Application of congultinating complement adsorption test for detecting antibodies from the natural cases of bovine brucellosis     

T S Gulrajani  R P Misra  J C Verma 《The Indian veterinary journal》1968,45(1):18-21

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Optimizing Koi Carp,Cyprinus carpio var. Koi (Linnaeus, 1758), Stocking Density and Nutrient Recycling With Spinach in an Aquaponic System     

T. Hussain  A. K. Verma  V. K. Tiwari  C. Prakash  G. Rathore  A. P. Shete  K. K. T. Nuwansi 《Journal of the World Aquaculture Society》2014,45(6):652-661

Fish waste water nutrient recycling in an aquaponic system was studied under different stocking densities of Koi Carp, Cyprinus carpio var. koi, along with spinach, Beta vulgaris var. bengalensis. Fish growth performance, plant growth, nutrient dynamics, and nutrient removal and their dependence on different stocking densities, namely 1.4, 2.1, and 2.8, were observed, of the different combinations, fish stocked at 1.4 kg/m³ had the best growth. Percent nutrient removal (NO₃–N, PO₄–P, and K) was significantly higher at 1.4 kg/m³. Thus, 1.4 kg/m³ stocking density can be suggested as optimum for Koi Carp production in spinach aquaponic systems.  相似文献   

In vitro and in vivo pathogenicity studies of Pasteurella multocida strains harbouring different ompA   总被引：1，自引：0，他引：1  

Shailja Katoch  Mandeep Sharma  R. D. Patil  Sandeep Kumar  Subhash Verma 《Veterinary research communications》2014,38(3):183-191

Pasteurella multocida is a pathogenic, Gram-negative bacterium that is commonly found as normal flora in nasopharynx of variety of wild and domestic animals. Numerous virulence factors have been described for P. multocida isolates which include adherence and colonization factors, iron-regulated and acquisition proteins, extracellular enzymes such as neuraminidase, lipopolysaccharide (LPS), capsule and a variety of outer membrane proteins (Omp). OmpA has a significant role in stabilizing the cell envelope structure by providing physical linkage between the outer membrane & peptidoglycan. It has been shown to mediate P. multocida -host cells interaction via heparin and/or fibronectin binding and therefore act as an important invasive molecule which could determine the final outcome of initial infection. Comparative nucleotide sequence analysis of ompA gene of P. multocida has revealed that despite extensive genetic diversity in ompA of P. multocida, most sequences could be classified into two major allele classes namely ompA allele (I) and allele (II). The P. multocida recovered from nasal cavity of bovine and belonging to two ompA classes were tested for their differential virulence. In vitro pathogenicity studies on Madin Darby Bovine Kidney (MDBK) cell line employing adhesion and invasion assays indicated that P. multocida strain with ompA (I) is more invasive than P. multocida strain with ompA (II). In vivo studies in mice further reiterated that the isolates harbouring ompA(I) were comparatively more virulent to isolates harbouring ompA (II).  相似文献   

Expression cloning of human EGF receptor complementary DNA: gene amplification and three related messenger RNA products in A431 cells   总被引：45，自引：0，他引：45  

C R Lin  W S Chen  W Kruiger  L S Stolarsky  W Weber  R M Evans  I M Verma  G N Gill  M G Rosenfeld 《Science (New York, N.Y.)》1984,224(4651):843-848

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Identification of a transcriptional enhancer element upstream from the proto-oncogene fos   总被引：41，自引：0，他引：41  

J Deschamps  F Meijlink  I M Verma 《Science (New York, N.Y.)》1985,230(4730):1174-1177

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Effect of sublethal concentrations of mercury on the composition of liver,muscles and ovary of Notopterus notopterus     

S. R. Verma  I. P. Tonk 《Water, air, and soil pollution》1983,20(3):287-292

The present investigation was undertaken to evaluate the effect of sublethal concentrations (0.088 and 0.044 mg 1^?1, i.e., 1/5th and 1/10th fraction of 96 h LC₅₀) of mercuric chloride on Ca, inorganic phosphate, water content, ash, glycogen, Fe, total protein, lipid contents and vitamin A, C, D, and E of liver, muscles and ovary of the freshwater fish Notopterus notopterus exposed for 30 days. The results obtained indicate that Ca, inorganic phosphate, water, ash, glycogen, Fe, total protein contents and vitamin D decreased significantly or insignificantly in these tissues of the Hg exposed fishes. However, lipid content increased in liver and muscles, but decreased in the ovaries. Vitamin A and D increased in the liver but decreased in the muscles and ovaries. Moreover, vitamin E increased in all the tissues of the fish exposed to 0.088 mg 1^?1 and decreased in the fish exposed to 0.044 mg 1^?1 of Hg concentrations.  相似文献   

Characterization of the interspecific cross Cicer anetinum L. ×C. judaicum (Boiss)     

M. M. Verma    Ravi  J. S. Sandhu 《Plant Breeding》1995,114(6):549-551

An interspecific cross involving Cicer anetinum L. and C. judaicum (Boiss) No. 182 was established. The F₁ could be distinguished from the parents by its prostrate growth habit at the seedling stage and through isozyme patterns for peroxidases and esterases. The inheritance of PRX-3 was found to be relatively simple and behaved as a monomer. Meiotic studies indicated the occurrence of six bivalents and four uni-valents in about 6% of F₁ PMC's at metaphase I, but normal 8–8 chromosomal distribution in anaphase I, indicating near complete homology. The F₁ hybrid was characterized for three morphological and six agronomical characters. A large F₂ was studied for secondary branches per plant, 100-seed weight (g), pods per plant, and grain yield per plant (g). Distributions of F₂ for pod number and grain yield displayed high C.V. and were highly skewed in a positive direction. F₂ recombinants were isolated with a very large number of secondary branches and a high pod number and yield. Such wide variability is not normally encountered in intevarietal crosses.  相似文献   

Identification and characterization of quantitative trait loci related to lodging resistance and associated traits in bread wheat   总被引：2，自引：0，他引：2  

V. Verma    A. J. Worland    E. J. Savers    L. Fish    P. D. S. Caligari  J. W. Snape 《Plant Breeding》2005,124(3):234-241

Lodging is a major constraint to increasing yield in many crops, but is of particular importance in the small‐grained cereals. This study investigated the genetic control of lodging and component traits in wheat through the detection of underlying quantitative trait loci (QTL), The analysis was based on the identification of genomic regions which affect various traits related to lodging resistance in a population of 96‐doubled haploid lines of the cross ‘Milan’בCatbird’, mapped using 126‐microsatellite markers. Although major genes related to plant height (Rht genes) were responsible for increasing lodging resistance in this cross, several other traits independent of plant height were shown to be important such as fool and shoot traits, and various components of plant yield. Yield components such as grain number and weight were shown to be an indicator of plant susceptibility to lodging. QTL for lodging and associated traits were found on chromosomes IB, ID. 2B. 2D. 4B, 4D. 6D and 7D. QTL for yield and associated traits were identified on chromosomes IB, ID. 2A. 2B. 2D. 4D and 6A,  相似文献   

Recent advances in carp seed production and milt cryopreservation     

P. Routray  D. K. Verma  S. K. Sarkar  N. Sarangi 《Fish physiology and biochemistry》2007,33(4):413-427

The fish-seed production industry in India has recorded remarkable growth over the last three decades. The hypophysation technique was successfully introduced into India in 1957 and steady progress towards the refinement of the technique has been registered, which has revolutionized carp seed production in the Indian subcontinent. Advancement of carp maturity through brood stock management and multiple breeding has enabled spawn production well ahead of the monsoon and even beyond, ensuring seed availability throughout the year. The quality of seed is an important consideration for commercial aquaculturists. So, partial stock replenishment in carp hatcheries is practised to overcome the problem of inbreeding, which otherwise leads to poor growth of carps. Similarly, gametes of improved stock are cryopreserved and utilized for quality seed production as well as upgrading the brood stock of carps. The gamete cryopreservation protocol for carps is the focus of this review. An attempt has also been made to incorporate information on carp brood stock management, inducing agents, and seed production, including hatchery management.  相似文献   

Forage production and nitrogen nutrition in three grasses under coconut tree shades in the humid-tropics     

C.?B.?PandeyEmail author  S.?K.?Verma  J.?C.?Dagar  R.?C.?Srivastava 《Agroforestry Systems》2011,83(1):1-12

Reduction in forage production (FP) under trees in the humid tropics is well known, but information on how different levels of nitrogen (N) fertilizer influence FP under trees is meager. The present study reports effects of four N fertilizer levels (0, 60, 80 and 120 kg ha⁻¹ N) on net soil N mineralization rate (NMR) and soil moisture (SM), FP, shoot biomass/root biomass ratio (SB/RB), N concentration in SB, N uptake and nitrogen use efficiency (NUE) of three grasses [guinea (Panicum maximum Jacq.), para (Brachiaria mutica (Forssk) Stapf) and hybrid-napier (Pennisetum purpureum Schumach.)] under three canopy positions [under canopy (UC, representing high shade), between canopy (BC, representing low shade) and open] of coconut trees (Cocos nucifera L.) in a coconut based silvopastoral system in the humid tropical climate of South Andaman Island of India. The study was performed for two annual cycles (2005–2006 and 2006–2007). The hypotheses tested were: (1) FP would decline under tree shades, both in N fertilized as well as no N fertilized conditions, when SM was not growth limiting in the open. However, amount of decline in the FP would depend on grass species and intensity of shades i.e., higher was the shade greater would be the decline; (2) N fertilizer would increase FP under tree shades, but the increase depended on grass species, intensity of shades and amount of N applied. Amount of N applied, however, would not annul the shades effects when SM was not growth limiting in the open. The study revealed that the tree reduced light 59% under UC and 32% under BC positions, but the N fertilizer levels increased NMR by 11–51% under UC and 3–44% under BC positions compared to the open. SM did not differ across the canopy positions. Under all situations, FP of all grasses declined under UC (47–78%) and BC (18–32%) positions compared to the open; the decline was greater in Hybrid-napier than Guinea and Para grasses. Forage production of all grasses increased with N fertilizer increments under all canopy positions reaching 32 t ha⁻¹ dry matters for hybrid-napier at 120 kg ha⁻¹ N in the open. Both guinea and para grasses outyielded hybrid-napier grass under UC but not under BC or in the open. N concentration in the forage (SB) also increased as N fertilizer level increased. These observations support our hypotheses and suggest that forage production under coconut palms can be increased by the application of N fertilizer with both guinea and para grasses being more productive than hybrid-napier grass under the high shade. Where light conditions are better, hybrid-napier would produce more forage than the other species.  相似文献