on diet F than on diets R and C. The total tract apparent digestibility of crude protein was higher (P < 0.05) on diet F diet than on diets R and C, while there were no differences in total tract apparent digestibility between diets for any other dietary component. In conclusion, when compared with a pig diet in the raw form, fermentation influenced the gut environment and improved the digestibility of some dietary component, while cooking prior to feeding had no measurable effects.  相似文献   

Influence of phytase and xylanase supplementation of a wheat-based diet on ileal and total tract digestibility in growing pigs     

J.E. Lindberg  K. Lyberg  J. Sands 《Livestock Science》2007,109(1-3):268-270

The effect of phytase and xylanase supplementation of a wheat-based pig diet on the ileal and total tract apparent digestibility of dietary components and minerals were studied in eight growing pigs fitted with a PVTC cannula in a randomized block design experiment. The diets (A and B) were similar in major ingredient composition and in nutrient content. In diet A, part of the limestone was replaced with di-calcium phosphate to increase the content of available phosphorus (P). Diet B was fed without or with supplementation with phytase (500 FTU/kg; diet BP), xylanase (4000 XU/kg; diet BX) and phytase + xylanase (500 FTU and 4000 XU/kg; diet BPX). There were no differences (P > 0.05) between diets in the ileal or total tract digestibility of organic matter (OM), NDF and crude protein (CP). The ileal and total tract digestibility for P and Ca differed (P < 0.05) between diets, while there were no treatment effects for Zn. The ileal and total tract digestibility for P and Ca was higher (P < 0.05) on diets BP and BPX than on the other diets. In conclusion, phytase improved the utilization of dietary P and Ca in a wheat-based diet, while xylanase had no additional benefits in terms of OM and CP digestibility or mineral utilization. Phytase had no effect on the digestibility of OM, CP or NDF.  相似文献   

Onchocercosis of an intervertebral joint capsule causing cervical vertebral stenotic myelopathy in a horse.     

Gete Hestvik  Stina Ekman  Ronny Lindberg 《Journal of veterinary diagnostic investigation》2006,18(3):307-310

A novel case where onchocercosis was identified as a cause of cervical myelopathy in the horse is described. A 15-year-old Connemara mare was euthanized due to progressive locomotion disturbance. Postmortem examination revealed soft-tissue swelling in the intervertebral joint capsule of C6-7 with narrowing of the vertebral canal. On light microscopy, axonopathy was pronounced in the corresponding segment of the spinal cord. Fibrous tissue and eosinophilic granulomas were found in the joint capsule, together with parasites identified histologically as Onchocerca sp.  相似文献   

Letter: Blocked utilization of oxygen in pigs during exercise     

N. Lannek  P. Lindberg  L. Jonsson  G. Johansson 《Acta veterinaria Scandinavica》1973,14(3):492-494

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