Screening of pig intestines for K88 non-adhesive phenotype by enzyme immunoassay   总被引：1，自引：0，他引：1  

D S Chandler  H M Chandler  R K Luke  S R Tzipori  J A Craven 《Veterinary microbiology》1986,11(1-2):153-161

An adhesion test for binding of porcine brush border membranes to Escherichia coli cells that possess the K88 antigen (K88+) has been developed using enzyme immunoassay procedures. K88 pilus protein or K88+ E. coli cells were immobilized in the wells of polystyrene microtitre plates. These plates were incubated in the presence of material obtained by scraping the villous surface of pig small intestines. Adhesion of membrane material to immobilized K88 was detected by adding rabbit anti-brush border IgG followed by urease-labelled sheep anti-rabbit IgG conjugate. Action of bound enzyme on urea/bromo-cresol purple substrate solution (pH 4.8) produced an intense colour change from yellow to purple, enabling the test to be read visually. This test enables simple, rapid testing of large numbers of intestial samples and gives results that agree well with the more cumbersome microscopic adhesion test for adhesion of K88+ E. coli to purified brush border membranes.  相似文献   

Establishment and characterization of two novel patient-derived lines from canine high-grade glioma     

Morgan S. Schrock  Abigail A. Zalenski  Miranda M. Tallman  Luke Kollin  Anna Bratasz  Griffin Weeks  Margaret A. Miller  Courtney N. Sweeney  G. Elizabeth Pluhar  Michael R. Olin  William C. Kisseberth  R. Timothy Bentley  Peter J. Dickinson  Daniel York  Amy Webb  Xu Wang  Sarah Moore  Monica Venere  Matthew K. Summers 《Veterinary and comparative oncology》2023,21(3):492-502

High-grade glioma is an aggressive cancer that occurs naturally in pet dogs. Canine high-grade glioma (cHGG) is treated with radiation, chemotherapy or surgery, but has no curative treatment. Within the past eight years, there have been advances in our imaging and histopathology standards as well as genetic charactereization of cHGG. However, there are only three cHGG cell lines publicly available, all of which were derived from astrocytoma and established using methods involving expansion of tumour cells in vitro on plastic dishes. In order to provide more clinically relevant cell lines for studying cHGG in vitro, the goal of this study was to establish cHGG patient-derived lines, whereby cancer cells are expanded in vivo by injecting cells into immunocompromized laboratory mice. The cells are then harvested from mice and used for in vitro studies. This method is the standard in the human field and has been shown to minimize the acquisition of genetic alterations and gene expression changes from the original tumour. Through a multi-institutional collaboration, we describe our methods for establishing two novel cHGG patient-derived lines, Boo-HA and Mo-HO, from a high-grade astrocytoma and a high-grade oligodendroglioma, respectively. We compare our novel lines to G06-A, J3T-Bg, and SDT-3G (traditional cHGG cell lines) in terms of proliferation and sensitivity to radiation. We also perform whole genome sequencing and identify an NF1 truncating mutation in Mo-HO. We report the characterization and availability of these novel patient-derived lines for use by the veterinary community.  相似文献   

The interaction between RUNX2 and core binding factor beta as a potential therapeutic target in canine osteosarcoma     

Fernando Alegre  Amanda R. Ormonde  Dayn R. Godinez  Anuradha Illendula  John H. Bushweller  Luke A. Wittenburg 《Veterinary and comparative oncology》2020,18(1):52-63

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Reproductive interference and Satyrisation: mechanisms,outcomes and potential use for insect control     

Mitchell  Christina  Leigh  Stewart  Alphey  Luke  Haerty  Wilfried  Chapman  Tracey 《Journal of pest science》2022,95(3):1023-1036

Journal of Pest Science - Reproductive Interference occurs when interactions between individuals from different species disrupt reproductive processes, resulting in a fitness cost to one or both...  相似文献   

Plasma metabolomic profiling of healthy pregnant mares and mares with experimentally induced placentitis     

Theresa M. Beachler  Hanna S. Gracz  David R. Morgan  Stasia A. Bembenek Bailey  Luke Borst  Katey E. Ellis  Karen A. Von Dollen  Sara K. Lyle  Amber Nebel  Natalie C. Andrews  Joanna Koipalli  John E. Gadsby  Christopher S. Bailey 《Equine veterinary journal》2021,53(1):85-93

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Probabilistic models of fire occurrence across National Park Service units within the Mojave Desert Network,USA     

Ericka E. Hegeman  Brett G. Dickson  Luke J. Zachmann 《Landscape Ecology》2014,29(9):1587-1600

The frequency and size of wildfires within the Mojave Desert are increasing, possibly due to climate and land cover changes and associated increases in non-native invasive plant biomass, as measured by normalized difference vegetation index (NDVI). These patterns are of particular concern to resource managers in regions where native plant communities are not well adapted to fire. We used an information-theoretic and mixed-model approach to quantify the importance of multiple environmental variables in predicting, separately, the probabilities of occurrence of all fires and the occurrence large (>20 ha) fires in five management units administered by the National Park Service in the Mojave Desert Network and based on fire ignition data obtained for the period 1992–2011. Fire occurrence was strongly associated with areas close to roads, high maximum NDVI values in the year preceding ignition, the desert montane ecological zone, and high topographic roughness. Large fire probability was strongly associated with lightning-caused ignition events, high maximum NDVI values in the spring preceding ignition, high topographic roughness, the middle-elevation shrubland ecological zone, and areas further from roads. Our probabilistic models and maps can be used to explore patterns of fire occurrence based upon variability in NDVI values and to assess the vulnerability of Mojave Desert protected areas to undesirable fire events.  相似文献   

800,000 years of abrupt climate variability   总被引：1，自引：0，他引：1  

Barker S  Knorr G  Edwards RL  Parrenin F  Putnam AE  Skinner LC  Wolff E  Ziegler M 《Science (New York, N.Y.)》2011,334(6054):347-351

We constructed an 800,000-year synthetic record of Greenland climate variability based on the thermal bipolar seesaw model. Our Greenland analog reproduces much of the variability seen in the Greenland ice cores over the past 100,000 years. The synthetic record shows strong similarity with the absolutely dated speleothem record from China, allowing us to place ice core records within an absolute timeframe for the past 400,000 years. Hence, it provides both a stratigraphic reference and a conceptual basis for assessing the long-term evolution of millennial-scale variability and its potential role in climate change at longer time scales. Indeed, we provide evidence for a ubiquitous association between bipolar seesaw oscillations and glacial terminations throughout the Middle to Late Pleistocene.  相似文献   

Plant science. Innate immunity in plants goes to the PUB     

O'Neill LA 《Science (New York, N.Y.)》2011,332(6036):1386-1387

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Carbonyl sulfide-mediated prebiotic formation of peptides     

Leman L  Orgel L  Ghadiri MR 《Science (New York, N.Y.)》2004,306(5694):283-286

Almost all discussions of prebiotic chemistry assume that amino acids, nucleotides, and possibly other monomers were first formed on the Earth or brought to it in comets and meteorites, and then condensed nonenzymatically to form oligomeric products. However, attempts to demonstrate plausibly prebiotic polymerization reactions have met with limited success. We show that carbonyl sulfide (COS), a simple volcanic gas, brings about the formation of peptides from amino acids under mild conditions in aqueous solution. Depending on the reaction conditions and additives used, exposure of alpha-amino acids to COS generates peptides in yields of up to 80% in minutes to hours at room temperature.  相似文献   

Nuclear war. Consequences of regional-scale nuclear conflicts   总被引：1，自引：0，他引：1  

Toon OB  Robock A  Turco RP  Bardeen C  Oman L  Stenchikov GL 《Science (New York, N.Y.)》2007,315(5816):1224-1225

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