Results of a web-based health survey of retired racing Greyhounds   总被引：1，自引：0，他引：1  

Lord LK  Yaissle JE  Marin L  Couto CG 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(6):1243-1250

BACKGROUND: Adoption of retired racing Greyhounds has become increasingly popular during the past decade. To date, research has focused on the physiologic and clinicopathologic peculiarities of Greyhounds but there is little published information on disease prevalence in the breed. OBJECTIVE: The objective of this study is to determine the prevalence of disease in retired racing Greyhounds. ANIMALS: In this study, 747 Greyhounds were used. METHODS: A standardized survey method was used, and survey responses were collected by an Internet survey. Owners could answer a survey for every Greyhound that they had owned since January 1, 2005. RESULTS: Of the 692 eligible participants, 441 (63.7% response rate) completed surveys for 747 Greyhounds. The mortality rate for Greyhounds within the 2-year period was 15% (113 of 747 died). The most common cause of death reported was cancer (66 dogs, 58%), and the most common type of cancer listed as the cause of death was osteosarcoma (28 dogs, 25%). The most commonly reported groups of diseases or disorders were skeletal (232 dogs, 33%), skin (197 dogs, 28%), digestive (132 dogs, 18%), cancer (94 dogs, 13%), and endocrine (85 dogs, 11.9%). Forty-five percent of Greyhounds diagnosed with cancer and 6% of the overall population had osteosarcoma. CONCLUSIONS AND CLINICAL IMPORTANCE: The results of this study can be used by veterinary researchers to continue to investigate the most common diseases in this population. As more retired racing Greyhounds enter the pet population, the results of this study will help educate veterinarians and owners about the most prevalent diseases in the breed.  相似文献   

Comprehensive prediction of plant cytoplasmic and apoplastic effectors underlying Erwinia psidii pathogenicity     

Francisco Henrique Nunes da Silva Alves  Isadora Cristófoli Pereira  Samuel Alves dos Santos  Ana Leticia Rocha Monteiro  Pedro Marcus Pereira Vidigal  Acelino Couto Alfenas  Jorge Luis Badel 《Plant pathology》2023,72(1):130-143

Erwinia psidii (Eps) is the causal agent of emerging diseases of eucalypt and guava; however, the mechanisms underlying its pathogenicity are not fully understood. Here, we predicted factors involved in the ability of Eps to cause disease on its host plants. For that, the genomes of four Eps strains exhibiting different virulence on eucalypt were sequenced, and hrp/hrc genes coding for the type III secretion system (T3SS), effectors injected into the plant cell cytoplasm through the T3SS (T3SEs) and their plant subcellular localizations, as well as proteins deployed to the host apoplast, were predicted. It was found that Eps possesses a complete hrp/hrc gene cluster based on comparison with Erwinia amylovora. A total of 18 T3SEs were predicted, 11 of which were shared among all strains, none were exclusive to any strain and seven were absent in at least one strain. No sequence variation among strains was found for five T3SE candidates whereas extensive variation was found for six, suggesting the latter may be determinants of virulence differences. The T3SE candidates are predicted to target the plant cell nucleus, cytoplasm, mitochondrion, chloroplast and peroxisome. The predicted apoplastic effector repertoire common to all four strains was over-represented in proteins of unknown functions or predicted to possess enzymatic activities, among which the most abundant were oxidoreductases and peptidases. Proteins with lytic transglycosylase activity were predicted in strain-specific apoplastic effector repertoires. These results provide an important framework for future research aimed at uncovering the factors underlying Eps pathogenicity.  相似文献   

Prevalence,species distribution and antimicrobial resistance patterns of methicillin-resistant staphylococci in Lithuanian pet animals     

Modestas Ruzauskas  Natacha Couto  Sigita Kerziene  Rita Siugzdiniene  Irena Klimiene  Marius Virgailis  Constan?a Pomba 《Acta veterinaria Scandinavica》2015,57(1)

Background

The bacterial genus Staphylococcus consists of many species that causes infections in pet animals. Antimicrobial resistant staphylococci cause infections that are difficult to treat and they are important from the point of one health perspective. The aim of this study was to determine the prevalence of methicillin-resistant Staphylococcus (MRS) species, including methicillin-resistant S. aureus (MRSA) in diseased pet animals (Group A) and kennel dogs (Group B) in Lithuania and to characterize the isolates according to their antimicrobial resistance.

Results

Twenty-one MRS isolates were obtained from 395 clinical samples (5.3 %; CI 95 % 3.5-8.0) of Group A animals. Sixteen, four and one isolates were from dogs, cats and a pet rabbit, respectively. The mecA gene was present in 20 isolates, whereas one isolate was positive for the mecC gene. Twenty-one MRS isolates (20.0 %; CI 95 % 13.5-28.6) were obtained from the vagina of female dogs (n = 105) (Group B). All isolates carried the mecA gene. Twelve MRS species were isolated of which S. pseudintermedius was the most common (18/42) followed by S. haemolyticus (8/42) and S. lentus (4/42). MRSA was not found. All MRS strains were susceptible to vancomycin, linezolid, daptomycin and quinupristin/dalfopristin. Resistance to tetracycline (16/21), clindamycin (15/21) and erythromycin (14/21) was the most common types of resistance in Group A animals. Three isolates also demonstrated resistance to rifampin. Resistance toward gentamicin (16/21), ciprofloxacin (15/21), macrolides (15/21) and tetracycline (12/21) was the most common in kennel dogs (Group B). The most common genes encoding resistance to antimicrobials (excluding beta-lactams) in isolates from Group A pets were tetK (21/42), aph(3′)-IIIa (11/42) and aac(6'')-Ie-aph(2'''')-Ia (9/42).

Conclusions

A wide range of MRS species were found in pet animals in Lithuania. MRSA was not found.  相似文献   

Intake,performance, and efficiency of nutrient utilization in Saanen goat kids fed diets containing calcium salts of fatty acids     

Possamai  Ana Paula Silva  Alcalde  Claudete Regina  de Souza  Rodrigo  Gomes  Ludmila Couto  de Macedo  Francisco de Assis Fonseca  Martins  Elias Nunes 《Tropical animal health and production》2015,47(1):259-263

Tropical Animal Health and Production - The objective of this study was to evaluate the effect of feeding Saanen goat kids with calcium salts of fatty acids (CSFA) in diet, on intake, performance,...  相似文献   

Nucleotide Sequence Variability at the 5′-UTR of Potato PVY Isolates     

Arie Rosner  Ludmila Maslenin 《Potato Research》2006,49(3):217-224

Potato virus Y (PVY) isolates from potato were divided into two major groups – I and II – on the basis of nucleotide sequence variability at the 5′-UTR of the virus genome. In this paper, a consensus sequence for each group was compiled by examining PVY isolates of potato. The consensus sequences of the two groups differed from each other by as much as 31% of nucleotide sequence divergence. However, sequences of most individual members within each group (except for one, the 605 strain) were conserved and varied by less than 1% in group II and up to 6% in group I. These results confirm that the two virus groups are markedly distinct but that the 5′-UTR sequence of individual members within each group is highly conserved. These findings imply that the 5′-UTR can be reliably applied for major grouping of isolates but that its use for discrimination among individual isolates within each group is limited. Isolates of both group I and II included members of the O/N-NTN virus types. Classification of PVY isolates in potato is discussed.  相似文献   

Applying cost-distance analysis for forest disease risk mapping: Phytophthora austrocedrae as an example     

Ludmila La Manna  Alina G. Greslebin  Silvia D. Matteucci 《European Journal of Forest Research》2013,132(5-6):877-885

Cost-distance model analyzes the relative difficulty in reaching each spot of the landscape for the object or species under study. It calculates the effective distance, which is the Euclidian distance modified by the friction to movement through different landscape elements. This work deals with the application of cost-distance analysis in forest pathology, considering Austrocedrus chilensis root rot caused by Phytophthora austrocedrae as an example. In this case, cost-distance analysis was used to determine the relative difficulty for the pathogen to reach healthy forest patches from the patches that are presently diseased. Friction values were assigned on the basis of abiotic conditions, biological characteristics of the pathogen and host presence. Since cattle may be a vehicle for Phytophthora dispersion, three hypothetical situations of ranching were considered. Cost-distance application resulted useful to define minimum risk areas for conservation purposes. In the study case, minimum risk area strongly varied in response to cattle presence. This study provided valuable information for A. chilensis disease management and showed one of the broad applications of cost-distance analysis in forestry.  相似文献   

Markers of iron metabolism in retired racing Greyhounds with and without osteosarcoma     

J. T. Caro  L. M. Marín  M. C. Iazbik  S. Zaldivar‐López  H. Borghese  C. G. Couto 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2013,42(3):360-363

  相似文献   

In Vitro Digestibility of Different Commercial Edible Algae Products     

Ludmila Machů  Dušan Samek  Jan Hrabě  Miroslav Fišera 《Journal Of Aquatic Food Product Technology》2013,22(5):423-435

Digestibility is an important factor in determining the level of nutritive factors utilization. Determination of in vitro digestibility in a Daisy incubator and assessment of protein digestibility using Kjeldahl method of algal food products after pepsin, pancreatin, and combined hydrolysis of both enzymes was described. The influence of different hydrolysis times on algal digestibility was investigated in brown seaweeds, red seaweeds, and green alga. The highest digestibility values were generally determined in red seaweeds after 24 h of combined hydrolysis by pepsin and pancreatin. Direct dependence between digestion time and digestion efficiency was verified and statistically proven.  相似文献   

Temperature and dietary starch level affected protein but not starch digestibility in gilthead sea bream juveniles     

Couto A  Enes P  Peres H  Oliva-Teles A 《Fish physiology and biochemistry》2012,38(3):595-601

A study was carried out with gilthead sea bream juveniles to assess the effect of water temperature (18 and 25°C) and dietary pregelatinized starch level (10, 20 and 30%) on digestibility of protein and starch and on the activity of proteolytic and amylolytic enzymes. ADC of pregelatinized starch was very high (>99%) irrespectively of dietary inclusion level, and it was not affected by water temperature. ADC of protein was also high (>90%) but improved at the higher water temperature. Dietary starch interacted with protein digestibility, which decreased as dietary starch level increased. Temperature affected both acid and basic protease activities, with acid protease activity being higher at 25°C and basic protease activity being higher at 18°C. However, total proteolytic activity and amylase activities were not affected by water temperature. Dietary carbohydrate exerted no effect on proteolytic or amylolitic activities. It is concluded that gilthead sea bream juveniles digest pregelatinized starch very efficiently irrespective of water temperature, due to adjustments of amylase activity to cope with temperature differences. Pregelatinized starch interacts negatively with protein digestibility, with the ADC of protein decreasing as dietary starch levels increase.  相似文献   

Boron and zinc inhibit embryonic development,hatching and reproduction of Meloidogyne incognita     

Evelyn Aline Arendt Couto  Juliana Kath  Juliana Aparecida Homiak  Heriksen Higashi Puerari 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(4):346-352

Nematodes have difficult control and complex handling, but considering the physiological and biochemical changes that micronutrients promoting in plants, there is possibility that the supply with these chemicals increases the resistance of plants against nematodes. Thus, the present study aimed to assess the effect of the application of boron and zinc on the reproduction of Meloidogyne incognita, embryonic development and juvenile nematode hatching. Nematode reproduction was evaluated in tomato plant inoculated with 2000 eggs and treated in the aerial part with boron or zinc at the following doses: 0, 1/2, 1×, 2× and 4× the manufacturer's recommendation (100 and 30?g/L, respectively), with the plants assessed 60 days after inoculation. For assessment of embryonic development and juvenile hatching, 1?mL nematode suspension was placed in Petri dishes containing 9?mL of the same doses of boron and zinc, and assessment occurred four and eight days after incubation. Results obtained showed that boron controlled nematode population at the dose of 400?g/L and promoted juvenile hatching when used at maximum dosage on the eighth day. Zinc reduced the number of galls and the number of eggs at the dose of 60?g/L, but did not exhibit direct effect on nematode.  相似文献