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排序方式: 共有356条查询结果,搜索用时 15 毫秒
281.
282.
Trouwborst RE Clement BG Tebo BM Glazer BT Luther GW 《Science (New York, N.Y.)》2006,313(5795):1955-1957
Soluble manganese(III) [Mn(III)] has been thought to disproportionate to soluble Mn(II) and particulate Mn(IV)O2 in natural waters, although it persists as complexes in laboratory solutions. We report that, in the Black Sea, soluble Mn(III) concentrations were as high as 5 micromolar and constituted up to 100% of the total dissolved Mn pool. Depth profiles indicated that soluble Mn(III) was produced at the top of the suboxic zone by Mn(II) oxidation and at the bottom of the suboxic zone by Mn(IV)O2 reduction, then stabilized in each case by unknown natural ligands. We also found micromolar concentrations of dissolved Mn(III) in the Chesapeake Bay. Dissolved Mn(III) can maintain the existence of suboxic zones because it can act as either an electron acceptor or donor. Our data indicate that Mn(III) should be ubiquitous at all water column and sediment oxic/anoxic interfaces in the environment. 相似文献
283.
284.
Martin LG Luther TY Alperin DC Gay JM Hines SA 《Journal of the American Veterinary Medical Association》2008,232(7):1004-1009
OBJECTIVE: To characterize the magnitude and duration of the antibody response against human albumin (HA) in critically ill and healthy dogs. DESIGN: Cohort and cross-sectional study. ANIMALS: Fourteen critically ill dogs that received 25% HA as part of their treatment protocol, 2 healthy dogs with no known previous exposure to HA that received 2 infusions of 25% HA (positive control dogs), and 47 healthy dogs and 21 critically ill dogs with no known exposure to HA (negative control dogs). PROCEDURES: An ELISA to detect IgG against HA was developed. Serum samples were obtained from the critically ill dogs prior to infusion of HA, at the time of hospital discharge, and 4 to 6 weeks and 6 months after HA administration. Serum samples were obtained at 2- to 4-week intervals from both positive control dogs for 101 weeks. A single serum sample was obtained from each of the negative control dogs. RESULTS: All 14 critically ill dogs developed serum IgG against HA. Peak antibody response was detected 4 to 6 weeks after HA administration. In both positive control dogs, IgG against HA was detected 10 days after HA administration and continued past 97 weeks. The peak antibody response was detected at 3 weeks in 1 dog and at 9 weeks in the other. Five of the 68 (7%) negative control dogs had a positive antibody response. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that dogs developed a pronounced IgG response following exposure to HA and that some dogs with no history of HA administration were positive for anti-HA IgG. 相似文献
285.
HE Xiao-long LI Bei FU Shao-yin LIU Yong-bin QI Yun-xia WANG Biao TERI Gele RONG Wei-heng DA Lai 《中国畜牧兽医》2016,43(2):333-339
In order to investigate the methylation patterns on growth differentiation factor 11 (GDF11) gene exon 1 in Ujumqin sheep,we compared the level of methylation of GDF11 gene exon 1 in common Ujumqin sheep (CUS) and multi-vertebrae Ujumqin sheep (MUS) using the bisulfite sequencing PCR (BSP) method.The results showed that the average methylation rate of GDF11 gene exon 1 for MUS was extremely significantly higher than that for CUS (P<0.01),the average methylation rate of common Ujumqin sheep and multi-vertebrae Ujumqin sheep were 0.123 and 0.569.Analysis of 13 CpGs of GDF11 gene exon 1 showed that the CpG_11 and CpG_13 methylation sites had the highest methylation rate with a combined methylation value of 90% for MUS.Our results suggested that differential DNA methylation of GDF11 gene exon 1,in particular at these two sites,might cause the increase in the number of vertebrae in Ujumqin sheep. 相似文献
286.
宁夏枸杞的生物学特性及开发利用前景 总被引:16,自引:0,他引:16
枸杞在我国栽培历史十分悠久,我国最早的诗歌总集《诗经》里就有上山采枸杞的记载。可见,早在2 000多年前的春秋时期,我国人民就知道栽培利用枸杞了。唐宋以来的医书及其他诗文,都有记述。在古代,枸杞主要分布在3个地区,一是甘肃的张掖(古称甘州)一带,产品称"甘枸杞";二是宁夏中宁、中卫等地,产品称"西枸杞";三是天津地区,据说是从宁夏引进的,产品称"津枸杞"。其中宁夏枸杞栽培历史悠久、品质优良、早已畅销全国,成为古代的贡品。如今,宁夏枸杞畅销国内外,成了宁夏的古老名牌产品,其开发利用前景十分广阔。 相似文献
287.
紫茎泽兰的危害特性及研究利用近况 总被引:18,自引:1,他引:18
紫茎泽兰是一种外来恶性杂草,大约解放前后侵入我国,目前已严重阻碍了农业牧业的发展,介绍了紫茎泽兰的生物学特性,基干和花的化学成分、危害机理,探讨了综合利用的可能性,公布了一项取得的阶段性成果。 相似文献
288.
巨紫荆的育苗及栽培技术 总被引:10,自引:0,他引:10
巨紫荆(Cercis giganteanCheng etKeng f.)为苏木科落叶乔木,高可达15 m,属速生植物,寿命长。巨紫荆花的特点是多着生于1 a生的基部和2 a生以上的老枝上,成簇开花,花冠紫红色,花梗细长,为下垂总状花序,形似蝶形;4月前后先叶开放,花期约15 d。花后荚果长6~12 cm,先期绿色,并逐渐 相似文献
289.
Two Methods of Vitrification Followed by In Vitro Culture of the Ovine Ovary: Evaluation of the Follicular Development and Ovarian Extracellular Matrix 下载免费PDF全文
FT Bandeira AA Carvalho SV Castro LF Lima DA Viana JSAM Evangelista MJS Pereira CC Campello JR Figueiredo APR Rodrigues 《Reproduction in domestic animals》2015,50(2):177-185
The aim of this study was to evaluate the influence of two vitrification techniques on the extra cellular matrix (ECM) and ovarian follicular development. The ovarian cortex was fragmented (9 mm3) and divided into six groups, viz. fresh control, cultured control, vitrified by the Ovarian Tissue Cryosystem (OTC) method, conventional solid surface vitrification (SSV) method, OTC/cultured and SSV/cultured. Follicles from all the fragments were analysed for morphology, development and viability. The ECM was evaluated based on the condition of collagen and reticular fibres and the immunolocalization of type I collagen and fibronectin. After 7 days of culture, the tissue vitrified by OTC revealed a higher percentage (p < 0.05) of morphologically normal (30.66%) and viable (60.00%) follicles when compared with those vitrified using the SSV technique (21.33% and 23.00%). In all the fragments cultured, regardless of the vitrification method, a significantly higher percentage of developing follicles was observed when compared with the non‐cultured tissue. Analysis of the type I collagen showed increased immunostaining after the in vitro culture in the vitrified fragments. In conclusion, the OTC is better for preserving the follicular viability and morphology and maintaining the integrity of the extracellular matrix components of the ovine ovary. 相似文献
290.
为了观察铅、镉及其联合对新生大鼠大脑皮质神经细胞的形态学影响,本试验通过对原代培养的新生大鼠大脑皮质神经细胞经提取、纯化和鉴定后,分九组进行单独或联合染毒,染毒时间为12 h,显微镜观察神经细胞形态并测量其胞体短直径和突起长度的变化.结果显示,与对照组相比,各试验组神经细胞密度降低,神经网络减少;各试验组神经细胞胞体短直径显著减小(P<0.05),突起长度显著变短(P<0.05);随铅、镉单剂量作用浓度的增加,神经细胞胞体短直径和突起长度有减小趋势,但组问差异不显著(P>0.05).与相应铅、镉单独染毒组比较.各铅镉联合染毒组神经细胞胞体短直径减小、突起长度变短更加明显,部分组间差异显著(P<0.05).结果表明,铅、镉作用浓度与神经细胞形态改变存在明显的剂量一效应关系,铅镉联合表现协同毒性效应. 相似文献