Antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes     

LIU Ying-ge  QI Hao-wen  LI Huan-zhang  SU Ming-quan  YU Wen-bin  MA Yue-yun 《园艺学报》2004,20(5):745-747

AIM: To observe the antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes. METHODS: Rat thymus lymphocytes were separated by Ficoll-Urografin density gradient centrifugation. Lipofectin was used to introduce antisense, sense and mismatched oligonucleotides for c-myc to rat thymus lymphocytes. The antiproliferative effect was assayed by incorporation of [³H]-TdR and MTS cell proliferation assay. TR-PCR was used to detect the expression of c-myc mRNA. RESULTS: c-myc antisense oligonucleotide inhibited ratthymus lymphocytes proliferation[(0.14±0.03)A vs(0.32±0.16)A,P<0.05],but this ef ect had no relationship with the concentration of c-myc antisense oligonucleot ide.c-myc antisense oligonucleotide decreased the expression of c-myc mRNA in rat thymus lymphocytes. CONCLUSION: c-myc antisense oligonucleotide inhibited rat thymus lymphocyte proliferation.  相似文献   

Proteomic analysis of the effects of tumor necrosis factor-α on endothelial cells     

L&#  Jun-ping  WANG Shu-ren  MA Zeng-chun  WANG Sheng-qi 《园艺学报》2004,20(7):1121-1125

AIM: To investigate the affected proteins by tumor necrosis factor (TNF)-α in endothelial cells, and further explore the potential molecular mechanism of TNF-α on endothelial cells. METHODS: Nitric oxide (NO) production in the cultured human umbilical vein endothelial cells (HUVECs) was measured by a NO assay kit. Proteomic alterations were analyzed using two-dimensional electrophoresis, and peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. RESULTS: NO production in HUVECs decreased significantly after TNF-α treatement. Proteomics analysis showed 21 protein spots were changed including 9 spots that were increased and 11 spots that were decreased after TNF-α stimulation, and 1 spot was only detected in TNF-α activated cell gels. CONCLUSIONS: Decreased the expression of ecNOS by TNF-α might result in decreased NO production. Up-regulated MAP/ERK kinase 3 expression might imply that TNF-α activates the expression of adhesion molecules. Cytoskeletal protein actin is also involved in TNF-α injuried HUVECs. Proteomic analysis can find some clues for identifying new potential target of TNF-α.  相似文献   

Changes of Ca^2＋ activated potassium channels and cellular-proliferation in autogenous vein grafts     

Changes of Ca  SONG Sheng-yun  MA Bao-an  FAN Qing-yu 《园艺学报》2004,20(4):575-579

AIM: To investigate changes of Ca^2＋ activated potassium channels (K_Ca) in autogenous vein grafts. METHODS: The contraction of venous ring was measured by means of perfusion in vitro. The intimal proliferation and proliferation of cultured smooth muscle cells (vascular smooth muscle cells, VSMCs) were observed by the means of computerised image analysis and MTT method, respectively. Furthermore, whole cell mode of patch clamp was used to record K_Ca of VSMCs isolated from autogenous vein grafts. RESULTS: 1 week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P＜0.05, n=8 vs control), and they were more enhanced 4 weeks after vein transplantation (P＜0.01, n=8 vs control). TEA (blocker of Ca^2＋ activated potassium channels) increased MTT A₄₉₀ value of VSMCs from femoral vein in a dose-dependent manner (P＜0.05, n=8). K_Ca current density was significantly attenuated in VSMCs from autogenous vein grafts 1-4 weeks after transplantation (P＜0.05, n=5). CONCLUSION: K_Ca was inhibited in autogenous vein graft, which accounted for vasospasm and intimal proliferation.  相似文献   

Influence of irbesarten on renal hypertrophy in streptozotocin-induced diabetic rats LIU Bi-cheng,LUO Dong-dong,ZHANG Xiao-liang.     

SUN Zi-lin  MA Kun-ling  ZHOU Qiu-gen 《园艺学报》2004,20(11):2086-2089

AIM: To investigate the influence of irbesartan (Irb), a new angiotensin II receptor 1 antagonist, on renal hypertrophy in streptozotocin (STZ)-induced diabetic rats. METHODS: Sprague-Dawley (SD) rats were randomly divided into three groups: normal group (Group N, n=7), diabetic group (Group DN, n=6) and irbesartan treated group (Group DNI, n=7). In the experimental group, after the rats subjected to uninephrectomy, STZ was given by peritoneally injection at bolus dose of 50 mg/kg to induce diabetes. Blood glucose (BG), body weight (BW), urinary albumin excretion (Ualb), 24 hour proteinuria (24 h Upro) were measured at week 4, 8, 12, respectively. By the end of experiment at week 12, creatinine clearance (Ccr), kidney weight (KW), indicator of renal hypertrophy (KW/BW), renal total protein content (RTP), glomerular area (AG) and glomerular volume (VG) as well as glomerular basement membrane (GBM) were determined by semi-quantitative pathology technique. RESULTS: It was showed that there was no significant difference in BG between group DN and DNI, while Irb significantly reduced the increasing of Ualb, 24 h Upro in diabetic rats compared to control group (P＜0.01, respectively). Furthermore, Irb markedly inhibited the increasing of KW, KW/BW, RTP, A_G, V_G in diabetic rats (P＜0.05, P＜0.01, respectively). Of interest, Irb significantly prevented the increasing of GBM in diabetic rats. CONCLUSION: Irb exerts its early renal protective action by reducing proteinuria and inhibiting renal hypertrophy as well as the thickening of GBM.  相似文献   

披碱草和野大麦杂种F1与BC1F1代的生物学及农艺特性研究     

李造哲  于卓  马青枝  王俊杰 《中国草地学报》2004,26(5):9-14

对披碱草和野大麦及其杂种F1与BC1F1代的生物学及农艺特性进行了分析比较。结果表明,亲本野大麦生长发育节律较快,较早地开花结实,果后营养期较长,生育期74d,生长天数达206d,具有很强的分蘖能力;披碱草生长发育节律较慢,生育期124d,生长天数193d;杂种F1的生长天数介于双亲之间,生长动态偏向亲本披碱草,分蘖能力介于双亲之间;BC1F1代生长发育节律较F1代提早,不同株系生长天数不同,生长动态偏向轮回亲本野大麦,分蘖能力有较大的变异。杂种F1代表现出较强的杂种优势,BC1F1代产草量较F1代有所降低,不同株系间存在明显的差异。总体上,BC1F1代的生产性能倾向于轮回亲本野大麦。  相似文献   

大肠杆菌新型菌毛(F1987)的基因定位     

房海  陈翠珍  于凤鸣  张晓君  巩元芳  马建军 《中国兽医学报》2004,24(6):558-560

对动物产肠毒素性大肠杆菌 (ETEC)的一种新型菌毛 (F1987)进行了相应基因的定位研究 ,通过对表达该新型菌毛 (F1987)相应 ETEC菌株的培养、质粒提取、对大肠杆菌受体菌株 DH5α的转化及阳性转化子筛选与相应菌毛表达的检定等 ,初步表明该新型菌毛 (F1987)的基因定位于质粒上  相似文献   

H5N1亚型禽流感病毒核蛋白基因的克隆及分子进化分析     

王振国  金宁一  马鸣潇  金扩世  张洪勇  尹革芬  葛淑敏 《中国预防兽医学报》2004,26(6):436-438

用RT-PCR法,以1株h5N1亚型禽流感病毒RNA为模板,扩增了NP全基因cDNA.将cDNA克隆于pMD18-T载体,并对其进行测序.测序结果表明所克隆的1542个核苷酸的片段包含了核蛋白完整阅读框架,其中编码区为1497个核苷酸,编码498个氨基酸.将其序列与6株A型流感病毒NP基因序列进行比较,各毒株之间NP基因核苷酸序列同源性为92.5%～95.9%,编码的氨基酸同源性为97.0%～98.4%.本研究通过分子进化分析揭示了各毒株间的亲源关系.  相似文献   

Material in the middle ear of dogs having magnetic resonance imaging for investigation of neurologic signs     

M. C. Owen  BVSC    C. R. Lamb  MA  VETMB    D. Lu  BVET. MED.  MVM    M. P. Targett  BA  VETMB  PHD 《Veterinary radiology & ultrasound》2004,45(2):149-155

The aim of this study was to determine the prevalence and potential significance of finding material in the middle ear of dogs having magnetic resonance (MR) imaging. Of 466 MR studies reviewed, an increased signal was identified in the tympanic bulla in 32 (7%) dogs. Cavalier King Charles spaniels, Cocker spaniels, Bulldogs, and Boxers were over-represented compared to the population of dogs having MR imaging. Five (16%) dogs had definite otitis media and one (3%) had a meningioma invading the middle ear. Of the remaining dogs, 13 (41%) had possible otitis media and 13 (41%) had neurologic conditions apparently unrelated to otitis media. The most common appearance of material in the middle ear was isointense in T1-weighted images and hyperintense in T2-weighted images. There was no apparent correlation between the signal characteristics of the material and the diagnosis. Enhanced signal after gadolinium administration was observed affecting the lining of the bulla in dogs with otitis media and in dogs with unrelated neurologic conditions. In dogs without clinical signs of otitis media, finding an increased signal in the middle ear during MR imaging may reflect subclinical otitis media or fluid accumulation unrelated to inflammation. Brachycephalic dogs may be predisposed to this condition.  相似文献   

Patch test reactions to house dust mites in dogs with atopic dermatitis     

S. A. F. Nogueira  S. M. F. Torres  K. Horne  C. Jessen 《Veterinary dermatology》2004,15(Z1):4-4

The purpose of this study was to determine the percentage of dogs with spontaneous atopic dermatitis that show a positive patch test reaction to a commercially available 20% house dust mites mixture containing equal parts of Dermatophagoides farinae and Dermatophagoides pteronyssinus in white petrolatum. In addition, we evaluated whether skin reactions induced after the epicutaneous application of house dust mites were clinically and histologically similar to naturally developed skin lesions of dogs with atopic dermatitis. Furthermore, we investigated if the reactions induced by house dust mites were true allergic reactions by comparing them to atopic lesional skin and to patch test reactions induced by an irritant substance (sodium lauryl sulphate). White petrolatum alone and nonlesional skin sites were used as negative controls. Macroscopic and microscopic evaluations of the patch test and control sites were performed in a blinded fashion at 48 and 72 h after patch test application. Microscopic results were evaluated in a qualitative and quantitative manner. A chi‐square test for homogenicity was used for the quantitative analysis to compare the proportion of each dermal inflammatory cell type among positive histopathological tested sites. P values ≤ 0.05 were considered significant. The study included 12 healthy nonatopic dogs and 13 dogs with nonseasonal atopic dermatitis. None of the nonatopic dogs reacted to house dust mites and white petrolatum. Ten (77%) of the 13 atopic dogs reacted macroscopically and histopathologically to house dust mites. Macroscopic reactions induced by house dust mites were characterized by erythema, oedema and papules. The macroscopic reactions induced by house dust mites were identical to lesional skin in 20% of the dogs and identical to reactions induced by sodium lauryl sulphate in 40% of the dogs. Qualitative histopathological findings showed that the reactions induced by house dust mites were similar to atopic lesional skin in 80% of the dogs and were similar to sodium lauryl sulphate in 20% of the dogs. Quantitative analyses showed that the proportion of neutrophils in reactions induced by sodium lauryl sulphate was significantly higher (P < 0.05) compared to house dust mites reactions, which could be a differentiator factor between an allergic and an irritant reaction. These results showed that the epicutaneous application of house dust mites in dogs with atopic dermatitis induced histopathological lesions similar to spontaneous atopic lesions in dogs. Therefore, this study demonstrated that house dust mites penetrated the skin of dogs with atopic dermatitis and induced an inflammatory response that resembled a true allergic reaction. Funding: Small Companion Animal Grant, University of Minnesota.  相似文献   

江河源区披碱草和星星草混播草地土壤物理性状对牦牛放牧强度的响应   总被引：5，自引：1，他引：5  

董全民  赵新全  马玉寿  代勇  施建军  王启基 《草业科学》2005,22(6):65-70

在江河源区披碱草Elymus natans 星星草Puccinellia tenuflora混播人工草地上研究了牦牛放牧强度对土壤物理性状的影响,2年的试验结果表明:随着放牧强度的增加,不同土壤层含水量均呈降低趋势,土壤容重、土壤坚实度呈增大趋势.相关分析表明, 放牧强度与不同土壤层含水量呈极显著的负相关(P＜0.01), 与土壤容重和坚实度呈极显著的正相关(P＜0.01),而且土壤容重和坚实度均具有累积效应.  相似文献