Interactions of organic and inorganic chromium species with Ca-polygalacturonate     

Tanja Mimmo  Luciano Cavani  Riccardo Reggiani  Claudio Marzadori  Carlo Gessa 《Biology and Fertility of Soils》2008,44(3):521-526

This study investigated the interactions of CrIII and CrVI present as free metal ions or as organic CrIII (Cr-L) with a Ca-polygalacturonate (Ca-PG) network. Ca-PG networks were treated with solutions of chromium (CrIII and CrVI) at different concentrations at three different pH values. The Cr-L complex were obtained by water extraction of hydrolysed leather. The CrVI had almost no interaction with the Ca-PG networks, whereas CrIII as a free metallic ion was strongly retained by the network. The calculated Langmuir adsorption isotherms showed a twofold increase in maximum CrIII adsorption but a decrease in the affinity of the metal with the Ca-PG networks. This would indicate that the different chromium species, i.e. CrIII and its hydrolytic species, interact in a different way with the polygalacturonate chains. In addition, thermal analysis showed that CrIII sorption modified the structure of the network. These modifications were reduced, and thermal stability increased if Ca-PG adsorbed Cr-L or L. Furthermore, the treatment of Ca-PG with hydrolysed leather water extract-reduced CrIII sorption by 94%. The hydrolysed leather fraction immobilized onto the Ca-PG networks probably consisted of peptides and could therefore represent an important source of organic N.  相似文献   

Photosensitizing properties of protein hydrolysate-based fertilizers     

Cavani L  Ter Halle A  Richard C  Ciavatta C 《Journal of agricultural and food chemistry》2006,54(24):9160-9167

The use of protein hydrolysate-based fertilizers (PHF) as adjuvant for pesticides or herbicides has been proposed; however, the behaviors of mixtures of PHFs and pesticides under solar light are not known, and various photochemical reactions may occur. The photosensitizing properties of PHFs were investigated in water solutions (0.8 g of total organic carbon L(-1)) within the wavelength range of 300-450 nm, using furfuryl alcohol (FA) as a probe to test the involvement of singlet oxygen and Irgarol 1051 as an example of organic pollutant. Two commercial PHFs and one standard PHF were studied, all of the products being of animal origin. PHFs photosensitize the transformation of FA (10(-4) M), and the kinetics of FA disappearance follows an apparent first-order rate law. Through the use of sodium azide (1 x 10(-3) M) as singlet oxygen scavenger and deuterium oxide (D2O) for increasing the singlet oxygen lifetime it was shown that singlet oxygen contributes largely to the phototransformation of FA. The replacement of water by D2O increases the apparent first-order rate constant 6 times, whereas the addition of sodium azide reduces it by approximately 90%. These results are confirmed using Irgarol 1051 (10(-5) M). The photosensitizing properties of PHFs might be due to pigments naturally present in tissues from which they are extracted or to compounds generated during the production processes.  相似文献   

Oxidative stress and antioxidant responses in juvenile Brazilian flounder <Emphasis Type="Italic">Paralichthys orbignyanus</Emphasis> exposed to sublethal levels of nitrite     

Lucas Campos Maltez  Luis André Luz Barbas  Lilian Fiori Nitz  Lucas Pellegrin  Marcelo Hideo Okamoto  Luís André Sampaio  José Maria Monserrat  Luciano Garcia 《Fish physiology and biochemistry》2018,44(5):1349-1362

This study evaluated the effects of short-term exposure to sublethal levels of nitrite on oxidative stress parameters and histology of juvenile Brazilian flounder Paralichthys orbignyanus. An assessment of fish recovery was also performed. Fish were exposed to 0.08 (control), 5.72, 10.43, and 15.27 NO₂-N mg L^?1 for 10 days followed by the same recovery time. Gill, liver, and muscle samples were collected after 1, 5, and 10 days of exposure and after recovery for the measurement of antioxidant capacity against peroxyl radicals (ACAP), glutathione-S-transferase (GST) activity, content of non-protein (NPSH) and protein thiols (PSH), and lipid peroxidation levels by thiobarbituric acid-reactive substances (TBARS) content. Nitrite exposure induced alterations which compromised the overall antioxidant system (reduced ACAP and GST activity) and enhanced oxidative damage in lipids and proteins. Increases in GST activity and NPSH and PSH contents were also demonstrated. The recovery period allowed for resumption of basal levels for all (treatment 5.72 NO₂-N mg L^?1) or some of the evaluated parameters (other treatments). In conclusion, exposure to nitrite concentrations from 5.72 to 15.27 NO₂-N mg L^?1 induced oxidative stress and antioxidant responses in juvenile Brazilian flounder. The 10-day recovery period was sufficient for a complete resumption of basal physiological condition of fish exposed to concentrations of up to 5.72 NO₂-N mg L^?1.  相似文献   

Presence of <Emphasis Type="Italic">Ureaplasma diversum</Emphasis> in the genital tracts of female dairy cattle in Mato Grosso State,Brazil     

Jaqueline B. Azevedo  Gustavo S. Silva  Priscylla S. Rocha  Letícia C. Pitchenin  Valéria Dutra  Luciano Nakazato  Anderson Castro Soares de Oliveira  Caroline A. Pescador 《Tropical animal health and production》2017,49(2):311-316

Ureaplasma diversum infection in bovine females may result in various reproductive problems, including granular vulvovaginitis, abortion, weak calves, salpingitis, and spontaneous abortion. The presence of U. diversum in a dairy bovine population from midwestern Brazil has not been established. The aim of this study was to determine whether U. diversum was present in dairy cattle from midwestern Brazil using polymerase chain reaction (PCR). Vulvovaginal mucus was analyzed from 203 cows located in six municipalities in the north region of Mato Grosso State, Brazil. A total of 25% of dairy cows with vulvovaginitis were positive for U. diversum. The factors evaluated were included in a multivariable logistic regression model with the presence of at least one positive cow in the herd serving as the dependent variable. Three variables were significantly associated with a U. diversum-positive PCR and were included in the final multivariable model: number of parities, vulvar lesions, and reproductive problems. For each new parity, the chance of U. diversum infection decreased 0.03-fold, indicating that cows with the highest number of parities were more protected. The presence of vulvar lesions was increased 17.6-fold in females positive for U. diversum, suggesting that this bacterium could be related to the red granular lesions in the vulvar mucosa, whereas reproductive problems were increased 7.6-fold. However, further investigations should be conducted to ascertain the effects of U. diversum in association with other mycoplasma species in the herds studied.  相似文献   

Phenolic Compounds Determined by LC-MS/MS and <Emphasis Type="Italic">In Vitro</Emphasis> Antioxidant Capacity of Brazilian Fruits in Two Edible Ripening Stages     

Fabiana Della Betta  Priscila Nehring  Siluana Katia Tischer Seraglio  Mayara Schulz  Andressa Camargo Valese  Heitor Daguer  Luciano Valdemiro Gonzaga  Roseane Fett  Ana Carolina Oliveira Costa 《Plant foods for human nutrition (Dordrecht, Netherlands)》2018,73(4):302-307

The aim of this study was to investigate the free individual phenolics and the in vitro antioxidant capacity of blackberry, acerola, yellow guava, guabiju, jambolan and jabuticaba fruits in two edible stages. Of the thirty-three phenolics investigated by liquid chromatography - tandem mass spectrometry (LC-MS/MS), twenty-five were quantified and the major ones were catechin, isoquercitrin, epicatechin and gallic acid. The highest values for the total phenolic content (in dry matter) were observed for acerola (83.6 to 97.7 mg gallic acid equivalents g^?1 DM) and blackberry (18.9 to 28.3 mg gallic acid equivalents g^?1 DM); however, acerola, jabuticaba, and blackberry showed the highest antioxidant capacities (134.6 to 1120.4 mg Trolox equivalents g^?1 for 2,2-diphenyl-1-picrylhydrazyl and 43.6 to 501.8 μmol Trolox equivalents g^?1 for ferric reducing antioxidant power). For most fruits, the antioxidant capacity decreased during the ripening, possibly due to a decrease in the concentration of most of the phenolics.  相似文献   

Local diversity of native arbuscular mycorrhizal symbionts differentially affects growth and nutrition of three crop plant species     

Alessandra?TurriniEmail author  Alberico?Bedini  Mario?Bonilla?Loor  Gaia?Santini  Cristiana?Sbrana  Manuela?Giovannetti  Luciano?AvioEmail author 《Biology and Fertility of Soils》2018,54(2):203-217

Intact whole native AMF communities occurring across a 100-m-long field were used for the evaluation of plant performance, as determined by the actual fungal species colonizing host roots. The soil from distinct plots within a “hot spot” field was collected to set up 54 experimental units where three different plant species were grown, in order to test whether the whole native AMF communities were able to differentially affect plant growth, to assess the genetic identity of the AMF actually colonizing the tested plants and to analyse their community composition in the different hosts. Molecular analyses revealed that plant growth and nutrition of the crop plants were differentially affected by the diverse native arbuscular mycorrhizal communities colonizing the roots of the three plants, whose performance varied depending on the identity of plant hosts and fungal symbionts, more than on a rich and diversified AMF community. Such results, improving our understanding of AMF distribution at the local scale, represent a starting point allowing the selection, isolation and characterization of the most efficient AMF assemblages to be used as inoculants in sustainable food production systems.  相似文献   

Phosphorus dynamics in the rhizosphere of two wheat cultivars in a soil with high organic matter content     

Bruna Arruda  Daniel João Dall’orsoletta  Joice Crescencio Heidemann  Luciano Colpo Gatiboni 《Archives of Agronomy and Soil Science》2018,64(7):1011-1020

Soil organic matter (SOM) is a key of most nutrient cycling and its content influences labile phosphorus (P) pool. In order to promote P availability from SOM, some plant strategies could be important to increase organic P mineralization, which may change among cultivars of the same crop. This study evaluated phosphorus dynamics in the rhizosphere of two wheat cultivars in soil with high organic matter content. Experiment was established in a greenhouse using a Humic Cambisol in a randomized block design using Quartzo and Abalone as wheat cultivars and harvested 20 days after seedling. Pots with a horizontal mesh (25 µm) were used to isolate the soil from roots. At harvest, the soil under the mesh was sliced in five distances from the rhizoplane (0–3; 3–6; 6–10; 10–20; 20–30 mm). Organic P was the buffer to maintain P dynamics in the rhizosphere and there was phosphorus depletion in the first slice near the rhizoplane due to the root effect, regardless the cultivar. Quartzo showed high labile inorganic P, presumably due to the high amount of root hairs, which increased the acid phosphatase activity and consequently root P uptake. Quartzo was more efficient in changing rhizosphere regarding the P acquisition.  相似文献   

Effect of Long-Term Sugarcane (Saccharum Spp.) Cultivation on Chemical and Physical Properties of Soils in Belize     

Luciano Chi  Jorge Mendoza-Vega  Esperanza Huerta  José David Álvarez-Solís 《Communications in Soil Science and Plant Analysis》2017,48(7):741-755

The sugarcane industry in Belize is one of the main economic drivers in the country and is therefore of crucial social and environmental importance. This study evaluated the degree of sustainability of commercial sugarcane production in Northern Belize by determining soil parameters (physical and chemical) in three soil layers (0–15, 15–30 and 30–50 cm) and crop profitability relative to years of sugarcane cultivation (4–25 years since land conversion). The parameters evaluated were organic matter (OM), total nitrogen (TN), cation exchange capacity (CEC), available phosphorus (P), potassium (K), pH, dry bulk density (DBD), porosity (?), and water-filled pore space (WFPS). Field interviews were conducted to document management practices, agricultural inputs, production costs, and yield. The results showed that OM and TN in all soil layers studied and CEC in the 30–50 cm layer decreased, and were negatively correlated, with years of sugarcane cultivation. This indicates that prolonged sugarcane cropping has detrimental effects on soil fertility. There was no clear pattern with years under sugarcane cultivation for P, pH, DBD, and K. Yield levels were maintained by intensification of cultivation, e.g. high inputs and regular replanting, providing short-term benefits at the expense of deterioration of soil fertility. The benefit to cost (B:C) ratio of sugarcane production in Northern Belize was marginal for American Sugar Refinery/Belize Sugar Industries (ASR/BSI), representative of intensive agriculture; and not profitable for small-scale farmers, 1.0 and 0.63 for plantation establishment and 1.2 and 1.0 average for the following six years of ratoon, respectively.  相似文献   

Linking fish ecomorphotypes to food categories and local-scale habitat uses along a Brazilian coastal stream     

Luciano Lazzarini Wolff  Edson Fontes de Oliveira  Javier Lobón-Cerviá 《Ecology of Freshwater Fish》2023,32(1):195-208

Fish ecomorphological analyses often deal with several traits simultaneously, making it challenging to interpret general patterns, and have not addressed the longitudinal component of streams in morphology-habitat relationships. We identified ecomorphotypes of fishes and correlated their morphological variation with food, structural/hydrological variables, and abundances to detect their links with diet and local-scale habitat categories. Eighteen morphometric traits were obtained from specimens collected by electrofishing in a coastal Atlantic stream in Brazil. To test for morphology-habitat associations, headwaters, middle, and mouth reaches were divided into shorter sampling sites, with the middle reach being classified into riffle, run, and pool mesohabitats. Multivariate analyses highlighted the morphological variations and associated the form categories of 18 fish species with food and habitat categories. It resulted in four combined trophic and habitat ecomorphotypes: (1) benthic AB/lithophilic, composed of smaller to longer loricariids, detritivores, of fast-water, and pebbly habitats; (2) benthic C/lithophilic of very fast-waters, algal and detritus scrapers, with longer intestines than the other benthic species, wider suctorial mouths, inhabitants of headwaters, and riffles habitats; (3) nektobenthic/lithophilic with streamlined bodies, invertebrate feeders, and dwellers of faster-water, cobbly habitats; and (4) nektonic/limnophilic composed mainly by characins, which had strong correlations with terrestrial insect consumption, and lentic/pool, sandy habitats. The morphology–environment correlations linked these ecomorphotypes to the broad habitat gradient arrayed in longitudinal and local scales. These results permit inferring the larger form patterns expected for local fish assemblages and emphasise the fish trait categorisation as a possible surrogate to reveal broader ecomorphological associations.  相似文献   

Isolation of Wheat High Molecular Weight Glutenin Subunits from Durum Wheat     

Antonella Capocchi  Luciano Galleschi  Franco Saviozzi 《Cereal Chemistry》2000,77(2):105-106

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