冬季牛舍中三种吸附剂对NH3、CO2和H2S的吸附     

侯良忠  邵伟  郭守立  尹劲  亚青喜  余雄 《新疆农业科学》2015,52(12):2327

[目的]选用三种不同孔径分子筛吸附剂对冬季奶牛圈舍中CO2 、NH3和H2S吸附试验.[方法]用便携式气体检测仪检测排风扇口排出的CO2 、NH3和H2S浓度,悬挂吸附剂的为试验组,不悬挂吸附剂的为对照组.两者之差即为吸附剂XF-1、XF-2、XF-3的吸附浓度,根据实际测得的气温、气压、风速,利用理想气体状态方程推导出公式,将mg/kg换算为mg/m3.当试验组与对照组浓度无差异性时停止试验.[结果]1 kgXF-1吸附剂可吸附CO2 70.91 g、NH3 2.29 g、H2S1.57 g;1kg XF-2吸附剂可吸附CO2 75.49 g、NH3 2.65g、H2S 1.65 g;1kg XF-3吸附剂可吸附CO2 85.95 g、NH3 2.87 g、H2S 1.88 g.[结论]三种吸附剂对CO2 、NH3和H2S气体的吸附量与圈舍内相应气体浓度正相关性显著(P＜0.05),与温湿度负相关性不显著(P＞0.05).吸附剂的吸附能力与总孔体积、比表面积成正比,吸附剂XF-3对气体吸附能力最强,吸附剂XF-1最弱.三种吸附剂在1～3h吸附效果最好,此后缓慢下降,吸附剂XF-1悬挂30 h需要更换,吸附剂XF-2悬挂27h需要更换,吸附剂XF-3悬挂25h需要更换.  相似文献   

驴抗猪繁殖与呼吸综合征病毒IgG注射液的制备工艺及质量监控     

押宗保  李六金  李明涛  朱秋侠  马国际  田宏雄  李勇  刘学通 《动物医学进展》2004,25(6):127-129

用繁殖与呼吸障碍综合征病毒灭活抗原加佐剂接种关中驴 ,采用基础免疫和强化免疫程序 ,于接种后 9～ 1 1d采集其血浆 ,从中分离 ,提纯免疫球蛋白IgG。制备出抗PRRSV特异性的IgG注射液。对制备出的五批IgG注射液产品进行了血清中和试验 ,结果显示IgG抗体效价均达 1∶32 0以上 ,表明该法可以成功制备抗猪繁殖与呼吸障碍综合征病毒免疫球蛋白IgG注射液 ,并对其制备工艺及质量监控进行了多次试验 ,结果显示该生物制剂制造的工艺流程重复性好 ,IgG获得率较理想 ,质量稳定  相似文献   

用花粉管通道法导入Prd29A:DREB1A融合基因获得转基因小麦植株     

梁高峰  押辉远  谷运红  宋笑明  秦广雍 《核农学报》2008,22(3):256-259

采用花粉管通道法遗传转化技术,将Prd29 A:DREB1 A融合基因导入小麦品种温麦19。共处理温麦19的小花800朵,获得241粒种子,结实率为30.9%,通过PCR检测和GUS组织化学分析,证明外源的Prd29 A:DREB1 A融合基因已经整合到其中5个T0代植株的基因组中。  相似文献   

离子束诱变小麦高蛋白突变体的SSR分析     

陈秋芳  焦浈  押辉远  秦广雍 《核农学报》2008,22(6):745-749

利用SSR标记技术,对从离子束注入小麦的后代中选到的株高变矮、蛋白含量提高的突变体M6进行分析,其中有两对引物扩增出与对照有差异的DNA片段。将差异DNA片段回收、克隆、测序、序列对比,结果显示,与对照相比,突变体扩增的DNA片段主要表现为碱基的置换和缺失,其中有一个DNA片段序列与对照同源性较低,经BLASTn搜索,发现该变异序列与普通小麦(Triticum aestivum)BAC克隆的一段序列匹配率达到95%。同时发现该序列与圆锥小麦(Triticum turgidum)高分子量麦谷蛋白(Agenome HMW glutenin)位点中的一段序列匹配率也达到95%。  相似文献   

低能离子束注入甘草的刺激效应和诱变效应的RAPD分析   总被引：1，自引：0，他引：1  

张祥胜  押辉远 《河南农业科学》2007,(12):83-86

利用生物材料专用的低能离子束注入机,分别以低能(30 keV)Ar+0(对照),600×(2.6×1013),900×(2.6×1013)和1 200×(2.6×1013)ions/cm2的剂量注入甘草种子,M1代材料中,900×(2.6×1013)ions/cm2剂量处理对甘草具有明显刺激生长效应,而其他剂量没有这种刺激生长作用。分别把对照和900×(2.6×1013)ions/cm2剂量处理的植株进行了RAPD多态性比较。从200个引物中筛选出24个引物进行扩增,经分析可以认为,离子束注入甘草种子在基因结构上没有太多的变化,甘草的这种刺激生长效应是低能离子束物理刺激的结果。  相似文献   

应用电镜技术和血凝试验检测犬细小病毒和犬腺病毒   总被引：1，自引：0，他引：1  

李六金  押宗保  李成  姜焕宏  陈慧茹  刘建荣  马伟辉  王新建  李宏 《中国兽药杂志》2002,36(7):33-35

电镜技术和血凝试验是检测犬细小病毒和犬腺病毒快速而简便的方法。本试验结果表明,两种方法检验结果大部分相符,但各具特色。电镜技术的优势在于能够直观地显示目标病毒的生长发育、粒子的完整性以及有无非目标病毒、细菌、支原体等污染情况,这对于动物病毒疫苗种毒质量的检测是十分重要的。  相似文献   

Histological and immunohistochemical investigations of ovarian interstitial glands during non‐breeding season in camels (Camelus dromedarius)          下载免费PDF全文

MM Awad  RH Mohamed  YA Amin  HA Hussein 《Reproduction in domestic animals》2018,53(4):872-879

The aim of this was to investigate the histology and immunohistochemistry of interstitial glands during non‐breeding season in camel ovaries. A total of 21 mature, non‐pregnant and apparently healthy camels aged between 8 and 12 years were slaughtered. The ovaries were removed within 15 min, cleaned from adipose tissue, weighted and examined grossly. The histological preparation was made, and then, the blocks were cut at 3–5 microns thickness and stained by H&E for histological examinations. Moreover, some sections were stained with Sudan Black for lipid detection. Immunohistochemical analysis of paraffin‐embedded ovarian tissues was performed to detect the localization of S‐100, vimentin, progesterone receptors (PR) and oestrogen receptors (ER). Immunoreactive signals were detected using UltraVision Detection System. The results revealed that the interstitial glands were located in the cortical region and they were arranged in various arrangements either single, in couple or in groups rich in lipid droplet. All interstitial gland arrangements were enclosed by connective tissue capsules containing fibroblasts and collagenous fibres separated them from the surrounding ovarian structures. Both interstitial glands and their surrounding CT were penetrated by several blood vessels. There was a strong immunoreactive signal for S‐100 in the nuclei of interstitial cells, and no signals were detected either in cells of the interstitial glands or their connective tissue with PR. We could conclude that the interstitial gland is distinct in ovary of camel and further studies are needed to elucidate its rule in steroid synthesis.  相似文献   

Antifeedant Activity and Active Ingredients Against Plutella xylostella from Momordica charantia Leaves     

Bing LING  Associate Professor  Guo-cai WANG  Ji YA  Mao-xin ZHANG  Guang-wen LIANG 《中国农业科学(英文版)》2008,7(12):1466-1473

With the bioguided fractionation of the ethanol extracts from the leaves of Momordica charantia, we obtained two most active compounds against the feeding of the diamondback moth, Plutella xylostella larvae. The antifeedant activity of momordicine Ⅰ and momordicine Ⅱ against the second and the third instar larvae of Plutella xylostella were tested using leaf discs of cabbage in the laboratory. The results showed that momordicin Ⅰ and momordicin Ⅱ had significant antifeedant activity on the larvae of P. xylostella, and momordicin Ⅱ was more active than momordicin Ⅰ. The concentrations for 50% antifeedant effects （AFC50） of momordicin Ⅱ against the second and the third instar larvae of P. xylostella were 76.69 and 116.24μg mL^-1, whereas that of momordicin I was 144.08 and 168.42μg mL^-1, respectively. In addition, momordicin Ⅰ and momordicin Ⅱ had significant inhibitive effect on the rate of weight gain and survival of P. xylostella larvae.  相似文献   

干旱区中等肥力砂壤土棉田氮磷肥交互作用     

李磐  吉恒莹  马兴旺  杨涛 《新疆农业科学》2011,48(7):1254-1258

[目的]研究干旱区棉田相同灌水条件下,不同氮磷施肥配比对棉花产量的影响,为棉花高产合理施肥与平衡施肥提供一定的理论依据.[方法]采用正交饱和实验设计,设计不同氮、磷施肥水平及其组合,共计27个试验小区.[结果]确定了多项式回归的棉花(皮棉)产量(Y)和氮(N)和磷(P)数量的回归方程:Y=206.58 +3.21XN+9.83 XP-0.0035 XN2+ 0.029 Xp2-0.011XNXP,得出最佳施肥量:施氮量260.74 kg/hm2、施磷量120.07kg/hm2时棉花(皮棉)有最高产量Y=1 214.98 kg/hm2.[结论]不同的氮和磷的施肥水平对棉花产量的影响不同,氮和磷的交互作用最明显.  相似文献   

苦瓜叶提取物对小菜蛾的拒食活性及有效成分研究   总被引：3，自引：0，他引：3  

凌冰  王国才  轧霁  张茂新  梁广文 《中国农业科学》2008,41(10):3116-3122

【目的】从苦瓜叶乙醇提取物中分离鉴定活性成分苦瓜素Ⅰ和苦瓜素Ⅱ,测定其对小菜蛾幼虫的拒食和抑制生长发育活性。【方法】用石油醚、乙酸乙酯、正丁醇和水依次对苦瓜叶片乙醇提取物进行萃取,结果发现乙酸乙酯萃取物的拒食活性最强。活性组分经柱层析和高效液相色谱等方法分离纯化,并通过核磁共振谱(NMR)和质谱（MS）鉴定出化合物的分子结构。【结果】苦瓜素Ⅰ和苦瓜素Ⅱ对小菜蛾2、3龄幼虫的取食有明显的抑制作用。其中,苦瓜素Ⅱ的活性最高,对小菜蛾2、3龄幼虫的拒食中浓度（AFC50）分别为76.69μg•ml-1和116.24μg•ml-1。苦瓜素Ⅰ的活性次之,对小菜蛾2、3龄幼虫的AFC50分别为144.08μg•ml-1和168.42μg•ml-1。同时,苦瓜素I和苦瓜素Ⅱ对小菜蛾幼虫体重增长和存活也有抑制作用。【结论】苦瓜素Ⅱ和苦瓜素Ⅰ对小菜蛾幼虫有很强的拒食作用和抑制生长发育的作用,这些化合物的阐明对理解植物与昆虫的相互关系及其对小菜蛾的控制潜力有重要意义。  相似文献