Evaluation of Simple Sequence Repeat (SSR) Markers Established in Europe as a Method for the Identification of Potato Varieties Grown in Canada   总被引：1，自引：0，他引：1  

Marie-José Côté  Lisa Leduc  Alex Reid 《American Journal of Potato Research》2013,90(4):340-350

The correct identification of potato varieties is crucial to maintaining the quality level of seeds produced under the Canadian Seed Potato Certification Program. During inspection of in vitro potato plant propagation centres or seed potato production field lots, morphological characteristics may not be sufficient for the identification of plantlets or tubers and therefore molecular identification is sought by inspectors for variety confirmation. With international harmonization of testing methods in mind, we proposed to evaluate further the microsatellite (SSR) markers established successfully by Reid et al. (2009, Euphytica 182: 239–249, 2011), using the reference potato variety DNA collection at the Canadian Food Inspection Agency (CFIA) established and currently used for Amplified Fragment Length Polymorphism (AFLP) genotyping. The SSR markers developed by the European community laboratories were successfully validated by and established within the CFIA laboratory. Most genotypes generated for a set of 34 varieties were identical between the 2 laboratories with only 3 discrepancies due to the different interpretation of the presence/absence of the alleles. When used with potato reference DNAs of the CFIA collection, the method successfully differentiated 217 varieties apart but 10 groups, which are most likely clonal variants, were not discriminated. The SSR markers were successfully used to address 5 potato variety verification requests from CFIA inspectors during field inspections for seed potato certification. The markers successfully confirmed the presence of rogue varieties in 4 of these requests, therefore fulfilling the CFIA’s mandate towards stakeholders of the Canadian potato industry in preserving the quality of certified seeds.  相似文献   

Studies on inflammation in the sheep,using turpentine-induced pleurisy as a test system     

J.L. Vegad B.V.Sc. Assoc.I.V.R.I. Ph.D.  M.C. Lancaster B.Sc. M.R.C.V.S. Ph.D. 《New Zealand veterinary journal》2013,61(12):232-237

Extract

The interpretation of biochemical measurements usually requires knowledge of the normal as a reference point. It has been usual to take specimens from a group of individuals presumed to be healthy and to express as normal the range, or mean and standard deviation, found for those subjects.  相似文献   

Efficacy of Epidural Morphine Versus Intravenous Morphine for Post-Thoractotomy Pain in Dogs     

Sulli Popilskis  Dennis F. Kohn  Lisa Laurent  Peter Danilo 《Veterinary anaesthesia and analgesia》1993,20(1):21-25

We compared the effects of epidural and intravenous morphine on analgesic effectiveness, vital signs, cortisol and catecholamine concentrations in dogs. Twelve healthy mongrel dogs undergoing experimental thora-cotomy under non-opioid general anaesthesia were randomly assigned to two groups: group A (n=6) received 0.15 mg/kg morphine epidurally, and group B (n=6) received 0.15 mg/kg morphine IV. Postoperatively, animals were observed hourly for the first 10 hours, then at 18 and 24 hours, and heart rate, systolic and diastolic blood pressure, respiratory rate and pain score were measured. Serum cortisol and plasma catecholamines were measured at 0, 2, 4, 8 and 24 hours postoperatively. Epidural morphine was associated with a significant reduction in pain, cortisol levels, systolic blood pressure (all p<.01) and heart rate (p<.05). There was a direct correlation between pain score, cortisol and catecholamine concentrations in each group; both were lower in the group receiving epidural morphine. Our data indicate that epidural morphine is safe, effective and provides an alternative approach for treatment of post-thoracotomy pain.  相似文献   

A familial degenerative neuromuscular disease of Gelbvieh cattle.     

Peter G Moisan  David J Steffen  Michael W Sanderson  Jerome C Nietfeld  Melissa R Finley  Dale M Grotelueschen  Gordon A Andrews  Gayle Johnson  Lisa Williamson  Steven D Rushton  D Greg Hall  Barry G Harmon 《Journal of veterinary diagnostic investigation》2002,14(2):140-149

A degenerative skeletal muscle disease with vascular, neurologic, and renal lesions and a probable familial distribution was identified in 4-20-month-old purebred Gelbvieh cattle. Thirteen affected animals were confirmed from 6 separate beef herds, with a mortality rate of 100%. Clinical signs in affected animals consisted of ataxia, weakness, and terminal recumbency. Gross and histologic muscle lesions were indicative of nutritional myopathy of ruminants, with a lack of myocardial lesions in most cases and only rare myocardial changes in a few animals. Acute to chronic lesions in most large skeletal muscle groups consisted of degeneration, necrosis, regeneration, fibrosis, and atrophy. Fibrinoid necrosis of arterioles was a common feature in multiple tissues. Lesions in the spinal cord white matter and peripheral nerves consisted of degeneration of the dorsal columns and axons, respectively. Changes in the kidneys consisted of chronic interstitial nephritis with fibrosis, hyaline droplet change and tubular epithelial vacuolar change and were most severe in the older calves. Intracytoplasmic myoglobin and iron were demonstrated within the hyaline droplets in degenerate renal cortical tubular epithelial cells. Vitamin E levels were deficient in most (6/7) of the animals tested. Investigation of the pedigree of affected animals revealed a common ancestry for all but 1 of the animals whose parentage could be traced. This investigation suggests that a hereditary metabolic defect, possibly involving antioxidant metabolism, could be responsible for this condition. Renal disease, possibly secondary to myoglobinuria, may be unique to this bovine condition.  相似文献   

Colloid Osmotic Pressure of Parenteral Nutrition Components and Intravenous Fluids     

Daniel L. Chan DVM    Lisa M. Freeman DVM  PhD  DACVN    Elizabeth A. Rozanski DVM  DACVIM  DACVECC  John E. Rush DVM  MS  DACVIM  DACVECC 《Journal of Veterinary Emergency and Critical Care》2001,11(4):269-273

Objective: Parenteral nutrition is an important part of therapy for critically ill animals that cannot tolerate enteral feedings. It has been hypothesized that parenteral nutrition might also play a role in increasing colloid osmotic pressure (COP). The purpose of this study was to measure COP of various parenteral nutrition components and compare them to the COP of commonly used intravenous solutions.
Design: Membrane colloid osmometry was used to measure the COP of parenteral nutrition components (lipids, Abstractamino acids, dextrose solutions) and of synthetic colloids, crystalloids, and blood products.
Main Results: Parenteral nutrition components and all crystalloid solutions had COP measurements < 1 mm Hg. Great variation in COP was found in the different artificial colloids and blood products. The COP of the artificial colloids tested ranged from 32.7 ± 0.2 mm Hg for hetastarch to 61.7 ± 0.5 mm Hg for dextran 70.
Conclusions: The results of this in vitro study suggest that parenteral nutrition does not directly contribute to an increase in oncotic pressure. Further studies are needed to determine whether parenteral nutrition may indirectly influence COP in vivo. Knowing the COP of a fluid, along with its other properties, is useful in making appropriate therapeutic decisions.  相似文献   

猪链球菌是常见的共栖菌     

郑家林  梁启明  Sandy Amass  Lisa Tokach  Steve Henry  Enric Marco 《国外畜牧学(猪与禽)》2002,(1):47-47

猪链球菌感染常常很复杂 ,因为猪的扁桃体内和鼻腔中存在多种不同的血清型和不同的菌株。必须用与所见临床病例有关的特定菌株进行流行病学研究。用新的分子生物学技术来对同一血清型的不同菌株进行比较 ,必然很快就能使我们更充分地认识这一疾病  相似文献   

Platelet closure time in anesthetized Greyhounds with hemorrhagic shock treated with hydroxyethyl starch 130/0.4 or 0.9% sodium chloride infusions          下载免费PDF全文

Duana McBride BVSc  DACVECC  MVMedSc  FHEA  Anthea Raisis PhD  DVA  BVSc  MACVSc  M VetClinSt  Lisa Smart BVSc  DACVECC 《Journal of Veterinary Emergency and Critical Care》2016,26(4):509-515

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Landscape and local effects on occupancy and densities of an endangered wood-warbler in an urbanizing landscape     

Jennifer L. Reidy  Frank R. ThompsonIII  Courtney Amundson  Lisa O’Donnell 《Landscape Ecology》2016,31(2):365-382

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Development of stand structural stage indices to characterize forest condition in Upstate New York     

Lisa Goodell  Don Faber-Langendoen 《Forest Ecology and Management》2007

Stand development stages are often used to describe temperate forest dynamics, but defining them using stand history or age can be difficult, especially for extensive surveys. The purpose of this study was to define stand development stage indices for forest stands on state forest land in Upstate New York using stand structure alone. A total of 508 stands were sampled, including 442 in Upstate New York. Two structural stage indices were defined for each of the 442 stands. The first index, based on SILVAH, was calculated using weighted mean diameter of trees in different size classes. The second index, based on work from Frelich and Lorimer, was calculated using the ratio of basal area of large trees to mature trees and either (1) exposed crown area (ECA) of trees in different size classes, or (2) basal area of trees in different size classes. Both indices were tested on stands with known stand histories. The second index was preferred for its superior ability to distinguish old-growth stands and was used to characterize structural stages of major forest types in Upstate New York: hemlock–hardwood (9% of state forest land), red spruce-fir (16%), and northern hardwood (51%). Results showed that the vast majority of stands in Upstate New York are in the mid-developmental stages. Structural characteristics of old-growth stands from this study were found to be in agreement with other old-growth stands in the northeastern United States.  相似文献   

不同蠹害区的蠹害木清理试验     

刘宏屏  李浩然  陈鹏  李丽莎  杨金荣  王光明  查广林 《西部林业科学》2000,(2):38-42

在松纵坑切梢小蠹的综合防治中,蠹害木的清理是一重要环节。依据在轻、中、重受蠹害云南松林区进行的蠹害木清理试验结果,一般情况下,对轻度受害区即被害株率在10%以下,蠹害指数在5以下,清理蠹害木应控制在1.00%;对中度受害区,即被害株率在10%～50%,蠹害指数在5～15,清理蠹害木应控制在10.00%左右;重度受害区,即被害株率在50%以上,蠹害指数在15以上,清理蠹害木应控制在20.00%左右。在重度受害区,除清理蠹害木外,还需辅以其他措施,进行综合治理才能有效控制蠹害。  相似文献