Serum antibody responses of cats to soluble whole cell antigens of feline Porphyromonas gingivalis     

Norris JM  Love DN 《Veterinary microbiology》2000,73(1):37-49

The whole cell soluble antigens of two strains (VPB 3457 and VPB 3492) of feline Porphyromonas gingivalis were analysed by Western blotting using serum taken from 40 domestic cats with various grades of periodontal disease. Five strongly immunogenic protein bands (70, 34, 27, 24 and 19kDa) from VPB 3457 and seven from VPB 3492 (58, 44, 34, 27, 25, 24 and 21kDa) were selected for further study. A significant positive correlation was found between the serum antibody response to the 70, 34, 27, 24 and 19kDa bands of VPB 3457 and the 58, 44, 25, 24 and 21kDa bands of VPB 3492 and the overall periodontal grade. A significant positive correlation was also found between the serum antibody response to the 24kDa band of VPB 3457 and the total colony forming units of P. gingivalis. N-terminal sequencing of the 44kDa band of VPB 3492 showed 75% identity with the translated amino acids from the hag A (haemagglutinin) gene of a human strain of P. gingivalis and N-terminal amino acid sequence of the 27kDa band of VPB 3457 showed 88% identity with the amino acid sequences translated from DNA of purported genes coding for variously named proteinases of human strains of P. gingivalis.  相似文献   

Equine herpesvirus 1 (EHV-1) glycoprotein D DNA inoculation in horses with pre-existing EHV-1/EHV-4 antibody     

Ruitenberg KM  Love DN  Gilkerson JR  Wellington JE  Whalley JM 《Veterinary microbiology》2000,76(2):117-127

We have shown previously that equine herpesvirus 1 (EHV-1) glycoprotein D (gD) DNA elicited protective immune responses against EHV-1 challenge in murine respiratory and abortion models of EHV-1 disease. In this study, 20 horses, all with pre-existing antibody to EHV-4 and two with pre-existing antibody to EHV-1, were inoculated intramuscularly with three doses each of 50, 200 or 500microg EHV-1 gD DNA or with 500microg vector DNA. In 8 of 15 horses, inoculation with EHV-1 gD DNA led to elevated gD-specific antibody and nine horses exhibited increased virus neutralising (VN) antibody titres compared to those present when first inoculated. A lack of increase in gC-specific antibody during the 66 weeks of the experiment showed that the increase in gD-specific antibodies was not due to a natural infection with either EHV-1 or EHV-4. The increase in EHV-1 gD-specific antibodies was predominantly an IgGa and IgGb antibody response, similar to the isotype profile reported following natural EHV-1 infection.  相似文献   

Immunization of BALB/c mice with DNA encoding equine herpesvirus 1 (EHV-1) glycoprotein D affords partial protection in a model of EHV-1-induced abortion     

Walker C  Ruitenberg KM  Love DN  Millar Whalley J 《Veterinary microbiology》2000,76(3):211-220

DNA-mediated immunization was assessed in a murine model of equine herpesvirus 1 (EHV-1) abortion. Whilst there are differences between the model and natural infection in the horse, literature suggests that EHV-1 infection of pregnant mice can be used to assess the potential ability of vaccine candidates to protect against abortion. Female BALB/c mice were inoculated twice, 4 weeks apart, with an expression vector encoding EHV-1 glycoprotein D (gD DNA). They were mated 15 days after the second inoculation, challenged at day 15 of pregnancy and killed 3 days later. The gD DNA-inoculated mice had fewer foetuses which were damaged or had died in utero (6% in gD DNA, 21% vector DNA and 28% in nil inoculated groups challenged with EHV-1), a reduction in the stunting effect of EHV-1 infection on foetuses (gD DNA: 0.40g+/-0.06, vector DNA: 0.34g+/-0.10), reduced placental and herpesvirus-specific lung histopathology and a lower titre of virus (TCID(50)+/-SEM/lung) in maternal lung than control groups (gD DNA 4.7+/-0.3, vector 5.3+/-0.2, nil 5.6+/-0.2). Maternal antibody to EHV-1 gD was demonstrated in pups born to a dam inoculated 123 days earlier with gD DNA. Although protection from abortion was incomplete, immunization of mice with gD DNA demonstrated encouragingly the potential of this vaccine strategy.  相似文献   

Century Russet: A high-yielding fresh market cultivar with verticillium resistance     

A. R. Mosley  S. R. James  K. A. Rykbost  D. C. Hane  C. E. Stanger  C. C. Shock  J. J. Pavek  D. L. Corsini  J. C. Miller  S. L. Love  R. E. Thornton  D. G. Holm  R. E. Voss 《American Journal of Potato Research》2000,77(3):161-165

Century Russet, a high-yielding, late-maturing variety suitable for fresh tablestock use, was jointly released in 1995 by Agricultural Experiment Stations in Oregon, Idaho, Washington, Colorado, California, and Texas and the USDA Agricultural Research Service. Extremely high yields have been observed for Century Russet in variety trials conducted in Oregon and throughout the western United States under a wide range of soil and climatic conditions. Century Russet produced 40 percent higher marketable yields than Russet Burbank and 67 percent higher marketable yields than Russet Norkotah in 11 regional trials conducted over a three-year period in sevenwestern states. Uniform tuber shape, high yields of count carton sizes, and attractive tuber appearance make Century Russet well suited for the fresh market industry. It is not recommended for chipping or processing into frozen products because of low solids and dark fry color. Century Russet is highly resistant toVerticillium wilt and consistently develops less hollow heart, brown center, and other internal defects than Russet Burbank. It requires more time between vine kill and harvest than Russet Burbank for adequate tuber maturity and skin set. Bruised Century Russet tubers are susceptible to early blight andFusarium infection.  相似文献   

Umatilla Russet: A full season long Russet for processing and fresh market use     

A. R. Mosley  S. R. James  D. C. Hane  K. A. Rykbost  C. C. Shock  B. A. Charlton  J. J. Pavek  S. L. Love  D. L. Corsini  R. E. Thornton 《American Journal of Potato Research》2000,77(2):83-87

Umatilla Russet, a moderately late maturing variety especially suitable for frozen French fry processing but also acceptable for tablestock use (boiling, and baking), was jointly released by the Agricultural Experiment Stations of Oregon, Idaho, and Washington and the U.S. Department of Agriculture in 1998. Umatilla Russet was evaluated in irrigated trials in Oregon from 1988 to 1997, and in western regional trials from 1989 to 1991. Total yields were similar to those of Russet Burbank, but Umatilla Russet produced higher marketable yields. In three years of regional trials at up to 13 locations in seven western states, Umatilla Russet averaged 40.5 Mg/ha compared with 32.4 Mg/ha for Russet. Burbank. Umatilla Russet fry color and specific gravity are consistently equal to or better than for Russet Burbank. Umatilla Russet is less susceptible toVerticillium wilt than Russet Norkotah, less susceptible to net necrosis than Russet Burbank, and resistant to PVX. It is susceptible to PLRV and expresses foliar symptoms of PVY more clearly than Russet Norkotah. Umatilla Russet is less susceptible to tuber infection and decay caused byPhytophthora infestans than Ranger Russet and Russet Norkotah. Umatilla Russet is less susceptible to hollow heart, brown center, growth cracks, and sugar ends, but more susceptible to blackspot and shatter bruise than Russet Burbank  相似文献   

The effect of zinc on stallion sperm motility     

L. Vivani  C.C. Love  D.D. Varner  K. Hinrichs 《Journal of Equine Veterinary Science》2014

  相似文献   

Aortic sinus aneurysm communicating with the main pulmonary artery,and a concurrent patent ductus arteriosus,in a dog     

B. A. Love  F. Thierry  T. Schwarz  Y. Martinez-Pereira  G. J. Culshaw 《The Journal of small animal practice》2021,62(4):300-304

This is the first report of an aortic sinus aneurysm with a communication to the main pulmonary artery, resulting in left-to-right shunting, diagnosed in vivo in a dog. There was also a second left-to-right shunt through a patent ductus arteriosus. Computed tomography (CT) angiography was used to confirm both congenital anomalies and assess the relative contributions of the two left-to-right shunts to left-sided volume overload.  相似文献   

Advances in application of climate prediction in agriculture     

G. L. Hammer  J. W. Hansen  J. G. Phillips  J. W. Mjelde  H. Hill  A. Love  A. Potgieter 《Agricultural Systems》2001,70(2-3):515-553

Agricultural ecosystems and their associated business and government systems are diverse and varied. They range from farms, to input supply businesses, to marketing and government policy systems, among others. These systems are dynamic and responsive to fluctuations in climate. Skill in climate prediction offers considerable opportunities to managers via its potential to realise system improvements (i.e. increased food production and profit and/or reduced risks). Realising these opportunities, however, is not straightforward as the forecasting skill is imperfect and approaches to applying the existing skill to management issues have not been developed and tested extensively. While there has been much written about impacts of climate variability, there has been relatively little done in relation to applying knowledge of climate predictions to modify actions ahead of likely impacts. However, a considerable body of effort in various parts of the world is now being focused on this issue of applying climate predictions to improve agricultural systems.

In this paper, we outline the basis for climate prediction, with emphasis on the El Niño-Southern Oscillation phenomenon, and catalogue experiences at field, national and global scales in applying climate predictions to agriculture. These diverse experiences are synthesised to derive general lessons about approaches to applying climate prediction in agriculture. The case studies have been selected to represent a diversity of agricultural systems and scales of operation. They also represent the on-going activities of some of the key research and development groups in this field around the world. The case studies include applications at field/farm scale to dryland cropping systems in Australia, Zimbabwe, and Argentina. This spectrum covers resource-rich and resource-poor farming with motivations ranging from profit to food security. At national and global scale we consider possible applications of climate prediction in commodity forecasting (wheat in Australia) and examine implications on global wheat trade and price associated with global consequences of climate prediction.

In cataloguing these experiences we note some general lessons. Foremost is the value of an interdisciplinary systems approach in connecting disciplinary knowledge in a manner most suited to decision-makers. This approach often includes scenario analysis based on simulation with credible models as a key aspect of the learning process. Interaction among researchers, analysts and decision-makers is vital in the development of effective applications — all of the players learn. Issues associated with balance between information demand and supply as well as appreciation of awareness limitations of decision-makers, analysts, and scientists are highlighted. It is argued that understanding and communicating decision risks is one of the keys to successful applications of climate prediction.

We consider that advances of the future will be made by better connecting agricultural scientists and practitioners with the science of climate prediction. Professions involved in decision making must take a proactive role in the development of climate forecasts if the design and use of climate predictions are to reach their full potential.  相似文献   

Is the endangered Fennoscandian arctic fox (Alopex lagopus) population genetically isolated?     

Love DalénAnders Götherström  Magnus TannerfeldtAnders Angerbjörn 《Biological conservation》2002,105(2):171-178

The arctic fox population in Fennoscandia is on the verge of going extinct after not being able to recover from a severe bottleneck at the end of the 19th century. The Siberian arctic fox population, on the other hand, is large and unthreatened. In order to resolve questions regarding gene flow between, and genetic variation within the populations, a 294 bp long part of the mitochondrial hypervariable region 1 was sequenced. This was done for 17 Swedish, 15 Siberian and two farmed foxes. Twelve variable nucleotide sites were observed, which resulted in 10 different haplotypes. Three haplotypes were found in Sweden and seven haplotypes were found in Siberia. An analysis of molecular variance showed a weak, but significant, differentiation between the populations. No difference in haplotype diversity was found between the populations. A phylogenetic analysis revealed that the three Swedish haplotypes were not monophyletic compared to the Siberian haplotypes. These results indicate a certain amount of gene flow between the two populations, both before and after the bottleneck. Restocking the Fennoscandian population with arctic foxes from Siberia might therefore be a viable option.  相似文献   

Induced mutations for reduced tuber glycoalkaloid content in potatoes   总被引：1，自引：0，他引：1  

S. L. Love    T. P. Baker    A. Thompson-Johns  B. K. Werner 《Plant Breeding》1996,115(2):119-122

Mutation breeding was used to produce mutants of the potato breeding selection NDA1725-1 with reduced levels of tuber glycoalkaloids. Excised tuber eye-pieces were exposed to 35 Gy of gamma rays from a ⁶⁰Co source. In the M₁V₁, leaf tissue was screened using high-performance thin layer chromatography and each plant given a relative score based on a subjective rating of band intensity. Tubers were harvested from all plants that produced leaves with relatively low levels of glycoalkaloids. Tuber glycoalkaloids were quantified in the M₁V₂ and M₁V₃ using high-performance liquid chromatography, and 35% and 23% of clones with the lowest glycoalkaloid content were selected for further evaluation in each generation, respectively. In the M₁V₄, M₁V₅ and M₁V₆, selection was based on statistically significant differences between the mutant clones and the controls for glycoalkaloid content, as determined using a gravimetric quantification method, as well as on agronomic and quality characteristics. At the end of six clonal generations three selections exhibited acceptable levels of tuber glycoalkaloids and retained enough of the parent clone's exceptional traits to have potential in the commercial potato chip (crisp) industry.  相似文献