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61.
Seventeen adult and cyclic Moxoto goats were synchronized using 60 mg MPA vaginal sponge for 11 days and 50 μg cloprostenol, 48 h before sponge removal, and superovulated with 120 mg pFSH i.m. in decreasing doses at 12 h intervals for three consecutive days. In seven goats, 0.2 IU/kg BW/day of long acting insulin was subcutaneously injected at same time as pFSH, and in the other five goats, the same dose of insulin was injected for three consecutive days starting 24 h after mating. Finally, five goats were supplemented with an oral dose of 80 ml/goat/day of propylene glycol continuously during the experiment. The animals were flushed at 7 days after mating and the embryos were classified based on International Embryo Transfer Society criteria. Blood samples were collected every 3 days for insulin assay. Administration of insulin raised the insulin levels of the goats (p < 0.05), whereas in the group treated with propylene glycol, insulin rate was different only between FSH treatment and after mating (p < 0.05). Similar rates of recovery for total (80.05 ± 9.78%) or transferable structures (61.03 ± 15.13%) were obtained. Treatment was not influenced (p > 0.05) by responsiveness to superovulation, which averaged 64%. By contrast, insulin treatments were shown to increase the number of embryos considered excellent with respect to goats supplemented with propylene glycol (p < 0.05). When insulin was given before mating, a strong relationship (r = 0. 90) (p < 0.05) between number of transferable embryo and ovulations was observed in the animals. In conclusion, superovulated goats treated with low doses of exogenous insulin resulted in an enhancement in embryo quality, which was related to changes in circulating insulin concentrations.  相似文献   
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The objectives were to evaluate the reproductive indices and survival analysis of pregnancy outcomes in multiparous repeat breeder Holstein cows (n = 557). The cows were synchronized to ovulate by Ovsynch, new controlled internal drug release device (N‐CIDRsynch), and once‐used CIDR device (U‐CIDRsynch). The pregnancy per AI at 28 days post‐insemination (P/AI 28) in the N‐CIDRsynch group (28.75%) was significantly (COR = 1.49; p = 0.011) greater than that reported in the Ovsynch (23.46%) and U‐CIDRsynch (21.73%) groups. Furthermore, the pregnancy per AI at day 75 post‐insemination (P/AI 75) in the N‐CIDRsynch group was significantly greater than the Ovysync group (COR = 1.35; p = 0.050). The repeat breeder cows received a N‐CIDR device had a significantly higher progesterone level on day 2 and day 4 of CIDR insertion (1.38 and 1.67 ng/ml, respectively) than those received a U‐CIDR device or the control group (p = 0.012 and 0.001, respectively). The Cox regression model recorded significant associations for synchronization protocols, THI at the TAI and season of calving with the hazard of P/AI 28 and P/AI 75 (p = 0.044 and 0.046; 0.001 and 0.005; 0.003 and 0.001, respectively). Multiparous repeat breeder cows (>3) had a lower hazard ratio (HR) of P/AI 28 than that reported in the reference (2nd parity) group (HR = 0.74, p = 0.050). The repeat breeder cows inseminated at 76–80 and >80 temperature‐humidity index (THI) had significantly lower HR of P/AI 28 than those inseminated at the baseline (<70) THI value (HR = 0.73 and 0.30, p = 0.036 and 0.001, respectively). The current results indicate that the use of N‐CIDR synch protocol may achieve satisfactory pregnancy outcomes in repeat breeder cows.  相似文献   
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从肿头综合征鸡分离出产Vero毒素E.coli   总被引:1,自引:1,他引:0  
本研究从哈尔滨郊区某鸡场肿头综合征( S H S)鸡分离出 3 株血清型为 O78 的埃希氏大肠杆菌( E.coli),从鞍山 S H S病料分离出 1 株血清型为 O8 的 E.coli。并证实这 4 株 E.coli 能够对 Vero 细胞产生毒性作用,与产 Vero 毒素的 E.coli一致。对哈尔滨市郊 S H S病鸡做病理组织学观察,主要变化为头部皮下组织,头盖骨气室及鼻粘膜下纤维素性化脓性炎症,水肿和大肠杆菌性肉芽肿,心、肝、肾等器官炎症变化并有大量异嗜性粒细胞和细菌团块。  相似文献   
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A prospective study was undertaken to reconcile radiographic cardiomegaly and normal echocardiography in obese cats and to test a radiographic technique for better distinguishing between pericardial fat and the heart. Ten obese, but otherwise normal cats and 10 non-obese normal cats were used. A body condition scoring system was used to objectively group obese and non-obese normal cats. Two-dimensional echocardiograms were made to verify that all cats had a normal heart. Thoracic radiographs then were made using standard and altered exposure techniques. The hearts were measured on these radiographs using the metric and a vertebral scale system. Obese cats consistently had excessive fat around the heart especially if they also had a large amount of falciform fat. Altering exposure technique by increasing mAs and decreasing kVp sometimes enhanced the radiographic contrast between fat and myocardium. Enhanced radiographic contrast accentuated the double silhouette identifying the true cardiac silhouette within the fat expanded silhouette. Pericardial fat usually was distinguished more readily in lateral than in VD radiographs. In two cats, pericardial fat had a characteristic prominent square corner to the right cranial margin of the cardiac silhouette in VD radiographs. Obesity caused increased width and depth of the thoracic cage. 2D echocardiograms revealed normal cardiac dimensions in both groups of cats and increased precordial distance in the obese group.  相似文献   
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• Identification of bacteriocin sources• Classification of bacteriocins• Antiviral pathways of bacteriocinsThe COVID-19 infections caused by SARS-CoV-2 have resulted in millions of people being infected and thousands of deaths globally since November 2019. To date, no unique therapeutic agent has been developed to slow the progression of this pandemic. Despite possessing antiviral traits the potential of bacteriocins to combat SARS-CoV-2 infection has not been fully investigated. This review summarizes the mechanisms by which bacteriocins can be manipulated and implemented as effective virus entry blockers with infection suppression potential properties to highly transmissible viruses through comprehensive immune modulations that are potentially effective against COVID-19. These antimicrobial peptides have been suggested as effective antiviral therapeutics and therapeutic supplements to prevent rapid virus transmission. This review also provides a new insight into the cellular and molecular alterations which have made SARS-CoV-2 self-modified with diversified infection patterns. In addition, the possible applications of antimicrobial peptides through both natural and induced mechanisms in infection prevention perspectives on changeable virulence cases are comprehensively analyzed. Specific attention is given to the antiviral mechanisms of the molecules along with their integrative use with synthetic biology and nanosensor technology for rapid detection. Novel bacteriocin based therapeutics with cutting-edge technologies might be potential substitutes for existing time-consuming and expensive approaches to fight this newly emerged global threat.  相似文献   
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The adoption strategies used in animal shelters can have a large impact on the total number of adoptions and donations that take place. Reducing adoption fees during peak kitten or puppy season is one way to reduce inventories and increase the number of open spaces to save more lives, but does not necessarily increase the financial well-being of the shelter if the per-animal costs exceed the revenues generated. We developed a stochastic model to simulate the expected costs, revenues, and net income of a hypothetical animal shelter for various alternative management strategies, based on US conditions. A total of 8 scenarios were developed and compared to the base-case scenario (BC).  相似文献   
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